##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4938421
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89413600015065	33.0	33.0	33.0	33.0	33.0
2	32.94974567781888	33.0	33.0	33.0	33.0	33.0
3	33.61965413641324	33.0	33.0	33.0	33.0	37.0
4	36.78393964386592	37.0	37.0	37.0	37.0	37.0
5	36.7986457614691	37.0	37.0	37.0	37.0	37.0
6	36.802761651953126	37.0	37.0	37.0	37.0	37.0
7	36.796865840316165	37.0	37.0	37.0	37.0	37.0
8	36.78389266528714	37.0	37.0	37.0	37.0	37.0
9	36.78524998172493	37.0	37.0	37.0	37.0	37.0
10-11	36.777752747285014	37.0	37.0	37.0	37.0	37.0
12-13	37.02997607939866	37.0	37.0	37.0	37.0	38.5
14-15	39.09081647757451	40.0	40.0	40.0	37.0	40.0
16-17	39.047667462940076	40.0	40.0	40.0	37.0	40.0
18-19	39.08680527642338	40.0	40.0	40.0	37.0	40.0
20-21	39.130894255699076	40.0	40.0	40.0	37.0	40.0
22-23	39.148348837161635	40.0	40.0	40.0	37.0	40.0
24-25	39.037436131895404	40.0	40.0	40.0	37.0	40.0
26-27	38.96969888426018	40.0	40.0	40.0	37.0	40.0
28-29	38.944508346861454	40.0	40.0	40.0	37.0	40.0
30-31	38.87342353361636	40.0	40.0	40.0	37.0	40.0
32-33	38.743322517304584	40.0	37.0	40.0	37.0	40.0
34-35	38.76156498464113	40.0	37.0	40.0	37.0	40.0
36-37	38.51590243520627	40.0	37.0	40.0	37.0	40.0
38-39	38.666634793835236	40.0	37.0	40.0	37.0	40.0
40-41	38.67788042895201	40.0	37.0	40.0	37.0	40.0
42-43	38.62544067272435	40.0	37.0	40.0	37.0	40.0
44-45	38.536889111103434	40.0	37.0	40.0	37.0	40.0
46-47	38.45909069151838	40.0	37.0	40.0	37.0	40.0
48-49	38.405749462535105	40.0	37.0	40.0	37.0	40.0
50-51	38.33568445990586	40.0	37.0	40.0	37.0	40.0
52-53	38.290408127293446	40.0	37.0	40.0	37.0	40.0
54-55	38.206575398930056	40.0	37.0	40.0	37.0	40.0
56-57	38.07791590277575	40.0	37.0	40.0	37.0	40.0
58-59	37.958139488821175	40.0	37.0	40.0	35.0	40.0
60-61	37.79583434190418	40.0	37.0	40.0	33.0	40.0
62-63	37.61010476384753	38.5	37.0	40.0	33.0	40.0
64-65	37.39280524120632	37.0	37.0	40.0	33.0	40.0
66-67	37.15591354956362	37.0	37.0	40.0	33.0	40.0
68-69	36.9009764161624	37.0	37.0	40.0	33.0	40.0
70-71	36.64881643586663	37.0	37.0	40.0	33.0	40.0
72-73	36.392750670405164	37.0	37.0	37.0	33.0	40.0
74-75	35.94222478181923	37.0	35.0	37.0	33.0	40.0
76-77	34.68830588870316	37.0	33.0	37.0	30.0	37.0
78-79	35.80146620161155	37.0	37.0	37.0	33.0	37.0
80-81	35.88979854967567	37.0	37.0	37.0	33.0	37.0
82-83	35.80832606201047	37.0	37.0	37.0	33.0	37.0
84-85	35.69717671286192	37.0	37.0	37.0	33.0	37.0
86-87	35.618504626780634	37.0	37.0	37.0	33.0	37.0
88-89	35.54545001395643	37.0	37.0	37.0	33.0	37.0
90-91	35.47267219797995	37.0	33.0	37.0	33.0	37.0
92-93	35.40998802762084	37.0	33.0	37.0	33.0	37.0
94-95	35.36638032030346	37.0	33.0	37.0	33.0	37.0
96-97	35.32312145696484	37.0	33.0	37.0	33.0	37.0
98-99	35.27041127960698	37.0	33.0	37.0	33.0	37.0
100	35.17356530377879	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	9.0
20	30.0
21	65.0
22	325.0
23	903.0
24	2233.0
25	4508.0
26	7998.0
27	12976.0
28	19474.0
29	27926.0
30	38293.0
31	51878.0
