##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7714296
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69713970011003	33.0	33.0	33.0	33.0	33.0
2	32.63298828564525	33.0	33.0	33.0	33.0	33.0
3	32.70596448463995	33.0	33.0	33.0	33.0	33.0
4	36.67873996538375	37.0	37.0	37.0	37.0	37.0
5	36.682684849012794	37.0	37.0	37.0	37.0	37.0
6	36.67690583819962	37.0	37.0	37.0	37.0	37.0
7	36.66170808068552	37.0	37.0	37.0	37.0	37.0
8	36.65455577540711	37.0	37.0	37.0	37.0	37.0
9	36.652004408438565	37.0	37.0	37.0	37.0	37.0
10-11	36.65627349533904	37.0	37.0	37.0	37.0	37.0
12-13	36.6561537825357	37.0	37.0	37.0	37.0	37.0
14-15	38.85840768878975	40.0	37.0	40.0	37.0	40.0
16-17	38.79355076600639	40.0	37.0	40.0	37.0	40.0
18-19	38.84112646183139	40.0	37.0	40.0	37.0	40.0
20-21	38.85763901253835	40.0	37.0	40.0	37.0	40.0
22-23	38.86184829189651	40.0	37.0	40.0	37.0	40.0
24-25	38.85681449484528	40.0	37.0	40.0	37.0	40.0
26-27	38.77584675226139	40.0	37.0	40.0	37.0	40.0
28-29	38.672508291533475	40.0	37.0	40.0	37.0	40.0
30-31	38.51723285946706	40.0	37.0	40.0	37.0	40.0
32-33	38.408556409404056	40.0	37.0	40.0	37.0	40.0
34-35	38.37708108935111	40.0	37.0	40.0	37.0	40.0
36-37	38.34975352952617	40.0	37.0	40.0	37.0	40.0
38-39	38.3046046182083	40.0	37.0	40.0	37.0	40.0
40-41	38.278314672438874	40.0	37.0	40.0	37.0	40.0
42-43	38.24886889201264	40.0	37.0	40.0	37.0	40.0
44-45	38.162975385815564	40.0	37.0	40.0	37.0	40.0
46-47	37.800458286449654	40.0	37.0	40.0	33.0	40.0
48-49	37.972541371207605	40.0	37.0	40.0	33.0	40.0
50-51	37.98913819964561	40.0	37.0	40.0	33.0	40.0
52-53	37.95810585536668	40.0	37.0	40.0	33.0	40.0
54-55	37.86951040240341	40.0	37.0	40.0	33.0	40.0
56-57	37.709005052101126	40.0	37.0	40.0	33.0	40.0
58-59	37.54798256055193	40.0	37.0	40.0	33.0	40.0
60-61	37.37494931684542	38.5	37.0	40.0	33.0	40.0
62-63	37.24173200012561	37.0	37.0	40.0	33.0	40.0
64-65	37.065114059083015	37.0	37.0	40.0	33.0	40.0
66-67	36.86008186043168	37.0	37.0	40.0	33.0	40.0
68-69	36.65682069813106	37.0	37.0	40.0	33.0	40.0
70-71	36.43002524709574	37.0	37.0	40.0	33.0	40.0
72-73	36.14828848210553	37.0	37.0	37.0	33.0	40.0
74-75	35.81006297127974	37.0	37.0	37.0	33.0	40.0
76-77	33.655319010168746	35.0	33.0	37.0	30.0	37.0
78-79	35.30946484156375	37.0	33.0	37.0	33.0	37.0
80-81	35.50093291581881	37.0	33.0	37.0	33.0	37.0
82-83	35.32556579949383	37.0	33.0	37.0	33.0	37.0
84-85	35.17240628632557	37.0	33.0	37.0	33.0	37.0
86-87	35.07161332621099	37.0	33.0	37.0	33.0	37.0
88-89	34.895680050698516	37.0	33.0	37.0	33.0	37.0
90-91	34.8372772709873	37.0	33.0	37.0	33.0	37.0
92-93	34.87603896892891	37.0	33.0	37.0	33.0	37.0
94-95	34.855942051600124	37.0	33.0	37.0	33.0	37.0
96-97	34.8519408746357	37.0	33.0	37.0	33.0	37.0
98-99	34.85833021401159	37.0	33.0	37.0	33.0	37.0
100	34.809963378200045	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	5.0
19	11.0
20	44.0
21	186.0
22	617.0
23	1908.0
24	4420.0
25	9138.0
26	16347.0
27	27133.0
28	40753.0
29	58876.0
30	81511.0
31	111344.0
32	149275.0
33	205167.0
34	291529.0
35	446482.0
36	859217.0
37	2366113.0
38	2938663.0
39	105552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82932359072652	20.504715013454245	15.021167199487081	22.64479419633216
2	14.602994972946206	20.988727369831974	42.77313702156785	21.635140635653972
3	17.692053219646542	27.05279316551845	31.24766828954147	24.00748532529354
4	11.941416803663433	18.30636737464245	39.72399807422429	30.028217747469828
5	12.537038386143957	38.13932940590546	35.92061697415214	13.40301523379845
6	28.477680523972772	38.71610286048212	19.07077951657015	13.735437098974968
