##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10110658
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.705932788944104	33.0	33.0	33.0	33.0	33.0
2	32.64251495797801	33.0	33.0	33.0	33.0	33.0
3	32.71098260864921	33.0	33.0	33.0	33.0	33.0
4	36.689200940235544	37.0	37.0	37.0	37.0	37.0
5	36.69446676962073	37.0	37.0	37.0	37.0	37.0
6	36.685941607361265	37.0	37.0	37.0	37.0	37.0
7	36.67070501247298	37.0	37.0	37.0	37.0	37.0
8	36.667098026656625	37.0	37.0	37.0	37.0	37.0
9	36.66339312436441	37.0	37.0	37.0	37.0	37.0
10-11	36.66959103947538	37.0	37.0	37.0	37.0	37.0
12-13	36.66791711281303	37.0	37.0	37.0	37.0	37.0
14-15	38.847940064830595	40.0	37.0	40.0	37.0	40.0
16-17	38.7773845678491	40.0	37.0	40.0	37.0	40.0
18-19	38.84355869815792	40.0	37.0	40.0	37.0	40.0
20-21	38.86694665311276	40.0	37.0	40.0	37.0	40.0
22-23	38.86786663725976	40.0	37.0	40.0	37.0	40.0
24-25	38.860005802817795	40.0	37.0	40.0	37.0	40.0
26-27	38.7702458261948	40.0	37.0	40.0	37.0	40.0
28-29	38.65333159501702	40.0	37.0	40.0	37.0	40.0
30-31	38.4677782934468	40.0	37.0	40.0	37.0	40.0
32-33	38.35980982486588	40.0	37.0	40.0	37.0	40.0
34-35	38.32516108128179	40.0	37.0	40.0	37.0	40.0
36-37	38.28684869368435	40.0	37.0	40.0	37.0	40.0
38-39	38.221433243232276	40.0	37.0	40.0	37.0	40.0
40-41	38.20881165215229	40.0	37.0	40.0	37.0	40.0
42-43	38.18704428736447	40.0	37.0	40.0	37.0	40.0
44-45	38.08992017637969	40.0	37.0	40.0	35.0	40.0
46-47	37.718368948512136	40.0	37.0	40.0	33.0	40.0
48-49	37.892964981043406	40.0	37.0	40.0	33.0	40.0
50-51	37.87132811553968	40.0	37.0	40.0	33.0	40.0
52-53	37.85048781693255	40.0	37.0	40.0	33.0	40.0
54-55	37.76879145480906	40.0	37.0	40.0	33.0	40.0
56-57	37.59108572143727	40.0	37.0	40.0	33.0	40.0
58-59	37.43368353434731	40.0	37.0	40.0	33.0	40.0
60-61	37.23681309031541	37.0	37.0	40.0	33.0	40.0
62-63	37.10863597594299	37.0	37.0	40.0	33.0	40.0
64-65	36.96767186586964	37.0	37.0	40.0	33.0	40.0
66-67	36.79396932808271	37.0	37.0	40.0	33.0	40.0
68-69	36.618994830692216	37.0	37.0	40.0	33.0	40.0
70-71	36.41481582575888	37.0	37.0	40.0	33.0	40.0
72-73	36.1436967917257	37.0	37.0	37.0	33.0	40.0
74-75	35.81968167232522	37.0	37.0	37.0	33.0	40.0
76-77	33.64109610802843	35.0	33.0	37.0	30.0	37.0
78-79	35.3147580673529	37.0	33.0	37.0	33.0	37.0
80-81	35.51725321851659	37.0	33.0	37.0	33.0	37.0
82-83	35.347335297105545	37.0	33.0	37.0	33.0	37.0
84-85	35.19995719717941	37.0	33.0	37.0	33.0	37.0
86-87	35.111175138632	37.0	33.0	37.0	33.0	37.0
88-89	34.94577239885243	37.0	33.0	37.0	33.0	37.0
90-91	34.8880775310595	37.0	33.0	37.0	33.0	37.0
92-93	34.935997327991146	37.0	33.0	37.0	33.0	37.0
94-95	34.92225937854402	37.0	33.0	37.0	33.0	37.0
96-97	34.92144867631636	37.0	33.0	37.0	33.0	37.0
98-99	34.932930083254824	37.0	33.0	37.0	33.0	37.0
100	34.78136847471078	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	5.0
19	22.0
20	95.0
21	236.0
22	880.0
23	2480.0
24	5888.0
25	11795.0
26	21135.0
27	34374.0
28	52054.0
29	73866.0
30	102207.0
31	138865.0
32	188944.0
33	261506.0
34	375944.0
35	585519.0
36	1151214.0
37	3139347.0
38	3801829.0
39	162452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42360877240717	20.897652842935923	14.959126790672455	22.719611593984446
2	14.354507518770593	20.900044893349364	43.241701381783464	21.503746206096576
3	17.22811880573142	27.974126706108223	32.125597772726344	22.67215671543402
4	11.593994336866537	19.00569864055323	38.74178502940017	30.658521993180067
5	12.008527240119014	38.455809548281586	35.90132668925472	13.63433652234469
6	27.672425243977717	38.75780772703495	18.899751025081756	14.67001600390558
7	24.048031695665276	34.593478940092616	23.21407995543712	18.144409408804986
8	23.879773589391867	36.27135209907724	21.748517430667462	18.10035688086343
9	25.132854854748327	16.5321980033347	21.665731350026874	36.6692157918901
10-11	23.348907764679655	27.20212682254549	28.887946632413104	20.561018780361746
