##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138769_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5913750
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83959670259987	33.0	33.0	33.0	33.0	33.0
2	32.83547004861551	33.0	33.0	33.0	33.0	33.0
3	32.834384612132745	33.0	33.0	33.0	33.0	33.0
4	36.788754512787996	37.0	37.0	37.0	37.0	37.0
5	36.80170771507081	37.0	37.0	37.0	37.0	37.0
6	36.81216402451913	37.0	37.0	37.0	37.0	37.0
7	36.80289004438808	37.0	37.0	37.0	37.0	37.0
8	36.7980929190446	37.0	37.0	37.0	37.0	37.0
9	36.792430014796025	37.0	37.0	37.0	37.0	37.0
10-11	36.771492284929195	37.0	37.0	37.0	37.0	37.0
12-13	36.75952534347918	37.0	37.0	37.0	37.0	37.0
14-15	39.122893172690766	40.0	40.0	40.0	37.0	40.0
16-17	39.08440355104629	40.0	40.0	40.0	37.0	40.0
18-19	39.10984028746566	40.0	40.0	40.0	37.0	40.0
20-21	39.14870254732174	40.0	40.0	40.0	37.0	40.0
22-23	39.16827048956637	40.0	40.0	40.0	37.0	40.0
24-25	39.07644292425417	40.0	40.0	40.0	37.0	40.0
26-27	39.002609232478264	40.0	40.0	40.0	37.0	40.0
28-29	38.9790234093537	40.0	40.0	40.0	37.0	40.0
30-31	38.934032513303784	40.0	40.0	40.0	37.0	40.0
32-33	38.783894443008165	40.0	40.0	40.0	37.0	40.0
34-35	38.86479311139682	40.0	40.0	40.0	37.0	40.0
36-37	38.54490837101831	40.0	37.0	40.0	37.0	40.0
38-39	38.73793002175033	40.0	37.0	40.0	37.0	40.0
40-41	38.74820162794698	40.0	37.0	40.0	37.0	40.0
42-43	38.69935377229095	40.0	37.0	40.0	37.0	40.0
44-45	38.61780490676695	40.0	37.0	40.0	37.0	40.0
46-47	38.541535175633925	40.0	37.0	40.0	37.0	40.0
48-49	38.49218152224557	40.0	37.0	40.0	37.0	40.0
50-51	38.43811029684964	40.0	37.0	40.0	37.0	40.0
52-53	38.380588776221444	40.0	37.0	40.0	37.0	40.0
54-55	38.302016759521415	40.0	37.0	40.0	37.0	40.0
56-57	38.185073629590676	40.0	37.0	40.0	37.0	40.0
58-59	38.05529384761764	40.0	37.0	40.0	37.0	40.0
60-61	37.900601987419925	40.0	37.0	40.0	35.0	40.0
62-63	37.70790863918617	38.5	37.0	40.0	33.0	40.0
64-65	37.48476314100032	37.0	37.0	40.0	33.0	40.0
66-67	37.243739538265665	37.0	37.0	40.0	33.0	40.0
68-69	36.984225170458856	37.0	37.0	40.0	33.0	40.0
70-71	36.72517235274164	37.0	37.0	40.0	33.0	40.0
72-73	36.47001157597639	37.0	37.0	37.0	33.0	40.0
74-75	36.218754356738344	37.0	37.0	37.0	33.0	40.0
76-77	34.54181698531302	35.0	33.0	37.0	30.0	37.0
78-79	35.81505050467038	37.0	37.0	37.0	33.0	37.0
80-81	35.92615960335796	37.0	37.0	37.0	33.0	37.0
82-83	35.849942239828025	37.0	37.0	37.0	33.0	37.0
84-85	35.73772268421618	37.0	37.0	37.0	33.0	37.0
86-87	35.65838142143238	37.0	37.0	37.0	33.0	37.0
88-89	35.58125591727041	37.0	37.0	37.0	33.0	37.0
90-91	35.51291008593725	37.0	35.0	37.0	33.0	37.0
92-93	35.447881479593875	37.0	33.0	37.0	33.0	37.0
94-95	35.402460914880095	37.0	33.0	37.0	33.0	37.0
96-97	35.35988610594886	37.0	33.0	37.0	33.0	37.0
98-99	35.31514200994798	37.0	33.0	37.0	33.0	37.0
100	35.22470364482294	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	5.0
20	19.0
21	84.0
22	336.0
23	915.0
24	2310.0
25	4752.0
26	8738.0
27	14218.0
28	21750.0
29	30964.0
30	43652.0
31	59162.0
32	81016.0
33	111051.0
34	160923.0
35	253118.0
36	511287.0
37	1673204.0
38	2818050.0
