##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138766_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8365806
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66080303559514	33.0	33.0	33.0	33.0	33.0
2	32.60453995705853	33.0	33.0	33.0	33.0	33.0
3	32.67792678912229	33.0	33.0	33.0	33.0	33.0
4	36.64371669627529	37.0	37.0	37.0	37.0	37.0
5	36.64693156881716	37.0	37.0	37.0	37.0	37.0
6	36.636847543440524	37.0	37.0	37.0	37.0	37.0
7	36.61819255670046	37.0	37.0	37.0	37.0	37.0
8	36.615785854943326	37.0	37.0	37.0	37.0	37.0
9	36.61958704277866	37.0	37.0	37.0	37.0	37.0
10-11	36.620743835082955	37.0	37.0	37.0	37.0	37.0
12-13	36.618949626611	37.0	37.0	37.0	37.0	37.0
14-15	38.763729998042024	40.0	37.0	40.0	37.0	40.0
16-17	38.681961965171084	40.0	37.0	40.0	37.0	40.0
18-19	38.73746265452486	40.0	37.0	40.0	37.0	40.0
20-21	38.76504295032714	40.0	37.0	40.0	37.0	40.0
22-23	38.76749346558718	40.0	37.0	40.0	37.0	40.0
24-25	38.76146981434212	40.0	37.0	40.0	37.0	40.0
26-27	38.66924485742166	40.0	37.0	40.0	37.0	40.0
28-29	38.55805649130815	40.0	37.0	40.0	37.0	40.0
30-31	38.406876521888385	40.0	37.0	40.0	37.0	40.0
32-33	38.27730931425295	40.0	37.0	40.0	37.0	40.0
34-35	38.2230652474094	40.0	37.0	40.0	37.0	40.0
36-37	38.20039516107789	40.0	37.0	40.0	37.0	40.0
38-39	38.13915188486332	40.0	37.0	40.0	37.0	40.0
40-41	38.13775744854451	40.0	37.0	40.0	37.0	40.0
42-43	38.11916363056492	40.0	37.0	40.0	37.0	40.0
44-45	38.01537812681882	40.0	37.0	40.0	33.0	40.0
46-47	37.65019021167252	40.0	37.0	40.0	33.0	40.0
48-49	37.84474433585466	40.0	37.0	40.0	33.0	40.0
50-51	37.82573660274885	40.0	37.0	40.0	33.0	40.0
52-53	37.81272389734437	40.0	37.0	40.0	33.0	40.0
54-55	37.70094624153843	40.0	37.0	40.0	33.0	40.0
56-57	37.484835564704454	40.0	37.0	40.0	33.0	40.0
58-59	37.31544658933796	38.5	37.0	40.0	33.0	40.0
60-61	37.12446210769511	37.0	37.0	40.0	33.0	40.0
62-63	37.00085954399952	37.0	37.0	40.0	33.0	40.0
64-65	36.8627672949784	37.0	37.0	40.0	33.0	40.0
66-67	36.67399266462537	37.0	37.0	40.0	33.0	40.0
68-69	36.49821060705981	37.0	37.0	40.0	33.0	40.0
70-71	36.29734219866603	37.0	37.0	40.0	33.0	40.0
72-73	36.03271703527332	37.0	37.0	37.0	33.0	40.0
74-75	35.70803308795472	37.0	35.0	37.0	33.0	40.0
76-77	33.537488637656594	35.0	33.0	37.0	27.0	37.0
78-79	35.21290188087008	37.0	33.0	37.0	33.0	37.0
80-81	35.424103112695306	37.0	33.0	37.0	33.0	37.0
82-83	35.25611157945022	37.0	33.0	37.0	33.0	37.0
84-85	35.10679690636705	37.0	33.0	37.0	33.0	37.0
86-87	35.01206240710597	37.0	33.0	37.0	33.0	37.0
88-89	34.84113163775505	37.0	33.0	37.0	33.0	37.0
90-91	34.79074287132017	37.0	33.0	37.0	33.0	37.0
92-93	34.84438628362207	37.0	33.0	37.0	33.0	37.0
94-95	34.83386249854911	37.0	33.0	37.0	33.0	37.0
96-97	34.8420499148044	37.0	33.0	37.0	33.0	37.0
98-99	34.8524631323009	37.0	33.0	37.0	33.0	37.0
100	34.7011803309796	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	1.0
18	7.0
19	13.0
20	62.0
21	265.0
22	950.0
23	2725.0
24	6038.0
25	12178.0
26	21268.0
27	33977.0
28	50421.0
29	70597.0
30	95113.0
31	127246.0
32	169235.0
33	231837.0
34	328199.0
35	499823.0
36	957668.0
37	2576753.0
38	3059323.0
39	122104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64897520322312	21.01051841394821	14.816506002709842	23.524000380118828
2	14.183277942822361	20.69926042521217	43.39421687444035	21.723244757525116
3	16.988225641378726	27.646899772717653	32.3026376657551	23.062236920148518