12-13	24.848036376015067	25.048170138129684	29.149735178610207	20.954058307245038
14-15	21.929675595792084	26.26972942809459	27.675943543931563	24.12465143218176
16-17	20.288130604358294	29.64123106527785	28.25964937197955	21.81098895838431
18-19	20.211666738208333	27.45252089428799	31.616231109785335	20.71958125771834
20-21	21.149323046889624	26.615515350760603	31.674191115375866	20.560970486973908
22-23	20.681968342458816	27.099030148866277	31.584748682617477	20.63425282605743
24-25	20.51254522934466	27.259780138445645	31.849894111323003	20.3777805208867
26-27	20.591918835503805	27.48773485546465	31.724791086130356	20.19555522290119
28-29	20.0489520283404	27.3728595753361	31.823808974888745	20.75437942143476
30-31	20.316479981986294	27.461500896039766	31.900476263181552	20.32154285879239
32-33	19.56746247951247	27.309294400721807	32.37051660131598	20.75272651844974
34-35	19.677783479942633	27.66911360279382	31.79495626154143	20.858146655722123
36-37	20.045661885853676	27.83241426886935	31.80687219568854	20.315051649588437
38-39	20.160400325705524	27.580549581379	31.511728700383017	20.747321392532463
40-41	20.296661816999475	27.67212570568434	31.464088186920424	20.56712429039576
42-43	20.806654339525895	27.64233754602526	31.09551085435162	20.45549726009723
44-45	20.4523250762566	28.41463342238682	30.284894483171392	20.848147018185188
46-47	21.218547393557063	27.9572697925713	29.09252894434668	21.731653869524955
48-49	21.113152884494767	28.136262683222256	29.014804884878686	21.735779547404295
50-51	20.75581209570821	28.437024962289755	29.094955763479057	21.712207178522974
52-53	20.892609532002922	28.726680990432445	29.166303641210728	21.214405836353905
54-55	20.90609620937778	28.845425944705244	28.601196316366533	21.64728152955044
56-57	21.52749628173973	29.150203799104997	27.810545833243705	21.51175408591157
58-59	20.76418465093461	29.496486794169073	28.807675756808187	20.931652798088123
60-61	21.36598384978626	29.950942139612373	27.703884709129234	20.979189301472132
62-63	20.971888124342364	30.283705385943883	27.569519450301883	21.17488703941187
64-65	21.063533612476046	29.911764392769218	27.710000246762107	21.314701747992626
66-67	21.20364406901567	29.509341932956634	27.718772066157783	21.568241931869913
68-69	21.43211221920759	28.97224167578803	28.015352736328776	21.58029336867561
70-71	21.452665082520824	28.40983549640525	28.153720555135877	21.983778865938046
72-73	21.5967149107831	28.495815789162513	28.186605042508635	21.720864257545752
74-75	21.506646950162764	28.575329835040776	28.17088958831514	21.747133626481325
76-77	21.477357001556946	28.437287779748544	27.99111014780976	22.09424507088475
78-79	21.658239019463306	28.322152479371105	28.031535955155213	21.98807254601038
80-81	21.59548046914324	28.186742801831816	28.187617280738582	22.03015944828636
82-83	21.84018279082553	28.09088840254622	28.219935860649493	21.848992945978758
84-85	22.058566493493935	28.02337001978052	28.0308102650849	21.887253221640652
86-87	21.609772575398196	28.171005382700454	28.14489230927396	22.07432973262739
88-89	21.984009760701404	27.881830708945586	28.07909267722256	22.05506685313045
90-91	22.041648680385084	27.775680331259657	28.181200776610915	22.00147021174434
92-93	22.09742939346519	27.779232914010564	28.099744588616062	22.02359310390818
94-95	21.82897638409832	27.72356851727088	28.290975815866116	22.156479282764685
96-97	21.978945560236323	27.90607469871752	28.27900895394535	21.835970787100806
98-99	23.06814881933411	29.82501452831407	28.359692476079886	18.747144176271938
100	29.621495204413694	40.82584350650219	29.552661289084114	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4109.0
1	4047.5
2	6948.5
3	10351.0
4	15174.0
5	27803.0
6	37476.0
7	41874.5
8	42643.0
9	38724.5
10	33286.5
11	28602.0
12	31644.5
13	44867.5
14	75423.5
15	117863.0
16	151465.5
17	168948.0
18	170841.0
19	158631.5
