FastQCFastQC Report
Fri 27 May 2016
SRR2138766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365806
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC181400.043.674311
GGTATCA213400.042.0876771
TATACAC208700.039.0104643
TTATACA230700.034.7726442
GTATCAA373400.033.4571461
TACACAT286200.028.5867925
ATACACA305900.026.9532324
TATCAAC475200.026.1389662
ACACATC309000.026.0044826
ATCAACG479000.025.9232883
CAACGCA483500.025.6663175
TCAACGC484300.025.6478164
AACGCAG486650.025.5202056
CACATCT332250.024.2456917
ACGCAGA534300.023.2005167
CGCAGAG536000.023.1566498
CATCTCC360650.022.126649
GCAGAGT590500.020.8980339
TACATGG351650.018.9180362
GAGTACT399500.018.79184212-13
Produced by FastQC (version 0.11.5)