4	11.536796334985535	18.963456718934193	38.57368913407746	30.92605781200281
5	12.135244350633997	38.16571887992622	36.05902407968819	13.64001268975159
6	27.625479242526062	38.809051990925916	18.864900763895314	14.700568002652703
7	24.236266057329082	34.20788146414105	23.0881399831648	18.46771249536506
8	23.72696665449808	36.398812021220664	21.895905786005557	17.97831553827569
9	24.639849406022563	16.534330344260912	21.584734334025914	37.24108591569061
10-11	23.217478716442546	27.020305542717043	29.046634212208787	20.71558152863162
12-13	24.74521088026319	24.590645249792143	29.070334863309327	21.593809006635343
14-15	21.962014180104106	25.985697014728764	27.18579656281774	24.86649224234939
16-17	20.691855632320426	29.55640496564228	27.758227957951693	21.993511444085602
18-19	20.44418672869058	27.263822517519532	30.683271880796664	21.60871887299323
20-21	21.272235987193497	26.322014020307606	30.879851225943128	21.52589876655577
22-23	20.73135564780222	27.079330950903298	30.8272884879994	21.36202491329508
24-25	20.529702923824434	27.55713482868266	31.220350635546286	20.692811611946617
26-27	20.387081097484703	27.48841928964066	31.275637745892503	20.848861866982134
28-29	19.53940502176511	26.804816383851858	31.503770242156033	22.152008352227
30-31	19.967274189990903	26.792593413941233	31.7416956652072	21.49843673086066
32-33	19.843145808003335	27.450339145827286	31.545249367678903	21.16126567849048
34-35	19.983883012697806	28.217606345704834	31.59545574376813	20.20305489782923
36-37	20.39941312936266	27.539941129813876	30.60804691058488	21.452598830238585
38-39	20.534155818347067	27.16790605595949	31.00701950757142	21.290918618122017
40-41	20.607599012356772	27.169473662535264	31.15713623398933	21.065791091118633
42-43	21.128418215523183	27.145586886097483	30.87419475121065	20.851800147168685
44-45	20.587904841870724	28.045209771010988	30.136937074974167	21.229948312144124
46-47	21.57599684385184	27.243880317516016	28.960547691590705	22.21957514704144
48-49	21.39007226020325	27.363777621140866	29.122424818865845	22.12372529979004
50-51	20.756412366286586	27.95119313506524	29.270462980066835	22.021931518581336
52-53	20.921959039432924	28.366915945385458	29.487817222761898	21.223307792419718
54-55	20.98304449213187	28.461173527966267	28.608360051482617	21.947421928419246
56-57	21.761503722928683	28.83074341779493	27.767392580224108	21.640360279052278
58-59	20.75915564282461	29.422037355969	29.003052265173174	20.81575473603322
60-61	21.552264220539225	29.994674136339512	27.473926420856614	20.97913522226465
62-63	20.895072015096588	30.390005492229317	27.446389152612387	21.268533340061705
64-65	20.914342781470335	30.05980321963072	27.638059409548294	21.387794589350655
66-67	21.27516746734612	29.532802697934773	27.563523914498983	21.628505920220118
68-69	21.386152667320474	28.967016512216	27.921410508624607	21.72542031183892
70-71	21.599878420434997	28.206237335791958	27.981570147180012	22.212314096593033
72-73	21.673157952957432	28.13466237784268	28.11212944699503	22.080050222204857
74-75	21.55043343487649	28.27226301308572	28.087830250322277	22.089473301715515
76-77	21.62406615018512	28.23994128198313	27.852699551930005	22.283293015901755
78-79	21.812495551321913	28.307619800136802	27.76592622583455	22.113958422706737
80-81	21.659293276668283	28.17866533954391	27.90286030100762	22.259181082780184
82-83	22.07455934254107	27.83052863091431	28.04118944038536	22.053722586159257
