##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8162299
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.724573922126595	33.0	33.0	33.0	33.0	33.0
2	32.65329241185602	33.0	33.0	33.0	33.0	33.0
3	32.73009393554439	33.0	33.0	33.0	33.0	33.0
4	36.71245184720628	37.0	37.0	37.0	37.0	37.0
5	36.716790085734424	37.0	37.0	37.0	37.0	37.0
6	36.7130320758894	37.0	37.0	37.0	37.0	37.0
7	36.70008449825227	37.0	37.0	37.0	37.0	37.0
8	36.69350179404112	37.0	37.0	37.0	37.0	37.0
9	36.68459082422734	37.0	37.0	37.0	37.0	37.0
10-11	36.692594084093216	37.0	37.0	37.0	37.0	37.0
12-13	36.69161127275538	37.0	37.0	37.0	37.0	37.0
14-15	38.92407329111565	40.0	38.5	40.0	37.0	40.0
16-17	38.863252559113555	40.0	37.0	40.0	37.0	40.0
18-19	38.916642480262	40.0	38.5	40.0	37.0	40.0
20-21	38.936542639344225	40.0	40.0	40.0	37.0	40.0
22-23	38.94099712420924	40.0	40.0	40.0	37.0	40.0
24-25	38.93530635272388	40.0	40.0	40.0	37.0	40.0
26-27	38.85536615262029	40.0	37.0	40.0	37.0	40.0
28-29	38.75242781012466	40.0	37.0	40.0	37.0	40.0
30-31	38.58851991447446	40.0	37.0	40.0	37.0	40.0
32-33	38.47926731100721	40.0	37.0	40.0	37.0	40.0
34-35	38.44565363947497	40.0	37.0	40.0	37.0	40.0
36-37	38.41190246971687	40.0	37.0	40.0	37.0	40.0
38-39	38.36604782483957	40.0	37.0	40.0	37.0	40.0
40-41	38.332784610863456	40.0	37.0	40.0	37.0	40.0
42-43	38.29672020574351	40.0	37.0	40.0	37.0	40.0
44-45	38.19817658882543	40.0	37.0	40.0	37.0	40.0
46-47	37.801221935168456	40.0	37.0	40.0	33.0	40.0
48-49	37.985831082174165	40.0	37.0	40.0	33.0	40.0
50-51	38.000515355130176	40.0	37.0	40.0	33.0	40.0
52-53	37.967120347089605	40.0	37.0	40.0	33.0	40.0
54-55	37.874115799814675	40.0	37.0	40.0	33.0	40.0
56-57	37.69823570438261	40.0	37.0	40.0	33.0	40.0
58-59	37.5292415812271	40.0	37.0	40.0	33.0	40.0
60-61	37.35018039677129	37.0	37.0	40.0	33.0	40.0
62-63	37.20276109464335	37.0	37.0	40.0	33.0	40.0
64-65	37.02650404645597	37.0	37.0	40.0	33.0	40.0
66-67	36.81928214653198	37.0	37.0	40.0	33.0	40.0
68-69	36.61721168896648	37.0	37.0	40.0	33.0	40.0
70-71	36.39480108696088	37.0	37.0	38.5	33.0	40.0
72-73	36.116964164901496	37.0	37.0	37.0	33.0	40.0
74-75	35.784297329548096	37.0	37.0	37.0	33.0	40.0
76-77	33.62799899913581	35.0	33.0	37.0	30.0	37.0
78-79	35.282131589886646	37.0	33.0	37.0	33.0	37.0
80-81	35.48514185046887	37.0	33.0	37.0	33.0	37.0
82-83	35.320651638592935	37.0	33.0	37.0	33.0	37.0
84-85	35.175608786964105	37.0	33.0	37.0	33.0	37.0
86-87	35.081182475196584	37.0	33.0	37.0	33.0	37.0
88-89	34.90976370706872	37.0	33.0	37.0	33.0	37.0
90-91	34.85291259034463	37.0	33.0	37.0	33.0	37.0
92-93	34.90134816019835	37.0	33.0	37.0	33.0	37.0
94-95	34.89092575194067	37.0	33.0	37.0	33.0	37.0
96-97	34.88899561325094	37.0	33.0	37.0	33.0	37.0
98-99	34.89742606732413	37.0	33.0	37.0	33.0	37.0
100	34.84307191416969	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	4.0
18	5.0
19	15.0
20	38.0
21	178.0
22	639.0
23	1787.0
24	4275.0
25	8699.0
26	16250.0
27	26758.0
28	40671.0
29	58628.0
30	81322.0
31	111551.0
32	150380.0
33	207652.0
34	297985.0
35	463778.0
36	918501.0
37	2580750.0
38	3075776.0
39	116654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60676162687558	20.217203590754366	14.641578791065651	22.53445599130441
2	14.887287151110124	20.892178451862392	42.36015430066675	21.860380096360732
3	18.013767204717887	27.055426561349893	30.889580578797926	24.04122565513429
4	12.181694954458893	17.98730406248438	39.14154791739971	30.68945306565702
5	12.764815194156153	38.02758439537111	35.434465988491475	13.773134421981267
6	28.945332422968683	38.204772504602566	18.761446685902264	14.088448386526489
