##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10086439
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66088725664231	33.0	33.0	33.0	33.0	33.0
2	32.602015141319946	33.0	33.0	33.0	33.0	33.0
3	32.669191277516276	33.0	33.0	33.0	33.0	33.0
4	36.638561934494426	37.0	37.0	37.0	37.0	37.0
5	36.64435724044928	37.0	37.0	37.0	37.0	37.0
6	36.6369924013817	37.0	37.0	37.0	37.0	37.0
7	36.61805092956989	37.0	37.0	37.0	37.0	37.0
8	36.614244035977414	37.0	37.0	37.0	37.0	37.0
9	36.61901965599554	37.0	37.0	37.0	37.0	37.0
10-11	36.62237802657608	37.0	37.0	37.0	37.0	37.0
12-13	36.61920168257598	37.0	37.0	37.0	37.0	37.0
14-15	38.74021857466248	40.0	37.0	40.0	37.0	40.0
16-17	38.656098103602275	40.0	37.0	40.0	37.0	40.0
18-19	38.73290613267973	40.0	37.0	40.0	37.0	40.0
20-21	38.76958041549766	40.0	37.0	40.0	37.0	40.0
22-23	38.76942043276064	40.0	37.0	40.0	37.0	40.0
24-25	38.75758913576652	40.0	37.0	40.0	37.0	40.0
26-27	38.66476584360069	40.0	37.0	40.0	37.0	40.0
28-29	38.54737612467984	40.0	37.0	40.0	37.0	40.0
30-31	38.37071467141267	40.0	37.0	40.0	37.0	40.0
32-33	38.23583898303143	40.0	37.0	40.0	37.0	40.0
34-35	38.17508980315673	40.0	37.0	40.0	37.0	40.0
36-37	38.161711006359354	40.0	37.0	40.0	37.0	40.0
38-39	38.09676343002023	40.0	37.0	40.0	37.0	40.0
40-41	38.095366560806234	40.0	37.0	40.0	37.0	40.0
42-43	38.0717574736326	40.0	37.0	40.0	37.0	40.0
44-45	37.97061266099882	40.0	37.0	40.0	33.0	40.0
46-47	37.601192980309875	40.0	37.0	40.0	33.0	40.0
48-49	37.79248877473279	40.0	37.0	40.0	33.0	40.0
50-51	37.77539332802711	40.0	37.0	40.0	33.0	40.0
52-53	37.77066431600788	40.0	37.0	40.0	33.0	40.0
54-55	37.63929285740206	40.0	37.0	40.0	33.0	40.0
56-57	37.4558766848266	40.0	37.0	40.0	33.0	40.0
58-59	37.29799607259301	38.5	37.0	40.0	33.0	40.0
60-61	37.100224028531535	37.0	37.0	40.0	33.0	40.0
62-63	36.97962134836864	37.0	37.0	40.0	33.0	40.0
64-65	36.84445230598594	37.0	37.0	40.0	33.0	40.0
66-67	36.65332861572238	37.0	37.0	40.0	33.0	40.0
68-69	36.48419882129657	37.0	37.0	40.0	33.0	40.0
70-71	36.28728689192211	37.0	37.0	38.5	33.0	40.0
72-73	36.02528426606978	37.0	37.0	37.0	33.0	40.0
74-75	35.70886341082114	37.0	35.0	37.0	33.0	40.0
76-77	33.517315033188495	35.0	33.0	37.0	27.0	37.0
78-79	35.22068995507982	37.0	33.0	37.0	33.0	37.0
80-81	35.439168692111025	37.0	33.0	37.0	33.0	37.0
82-83	35.275590163724075	37.0	33.0	37.0	33.0	37.0
84-85	35.13642646751772	37.0	33.0	37.0	33.0	37.0
86-87	35.050510172088124	37.0	33.0	37.0	33.0	37.0
88-89	34.88453885222434	37.0	33.0	37.0	33.0	37.0
90-91	34.83088121503712	37.0	33.0	37.0	33.0	37.0
92-93	34.881597255118336	37.0	33.0	37.0	33.0	37.0
94-95	34.87090022535829	37.0	33.0	37.0	33.0	37.0
96-97	34.872013533308866	37.0	33.0	37.0	33.0	37.0
98-99	34.88448790277424	37.0	33.0	37.0	33.0	37.0
100	34.732593182136334	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	5.0
18	8.0
19	23.0
20	86.0
21	294.0
22	1098.0
23	3049.0
24	6923.0
25	13618.0
26	23989.0
27	39221.0
28	58536.0
29	82184.0
30	113173.0
31	153257.0
32	207440.0
33	281930.0
34	399097.0
35	608081.0
36	1174735.0
37	3167846.0
38	3601318.0
39	150526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15605761599614	20.83757045973131	15.090098731613299	22.91627319265925
2	14.433078306585912	20.80839998996668	43.16134618193045	21.59717552151696
3	17.158579815365886	27.841739920992897	32.37892278094292	22.6207574826983
4	11.546309474060955	19.055255895665812	38.2048265814433	31.19360804882994
5	12.023643636230243	38.25687668636144	35.87057270482584	13.848906972582483
6	27.649733994273696	38.77850027715436	18.534076998646796	15.037688729925147
