FastQCFastQC Report
Fri 27 May 2016
SRR2138764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110658
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC150950.048.537651
TATACAC174250.044.239763
GGTATCA319300.041.9041371
TTATACA200250.037.3631482
GTATCAA560950.033.45861
TACACAT257450.029.6190725
ATACACA279850.027.9585194
ACACATC267250.027.8834926
TATCAAC702750.026.5034922
ATCAACG704500.026.409283
AACGCAG705550.026.3875246
ACATCTC283900.026.2636498
TCAACGC714950.026.0232724
CAACGCA714800.025.9849645
CACATCT287600.025.9257687
ACGCAGA782350.023.7679397
CGCAGAG787550.023.5907828
CATCTCC322550.023.0054919
TGCGCGA6257.2759576E-1222.30238294
GCAGAGT851250.021.7740159
Produced by FastQC (version 0.11.5)