FastQCFastQC Report
Fri 27 May 2016
SRR2138767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6925952
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC117900.054.2175251
GGTATCA140800.046.2925831
TATACAC149500.043.9350853
TTATACA155800.041.1361582
GTATCAA256100.036.0405461
ATACACA194700.034.683984
ACACATC200350.032.2255026
ATCAACG303150.030.0597693
TACACAT223450.029.4352025
ACGCAGA316400.028.7943487
AACGCAG317600.028.6449176
TATCAAC320300.028.5549532
TCAACGC319700.028.5046844
CAACGCA321050.028.3472925
ACATCTC241550.026.8340648
CACATCT252700.025.500737
CGCAGAG364600.025.0222428
CGCTACG2201.9970491E-423.21593994
CATCTCC280700.023.0167629
GCAGAGT411250.022.1532699
Produced by FastQC (version 0.11.5)