7	24.084312428660386	34.52407425932468	22.816292809839588	18.575320502175348
8	24.018669823645354	36.05041106190422	21.60625203924835	18.324667075202075
9	25.010950347387258	16.41275145893922	21.353911063548896	37.22238713012463
10-11	23.342256183438952	26.999155734031284	28.756350274553743	20.902237807976014
12-13	24.849045116196727	24.899523293061016	28.862754239445525	21.388677351296728
14-15	22.090893525455314	26.158171382387778	27.327974719323638	24.422960372833266
16-17	20.397163954493752	29.67392158917533	27.942557328706396	21.986357127624526
18-19	20.055517115604427	27.14653803983745	31.65494283958888	21.143002004969247
20-21	20.9714122347412	26.14948846544392	31.545307640297164	21.333791659517715
22-23	20.854914303002815	26.94770658058971	31.45046923889381	20.74690987751367
24-25	20.58428617384108	27.281726045173514	31.79043252208845	20.343555258896956
26-27	20.22571443757419	27.31424196275254	32.07078200059146	20.389261599081813
28-29	19.52828632772046	26.881538441585406	32.59666976360799	20.99350546708615
30-31	20.266335914642376	26.8417617090343	31.503631953053624	21.3882704232697
32-33	20.60888627921331	27.111442189356445	32.15960697951892	20.12006455191133
34-35	20.355258208288152	28.02159275854159	31.72630623593048	19.89684279723978
36-37	20.143252416594905	27.566955470100336	31.17949992876848	21.110292184536288
38-39	20.21218051952386	27.258105947403266	31.5433527750003	20.986360758072575
40-41	20.20008413167965	27.35038965702249	31.710132961456	20.73939324984186
42-43	20.900845361334017	27.239217428957634	31.30237097110217	20.557566238606174
44-45	20.399055786083288	28.121939049106004	30.520262643485584	20.958742521325124
46-47	21.42643079412918	27.581051416625048	29.048135172693414	21.94438261655236
48-49	21.2981106337105	27.683623958142807	29.043194587950815	21.97507082019588
50-51	20.695798575311517	28.134404943558877	29.181729433360488	21.988067047769118
52-53	20.9053765720966	28.564371793121595	29.35216118744212	21.178090447339688
54-55	20.90708768290629	28.65459994544842	28.577801536354574	21.860510835290714
56-57	21.676654493051252	29.04820443290757	27.6717140362784	21.603427037762778
58-59	20.7683456548455	29.555088359289826	28.907238786658485	20.769327199206185
60-61	21.47685002046968	30.279582879873512	27.331394048658257	20.91217305099855
62-63	20.863353598687688	30.684245944374393	27.251808880135215	21.20059157680271
64-65	20.938075789072386	30.230697311792703	27.48784944249629	21.343377456638617
66-67	21.277279158463962	29.6819458597478	27.518729214515552	21.522045767272687
68-69	21.43960550961509	28.97556395836093	27.836445311207076	21.748385220816903
70-71	21.56888455455119	28.411610925809068	27.92830723169676	22.091197287942986
72-73	21.736404995631165	28.405883531526698	27.98674966669096	21.870961806151175
74-75	21.607344305132774	28.436105359828286	27.986233401823558	21.970316933215386
76-77	21.624519653568665	28.28562099767832	27.89487509332756	22.194984255425457
78-79	21.779285262586033	28.270488637633907	27.87684703333283	22.07337906644723
80-81	21.687662569565504	28.11956324128927	28.016255632754138	22.176518556391084
82-83	21.955249323744173	27.913094549055156	28.116300157135303	22.015355970065364
84-85	22.190925447438158	27.81992764352595	27.911656219695452	22.07749068934044
86-87	21.845173505383396	28.063879927948104	27.934757304380582	22.156189262287917
88-89	22.099570343469274	27.76060640974744	27.83761964753469	22.302203599248596
90-91	22.22923495141014	27.652500552061642	27.990019271750967	22.12824522477725