20	138066.5
21	115058.5
22	97371.0
23	90903.5
24	94134.5
25	106622.5
26	125899.5
27	145337.0
28	162993.5
29	184375.5
30	205187.0
31	221624.5
32	242724.0
33	268020.5
34	288312.5
35	308059.0
36	333347.0
37	356270.0
38	366866.0
39	368684.5
40	377774.0
41	389405.5
42	395968.5
43	403999.0
44	418361.0
45	435032.5
46	455768.5
47	479635.5
48	500442.5
49	488437.5
50	450287.0
51	418972.0
52	386031.0
53	348036.0
54	304281.5
55	258780.0
56	217328.5
57	181820.5
58	151673.0
59	123469.0
60	96205.0
61	73218.5
62	54816.0
63	39530.0
64	27691.0
65	20044.0
66	15184.5
67	11815.5
68	8932.0
69	6621.0
70	5050.0
71	3943.0
72	3117.0
73	2438.0
74	1776.0
75	1122.0
76	743.5
77	512.0
78	329.0
79	195.0
80	134.5
81	100.0
82	72.0
83	56.0
84	44.5
85	34.5
86	27.0
87	18.0
88	12.0
89	9.0
90	5.5
91	3.0
92	3.0
93	2.0
94	1.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.890553117314422E-6
2	2.8682604040211824E-4
3	9.890553117314422E-6
4	9.890553117314422E-6
5	9.890553117314422E-6
6	9.890553117314422E-6
7	9.890553117314422E-6
8	9.890553117314422E-6
9	0.0
10-11	0.0017456826252059956
12-13	0.00909436359137061
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.005759250583036328
36-37	0.0468749913182768
38-39	0.15141216800091303
40-41	0.17989299710609155
42-43	0.05631534633338341
44-45	0.005188804922824345
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.01560214907490037
64-65	0.10522765300102589
66-67	0.22679123617894936
68-69	0.2531424608912801
70-71	0.11827028696842702
72-73	0.008018794363038564
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	218.0
20-21	1074.0
22-23	2413.0
24-25	4560.0
26-27	9240.0
28-29	19931.0
30-31	40822.0
32-33	50882.0
34-35	64172.0
36-37	80717.0
38-39	97424.0
40-41	117166.0
42-43	144710.0
44-45	177797.0
46-47	168248.0
48-49	135631.0
50-51	114283.0
52-53	107679.0
54-55	118321.0
56-57	127021.0
58-59	127914.0
60-61	119343.0
62-63	113449.0
64-65	144713.0
66-67	164798.0
68-69	141301.0
70-71	120257.0
72-73	107766.0
74-75	104946.0
76-77	100872.0
78-79	107986.0
80-81	111566.0
82-83	105941.0
84-85	118480.0
86-87	122429.0
88-89	126767.0
90-91	125162.0
92-93	127468.0
94-95	132225.0
96-97	286026.0
98-99	1881686.0
100-101	4037254.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.17308629768806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42799708221631	67.47088363659898
2	7.39811478910161	11.418707021190816
3	2.271130940437123	5.258105521791104
4	1.0483388761364865	3.2361418622920954
5	0.5952769786848502	2.2969680823536476
6	0.3570747614059816	1.6533936826026112
7	0.22841595206970872	1.2339294786590944
8	0.16169626486707536	0.9982879842080504
9	0.10322097794671481	0.716929329493222
>10	0.40154578999263346	4.6738065532274735
>50	0.005142006702546107	0.25761831749064557
>100	0.0015168265292966848	0.1997166279257967
>500	2.4882539767990083E-4	0.14274749739864778
>1k	2.7992851199018574E-4	0.44276440476779827
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.901497805582979E-5
2	0.0	0.0	0.0	0.0	8.901497805582979E-5
3	0.0	0.0	0.0	0.0	8.901497805582979E-5
4	0.0	0.0	0.0	0.0	1.7802995611165958E-4
5	0.0	0.0	0.0	0.0	1.7802995611165958E-4
6	0.0	0.0	0.0	0.0	2.670449341674894E-4
7	0.0	0.0	0.0	9.890553117314422E-6	2.670449341674894E-4
8	0.0	0.0	0.0	9.890553117314422E-6	3.7584101845794803E-4
9	0.0	0.0	0.0	9.890553117314422E-6	4.648559965137778E-4
10-11	0.0	0.0	9.890553117314422E-6	9.890553117314422E-6	4.6980127307243505E-4
12-13	0.0	0.0	9.890553117314422E-6	1.9781106234628844E-5	4.994729324243783E-4
14-15	0.0	0.0	9.890553117314422E-6	2.9671659351943267E-5	6.824481650946951E-4
16-17	0.0	0.0	9.890553117314422E-6	2.9671659351943267E-5	8.753139508823264E-4
18-19	0.0	0.0	9.890553117314422E-6	3.461693591060048E-5	9.940005882900995E-4
20-21	0.0	0.0	9.890553117314422E-6	3.956221246925769E-5	0.0010038911414074138
22-23	0.0	0.0	9.890553117314422E-6	1.0385080773180144E-4	0.0011769758209604162