32	70165.0
33	96919.0
34	139067.0
35	216036.0
36	437028.0
37	1389185.0
38	2306212.0
39	117188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85890244497575	0.0	24.43635232614202	29.704745228882235
2	15.052016973959923	23.909947942526994	38.47370842469243	22.56432665882065
3	16.72158209906081	24.339582102705865	33.005737516855845	25.933098281377482
4	13.048139843898255	23.46390823053027	36.91156792961549	26.576383995955993
5	17.49211433440568	35.83215666803216	32.15306361930435	14.522665378257818
6	27.946503548401402	37.16554339939831	20.14682425819913	14.74112879400116
7	26.16913381827916	32.767275208006765	22.213496986182427	18.85009398753164
8	24.234790836990204	32.0120945541095	21.898092527955797	21.855022080944497
9	23.842013469487515	20.427966752935806	23.479772178192178	32.2502475993845
10-11	24.66999067110722	26.0221232657159	28.20814588306667	21.099740180110203
12-13	25.205192104925843	24.13201507121406	28.163313739351096	22.499479084508998
14-15	22.6350487331882	26.35370698447945	26.420934950665405	24.590309331666944
16-17	21.625738267353068	28.89661493015683	27.254966719119327	22.222680083370776
18-19	21.69161964927656	27.595642412827903	29.360123407866606	21.35261453002893
20-21	22.4200916894385	26.961714865265897	29.321868930414418	21.29632451488118
22-23	22.363979993610673	26.740699150300557	29.09983905174696	21.795481804341808
24-25	22.094792216915078	27.03990454229377	29.284467307204352	21.5808359335868
26-27	21.978044591835214	27.440144491973395	29.08223328373864	21.49957763245275
28-29	21.860992643582442	26.968836986380868	29.330625658707195	21.839544711329502
30-31	22.03598425124079	27.23306057272111	29.112566741648667	21.61838843438943
32-33	22.0461684950682	27.26222980891305	29.081187538656856	21.610414157361895
34-35	22.120611005257594	26.989199678074783	29.096633042368737	21.79355627429889
36-37	21.97284511045111	27.32583103237517	29.006037263748674	21.695286593425045
38-39	21.821626351903	27.23463119383248	29.212096312478636	21.73164614178588
40-41	22.13597769020584	26.96341696469445	28.89957093523613	22.001034409863582
42-43	22.099943192896852	27.003091302698074	28.866845755115012	22.030119749290066
44-45	22.0455238662599	27.445268716010712	28.539495000942782	21.969712416786606
46-47	22.3760495775155	27.15830944212983	28.036744354339938	22.428896626014737
48-49	22.239646296994923	27.357579076199833	28.120421494081565	22.282353132723674
50-51	22.222272611883675	27.71531079425372	27.80555867791493	22.25685791594767
52-53	22.381506056061678	27.54199992887313	27.866094853013706	22.210399162051488
54-55	22.23124426086769	27.588439748163506	27.88142652710908	22.298889463859723
56-57	22.405614859333	28.00312992261031	27.352112206156864	22.239143011899827
58-59	22.295189027563712	27.845020021107697	27.742691153378512	22.11709979795008
60-61	22.426650465037802	28.046011366553646	27.475918237548846	22.051419930859705