39	118195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06799408158952	19.913625026421474	14.434563517226803	23.583817374762205
2	14.729655463961109	20.89704502219404	41.52328049038258	22.85001902346227
3	18.205187606426424	25.916844079266653	30.394371722658025	25.4835965916489
4	12.61875985401929	18.11201303259061	38.67741260049366	30.59181451289644
5	13.59483057022444	37.48440271377139	35.20678885886627	13.713977857137905
6	30.005580215599238	37.50620164870006	18.805072923272036	13.683145212428663
7	26.62153455928979	32.744079475797925	22.812462481504966	17.821923483407314
8	24.60161488057493	36.12482773198055	21.089799196787148	18.183758190657368
9	25.76164024519129	16.921800887761574	21.20158951595857	36.11496935108857
10-11	24.17809342633693	26.736021982667513	28.670750369900656	20.415134221094906
12-13	25.18350454449376	24.378017332487847	28.754123863876558	21.68435425914183
14-15	22.369038258296342	25.69425491439442	26.631528218135703	25.305178609173534
16-17	21.750615091946734	28.783521454238002	27.295193405199747	22.170670048615516
18-19	21.774051997463538	27.920794757979284	29.11419995772564	21.190953286831537
20-21	22.600716833513808	26.980163592801837	29.15190314470099	21.267216428983367
22-23	22.304630157397803	26.9354893670849	28.992956599022996	21.7669238764943
24-25	22.131082443364416	27.05826342155224	28.986937969288405	21.82371616579493
26-27	22.01947009528859	27.239342816993396	28.929886225534784	21.811300862183234
28-29	21.925828397138602	27.253572468749105	28.976817096004492	21.8437820381078
30-31	22.247461993929342	27.104956823964084	28.88521574789903	21.76236543420755
32-33	22.017842660178427	27.28080746317457	28.766928659082897	21.934421217564108
34-35	22.14811464903202	27.367101974534563	28.771636118678913	21.713147257754503
36-37	22.22548579121514	27.31835355165585	28.611154041567104	21.84500661556191
38-39	22.00061691829731	27.396058627160997	28.7287704622542	21.874553992287492
40-41	22.308143415496858	27.252449741630315	28.57641892125938	21.86298792161345
42-43	22.198032446392922	27.379785006520198	28.325174464616083	22.097008082470797
44-45	22.251464884408897	27.48727073518133	28.163173003177384	22.098091377232386
46-47	22.363397917011163	27.5423723939968	27.810901128854066	22.28332856013797
48-49	22.27877605912433	27.692338042139493	27.743789933460672	22.28509596527551
50-51	22.39483736213453	27.77328088932769	27.60137949363087	22.23050225490691
52-53	22.51432989239521	27.665325552266175	27.532413234797477	22.287931320541144
54-55	22.443989288002673	27.60125557460154	27.513905698643843	22.440849438751947
56-57	22.446877403165093	27.733224297861852	27.497047211500604	22.32285108747245
58-59	22.41470037744037	27.749651466898978	27.525115942022264	22.31053221363839
60-61	22.54025685090107	27.74399849561081	27.45519614773184	22.26054850575628
62-63	22.492537818168902	27.795931428278603	27.43137321949238	22.280157534060113
64-65	22.45195422975218	27.730645905009187	27.40299118581648	22.414408679422152
66-67	22.497282179237285	27.74244158481277	27.294094546638764	22.46618168931118
