##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6925952
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.772517626457706	33.0	33.0	33.0	33.0	33.0
2	32.78919851018315	33.0	33.0	33.0	33.0	33.0
3	33.54749527573971	33.0	33.0	33.0	33.0	37.0
4	36.704141322377055	37.0	37.0	37.0	37.0	37.0
5	36.709060357334266	37.0	37.0	37.0	37.0	37.0
6	36.687739100704135	37.0	37.0	37.0	37.0	37.0
7	36.68533423275241	37.0	37.0	37.0	37.0	37.0
8	36.66243470933671	37.0	37.0	37.0	37.0	37.0
9	36.678346601304774	37.0	37.0	37.0	37.0	37.0
10-11	36.693022128943426	37.0	37.0	37.0	37.0	37.0
12-13	36.949864581793236	37.0	37.0	37.0	37.0	38.5
14-15	38.903228899074094	40.0	38.5	40.0	37.0	40.0
16-17	38.82358945023009	40.0	37.0	40.0	37.0	40.0
18-19	38.86496506184276	40.0	37.0	40.0	37.0	40.0
20-21	38.88195255953164	40.0	37.0	40.0	37.0	40.0
22-23	38.884657146663216	40.0	37.0	40.0	37.0	40.0
24-25	38.86750117085218	40.0	37.0	40.0	37.0	40.0
26-27	38.775690978861476	40.0	37.0	40.0	37.0	40.0
28-29	38.6521771606901	40.0	37.0	40.0	37.0	40.0
30-31	38.50979500909425	40.0	37.0	40.0	37.0	40.0
32-33	38.39221852751385	40.0	37.0	40.0	37.0	40.0
34-35	38.35482123464028	40.0	37.0	40.0	37.0	40.0
36-37	38.3408356189801	40.0	37.0	40.0	37.0	40.0
38-39	38.23369958001899	40.0	37.0	40.0	37.0	40.0
40-41	38.25206526506123	40.0	37.0	40.0	37.0	40.0
42-43	38.233153023241144	40.0	37.0	40.0	37.0	40.0
44-45	38.0983401049123	40.0	37.0	40.0	37.0	40.0
46-47	37.81728727245407	40.0	37.0	40.0	33.0	40.0
48-49	37.96588331761813	40.0	37.0	40.0	33.0	40.0
50-51	37.89550118762703	40.0	37.0	40.0	33.0	40.0
52-53	37.840526563913016	40.0	37.0	40.0	33.0	40.0
54-55	37.722725059972944	40.0	37.0	40.0	33.0	40.0
56-57	37.51913264519883	40.0	37.0	40.0	33.0	40.0
58-59	37.34736077754637	38.5	37.0	40.0	33.0	40.0
60-61	37.149822476607454	37.0	37.0	40.0	33.0	40.0
62-63	36.98869504466565	37.0	37.0	40.0	33.0	40.0
64-65	36.83211710635851	37.0	37.0	40.0	33.0	40.0
66-67	36.63714729203347	37.0	37.0	40.0	33.0	40.0
68-69	36.44826757453808	37.0	37.0	40.0	33.0	40.0
70-71	36.231455726052246	37.0	37.0	38.5	33.0	40.0
72-73	35.94192644322777	37.0	37.0	37.0	33.0	40.0
74-75	35.35654253046669	37.0	33.0	37.0	33.0	40.0
76-77	33.745480681409134	35.0	33.0	37.0	30.0	37.0
78-79	35.221862698377194	37.0	33.0	37.0	33.0	37.0
80-81	35.37861770908761	37.0	33.0	37.0	33.0	37.0
82-83	35.21617719601969	37.0	33.0	37.0	33.0	37.0
84-85	35.075660717584924	37.0	33.0	37.0	33.0	37.0
86-87	34.98602952323402	37.0	33.0	37.0	33.0	37.0
88-89	34.80645941133736	37.0	33.0	37.0	33.0	37.0
90-91	34.768969592659445	37.0	33.0	37.0	33.0	37.0
92-93	34.805510217177826	37.0	33.0	37.0	33.0	37.0
94-95	34.798011706166776	37.0	33.0	37.0	33.0	37.0
96-97	34.791118592556714	37.0	33.0	37.0	33.0	37.0
98-99	34.786227952382134	37.0	33.0	37.0	33.0	37.0
100	34.71908287143184	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	5.0
19	16.0
20	35.0
21	143.0
22	595.0
23	1731.0
24	4184.0
25	8835.0
26	15449.0
27	25712.0
28	37954.0
29	53935.0
30	72425.0
31	97214.0
32	132514.0
33	184106.0
34	264906.0
35	417380.0
36	839224.0
37	2235061.0
38	2432487.0
39	102038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.66647742662809	0.026292429570652278	24.92219115708059	30.385038986720662
2	14.791820707373695	24.01924709306145	39.16245748404293	22.026474715521918
3	16.03278741071082	24.39962396499773	33.733981080720895	25.83360754357055
4	12.424012240340712	24.39470045341066	36.70667031863206	26.47461698761657
5	16.731998248327198	35.46900635017487	32.858924355658885	14.940071045839048
6	26.900497404688167	38.211725467264415	19.666298486128255	15.221478641919159
