FastQCFastQC Report
Fri 27 May 2016
SRR2138768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4938421
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79000.053.5214271
GTATCAA144800.038.914071
CTTATAC41250.037.7041241
ATCAACG175900.031.477913
TCAACGC182300.030.3700494
TATCAAC188400.029.6829242
CAACGCA186350.029.66115
AACGCAG188900.029.26046
ACGCAGA192100.028.8172177
TATACAC61800.027.7792283
GTGGTAT35650.026.700551
TTATACA61650.026.1238882
TGGTATC34850.025.850252
CGCAGAG220800.025.090748
ACACATC67950.022.8837176
GCAGAGT250750.022.043039
ACATCTC73950.021.1419288
GAGTACT153300.020.74362412-13
TACACAT82000.020.1546425
CAGAGTA245600.019.61641310-11
Produced by FastQC (version 0.11.5)