7	25.204522928475377	33.8518340109931	23.749807824280484	17.193835236251044
8	23.724669590675493	36.844416761350196	21.460833426774578	17.970080221199733
9	25.394993917785886	17.140125294647753	22.091711285125694	35.37316950244067
10-11	23.565753621147177	27.254902329344933	29.171188921366664	20.008155128141226
12-13	24.737604225997856	25.048795394982605	29.485868862687937	20.727731516331605
14-15	21.725812698916403	26.286915876704757	28.046966307748626	23.940305116630213
16-17	20.39366910473749	29.29217390673109	28.793521793822798	21.52063519470863
18-19	20.203580469300114	27.853060084808778	31.583893073327758	20.359466372563357
20-21	21.201099397838306	27.074745908143345	31.636959150204152	20.087195543814197
22-23	20.895322014429155	26.950148138856722	31.50565540059398	20.648874446120143
24-25	20.46961088824085	27.244501467416505	31.644248235624868	20.641639408717772
26-27	20.454524962969952	27.46011992967291	31.51058263092193	20.574772476435204
28-29	20.37992957842486	27.564947926492433	31.488200120983116	20.56692237409959
30-31	20.68178679871031	27.45696725483272	31.30501759632972	20.556228350127252
32-33	20.388993768093403	27.686454519533775	31.167795117888442	20.75675659448438
34-35	20.58560358573102	27.83850051248327	31.068764268968636	20.507131632817075
36-37	20.726713284056064	27.662772853759765	30.981032653590596	20.629481208593575
38-39	20.56885573536425	27.93355331675169	30.85194540720201	20.645645540682054
40-41	20.820584583028413	27.866638287697025	30.705962265155236	20.60681486411933
42-43	20.810620759819464	28.018565699922966	30.240639178086447	20.930174362171122
44-45	20.89028935983501	28.2888317973434	29.799507050401502	21.021371792420084
46-47	21.11800052010849	28.254015859128085	29.210384163950682	21.417599456812738
48-49	21.129610215042373	28.49313547593158	29.01273857459387	21.364515734432178
50-51	21.150668549764575	28.567459696632643	28.81832748615716	21.463544267445624
52-53	21.352718001752883	28.607024552640127	28.590709427256115	21.44954801835087
54-55	21.357729065906454	28.56370037045613	28.587860235906952	21.49071032773047
56-57	21.39691216855787	28.602289027130706	28.447446148993865	21.55335265531756
58-59	21.412481344163524	28.496982705327277	28.581098563372265	21.50943738713693
60-61	21.395940190675418	28.605867198457414	28.53315830090992	21.46503430995724
62-63	21.3944424375798	28.574953509322963	28.502205298760398	21.528398754336838
64-65	21.406195633038443	28.479896109652756	28.45502400415267	21.65888425315613
66-67	21.525156663362168	28.566192417093873	28.172099927004655	21.736550992539307
68-69	21.536396172002025	28.45670858846506	28.353099162507505	21.653796077025408
70-71	21.498210955896564	28.173864475848482	28.344163015757516	21.983761552497437
72-73	21.6305400434624	28.229321323805166	28.32746171352831	21.81267691920411
74-75	21.564648716737164	28.338248473933465	28.238097630756393	21.85900517857298
76-77	21.60386891753915	28.35062705291286	28.148841916293264	21.896662113254727
78-79	21.700427707238134	28.172904606658104	28.227101267689108	21.89956641841465
80-81	21.653323705185556	28.159023974507804	28.2286825041301	21.95896981617655
82-83	21.864198293470032	28.11954325432272	28.173378401009476	21.84288005119777
84-85	22.017267102349443	28.06531147545496	28.079477387093725	21.837944035101874
86-87	21.78101665390113	28.16354590383258	28.15276095202266	21.902676490243632
88-89	21.98261657531313	27.961854515141916	28.075744087387903	21.979784822157054