7	25.038395449231448	32.80000103956843	23.02130061272452	19.140302898475603
8	23.845865410174234	32.11679211521544	21.7940737923637	22.24326868224663
9	23.292260689523733	20.39287085940681	23.710601015890983	32.60426743517847
10-11	24.1552654189108	26.047503512440294	28.325324923739203	21.471906144909703
12-13	24.76934469714992	24.176666825742192	28.294431906517303	22.759556570590583
14-15	22.31608737686891	26.05985429873034	26.45266672365041	25.171391600750336
16-17	21.25761916917703	28.971576759411562	27.42470637971502	22.34609769169639
18-19	21.053011918072777	27.349965752000593	29.672592302112406	21.924430027814225
20-21	21.886252270623608	26.90714153855239	29.729605827466465	21.477000363357533
22-23	21.71121761406068	26.971561103522152	29.57858088770819	21.738640394708977
24-25	21.83823325499005	27.320262542435948	29.81546863842149	21.02603556415252
26-27	21.86681443549226	27.888485591094568	29.6357965645193	20.608903408893873
28-29	21.031696601787857	27.14905825877296	29.84785686085679	21.97138827858239
30-31	21.900990695533306	27.49805947243575	29.803795084634082	20.797154747396863
32-33	21.55645980285318	27.7226364087253	29.875193688188318	20.845710100233205
34-35	21.494868268062326	27.251922578163473	30.172529352282762	21.080679801491435
36-37	21.69122740710614	27.078003170706484	29.97031954664211	21.26044987554527
38-39	21.054830755343605	27.033525961868115	30.291792705454696	21.619850577333583
40-41	21.308591727252015	26.824580636952476	30.005370569280345	21.861457066515165
42-43	21.923580886515012	26.592349880192938	29.867011818470235	21.617057414821815
44-45	21.296942315950123	27.61995798512874	29.51358577459479	21.569513924326344
46-47	21.845927774538424	26.802300796821033	28.57434555753546	22.777425871105088
48-49	21.77921246619858	26.898592536792936	29.062318178642354	22.259876818366127
50-51	21.45300214739484	27.452227435359823	28.53061798531703	22.564152431928306
52-53	21.683242338737355	27.634692408583362	28.83898718036718	21.843078072312103
54-55	21.62292516738855	27.967234236861245	28.445436088297647	21.96440450745256
56-57	22.16865274758114	28.73156620877119	27.06075561281487	22.039025430832798
58-59	21.524727233582276	28.63275373821177	28.480893540161915	21.36162548804404
60-61	21.985508455544352	29.26460548053859	27.273919062057267	21.475967001859793
62-63	21.471915524304446	29.66592805145295	27.217034329649703	21.6451220945929
64-65	21.652359298764644	29.14240726318898	27.331121090549104	21.87411234749727
66-67	21.779910890039513	28.881469830959418	27.365773277621187	21.972846001379885
68-69	21.930469014197982	28.405309647623984	27.592898050379798	22.071323287798233
70-71	22.186712017767327	27.585456747586214	27.72130212297385	22.50652911167261
72-73	22.097585610359204	27.838528706728184	27.676410199552077	22.38747548336054
74-75	22.042934256792172	27.959015604104486	27.661239568575812	22.33681057052753
76-77	22.235812404135448	27.71670884021723	27.44366801581517	22.60381073983215
78-79	22.175217486808865	27.78582055614977	27.504433158372432	22.53452879866893
80-81	22.308043660881204	27.798973044822173	27.3812734225953	22.51170987170133
82-83	22.52286148556051	27.284639684161277	27.62252547317207	22.56997335710614
84-85	22.48138352379036	27.441804070543736	27.60745096214941	22.46936144351649
86-87	22.248498287833772	27.679145263603	27.480147285192295	22.59220916337093
88-89	22.544773859336058	27.439786217032548	27.404530605210457	22.610909318420934
90-91	22.576802484022704	27.38725414142571	27.567988784849028	22.467954589702558