68-69	22.441852632287006	27.733197616436563	27.376022624465442	22.448927126810993
70-71	22.46551779456658	27.56329519135694	27.406313423067573	22.564873591008904
72-73	22.494998868847407	27.581180494264974	27.329286244190605	22.594534392697017
74-75	22.385445082450513	27.7065725413295	27.314863489569202	22.593118886650785
76-77	22.514033114645414	27.649657346642222	27.283529504842612	22.552780033869755
78-79	22.4764300377178	27.655024874505614	27.236893849741474	22.631651238035115
80-81	22.510985519554026	27.69025452444418	27.236475853694852	22.562284102306947
82-83	22.529266291800102	27.661727285964204	27.278915749620296	22.5300906726154
84-85	22.59568568758183	27.664950739070072	27.233883776261912	22.505479797086185
86-87	22.508571653770872	27.686612456156485	27.249688004939376	22.55512788513327
88-89	22.54534487135022	27.665804319760312	27.242285084477192	22.546565724412275
90-91	22.569550515750745	27.67216504539023	27.255101315733825	22.503183123125197
92-93	22.592180877223523	27.626143896174465	27.266071718854633	22.51560350774738
94-95	22.48737042918496	27.70200029180474	27.281082399875185	22.529546879135115
96-97	22.575565077239204	27.751221119564264	27.341889422036044	22.331324381160492
98-99	23.67950206578728	29.696186958742487	27.504080787918035	19.120230187552202
100	22.568517266186138	30.63820878782073	21.35761946510793	25.4356544808852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1500.0
1	1285.5
2	1588.5
3	2285.0
4	3137.5
5	4947.0
6	6454.0
7	7574.5
8	8421.5
9	8570.5
10	8102.0
11	7648.0
12	8536.0
13	11246.0
14	17449.5
15	25854.5
16	32878.5
17	37362.5
18	38353.5
19	36220.5
20	32559.0
21	28368.0
22	25512.5
23	25857.5
24	29132.5
25	35259.5
26	44260.0
27	54462.0
28	65533.5
29	78425.5
30	91621.5
31	104585.5
32	118246.5
33	132377.0
34	145954.0
35	158015.5
36	170932.5
37	183928.5
38	194367.5
39	202991.5
40	211987.0
41	220415.5
42	225949.0
43	231081.0
44	235843.0
45	240807.5
46	244118.5
47	245536.0
48	246295.5
49	243463.0
50	239114.5
51	233276.5
52	224332.0
53	212811.0
54	200416.0
55	186862.0
56	172135.0
57	156258.5
58	139140.5
59	121081.0
60	101390.5
61	82946.0
62	66354.0
63	51319.5
64	38888.5
65	28955.5
66	21773.0
67	16660.5
68	12383.5
69	8794.5
70	6168.0
71	4378.5
72	3077.5
73	2040.5
74	1289.5
75	847.5
76	577.5
77	365.0
78	235.0
79	157.5
80	111.0
81	77.0
82	48.5
83	35.5
84	26.0
85	19.0
86	15.5
87	12.0
88	9.0
89	5.5
90	3.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	6.763897696047348E-4
4	0.02008877615726062
5	0.0010314943986472205
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.455019381440928E-6
22-23	0.0
24-25	0.00778060520084406
26-27	0.05109781627898596
28-29	0.08686735398213985
30-31	0.07672317237226947
32-33	0.03543497063159142
34-35	0.004477067075061434
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.9207814696861217E-4
56-57	8.554734617874263E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	9.752236626709152E-6
72-73	0.011915289865253913
74-75	0.026778558295224176
76-77	0.003865529253043553
78-79	0.0
80-81	0.0
82-83	0.010808525072171891
84-85	0.04875837784299469
86-87	0.03974090985778364