84-85	22.288762689044926	27.865246044471327	27.726039215405706	22.119952051078045
86-87	21.761536077963186	28.02122156963985	28.002517939610243	22.214724412786722
88-89	22.022358367034315	27.719136655971464	27.962471004889057	22.296033972105164
90-91	22.11844095690589	27.669010000730836	28.030627995656104	22.18192104670717
92-93	22.171414045602596	27.67695327266779	27.96623659337208	22.185396088357532
94-95	21.804455173927355	27.70068559487402	28.172538991121144	22.32232024007748
96-97	21.975248688605	27.808710896372457	28.215563579616283	22.00047683540626
98-99	23.098507158787104	29.728049868129002	28.351564794950406	18.821878178133485
100	29.70316142288943	40.73509847429071	29.56174010281986	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2760.0
1	2698.5
2	4777.5
3	7240.0
4	10510.0
5	19312.5
6	26170.0
7	29018.0
8	29223.0
9	26330.0
10	22691.0
11	19468.5
12	21361.5
13	30101.5
14	50166.5
15	78365.0
16	101270.0
17	113855.0
18	115772.5
19	108076.5
20	94578.5
21	79075.0
22	67385.5
23	64663.5
24	70097.0
25	82199.5
26	100330.0
27	117683.5
28	131318.5
29	147545.5
30	162784.5
31	174228.0
32	190286.0
33	210711.5
34	230228.5
35	249938.0
36	271935.5
37	289475.5
38	296857.0
39	299395.5
40	309369.5
41	323209.0
42	332066.5
43	340562.5
44	359576.0
45	394534.0
46	420564.5
47	434329.5
48	429454.5
49	409186.5
50	392932.5
51	366371.0
52	330621.0
53	297267.5
54	266643.5
55	236330.0
56	203254.0
57	167358.0
58	137977.5
59	112298.0
60	86102.0
61	64179.0
62	47362.0
63	34733.5
64	24510.0
65	16723.5
66	11893.0
67	8909.5
68	6567.5
69	4600.5
70	3312.0
71	2306.0
72	1656.5
73	1186.5
74	799.5
75	518.5
76	329.0
77	226.0
78	150.5
79	97.0
80	75.0
81	65.5
82	60.5
83	47.5
84	32.0
85	19.0
86	13.0
87	11.0
88	6.5
89	2.0
90	0.5
91	0.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.195342086584365E-5
2	6.335313058897134E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0016077351064559709
12-13	0.008977019070248582
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.97779404933318E-6
24-25	5.97927630666321E-6
26-27	1.1964005809960502E-5
28-29	5.986744151100634E-6
30-31	6.0001114820713366E-6
32-33	0.0
34-35	0.006063341383261992
36-37	0.04814410309171046
38-39	0.1542583396280625
40-41	0.18252499869980912
42-43	0.05701253916379885
44-45	0.005047337363004503
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.3592941294329386E-5
54-55	2.7510206630537512E-5
56-57	6.981123461028787E-6
58-59	0.0
60-61	0.0
62-63	0.015319857578859682
64-65	0.10749380361673352
66-67	0.23810761378856635
68-69	0.26593520872491494
70-71	0.121139618888352
72-73	0.00819749645891414
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	218.0
20-21	882.0
22-23	1899.0
24-25	3169.0
26-27	5711.0
28-29	12974.0
30-31	32638.0
32-33	41090.0
34-35	55024.0
36-37	74532.0
38-39	87168.0
40-41	95611.0
42-43	115232.0
44-45	137586.0
46-47	127123.0
48-49	107580.0
50-51	90015.0
52-53	83140.0
54-55	102405.0
56-57	114320.0
58-59	115705.0
60-61	115639.0
62-63	117613.0
64-65	129139.0
66-67	139128.0
68-69	114515.0
70-71	91911.0
72-73	82824.0
74-75	81424.0
76-77	76942.0
78-79	82254.0
80-81	87060.0
82-83	90431.0
84-85	92493.0
86-87	92544.0
88-89	97226.0
90-91	99403.0
92-93	103636.0
94-95	108900.0
96-97	236316.0
98-99	1570699.0
100-101	3351687.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.07678086238704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.16210826996809	68.05336826897951
