FastQCFastQC Report
Fri 27 May 2016
SRR2138763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10086439
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC179900.053.881681
TATACAC208400.047.6929443
TTATACA226150.043.518292
GGTATCA334300.042.0282251
ATACACA311750.032.3703584
GTATCAA569400.031.9837761
TACACAT314450.031.8571625
ACACATC338900.028.929276
CACATCT366700.026.7691087
ACATCTC371600.026.5422178
TATCAAC723500.025.0365832
ATCAACG733050.024.635733
TCAACGC737300.024.5149024
AACGCAG736350.024.49216
CAACGCA738850.024.421695
CATCTCC412550.023.8602969
ACGCAGA820200.021.9843547
CGCAGAG821900.021.9151298
ACGGTAC9800.021.9093783
GCAGAGT889900.020.1615479
Produced by FastQC (version 0.11.5)