92-93	22.168223058253123	27.691113672656613	27.96928494771483	22.171378321375432
94-95	21.995863049617878	27.621404177876315	28.113569875167542	22.269162897338266
96-97	22.164423208181766	27.703459522927904	28.04009612361463	22.092021145275694
98-99	23.193924918591406	29.613479594398584	28.21578846206855	18.976807024941465
100	30.008708586372556	40.553071257837594	29.438220155789846	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4656.0
1	4468.0
2	7424.5
3	11074.5
4	16214.5
5	29384.0
6	39524.5
7	44292.5
8	45606.0
9	42010.5
10	36411.0
11	31335.5
12	34127.5
13	47427.0
14	79077.5
15	123599.0
16	159350.5
17	178477.5
18	179955.5
19	166020.0
20	143998.5
21	119522.0
22	100269.0
23	93503.0
24	98527.0
25	111765.5
26	130491.0
27	148905.0
28	163925.5
29	180871.0
30	197533.0
31	212963.5
32	234495.0
33	259388.5
34	279908.5
35	300638.0
36	321895.5
37	341094.0
38	353599.0
39	357113.5
40	367318.0
41	377757.5
42	386648.5
43	401244.5
44	416041.5
45	432623.5
46	460207.0
47	492408.0
48	525018.5
49	523409.0
50	487939.5
51	461315.5
52	426428.5
53	379799.5
54	330843.5
55	281828.0
56	238561.5
57	200238.0
58	166949.0
59	135258.0
60	105529.0
61	81977.0
62	61528.5
63	44577.5
64	32132.0
65	23596.0
66	17628.5
67	13087.5
68	9435.0
69	6765.5
70	5003.0
71	3871.5
72	3130.0
73	2511.0
74	1815.0
75	1151.5
76	781.5
77	530.0
78	322.0
79	212.5
80	152.5
81	108.0
82	79.5
83	49.0
84	27.5
85	20.5
86	19.5
87	16.5
88	14.0
89	11.0
90	7.0
91	4.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.965720706782642E-5
2	4.560578812800038E-4
3	3.965720706782642E-5
4	3.965720706782642E-5
5	3.965720706782642E-5
6	1.982860353391321E-5
7	1.982860353391321E-5
8	1.982860353391321E-5
9	9.914301766956604E-6
10-11	0.0016110740371304483
12-13	0.008848514327008769
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.962741720260969E-6
28-29	2.4845755067962582E-5
30-31	3.9868839491839744E-5
32-33	4.0063233805076244E-5
34-35	0.005952437954276607
36-37	0.046333688063452644
38-39	0.1507142497932114
40-41	0.1810609373856322
42-43	0.05561492418905078
44-45	0.005158137012287511
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.014748295340769792
64-65	0.10531163645562572
66-67	0.23005665356314373
68-69	0.25555612923203314
70-71	0.11994942820240588
72-73	0.00810517496717404
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	222.0
20-21	1333.0
22-23	2796.0
24-25	5052.0
26-27	10318.0
28-29	22826.0
30-31	45944.0
32-33	59403.0
34-35	79358.0
36-37	104530.0
38-39	125146.0
40-41	132910.0
42-43	162476.0
44-45	196253.0
46-47	178864.0
48-49	148577.0
50-51	124164.0
52-53	113522.0
54-55	136600.0
56-57	147438.0
58-59	144903.0
60-61	147778.0
62-63	147920.0
64-65	164867.0
66-67	176217.0
68-69	145755.0
70-71	117307.0
72-73	108491.0
74-75	107063.0
76-77	100716.0
78-79	102987.0
80-81	106492.0
82-83	114090.0
84-85	111949.0
86-87	111972.0
88-89	124623.0
90-91	123710.0
92-93	124760.0
94-95	129504.0
96-97	271746.0
98-99	1791231.0
100-101	3814626.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.9268271784714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64499143958136	68.29896100970862
2	7.410405992181936	11.549388541501413
3	2.184050367048214	5.1058834650612965
4	0.986666367639141	3.0755111805530184
5	0.553944973320408	2.1583587101161195
6	0.3438902453694747	1.6078965431561505
7	0.2143746931461991	1.1693877764969185
8	0.1568304855757491	0.9777041716621723
9	0.10390970449621667	0.7287618225998439
>10	0.39324174954044794	4.728350239201529
>50	0.006113343527933359	0.3147721174026968