2	7.757600904898906	12.113770117392948
3	2.344993840850022	5.492687107070834
4	1.0733539411601953	3.3521608180697617
5	0.5674543481650816	2.2152504395546875
6	0.33030201194610925	1.5473350683073153
7	0.20054004375325432	1.0960264735179444
8	0.14604188533616455	0.912198422249726
9	0.09180231214005966	0.6450866106857903
>10	0.3200106726320193	3.6354378211952816
>50	0.003812469200277914	0.19280699764966494
>100	0.0013424246842226753	0.19739225496721902
>500	3.016777862333884E-4	0.15671281028599618
>1k	3.351974793120036E-4	0.3897667900732628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.39068417316873E-5	0.0
6	0.0	0.0	0.0	2.39068417316873E-5	0.0
7	0.0	0.0	0.0	2.39068417316873E-5	0.0
8	0.0	0.0	0.0	2.39068417316873E-5	0.0
9	0.0	0.0	0.0	3.586026259753095E-5	0.0
10-11	0.0	0.0	0.0	3.586026259753095E-5	0.0
12-13	0.0	0.0	0.0	3.586026259753095E-5	0.0
14-15	0.0	0.0	0.0	5.3790393896296424E-5	0.0
16-17	0.0	0.0	0.0	9.56273669267492E-5	0.0
18-19	0.0	0.0	0.0	1.6137118168888927E-4	0.0
20-21	0.0	0.0	0.0	3.167656529448567E-4	0.0
22-23	0.0	0.0	0.0	0.0010519010361942412	0.0
24-25	0.0	0.0	0.0	0.0037055604684115315	0.0
26-27	0.0	0.0	0.0	0.008905298545053519	0.0
28-29	0.0	0.0	0.0	0.03459319998575152	0.0
30-31	0.0	0.0	0.0	0.04902695568125773	0.0
32-33	0.0	0.0	0.0	0.055810522022624	0.0
34-35	0.0	0.0	0.0	0.06407631255135489	0.0
36-37	0.0	0.0	0.0	0.07654970722486273	0.0
38-39	0.0	0.0	0.0	0.10785571647250726	0.0
40-41	0.0	0.0	0.0	0.14091290187699787	0.0
42-43	0.0	0.0	0.0	0.1612098105072004	0.0
44-45	0.0	0.0	0.0	0.1863239477463379	0.0
46-47	0.0	0.0	0.0	0.2108344372317503	0.0
48-49	0.0	0.0	0.0	0.23367742450637752	0.0
50-51	0.0	0.0	0.0	0.2558749270542492	0.0
52-53	0.0	0.0	0.0	0.30273233684835626	0.0
54-55	5.976710432921825E-6	0.0	0.0	0.3564390567985918	0.0
56-57	1.195342086584365E-5	0.0	0.0	0.4139350111632998	0.0
58-59	1.195342086584365E-5	0.0	0.0	0.4592205461135484	0.0
60-61	1.195342086584365E-5	0.0	0.0	0.4780352305563863	0.0
62-63	1.195342086584365E-5	0.0	0.0	0.5019002353150432	0.0
64-65	1.195342086584365E-5	0.0	0.0	0.5108832310957248	0.0
66-67	1.195342086584365E-5	0.0	0.0	0.5180373534839321	0.0
68-69	1.195342086584365E-5	0.0	0.0	0.5247850595627008	0.0
70-71	1.195342086584365E-5	0.0	0.0	0.5307438398643238	0.0
72-73	1.195342086584365E-5	0.0	0.0	0.5356387657088868	0.0
74-75	1.195342086584365E-5	0.0	0.0	0.5393861631503288	0.0
76-77	1.195342086584365E-5	0.0	0.0	0.5428705853327223	0.0
78-79	1.195342086584365E-5	0.0	0.0	0.5450879449033362	0.0
80-81	1.195342086584365E-5	0.0	0.0	0.545799173444854	0.0
82-83	1.195342086584365E-5	0.0	0.0	0.5464028211985791	0.0
84-85	1.195342086584365E-5	0.0	0.0	0.5466538430367618	0.0
86-87	1.195342086584365E-5	0.0	0.0	0.5468331443497494	0.0
88	1.195342086584365E-5	0.0	0.0	0.5468570511914811	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	18140	0.0	43.67431	1
GGTATCA	21340	0.0	42.087677	1
TATACAC	20870	0.0	39.010464	3
TTATACA	23070	0.0	34.772644	2
GTATCAA	37340	0.0	33.457146	1
TACACAT	28620	0.0	28.586792	5
ATACACA	30590	0.0	26.953232	4
TATCAAC	47520	0.0	26.138966	2
ACACATC	30900	0.0	26.004482	6
ATCAACG	47900	0.0	25.923288	3
CAACGCA	48350	0.0	25.666317	5
TCAACGC	48430	0.0	25.647816	4
AACGCAG	48665	0.0	25.520205	6
CACATCT	33225	0.0	24.245691	7
ACGCAGA	53430	0.0	23.200516	7
CGCAGAG	53600	0.0	23.156649	8
CATCTCC	36065	0.0	22.12664	9
GCAGAGT	59050	0.0	20.898033	9
TACATGG	35165	0.0	18.918036	2
GAGTACT	39950	0.0	18.791842	12-13
>>END_MODULE