92-93	22.511798901043484	27.53675136430201	27.494737091908323	22.456712642746187
94-95	22.635518013996776	27.169980611724	27.574916449355356	22.61958492492387
96-97	22.65457667408326	27.492952517473686	27.75053432988973	22.101936478553327
98-99	23.683999716189337	29.693267049811062	27.134027296177027	19.488705937822576
100	22.962288305505176	29.66540987341691	21.496750968625797	25.875550852452122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1529.0
1	1566.0
2	3008.0
3	4701.0
4	6984.0
5	12679.0
6	16960.5
7	18787.5
8	19249.0
9	17758.0
10	15467.5
11	13249.5
12	14298.0
13	19182.5
14	31277.5
15	50248.0
16	67297.5
17	77311.5
18	78221.5
19	71603.0
20	61462.0
21	50272.0
22	42121.0
23	39126.5
24	40955.5
25	48731.0
26	60494.5
27	73585.0
28	85503.5
29	97850.5
30	110231.0
31	121149.5
32	135053.0
33	150869.5
34	165885.0
35	182239.0
36	204252.0
37	220528.5
38	222280.5
39	224893.0
40	237113.0
41	246984.5
42	249086.0
43	254555.5
44	264121.5
45	274859.5
46	294349.0
47	312963.0
48	332127.0
49	333942.5
50	315994.5
51	300644.5
52	284143.0
53	260232.0
54	228437.5
55	201950.5
56	180108.0
57	157170.5
58	134917.0
59	115327.5
60	94681.5
61	73982.5
62	56206.5
63	41446.5
64	29525.5
65	21262.0
66	16014.5
67	12280.0
68	9052.5
69	6549.5
70	4910.0
71	3601.0
72	2661.5
73	2024.0
74	1486.0
75	1002.5
76	673.0
77	434.0
78	287.5
79	214.5
80	162.0
81	117.0
82	93.0
83	68.5
84	45.0
85	30.0
86	21.5
87	16.0
88	11.5
89	9.5
90	8.5
91	7.0
92	4.5
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.775379326914192E-5
2	3.32084311297566E-4
3	1.443844831728548E-5
4	1.443844831728548E-5
5	1.443844831728548E-5
6	2.887689663457096E-5
7	1.443844831728548E-5
8	7.21922415864274E-5
9	1.7326137980742574E-4
10-11	0.003053731819105879
12-13	0.007515212349147092
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	3.6207236899751E-5
32-33	6.098948911500984E-4
34-35	0.01084656452733418
36-37	0.062274557361677016
38-39	0.1652372970567157
40-41	0.16756752365626576
42-43	0.049315641433670204
44-45	0.00411937591904213
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.144003783494153E-5
58-59	7.150095763615927E-5
60-61	0.0015110871194773182
62-63	0.024476105923990906
64-65	0.11746368231561734
66-67	0.23135553593857136
68-69	0.23447687168944342
70-71	0.09802783675517647
72-73	0.00660646586627218
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	175.0
20-21	567.0
22-23	1327.0
24-25	2344.0
26-27	3840.0
28-29	8832.0
30-31	17446.0
32-33	21029.0
34-35	27420.0
36-37	30430.0
38-39	32584.0
40-41	40426.0
42-43	49007.0
44-45	61575.0
46-47	60480.0
48-49	48391.0
50-51	41543.0
52-53	41691.0
54-55	58024.0
56-57	66347.0
58-59	72407.0
60-61	76673.0
62-63	82019.0
64-65	91282.0
66-67	104298.0
68-69	90747.0
70-71	66447.0
72-73	59459.0
74-75	59984.0
76-77	56276.0
78-79	58813.0
80-81	60682.0
82-83	63908.0
84-85	68951.0
86-87	70992.0
88-89	76623.0
90-91	76382.0
92-93	82545.0
94-95	100617.0
96-97	316336.0
98-99	1158014.0
100-101	3419019.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.48912554864606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.59469707071699	63.758741363989635
2	8.539415435210103	12.721871769308226
3	2.733187917604693	6.107783338274952
4	1.2555824886813238	3.7410896654425834
5	0.6284453098051421	2.3406170791266496
6	0.3746078148316826	1.6742525130300692
7	0.2410708491454717	1.2570009723681026
8	0.1510618855950369	0.9001974209363127
9	0.10448149312565116	0.7004461553051965
>10	0.3554036836472538	4.106368354566492
>50	0.010668149386646357	0.5452920839868459
>100	0.010411542427955103	1.5199330462428602
>500	7.120546058278952E-4	0.3810568486795869