7	25.471634891101147	33.720181042130406	23.455340217259867	17.352843849508577
8	24.176240051975554	36.30474698366232	21.267929537989236	18.251083426372887
9	25.800108523346182	16.67728663211186	21.893500838427997	35.62910400611396
10-11	24.03484105235469	27.02187155766377	28.59614368904871	20.34714370093283
12-13	25.160376481162892	24.935530229023144	29.082829213030763	20.8212640767832
14-15	22.15699277862769	25.931070400631	27.894598078311024	24.01733874243029
16-17	20.62344812411307	28.96308993336314	28.535391805666517	21.878070136857275
18-19	20.4472906469121	27.411982824936942	31.605605234505624	20.535121293645332
20-21	21.514233873390932	26.595358292754838	31.699289450342903	20.191118383511323
22-23	21.245931834074582	26.477004148760365	31.540742679445614	20.736321337719435
24-25	20.73056313616199	26.753902110140572	31.720831874149575	20.794702879547867
26-27	20.740152313403577	27.020008881719864	31.48169377487562	20.75814503000094
28-29	20.5999753962598	27.273264416273197	31.44413217669167	20.682628010775332
30-31	20.93956769571817	27.089234559386906	31.31529458262761	20.65590316226731
32-33	20.66087058786692	27.297714067974777	31.178471782454487	20.862943561703815
34-35	20.766471553835466	27.425546433937082	31.10650959109788	20.701472421129573
36-37	20.873370161571764	27.395364433484847	30.911830990308403	20.819434414634983
38-39	20.837279313714465	27.569614602627183	30.767828181830524	20.825277901827825
40-41	21.02455971033026	27.502966942967532	30.634207195442887	20.838266151259322
42-43	21.13709060281004	27.611752972578422	30.111530234935152	21.139626189676385
44-45	21.21761478486861	28.00028876696506	29.57617351686078	21.205922931305548
46-47	21.32910740673006	28.079359237436158	28.945572408515048	21.645960947318738
48-49	21.579375935457424	28.172340145057746	28.647218028107524	21.601065891377306
50-51	21.527174333841057	28.220132895121807	28.44746607997639	21.805226691060742
52-53	21.591223003473747	28.35821285619907	28.190054418943973	21.86050972138321
54-55	21.725626073186042	28.292786602192916	28.15430815056279	21.827279174058255
56-57	21.79256861497116	28.286228150341326	27.950418697961965	21.970784536725553
58-59	21.74160932107006	28.15575169887643	28.20242675869607	21.900212221357435
60-61	21.79348359623996	28.28590701710269	28.10655435295921	21.81405503369814
62-63	21.817264535956415	28.249842267946097	28.023111602810374	21.909781593287114
64-65	21.769389669560645	28.209527398222324	27.957603986098523	22.06347894611851
66-67	21.894579828809615	28.17758572384258	27.77966385161213	22.148170595735678
68-69	21.9063102421358	28.072800866334713	27.93191858853405	22.088970302995435
70-71	21.883196245059434	27.838328459056438	27.906959353002975	22.37151594288115
72-73	22.030150601656544	27.919528655075844	27.924276944893943	22.126043798373665
74-75	22.0007489096061	27.96487534292147	27.801935276806113	22.23244047066632
76-77	21.98552465388259	27.862724557196668	27.715687092771997	22.436063696148743
78-79	22.071773182414354	27.825222112441956	27.714386414980964	22.388618290162725
80-81	22.048072062436503	27.805797740072517	27.834471991445486	22.31165820604549
82-83	22.267709990345853	27.620159098505585	27.8514743232035	22.260656587945054
84-85	22.394594389174923	27.62170505494428	27.64565313147358	22.338047424407222
86-87	22.088750722721116	27.72753849533005	27.78770894598076	22.396001835968075
88-89	22.39295227575037	27.4240436432102	27.64481060250848	22.538193478530946
90-91	22.3989577484104	27.380222524690385	27.769769615967082	22.451050110932137