62-63	22.262635719104917	28.244651360035817	27.399776071914744	22.09293684894452
64-65	22.365392769844096	27.914108814093613	27.397634762922213	22.32286365314008
66-67	22.360020645867866	27.91168480271726	27.384912236561686	22.343382314853187
68-69	22.429012664824107	27.833613456178448	27.46799910500897	22.269374773988478
70-71	22.457951525787063	27.496371948478952	27.490131534710034	22.55554499102395
72-73	22.412974827631647	27.55318894399899	27.50987142947441	22.52396479889496
74-75	22.313594002321764	27.742396433906425	27.456391232632697	22.487618331139114
76-77	22.551057437686328	27.527269709161473	27.32794108470588	22.593731768446318
78-79	22.371862076589686	27.621063429169396	27.447435651757807	22.559638842483114
80-81	22.445354995546595	27.715120785380375	27.338421364891346	22.50110285418168
82-83	22.57399225086635	27.4810824836148	27.41611550350657	22.52880976201228
84-85	22.588586453657552	27.522898324496246	27.46198649430744	22.426528727538763
86-87	22.46185622638484	27.55627868975549	27.465375036428913	22.51649004743076
88-89	22.591980627629113	27.368541272973534	27.408977547449926	22.63050055194743
90-91	22.623657486630982	27.410414854994436	27.46713486154216	22.498792796832422
92-93	22.581846747336314	27.445286663164786	27.488120571392745	22.48474601810615
94-95	22.582928918679887	27.357940537898408	27.48047290553075	22.57865763789096
96-97	22.66355755353923	27.62643822970449	27.603437740909996	22.106566475846282
98-99	23.700771625240943	29.71849498678956	27.11920843537382	19.46152495259568
100	22.8373780911419	29.95112303976016	21.50916889944372	25.702329969654226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	889.0
1	842.0
2	1269.5
3	1851.0
4	2640.0
5	4551.5
6	6143.0
7	7297.0
8	8179.0
9	8329.0
10	7815.0
11	7218.0
12	7845.0
13	10135.0
14	16037.5
15	24875.0
16	32947.0
17	38060.5
18	38865.0
19	35869.0
20	31151.0
21	26270.5
22	22889.5
23	22458.5
24	24854.0
25	30078.5
26	37531.5
27	45959.0
28	54874.5
29	65286.5
30	75685.0
31	85685.0
32	96413.5
33	107603.0
34	119244.5
35	129579.0
36	140276.0
37	151123.5
38	159309.0
39	165728.5
40	173372.5
41	180166.5
42	184279.0
43	188938.5
44	194505.0
45	200998.5
46	207581.0
47	212914.0
48	213808.0
49	210143.0
50	206049.0
51	199331.5
52	188823.5
53	177930.5
54	167149.0
55	155384.5
56	143284.5
57	129902.0
58	115367.5
59	100640.0
60	84997.0
61	69893.0
62	55920.5
63	43165.0
64	32411.5
65	23927.5
66	17880.0
67	13662.5
68	10301.5
69	7324.5
70	5048.0
71	3468.5
72	2303.5
73	1467.0
74	926.5
75	609.0
76	399.0
77	268.5
78	189.5
79	128.0
80	89.0
81	61.5
82	40.5
83	26.0
84	19.0
85	14.5
86	12.0
87	10.5
88	7.5
89	3.5
90	0.5
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6199509924326013E-4
2	6.68229784378448E-4
3	0.004495364004000468
4	0.015065544229623192
5	6.074816221622255E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.037481304302572E-5
22-23	5.16408840271303E-4
24-25	0.012487760374361383
26-27	0.05513637031863898
28-29	0.08327721540455138
30-31	0.06961174730458726
