FastQCFastQC Report
Fri 27 May 2016
SRR2138761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345306
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257150.045.8330881
GTATCAA459500.034.8170661
TATCAAC577600.027.4594762
ATCAACG585350.027.1442533
TCAACGC589200.027.0012914
CAACGCA593750.026.753695
AACGCAG597500.026.657636
ACGCAGA662950.023.9904297
CGCAGAG668200.023.8201478
GCAGAGT730250.021.7405959
GTGGTAT123350.021.3317321
ACATGGG437950.020.3479843
TGGTATC125950.020.3119562
TACATGG447300.020.2309322
GAGTACT482050.020.20061112-13
GTACATG456300.020.0895731
CAGAGTA726850.018.95230110-11
TGCGCGA5358.0035534E-1118.70671894
GTACTTT528650.018.37987514-15
CATGGGG267650.018.3137514
Produced by FastQC (version 0.11.5)