FastQCFastQC Report
Fri 27 May 2016
SRR2138762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8162299
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216800.048.3572041
GTATCAA392950.035.8735661
TATCAAC492600.028.4389232
ATCAACG493200.028.3619083
AACGCAG495750.028.2714756
TCAACGC497550.028.1306934
CAACGCA497450.028.0872965
ACGCAGA548500.025.4857487
CGCAGAG552600.025.2891128
GCAGAGT611050.022.9110079
GTACATG339550.021.4511
GAGTACT425900.021.21850812-13
TACATGG344900.020.7316532
ACATGGG337150.020.7137153
ACGGTAC7450.020.694393
CAGAGTA602250.020.22500610-11
GTACTTT467750.019.25088914-15
GTGGTAT102600.019.0879961
AGAGTAC584750.019.07690610-11
AGTACTT447400.018.63863812-13
Produced by FastQC (version 0.11.5)