88-89	0.010668055955386509
90-91	1.7174130006876093E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	53.0
20-21	282.0
22-23	696.0
24-25	1969.0
26-27	2484.0
28-29	4738.0
30-31	10632.0
32-33	14266.0
34-35	19619.0
36-37	28043.0
38-39	33920.0
40-41	36341.0
42-43	43573.0
44-45	52401.0
46-47	51685.0
48-49	45255.0
50-51	40216.0
52-53	39229.0
54-55	42064.0
56-57	43301.0
58-59	42723.0
60-61	42069.0
62-63	41746.0
64-65	44532.0
66-67	46754.0
68-69	46608.0
70-71	45797.0
72-73	44182.0
74-75	44672.0
76-77	44927.0
78-79	47462.0
80-81	47238.0
82-83	46401.0
84-85	46314.0
86-87	48496.0
88-89	51582.0
90-91	53494.0
92-93	55301.0
94-95	65795.0
96-97	190425.0
98-99	365670.0
100-101	3940795.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.63490633290603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98809133210753	81.53355858964156
2	5.926433123406339	10.50577689562705
3	1.2174066782846091	3.237141806964318
4	0.3896066283570387	1.381309880444218
5	0.19478714183163265	0.8632470035550619
6	0.09424598059532985	0.5012090197391963
7	0.05473286907954726	0.3395869906937816
8	0.03520610034644589	0.24963915252432972
9	0.02275707561044835	0.18153641386286545
>10	0.07491025232066281	1.0437686800113923
>50	0.0013793408061747567	0.07943228115294303
>100	4.434772542268993E-4	0.0837932857832833
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.381948848023674E-5	0.0	0.0	0.0
3	0.0	3.381948848023674E-5	0.0	0.0	0.0
4	0.0	5.072923272035511E-5	0.0	0.0	0.0
5	0.0	5.072923272035511E-5	0.0	0.0	0.0
6	1.690974424011837E-5	5.072923272035511E-5	0.0	0.0	0.0
7	1.690974424011837E-5	5.072923272035511E-5	0.0	0.0	0.0
8	1.690974424011837E-5	8.454872120059185E-5	0.0	1.690974424011837E-5	0.0
9	1.690974424011837E-5	1.1836820968082858E-4	0.0	3.381948848023674E-5	0.0
10-11	1.690974424011837E-5	1.2682308180088776E-4	0.0	9.300359332065102E-5	0.0
12-13	1.690974424011837E-5	1.3527795392094695E-4	0.0	1.099133375607694E-4	0.0
14-15	1.690974424011837E-5	2.4519129148171633E-4	0.0	1.1836820968082858E-4	1.690974424011837E-5
16-17	1.690974424011837E-5	2.536461636017755E-4	0.0	1.521876981610653E-4	1.690974424011837E-5
18-19	1.690974424011837E-5	2.959205242020715E-4	0.0	1.521876981610653E-4	1.690974424011837E-5
20-21	1.690974424011837E-5	3.381948848023674E-4	0.0	1.521876981610653E-4	1.690974424011837E-5
22-23	1.690974424011837E-5	3.804692454026633E-4	0.0	1.521876981610653E-4	2.5364616360177554E-5
24-25	1.690974424011837E-5	4.2274360600295924E-4	0.0	1.9446205876136124E-4	5.072923272035511E-5
26-27	2.5364616360177554E-5	4.3965335024307757E-4	0.0	2.4519129148171633E-4	5.072923272035511E-5
28-29	3.381948848023674E-5	4.3965335024307757E-4	0.0	3.9737898964278167E-4	5.072923272035511E-5
30-31	3.381948848023674E-5	4.3965335024307757E-4	0.0	9.554005495666878E-4	5.918410484041429E-5
32-33	3.381948848023674E-5	4.3965335024307757E-4	0.0	0.0021729021348552103	8.454872120059185E-5
34-35	3.381948848023674E-5	4.734728387233143E-4	0.0	0.0038807863031071655	1.0145846544071021E-4
36-37	3.381948848023674E-5	5.07292327203551E-4	0.0	0.006045233565842316	1.0145846544071021E-4