32-33	0.03174957658756527
34-35	0.003630297072512125
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.0875667412519937E-5
54-55	3.506055889817811E-4
56-57	6.738810977677744E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.15624246635768E-5
68-69	3.505185571534528E-5
70-71	0.0012622227695786381
72-73	0.015496197897158805
74-75	0.02455433875165742
76-77	0.0030169187348714852
78-79	7.337413016497317E-5
80-81	9.015412032117097E-4
82-83	0.015603112690020283
84-85	0.04782201394533756
86-87	0.03646015411475611
88-89	0.009040754124924446
90-91	1.1710930514104646E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	63.0
20-21	287.0
22-23	664.0
24-25	1886.0
26-27	2305.0
28-29	4503.0
30-31	9258.0
32-33	12378.0
34-35	17671.0
36-37	24069.0
38-39	28216.0
40-41	30572.0
42-43	38122.0
44-45	46247.0
46-47	44212.0
48-49	38791.0
50-51	33609.0
52-53	33201.0
54-55	36250.0
56-57	39475.0
58-59	40064.0
60-61	39265.0
62-63	39780.0
64-65	41283.0
66-67	46355.0
68-69	41525.0
70-71	38245.0
72-73	36582.0
74-75	37526.0
76-77	37629.0
78-79	39528.0
80-81	40414.0
82-83	38774.0
84-85	39249.0
86-87	41580.0
88-89	44572.0
90-91	46338.0
92-93	48680.0
94-95	62422.0
96-97	228642.0
98-99	867168.0
100-101	2601021.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.3548761125663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.59920390877706	80.93236313369697
2	6.324788439978815	11.176517981050395
3	1.2574600117127306	3.3330817065415346
4	0.38724948389867037	1.3686152069808906
5	0.17016377891914014	0.7517399802623376
6	0.08633511972881718	0.4576877284681933
7	0.047956962660083265	0.2966062046196637
8	0.030609794979938793	0.21636197146268368
9	0.02045723467973249	0.16267467921601134
>10	0.07333104765198788	1.013117698323534
>50	0.0014703417826591179	0.08832363308823972
>100	9.406497953613167E-4	0.18682282831158986
>500	3.3225434998281456E-5	0.01608724797796487
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.0249387405407516E-5
7	0.0	0.0	0.0	0.0	2.0249387405407516E-5
8	0.0	4.049877481081503E-5	0.0	0.0	2.0249387405407516E-5
9	0.0	1.0124693702703759E-4	0.0	0.0	2.0249387405407516E-5
10-11	0.0	1.0124693702703759E-4	0.0	0.0	2.0249387405407516E-5
12-13	0.0	1.0124693702703759E-4	0.0	0.0	2.0249387405407516E-5
14-15	0.0	1.8224448664866765E-4	0.0	0.0	2.0249387405407516E-5
16-17	0.0	2.0249387405407517E-4	0.0	0.0	2.0249387405407516E-5
18-19	0.0	2.0249387405407517E-4	0.0	0.0	2.0249387405407516E-5
20-21	0.0	2.0249387405407517E-4	0.0	2.0249387405407516E-5	2.0249387405407516E-5
22-23	0.0	2.6324203627029776E-4	0.0	7.08728559189263E-5	3.0374081108111273E-5
24-25	0.0	3.239901984865203E-4	0.0	9.112224332433383E-5	4.049877481081503E-5
26-27	0.0	3.644889732973353E-4	0.0	2.0249387405407517E-4	4.049877481081503E-5
28-29	0.0	3.644889732973353E-4	0.0	5.973569284595218E-4	4.049877481081503E-5
30-31	0.0	4.657359103243729E-4	0.0	0.0013769583435677113	4.049877481081503E-5
32-33	0.0	4.7586060402707666E-4	0.0	0.00285516362416246	4.049877481081503E-5