24-25	0.0	0.0	9.890553117314422E-6	3.1155242319540427E-4	0.0013747868833067045
26-27	0.0	0.0	9.890553117314422E-6	6.775028885360379E-4	0.0014242396488932767
28-29	0.0	0.0	9.890553117314422E-6	0.0015973243284462793	0.0014440207551279056
30-31	4.945276558657211E-6	0.0	9.890553117314422E-6	0.003842479886076653	0.001478637691038506
32-33	9.890553117314422E-6	0.0	9.890553117314422E-6	0.007160760456935641	0.001661612923708823
34-35	9.890553117314422E-6	0.0	9.890553117314422E-6	0.010805429280666006	0.0017704090079992815
36-37	9.890553117314422E-6	0.0	9.890553117314422E-6	0.015364974267747956	0.0017901901142339104
38-39	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.0217048188159465	0.0018594239860551115
40-41	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.02798532004544116	0.0018989861985243691
42-43	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.034676279229304365	0.0019237125813176552
44-45	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.04259861227627322	0.00196822007034557
46-47	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.0515001100818562	0.002042399218725428
48-49	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.05947189589441162	0.0020967972608706576
50-51	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.06675134298875504	0.002101742537429315
52-53	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.07380825263795887	0.002299553599775603
54-55	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.07981676365672738	0.002512200491797863
56-57	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.08573625969744006	0.0025616532573844355
58-59	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.09164091990847678	0.0026160512995296644
60-61	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.09744667458834033	0.0026852851713508657
62-63	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.10208039872380215	0.0027594643197307238
64-65	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.10675863034829186	0.002769354872848038
66-67	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.11335562927754059	0.0028039718087586388
68-69	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.1207389271796158	0.0028237529149932675
70-71	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.12661886100785924	0.002922658446166412
72-73	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.13166798837424826	0.0031353053381886716
74-75	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.13549563243064894	0.0031353053381886716
76-77	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.13916997291373123	0.0031451958913059864
78-79	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14165250174617716	0.0031550864444233008
80-81	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14237945740029978	0.003189703380333901
82-83	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14283936812025488	0.003283663634948388
84-85	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14316575637312626	0.0032886089115070453
86-87	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14332895049956196	0.0032984994646243597
88	1.9781106234628844E-5	0.0	9.890553117314422E-6	0.14335367688235523	0.0033331164005349604
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	15095	0.0	48.53765	1
TATACAC	17425	0.0	44.23976	3
GGTATCA	31930	0.0	41.904137	1
TTATACA	20025	0.0	37.363148	2
GTATCAA	56095	0.0	33.4586	1
TACACAT	25745	0.0	29.619072	5
ATACACA	27985	0.0	27.958519	4
ACACATC	26725	0.0	27.883492	6
TATCAAC	70275	0.0	26.503492	2
ATCAACG	70450	0.0	26.40928	3
AACGCAG	70555	0.0	26.387524	6
ACATCTC	28390	0.0	26.263649	8
TCAACGC	71495	0.0	26.023272	4
CAACGCA	71480	0.0	25.984964	5
CACATCT	28760	0.0	25.925768	7
ACGCAGA	78235	0.0	23.767939	7
CGCAGAG	78755	0.0	23.590782	8
CATCTCC	32255	0.0	23.005491	9
TGCGCGA	625	7.2759576E-12	22.302382	94
GCAGAGT	85125	0.0	21.774015	9
>>END_MODULE