38-39	3.381948848023674E-5	5.411118156837878E-4	0.0	0.00964700908898753	1.0145846544071021E-4
40-41	3.381948848023674E-5	5.580215599239062E-4	0.0	0.013443246670894103	1.3527795392094695E-4
42-43	3.381948848023674E-5	6.00295920524202E-4	0.0	0.017383217078841683	1.3527795392094695E-4
44-45	3.381948848023674E-5	6.256605368843796E-4	0.0	0.02173747622067216	1.3527795392094695E-4
46-47	3.381948848023674E-5	6.42570281124498E-4	0.0	0.02673430564362714	1.3527795392094695E-4
48-49	3.381948848023674E-5	6.42570281124498E-4	0.0	0.03187486789262312	1.3527795392094695E-4
50-51	3.381948848023674E-5	7.440287465652082E-4	0.0	0.0368716973155781	1.3527795392094695E-4
52-53	3.381948848023674E-5	7.440287465652082E-4	0.0	0.04159797083069119	1.4373282604100614E-4
54-55	3.381948848023674E-5	7.524836186852674E-4	0.0	0.046383428450644686	1.521876981610653E-4
56-57	3.381948848023674E-5	7.609384908053266E-4	0.0	0.051566265060240965	1.690974424011837E-4
58-59	3.381948848023674E-5	7.609384908053266E-4	0.0	0.05700274783343902	1.690974424011837E-4
60-61	3.381948848023674E-5	7.947579792855633E-4	0.0	0.06234622701331642	1.690974424011837E-4
62-63	3.381948848023674E-5	8.032128514056225E-4	0.0	0.06763897696047347	1.690974424011837E-4
64-65	3.381948848023674E-5	8.454872120059184E-4	0.0	0.0731008243500317	1.690974424011837E-4
66-67	3.381948848023674E-5	8.623969562460368E-4	0.0	0.07885859226379202	1.8600718664130205E-4
68-69	3.381948848023674E-5	8.70851828366096E-4	0.0	0.08456563094483197	1.8600718664130205E-4
70-71	3.381948848023674E-5	8.877615726062143E-4	0.0	0.08920735573874446	2.0291693088142043E-4
72-73	3.381948848023674E-5	8.962164447262735E-4	0.0	0.09278376664552948	2.1982667512153878E-4
74-75	3.381948848023674E-5	9.300359332065103E-4	0.0	0.09567533291058972	2.1982667512153878E-4
76-77	3.381948848023674E-5	9.723102938068061E-4	0.0	0.09873599661805116	2.1982667512153878E-4
78-79	3.381948848023674E-5	9.807651659268653E-4	0.0	0.10081589515958571	2.1982667512153878E-4
80-81	3.381948848023674E-5	9.807651659268653E-4	0.0	0.10144155569647009	2.1982667512153878E-4
82-83	3.381948848023674E-5	9.807651659268653E-4	0.0	0.1019403931515536	2.1982667512153878E-4
84-85	3.381948848023674E-5	9.976749101669837E-4	0.0	0.10212640033819488	2.1982667512153878E-4
86-87	3.381948848023674E-5	9.976749101669837E-4	0.0	0.1022447685478757	2.1982667512153878E-4
88	3.381948848023674E-5	0.0010653138871274572	0.0	0.10227013316423589	2.3673641936165717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8290	0.0	49.77467	1
GTATCAA	15115	0.0	38.230633	1
TCAACGC	18015	0.0	31.553387	4
ATCAACG	18125	0.0	31.433006	3
CAACGCA	18725	0.0	30.37521	5
TATCAAC	19100	0.0	29.985315	2
AACGCAG	19130	0.0	29.799252	6
ACGCAGA	22090	0.0	25.748112	7
CGCAGAG	22390	0.0	25.364887	8
GTGGTAT	3855	0.0	24.982967	1
TGGTATC	3690	0.0	24.592085	2
GCAGAGT	26175	0.0	21.680681	9
GAGTACT	15850	0.0	20.548092	12-13
GTACATG	17920	0.0	19.992786	1
TACATGG	17695	0.0	19.690634	2
CAGAGTA	25380	0.0	19.172775	10-11
ACATGGG	18175	0.0	18.81734	3
GTACTTT	17320	0.0	18.73046	14-15
AGAGTAC	23690	0.0	18.697838	10-11
AGTACTT	16990	0.0	17.317907	12-13
>>END_MODULE