34-35	0.0	5.771075410541143E-4	0.0	0.00530533950021677	4.049877481081503E-5
36-37	0.0	6.68229784378448E-4	0.0	0.008535116791379268	4.049877481081503E-5
38-39	0.0	6.68229784378448E-4	0.0	0.01378983282308252	6.074816221622255E-5
40-41	0.0	7.492273340000781E-4	0.0	0.019267292116245255	6.074816221622255E-5
42-43	0.0	8.302248836217082E-4	0.0	0.024765000796813395	6.074816221622255E-5
44-45	0.0	9.112224332433383E-4	0.0	0.031123308442111353	1.0124693702703759E-4
46-47	0.0	9.112224332433383E-4	0.0	0.03781573097959854	1.0124693702703759E-4
48-49	0.0	9.314718206487458E-4	0.0	0.044447405354869504	1.1137163072974135E-4
50-51	0.0	0.0011339656947028211	0.0	0.05096770809941072	1.214963244324451E-4
52-53	0.0	0.0011542150821082285	0.0	0.057336140438411384	1.214963244324451E-4
54-55	0.0	0.0011542150821082285	0.0	0.06360332584038501	1.4174571183785264E-4
56-57	0.0	0.0011643397758109323	0.0	0.06909090982725045	1.4174571183785264E-4
58-59	0.0	0.0011947138569190436	0.0	0.07450762095819696	1.4174571183785264E-4
60-61	0.0	0.0012250879380271548	0.0	0.0797623369899002	1.4174571183785264E-4
62-63	0.0	0.0012352126317298586	0.0	0.08490568139087373	1.4174571183785264E-4
64-65	0.0	0.0013769583435677111	0.0	0.08932004784525256	1.4174571183785264E-4
66-67	0.0	0.0014174571183785262	0.0	0.0942912724532801	1.4174571183785264E-4
68-69	0.0	0.00142758181208123	0.0	0.09944474154795632	1.4174571183785264E-4
70-71	0.0	0.0014377065057839338	0.0	0.10427422044414601	2.0249387405407517E-4
72-73	0.0	0.0014377065057839338	0.0	0.10819247690709237	2.0249387405407517E-4
74-75	0.0	0.0014579558931893414	0.0	0.11128050848641702	2.0249387405407517E-4
76-77	0.0	0.00150857936170286	0.0	0.11458115863349844	2.0249387405407517E-4
78-79	2.0249387405407516E-5	0.0015389534428109713	0.0	0.11630235656295808	2.0249387405407517E-4
80-81	2.0249387405407516E-5	0.001549078136513675	0.0	0.11685921471660679	2.0249387405407517E-4
82-83	2.0249387405407516E-5	0.001559202830216379	0.0	0.11732495062693116	2.0249387405407517E-4
84-85	2.0249387405407516E-5	0.0015693275239190827	0.0	0.11745657164506632	2.0249387405407517E-4
86-87	2.0249387405407516E-5	0.0015794522176217863	0.0	0.11751731980728253	2.0249387405407517E-4
88	2.0249387405407516E-5	0.001640200379838009	0.0	0.11752744450098523	2.0249387405407517E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7900	0.0	53.521427	1
GTATCAA	14480	0.0	38.91407	1
CTTATAC	4125	0.0	37.704124	1
ATCAACG	17590	0.0	31.47791	3
TCAACGC	18230	0.0	30.370049	4
TATCAAC	18840	0.0	29.682924	2
CAACGCA	18635	0.0	29.6611	5
AACGCAG	18890	0.0	29.2604	6
ACGCAGA	19210	0.0	28.817217	7
TATACAC	6180	0.0	27.779228	3
GTGGTAT	3565	0.0	26.70055	1
TTATACA	6165	0.0	26.123888	2
TGGTATC	3485	0.0	25.85025	2
CGCAGAG	22080	0.0	25.09074	8
ACACATC	6795	0.0	22.883717	6
GCAGAGT	25075	0.0	22.04303	9
ACATCTC	7395	0.0	21.141928	8
GAGTACT	15330	0.0	20.743624	12-13
TACACAT	8200	0.0	20.154642	5
CAGAGTA	24560	0.0	19.616413	10-11
>>END_MODULE