##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9345306
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69202902505279	33.0	33.0	33.0	33.0	33.0
2	32.622793410938066	33.0	33.0	33.0	33.0	33.0
3	32.69557829353047	33.0	33.0	33.0	33.0	33.0
4	36.670888144272645	37.0	37.0	37.0	37.0	37.0
5	36.67428214763647	37.0	37.0	37.0	37.0	37.0
6	36.66987073510487	37.0	37.0	37.0	37.0	37.0
7	36.65438595590128	37.0	37.0	37.0	37.0	37.0
8	36.64974790552605	37.0	37.0	37.0	37.0	37.0
9	36.64774476084571	37.0	37.0	37.0	37.0	37.0
10-11	36.650965308145075	37.0	37.0	37.0	37.0	37.0
12-13	36.64911421841083	37.0	37.0	37.0	37.0	37.0
14-15	38.82364964828332	40.0	37.0	40.0	37.0	40.0
16-17	38.75110499324474	40.0	37.0	40.0	37.0	40.0
18-19	38.80819467013707	40.0	37.0	40.0	37.0	40.0
20-21	38.831497571457376	40.0	37.0	40.0	37.0	40.0
22-23	38.83888820455009	40.0	37.0	40.0	37.0	40.0
24-25	38.82938759469066	40.0	37.0	40.0	37.0	40.0
26-27	38.74484935426503	40.0	37.0	40.0	37.0	40.0
28-29	38.63649742085356	40.0	37.0	40.0	37.0	40.0
30-31	38.46844411322487	40.0	37.0	40.0	37.0	40.0
32-33	38.3533183221286	40.0	37.0	40.0	37.0	40.0
34-35	38.320266638817344	40.0	37.0	40.0	37.0	40.0
36-37	38.294688128251174	40.0	37.0	40.0	37.0	40.0
38-39	38.24624406588315	40.0	37.0	40.0	37.0	40.0
40-41	38.22496971326381	40.0	37.0	40.0	37.0	40.0
42-43	38.192665290752146	40.0	37.0	40.0	37.0	40.0
44-45	38.09444816284247	40.0	37.0	40.0	37.0	40.0
46-47	37.71656472030823	40.0	37.0	40.0	33.0	40.0
48-49	37.88318165603023	40.0	37.0	40.0	33.0	40.0
50-51	37.89461285594227	40.0	37.0	40.0	33.0	40.0
52-53	37.85626315564509	40.0	37.0	40.0	33.0	40.0
54-55	37.75443202079709	40.0	37.0	40.0	33.0	40.0
56-57	37.57971366916659	40.0	37.0	40.0	33.0	40.0
58-59	37.40542799219449	38.5	37.0	40.0	33.0	40.0
60-61	37.220249042649186	37.0	37.0	40.0	33.0	40.0
62-63	37.070274923300076	37.0	37.0	40.0	33.0	40.0
64-65	36.89143948943374	37.0	37.0	40.0	33.0	40.0
66-67	36.681431168458744	37.0	37.0	40.0	33.0	40.0
68-69	36.476955059232004	37.0	37.0	40.0	33.0	40.0
70-71	36.26117420214879	37.0	37.0	38.5	33.0	40.0
72-73	35.985427852279436	37.0	37.0	37.0	33.0	40.0
74-75	35.65639450863731	37.0	35.0	37.0	33.0	40.0
76-77	33.463123666002446	35.0	33.0	37.0	27.0	37.0
78-79	35.17629086792179	37.0	33.0	37.0	33.0	37.0
80-81	35.40022871698481	37.0	33.0	37.0	33.0	37.0
82-83	35.23500740227042	37.0	33.0	37.0	33.0	37.0
84-85	35.09215202787876	37.0	33.0	37.0	33.0	37.0
86-87	35.00249200288329	37.0	33.0	37.0	33.0	37.0
88-89	34.83137826488924	37.0	33.0	37.0	33.0	37.0
90-91	34.77578625166275	37.0	33.0	37.0	33.0	37.0
92-93	34.82440382996484	37.0	33.0	37.0	33.0	37.0
94-95	34.80998385460736	37.0	33.0	37.0	33.0	37.0
96-97	34.80611776364057	37.0	33.0	37.0	33.0	37.0
98-99	34.81467353082242	37.0	33.0	37.0	33.0	37.0
100	34.77049359471361	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	6.0
18	8.0
19	20.0
20	66.0
21	228.0
22	812.0
23	2243.0
24	5346.0
25	10969.0
26	19908.0
27	33240.0
28	50405.0
29	71933.0
30	99660.0
31	135564.0
32	184514.0
33	255609.0
34	365234.0
35	566535.0
36	1110250.0
37	2988172.0
38	3319002.0
39	125581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61776367919506	20.15125242033299	14.810977276825103	22.420006623646845
2	14.90300965409202	20.806772631259054	42.379259646066565	21.91095806858236
3	17.843021940640575	27.109791803500066	31.18163278976633	23.865553466093033
4	12.127831876238188	18.073180268254458	39.22533943778834	30.573648417719014
5	12.642560874946204	38.05386361880499	35.51300513862253	13.79057036762627
