FastQCFastQC Report
Fri 27 May 2016
SRR2138765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714296
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178500.047.743341
GTATCAA325100.035.473541
ATCAACG402750.028.2954543
TCAACGC405450.028.1478234
TATCAAC407900.028.0193042
CAACGCA408850.027.9141065
AACGCAG410350.027.9063786
CGCAGAG461050.024.8294498
ACGCAGA460800.024.824977
GCAGAGT508100.022.4488499
GTGGTAT80850.022.2475511
GTACATG297750.020.914441
GAGTACT355200.020.6186812-13
TACATGG293800.020.5904352
TGGTATC84100.020.5519642
ACATGGG294200.020.1689013
CAGAGTA507750.019.57686810-11
AGAGTAC493300.018.77119810-11
GTACTTT392250.018.62255114-15
AGTACTT380250.018.17826312-13
Produced by FastQC (version 0.11.5)