>100	0.0014344731386786345	0.19417617487083946
>500	1.169323994109479E-4	0.0677717262674054
>1k	2.9233034799681433E-5	0.023076521401965967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9742905300869813E-5
2	0.0	0.0	0.0	0.0	2.9742905300869813E-5
3	0.0	0.0	0.0	0.0	2.9742905300869813E-5
4	0.0	0.0	0.0	0.0	3.965720706782642E-5
5	0.0	0.0	0.0	0.0	3.965720706782642E-5
6	0.0	0.0	0.0	0.0	6.940011236869622E-5
7	0.0	0.0	0.0	0.0	6.940011236869622E-5
8	0.0	0.0	0.0	0.0	1.0905731943652264E-4
9	0.0	0.0	0.0	0.0	1.8837173357217547E-4
10-11	0.0	0.0	0.0	0.0	1.8837173357217547E-4
12-13	0.0	0.0	0.0	0.0	2.1811463887304528E-4
14-15	0.0	0.0	0.0	9.914301766956604E-6	3.222148074260896E-4
16-17	0.0	0.0	0.0	9.914301766956604E-6	4.064863724452208E-4
18-19	0.0	0.0	0.0	4.957150883478302E-5	4.858007865808736E-4
20-21	0.0	0.0	0.0	8.922871590260943E-5	4.907579374643519E-4
22-23	0.0	0.0	0.0	9.914301766956603E-5	5.254579936487E-4
24-25	0.0	0.0	0.0	1.6854313003826228E-4	5.552008989495698E-4
26-27	0.0	0.0	0.0	3.1725765654261134E-4	5.998152569008746E-4
28-29	0.0	0.0	0.0	0.001016215931113052	6.146867095513094E-4
30-31	0.0	0.0	0.0	0.0026223328173600215	6.24601011318266E-4
32-33	0.0	0.0	0.0	0.005264494238253956	6.543439166191359E-4
34-35	0.0	0.0	0.0	0.00864031398990268	6.940011236869622E-4
36-37	0.0	0.0	0.0	0.013587550571614026	6.989582745704406E-4
38-39	0.0	0.0	0.0	0.021380191760441918	7.23744028987832E-4
40-41	0.0	0.0	0.0	0.02951983351111329	7.435726325217453E-4
42-43	0.0	0.0	0.0	0.03698034559074813	7.485297834052237E-4
44-45	0.0	0.0	0.0	0.04597757444426125	7.534869342887019E-4
46-47	0.0	0.0	0.0	0.05608520509567351	7.534869342887019E-4
48-49	0.0	0.0	0.0	0.06526584853187532	7.733155378226151E-4
50-51	0.0	0.0	0.0	0.07405487704828234	7.782726887060934E-4
52-53	0.0	0.0	0.0	0.08236306192899198	8.575871028417462E-4
54-55	0.0	0.0	0.0	0.09020031747577117	9.468158187443557E-4
56-57	0.0	0.0	0.0	0.09822098760523906	9.666444222782689E-4
58-59	0.0	0.0	0.0	0.10663327265450176	9.716015731617472E-4
60-61	0.0	0.0	0.0	0.1141383990920879	9.914301766956604E-4
62-63	0.0	0.0	0.0	0.1215840397190723	0.0010112587802295736
64-65	0.0	0.0	0.0	0.1291932663252115	0.0010112587802295736
66-67	0.0	0.0	0.0	0.13704539332464113	0.001036044534646965
68-69	0.0	0.0	0.0	0.14429770506716988	0.001065787439947835
70-71	0.0	0.0	0.0	0.15004304294112125	0.0011203160996660962
72-73	0.0	0.0	0.0	0.15468293616805695	0.001249202022636532
74-75	0.0	0.0	0.0	0.1582570419550448	0.001249202022636532
76-77	0.0	0.0	0.0	0.161751833327897	0.0012591163244034887
78-79	0.0	0.0	0.0	0.16396272262192832	0.0012739877770539237
80-81	0.0	0.0	0.0	0.16466168089649874	0.0012888592297043585
82-83	0.0	0.0	0.0	0.16530611051135094	0.0012888592297043585
84-85	0.0	0.0	0.0	0.16562832531877703	0.0012888592297043585
86-87	0.0	0.0	0.0	0.16578199699616486	0.0012888592297043585
88	0.0	0.0	0.0	0.16579686844881528	0.0012888592297043585
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	17990	0.0	53.88168	1
TATACAC	20840	0.0	47.692944	3
TTATACA	22615	0.0	43.51829	2
GGTATCA	33430	0.0	42.028225	1
ATACACA	31175	0.0	32.370358	4
GTATCAA	56940	0.0	31.983776	1
TACACAT	31445	0.0	31.857162	5
ACACATC	33890	0.0	28.92927	6
CACATCT	36670	0.0	26.769108	7
ACATCTC	37160	0.0	26.542217	8
TATCAAC	72350	0.0	25.036583	2
ATCAACG	73305	0.0	24.63573	3
TCAACGC	73730	0.0	24.514902	4
AACGCAG	73635	0.0	24.4921	6
CAACGCA	73885	0.0	24.42169	5
CATCTCC	41255	0.0	23.860296	9
ACGCAGA	82020	0.0	21.984354	7
CGCAGAG	82190	0.0	21.915129	8
ACGGTAC	980	0.0	21.909378	3
GCAGAGT	88990	0.0	20.161547	9
>>END_MODULE