92-93	22.500912451324496	27.377836145695838	27.763466889679833	22.357784513299837
94-95	22.2867091976583	27.336595182662133	27.874121291890887	22.50257432778868
96-97	22.436565687074367	27.444147869080695	27.810371281178764	22.308915162666175
98-99	23.526828067767344	29.344606282034757	28.054035389820616	19.07453026037728
100	22.39441089434816	30.136057137642663	21.784092908961732	25.685439059047443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3245.0
1	3174.0
2	5148.0
3	7567.0
4	10827.5
5	19010.0
6	25360.0
7	28801.5
8	30226.0
9	28212.5
10	24319.0
11	20676.0
12	22585.0
13	31610.5
14	52475.5
15	81035.5
16	103749.5
17	116247.0
18	117298.0
19	107346.0
20	91900.0
21	75122.0
22	61764.5
23	56342.0
24	57918.0
25	65933.0
26	77514.0
27	89944.5
28	103849.0
29	120374.0
30	137682.5
31	154075.0
32	173139.5
33	191195.0
34	207989.5
35	226736.0
36	247349.0
37	268206.0
38	281756.0
39	287287.0
40	293990.0
41	303303.0
42	312228.0
43	323343.0
44	335305.0
45	348037.5
46	355709.0
47	356367.0
48	353558.5
49	343824.0
50	330599.5
51	315194.0
52	294884.5
53	272237.0
54	247731.5
55	219193.0
56	190055.0
57	162435.5
58	137697.0
59	116723.0
60	94812.0
61	73969.5
62	57044.0
63	43586.5
64	32853.0
65	24090.0
66	18272.5
67	14284.0
68	10739.5
69	7876.5
70	5940.5
71	4278.0
72	3114.0
73	2379.0
74	1814.0
75	1288.0
76	771.0
77	492.0
78	338.5
79	242.0
80	173.5
81	118.5
82	90.0
83	59.0
84	36.0
85	26.5
86	23.5
87	23.5
88	19.0
89	13.0
90	9.0
91	5.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.675435070437875E-5
2	6.493268624440247E-4
3	3.675435070437875E-5
4	3.675435070437875E-5
5	3.675435070437875E-5
6	2.450290046958584E-5
7	0.0
8	0.0
9	0.0
10-11	0.001788711734279766
12-13	0.008845547069520487
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.2271190612882776E-5
30-31	1.2293419080750982E-5
32-33	1.2327534707557592E-5
34-35	0.006127884434159437
36-37	0.048364064407172266
38-39	0.15449031845885036
40-41	0.18098974422923655
42-43	0.05592954833110699
44-45	0.005043541937051207
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.014748337042985645
64-65	0.10088466027678827
66-67	0.22054054362441716
68-69	0.243450148735739
70-71	0.1105551294108721
72-73	0.0071293456180638185
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	635.0
22-23	1491.0
24-25	2895.0
26-27	5559.0
28-29	11627.0
30-31	22163.0
32-33	24662.0
34-35	31751.0
36-37	45248.0
38-39	56010.0
40-41	70523.0
42-43	75456.0
44-45	93793.0
46-47	95352.0
48-49	77322.0
50-51	71573.0
52-53	59838.0
54-55	66396.0
56-57	72986.0
58-59	65715.0
60-61	34059.0
62-63	59909.0
64-65	79981.0
66-67	97067.0
68-69	89801.0
70-71	89448.0
72-73	86515.0
74-75	83100.0
76-77	79477.0
78-79	84598.0
80-81	83196.0
82-83	85596.0
84-85	92388.0
86-87	98674.0
88-89	105454.0
90-91	105010.0
92-93	107109.0
94-95	114119.0
96-97	257961.0
98-99	468311.0
100-101	4909408.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.07741123812289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38706101526266	66.58491027526215
2	8.148427167707826	12.561193434986183
3	2.553577918081182	5.904695259615989
4	1.1385379737583965	3.510222384543804
5	0.6005582518255487	2.3144737674202993
6	0.354885390822461	1.6412188326494876
7	0.2232248416074496	1.2043915040599569
8	0.15316668367645178	0.9444553172567488
9	0.10545915061048376	0.7315666488384658
>10	0.3295639410549663	3.8022619956925845
>50	0.0037878140932624082	0.19476706092869644
>100	0.0012468210772623015	0.17250788283501975
>500	2.5151522452848446E-4	0.129333261329406