>1k	2.5430521635693283E-4	0.24534938874246656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.443844831728548E-5
2	0.0	0.0	0.0	0.0	1.443844831728548E-5
3	0.0	0.0	0.0	0.0	1.443844831728548E-5
4	0.0	0.0	0.0	0.0	1.443844831728548E-5
5	0.0	0.0	0.0	0.0	1.443844831728548E-5
6	0.0	0.0	0.0	0.0	1.443844831728548E-5
7	0.0	0.0	0.0	0.0	1.443844831728548E-5
8	0.0	0.0	0.0	0.0	1.443844831728548E-5
9	0.0	0.0	0.0	0.0	1.443844831728548E-5
10-11	0.0	0.0	0.0	1.443844831728548E-5	1.443844831728548E-5
12-13	0.0	0.0	0.0	1.443844831728548E-5	4.331534495185644E-5
14-15	0.0	0.0	0.0	5.775379326914192E-5	6.497301742778466E-5
16-17	0.0	0.0	0.0	1.1550758653828383E-4	1.4438448317285479E-4
18-19	0.0	0.0	0.0	5.919763810087046E-4	1.4438448317285479E-4
20-21	0.0	0.0	0.0	0.0012489257794451938	1.66042155648783E-4
22-23	0.0	0.0	0.0	0.0020863557818477517	1.7326137980742576E-4
24-25	0.0	0.0	0.0	0.0038334080282392945	1.7326137980742576E-4
26-27	0.0	0.0	0.0	0.008056654161045299	1.8048060396606852E-4
28-29	0.0	0.0	0.0	0.024343223862943318	1.9491905228335397E-4
30-31	0.0	0.0	0.0	0.031129294572067492	2.0213827644199672E-4
32-33	0.0	0.0	0.0	0.03330228104381895	2.0935750060063947E-4
34-35	0.0	0.0	0.0	0.036132216914006914	2.4545362139385317E-4
36-37	0.0	0.0	0.0	0.039936748045611634	2.4545362139385317E-4
38-39	0.0	0.0	0.0	0.04966826221146205	2.4545362139385317E-4
40-41	0.0	0.0	0.0	0.05863453861649633	2.4545362139385317E-4
42-43	0.0	0.0	0.0	0.0641861219944926	2.4545362139385317E-4
44-45	0.0	0.0	0.0	0.07074839675469885	2.5267284555249587E-4
46-47	0.0	0.0	0.0	0.07773660574026503	2.598920697111386E-4
48-49	0.0	0.0	0.0	0.0855694639523924	3.03207414662995E-4
50-51	0.0	0.0	0.0	0.09435525975346061	3.32084311297566E-4
52-53	0.0	0.0	0.0	0.11859019525402428	3.60961207932137E-4
54-55	0.0	0.0	0.0	0.1505857967251289	3.60961207932137E-4
56-57	0.0	0.0	0.0	0.18439342346005286	3.6818043209077973E-4
58-59	0.0	0.0	0.0	0.20784146352732447	3.753996562494225E-4
60-61	0.0	0.0	0.0	0.22433739072982314	3.826188804080652E-4
62-63	0.0	0.0	0.0	0.23980096887763588	4.187150012012789E-4
64-65	0.0	0.0	0.0	0.24607447467149643	4.403726736772071E-4
66-67	0.0	0.0	0.0	0.2527883531390342	4.4759189783584983E-4
68-69	0.0	0.0	0.0	0.2601447425566911	4.4759189783584983E-4
70-71	0.0	0.0	0.0	0.26524151481269287	4.6203034615313534E-4
72-73	0.0	0.0	0.0	0.26911823818588404	4.7646879447042084E-4
74-75	0.0	0.0	0.0	0.272150312332514	4.836880186290636E-4
76-77	0.0	0.0	0.0	0.2750524404442884	4.909072427877063E-4
78-79	0.0	0.0	0.0	0.27651794294849286	4.909072427877063E-4
80-81	0.0	0.0	0.0	0.27704494631207377	4.98126466946349E-4
82-83	0.0	7.21922415864274E-6	0.0	0.277485318985751	5.342225877395627E-4
84-85	0.0	1.443844831728548E-5	0.0	0.27781740329704857	5.342225877395627E-4
86-87	0.0	1.443844831728548E-5	0.0	0.2780339800218078	5.703187085327765E-4
88	0.0	1.443844831728548E-5	0.0	0.2780845145909183	6.064148293259901E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	11790	0.0	54.217525	1
GGTATCA	14080	0.0	46.292583	1
TATACAC	14950	0.0	43.935085	3
TTATACA	15580	0.0	41.136158	2
GTATCAA	25610	0.0	36.040546	1
ATACACA	19470	0.0	34.68398	4
ACACATC	20035	0.0	32.225502	6
ATCAACG	30315	0.0	30.059769	3
TACACAT	22345	0.0	29.435202	5
ACGCAGA	31640	0.0	28.794348	7
AACGCAG	31760	0.0	28.644917	6
TATCAAC	32030	0.0	28.554953	2
TCAACGC	31970	0.0	28.504684	4
CAACGCA	32105	0.0	28.347292	5
ACATCTC	24155	0.0	26.834064	8
CACATCT	25270	0.0	25.50073	7
CGCAGAG	36460	0.0	25.022242	8
CGCTACG	220	1.9970491E-4	23.215939	94
CATCTCC	28070	0.0	23.016762	9
GCAGAGT	41125	0.0	22.153269	9
>>END_MODULE