90-91	21.991882850280735	27.891346941497865	28.123835657516366	21.99293455070504
92-93	22.05853424388063	27.94499090311161	28.082078938086912	21.91439591492085
94-95	21.913607416858422	27.868488937670975	28.196458717538707	22.021444927931892
96-97	22.04125444936966	27.96447780924256	28.2014237982912	21.792843943096578
98-99	23.08899329668029	29.828514976846037	28.409101992946262	18.673389733527408
100	22.0454921887637	30.7220781659937	22.231778107603002	25.0006515376396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2378.0
1	2343.5
2	4025.0
3	6042.0
4	8593.5
5	15100.5
6	20365.5
7	23352.0
8	24772.5
9	23470.0
10	20541.0
11	17886.0
12	19689.0
13	26890.0
14	43329.5
15	66179.5
16	84651.0
17	94688.0
18	95891.0
19	89343.0
20	78691.0
21	67071.5
22	58703.0
23	56646.5
24	60292.0
25	69261.0
26	83449.5
27	98799.0
28	112600.0
29	128085.0
30	145164.5
31	162325.5
32	180939.5
33	199513.5
34	216980.0
35	234828.5
36	256073.5
37	276427.5
38	289604.5
39	298447.0
40	308894.0
41	317392.5
42	321762.5
43	327566.5
44	333520.5
45	340411.5
46	342342.5
47	337712.0
48	333551.5
49	325080.0
50	309929.5
51	293603.0
52	274633.0
53	251682.5
54	226826.5
55	199888.0
56	172085.5
57	144580.5
58	121413.0
59	101869.5
60	81233.5
61	62379.0
62	47269.5
63	34383.5
64	24515.0
65	17572.0
66	12771.0
67	9816.0
68	7446.0
69	5453.0
70	3952.5
71	2712.5
72	1924.0
73	1454.0
74	1106.0
75	764.0
76	468.0
77	293.5
78	204.5
79	139.0
80	94.0
81	62.0
82	45.0
83	29.0
84	20.0
85	15.5
86	11.0
87	8.0
88	5.0
89	3.0
90	2.5
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5925891358070782E-5
2	4.666660444452741E-4
3	2.5925891358070782E-5
4	2.5925891358070782E-5
5	2.5925891358070782E-5
6	2.5925891358070782E-5
7	2.5925891358070782E-5
8	1.2962945679035391E-5
9	0.0
10-11	0.0017046273567931538
12-13	0.00919720995927561
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.486541464308195E-6
28-29	2.5967608265126165E-5
30-31	2.6017077479572205E-5
32-33	2.6104679896910008E-5
34-35	0.005846870718084869
36-37	0.04855616617813783
38-39	0.1557268349104107
40-41	0.1843892654817687
42-43	0.05758366966168171
44-45	0.005177110388283966
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.015037834843278206
64-65	0.10707088718276313
66-67	0.22964684839121616
68-69	0.2535907051038737
70-71	0.11675562635660884
72-73	0.007970361492414473
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	140.0
20-21	672.0
22-23	1447.0
24-25	2755.0
26-27	5148.0
28-29	11046.0
30-31	24312.0
32-33	31469.0
34-35	41156.0
36-37	57461.0
38-39	67585.0
40-41	73152.0
42-43	89772.0
44-45	107030.0
46-47	99808.0
48-49	83917.0
50-51	71785.0
52-53	65761.0
54-55	70004.0
56-57	71451.0
58-59	68385.0
60-61	67397.0
62-63	63550.0
64-65	69370.0
66-67	75937.0
68-69	72018.0
70-71	75093.0
72-73	69980.0
74-75	67873.0
76-77	64813.0
78-79	71361.0
80-81	70815.0
82-83	70983.0
84-85	75746.0
86-87	82796.0
88-89	87820.0
90-91	87282.0
92-93	90501.0
94-95	93858.0
96-97	232463.0
98-99	437521.0
100-101	4642863.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.42914498408791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3675477808127	69.39529619586399
2	7.837921103952859	12.45118743479428
3	2.358792396868279	5.620705898346442
4	0.999370008337778	3.1751642114004204
5	0.5063513167545722	2.010952607569137
6	0.29730016150026806	1.4168578578958524
7	0.17944436155732432	0.9977178547499663
8	0.11246915991169826	0.7146663367091878
9	0.07991466746956488	0.5712798337915718
>10	0.25702855346618114	3.051610967102205
>50	0.0026715193244852137	0.13423570436797347
>100	8.193740208546176E-4	0.15816463254841961