>1k	2.515151975237951E-4	0.30400237458112755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.675435070437875E-5	0.0	0.0	0.0	0.0
6	7.35087014087575E-5	0.0	0.0	0.0	0.0
7	7.35087014087575E-5	0.0	0.0	0.0	0.0
8	7.35087014087575E-5	0.0	0.0	0.0	0.0
9	7.35087014087575E-5	0.0	0.0	0.0	0.0
10-11	1.7152030328710085E-4	0.0	0.0	0.0	0.0
12-13	2.0827465399147962E-4	0.0	0.0	0.0	0.0
14-15	2.4502900469585835E-4	0.0	0.0	0.0	0.0
16-17	3.1241198098721937E-4	0.0	0.0	0.0	0.0
18-19	3.4916633169159816E-4	0.0	0.0	1.8377175352189377E-5	0.0
20-21	3.6754350704378756E-4	0.0	0.0	2.4502900469585835E-5	0.0
22-23	4.533036586873379E-4	0.0	0.0	3.675435070437875E-5	0.0
24-25	5.819438861526636E-4	0.0	0.0	7.963442652615397E-5	0.0
26-27	6.615783126788176E-4	0.0	0.0	2.0214892887408315E-4	0.0
28-29	6.738297629136105E-4	0.0	0.0	7.718413647919539E-4	0.0
30-31	7.105841136179893E-4	0.0	0.0	0.001960232037566867	0.0
32-33	7.963442652615397E-4	0.0	0.0	0.003975595601190302	0.0
34-35	8.759786917876937E-4	0.0	0.0	0.006517771524909833	0.0
36-37	9.556131183138476E-4	0.0	0.0	0.009801160187834335	0.0
38-39	9.678645685486405E-4	0.0	0.0	0.016153537134574462	0.0
40-41	0.001016870369487812	0.0	0.0	0.02292246338929755	0.0
42-43	0.001016870369487812	0.0	0.0	0.028797033776880754	0.0
44-45	0.0010720018955443803	0.0	0.0	0.03486762736822065	0.0
46-47	0.001133259146718345	0.0	0.0	0.04103623256143887	0.0
48-49	0.0011700134974227238	0.0	0.0	0.0477622787403402	0.0
50-51	0.00117613922254012	0.0	0.0	0.054659845222528605	0.0
52-53	0.0012435221988314812	0.0	0.0	0.061980086737817366	0.0
54-55	0.0012496479239488777	0.0	0.0	0.06845497818690544	0.0
56-57	0.0012496479239488777	0.0	0.0	0.07348419850828791	0.0
58-59	0.0012496479239488777	0.0	0.0	0.07878295073483585	0.0
60-61	0.0012741508244184636	6.125725117396459E-6	0.0	0.08242163145456935	0.0
62-63	0.0012741508244184636	1.2251450234792918E-5	0.0	0.08527621935927611	0.0
64-65	0.0012741508244184636	1.2251450234792918E-5	0.0	0.08941108381351871	0.0
66-67	0.0012802765495358599	1.2251450234792918E-5	0.0	0.09536528862762808	0.0
68-69	0.001359910976062014	1.2251450234792918E-5	0.0	0.10192594022835968	0.0
70-71	0.001372162426296807	1.2251450234792918E-5	0.0	0.10761061313730359	0.0
72-73	0.0013844138765315998	1.2251450234792918E-5	0.0	0.11224166132605531	0.0
74-75	0.0013844138765315998	1.2251450234792918E-5	0.0	0.11571494746761911	0.0
76-77	0.0014089167770011855	1.2251450234792918E-5	0.0	0.11893095315425226	0.0
78-79	0.0014334196774707713	1.2251450234792918E-5	0.0	0.12107495694534101	0.0
80-81	0.0014334196774707713	1.2251450234792918E-5	0.0	0.12174878670825462	0.0
82-83	0.0014579225779403572	1.2251450234792918E-5	0.0	0.12228785051858551	0.0
84-85	0.001482425478409943	1.2251450234792918E-5	0.0	0.12256963387398576	0.0
86-87	0.001482425478409943	1.2251450234792918E-5	0.0	0.12275953135262505	0.0
88	0.001482425478409943	1.2251450234792918E-5	0.0	0.12279628570332941	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21680	0.0	48.357204	1
GTATCAA	39295	0.0	35.873566	1
TATCAAC	49260	0.0	28.438923	2
ATCAACG	49320	0.0	28.361908	3
AACGCAG	49575	0.0	28.271475	6
TCAACGC	49755	0.0	28.130693	4
CAACGCA	49745	0.0	28.087296	5
ACGCAGA	54850	0.0	25.485748	7
CGCAGAG	55260	0.0	25.289112	8
GCAGAGT	61105	0.0	22.911007	9
GTACATG	33955	0.0	21.451	1
GAGTACT	42590	0.0	21.218508	12-13
TACATGG	34490	0.0	20.731653	2
ACATGGG	33715	0.0	20.713715	3
ACGGTAC	745	0.0	20.69439	3
CAGAGTA	60225	0.0	20.225006	10-11
GTACTTT	46775	0.0	19.250889	14-15
GTGGTAT	10260	0.0	19.087996	1
AGAGTAC	58475	0.0	19.076906	10-11
AGTACTT	44740	0.0	18.638638	12-13
>>END_MODULE