>500	2.015978336894014E-4	0.1123027076987618
>1k	1.679981897372771E-4	0.18985775716178382
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.2962945679035391E-5	0.0
12-13	0.0	0.0	0.0	1.2962945679035391E-5	0.0
14-15	0.0	0.0	0.0	1.2962945679035391E-5	0.0
16-17	6.4814728395176955E-6	0.0	0.0	1.2962945679035391E-5	0.0
18-19	1.2962945679035391E-5	0.0	0.0	1.2962945679035391E-5	0.0
20-21	2.5925891358070782E-5	0.0	0.0	1.2962945679035391E-5	0.0
22-23	3.888883703710617E-5	0.0	0.0	2.5925891358070782E-5	0.0
24-25	3.888883703710617E-5	0.0	0.0	5.1851782716141564E-5	0.0
26-27	4.537030987662387E-5	0.0	0.0	1.2962945679035392E-4	0.0
28-29	5.1851782716141564E-5	0.0	0.0	6.092584469146634E-4	0.0
30-31	6.481472839517694E-5	0.0	0.0	0.0015361090629656938	0.0
32-33	7.777767407421234E-5	0.0	0.0	0.0038370319209944754	0.0
34-35	7.777767407421234E-5	0.0	0.0	0.0064620284209991425	0.0
36-37	7.777767407421234E-5	0.0	0.0	0.010111097629647604	0.0
38-39	9.074061975324773E-5	0.0	0.0	0.015458312722249703	0.0
40-41	1.0370356543228313E-4	0.0	0.0	0.020922194325963123	0.0
42-43	1.0370356543228313E-4	0.0	0.0	0.026476816549429787	0.0
44-45	1.0370356543228313E-4	0.0	0.0	0.03251106776302076	0.0
46-47	1.0370356543228313E-4	0.0	0.0	0.03918050331488447	0.0
48-49	1.0370356543228313E-4	0.0	0.0	0.046024938633415155	0.0
50-51	1.0370356543228313E-4	0.0	0.0	0.05246104116305623	0.0
52-53	1.0370356543228313E-4	0.0	0.0	0.059169365551957044	6.4814728395176955E-6
54-55	1.0370356543228313E-4	0.0	0.0	0.06471102482974467	1.2962945679035391E-5
56-57	1.1018503827180082E-4	0.0	0.0	0.07060916511370577	1.2962945679035391E-5
58-59	1.2962945679035392E-4	0.0	0.0	0.07673415694704999	1.2962945679035391E-5
60-61	1.2962945679035392E-4	0.0	0.0	0.08258692692113448	1.2962945679035391E-5
62-63	1.425924024693893E-4	0.0	0.0	0.08810914178040355	1.2962945679035391E-5
64-65	2.3981449506215474E-4	0.0	0.0	0.09408505973843886	1.2962945679035391E-5
66-67	2.5925891358070783E-4	0.0	0.0	0.100184125680425	1.2962945679035391E-5
68-69	2.5925891358070783E-4	0.0	0.0	0.10662022821006609	1.2962945679035391E-5
70-71	2.5925891358070783E-4	0.0	0.0	0.11198040624834722	1.2962945679035391E-5
72-73	2.5925891358070783E-4	0.0	0.0	0.11597299351749013	1.2962945679035391E-5
74-75	2.916662777782963E-4	0.0	0.0	0.11912298931749572	1.2962945679035391E-5
76-77	3.1111069629684936E-4	0.0	0.0	0.12241557751997072	1.2962945679035391E-5
78-79	3.1111069629684936E-4	0.0	0.0	0.12415261224096147	1.2962945679035391E-5
80-81	3.1111069629684936E-4	0.0	0.0	0.12471001890515998	1.2962945679035391E-5
82-83	3.1111069629684936E-4	0.0	0.0	0.12522205525948188	1.2962945679035391E-5
84-85	3.1111069629684936E-4	0.0	0.0	0.12548131417306257	1.2962945679035391E-5
86-87	3.1111069629684936E-4	0.0	0.0	0.12562390657553196	1.2962945679035391E-5
88	3.1111069629684936E-4	0.0	0.0	0.12566279541256908	1.2962945679035391E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17850	0.0	47.74334	1
GTATCAA	32510	0.0	35.47354	1
ATCAACG	40275	0.0	28.295454	3
TCAACGC	40545	0.0	28.147823	4
TATCAAC	40790	0.0	28.019304	2
CAACGCA	40885	0.0	27.914106	5
AACGCAG	41035	0.0	27.906378	6
CGCAGAG	46105	0.0	24.829449	8
ACGCAGA	46080	0.0	24.82497	7
GCAGAGT	50810	0.0	22.448849	9
GTGGTAT	8085	0.0	22.247551	1
GTACATG	29775	0.0	20.91444	1
GAGTACT	35520	0.0	20.61868	12-13
TACATGG	29380	0.0	20.590435	2
TGGTATC	8410	0.0	20.551964	2
ACATGGG	29420	0.0	20.168901	3
CAGAGTA	50775	0.0	19.576868	10-11
AGAGTAC	49330	0.0	18.771198	10-11
GTACTTT	39225	0.0	18.622551	14-15
AGTACTT	38025	0.0	18.178263	12-13
>>END_MODULE