6	28.631571828680624	38.3903855047657	18.726952333075022	14.251090333478647
7	25.403427132295082	33.99993536862249	23.22484678404324	17.371790715039186
8	24.455828412681193	36.0401895882275	21.20038658980241	18.303595409288896
9	25.913982912919064	16.575861721381834	21.651190447910427	35.85896491778867
10-11	24.059256464392398	26.946671782319314	28.615094346285346	20.378977407002942
12-13	25.180097284343827	24.855188315519666	28.99912873542713	20.965585664709376
14-15	22.269137040563468	25.926518618009943	27.816970359237033	23.98737398218956
16-17	20.63714125572774	28.950876514904916	28.412975455271344	21.999006774096
18-19	20.39956744059531	27.405105836020777	31.49597776680614	20.699348956577772
20-21	21.651386101595016	26.52961170835355	31.52142228998524	20.297579900066197
22-23	21.304261347780713	26.443669879765768	31.410666214749078	20.84140255770444
24-25	20.82392632444735	26.76693827612339	31.63453114143893	20.774604257990323
26-27	20.69357127415875	27.033078944720558	31.492075776059124	20.78127400506157
28-29	20.560172530652356	27.233471418245593	31.433420014960017	20.77293603614203
30-31	21.010634083076724	27.125037114347215	31.17934474502477	20.684984057551294
32-33	20.633059068178756	27.41559749064022	30.99055120344023	20.96079223774079
34-35	20.762253486426776	27.503880434261635	31.017047885762445	20.71681819354914
36-37	21.014356678297474	27.23355571366258	30.845944298189988	20.906143309849963
38-39	20.741737942717613	27.509549889537094	30.8386122076622	20.91009996008309
40-41	21.070183179760548	27.598401732472517	30.577646406253905	20.75376868151303
42-43	21.2222331231659	27.671889974881292	29.972477688785183	21.13339921316763
44-45	21.19760457767169	28.12048260984224	29.495224753644827	21.18668805884124
46-47	21.450101588253446	27.95522650126322	28.807506155670655	21.787165754812683
48-49	21.547406614605165	28.10632220972426	28.568050700212634	21.77822047545794
50-51	21.422218164705995	28.305325960532713	28.344372213314706	21.928083661446585
52-53	21.72442539088837	28.280212656444498	28.107581315114526	21.88778063755261
54-55	21.748983842462476	28.234861322721024	28.100407642379377	21.91574719243713
56-57	21.80109040762441	28.381445104352498	27.83828034358763	21.97918414443546
58-59	21.689101468823008	28.365920326276196	28.088926848792404	21.856051356108395
60-61	21.868766986394586	28.348108780157855	27.911836028066517	21.871288205381045
62-63	21.778047299364943	28.31021594240643	27.92518428620772	21.9865524720209
64-65	21.684429833786034	28.36470597887642	27.881246307163032	22.069617880174512
66-67	21.95902786016302	28.396157184310493	27.51707238474713	22.12774257077935
68-69	21.880105476603937	28.13877073506016	27.78912253786216	22.192001250473744
70-71	21.902670403314904	27.7476333601833	27.84639924373165	22.503296992770146
72-73	22.133252921803898	27.873749513700723	27.79509887068941	22.19789869380597
74-75	21.867029625610115	28.0756537632588	27.72831926323868	22.3289973478924
76-77	22.01397108711942	27.892570666817345	27.642915284436214	22.450542961627022
78-79	22.16418941322432	27.9004489779448	27.489716748983206	22.445644859847672
80-81	22.156351054204386	27.75962263070281	27.59056732568372	22.493458989409085
82-83	22.373942487593823	27.581578222213864	27.713322653817112	22.3311566363752
84-85	22.621100888686215	27.51154793139563	27.509869960202384	22.357481219715776
86-87	22.162247271609598	27.725268889525474	27.59949551833816	22.512988320526773
88-89	22.42148872085757	27.507551399796082	27.511394117144896	22.55956576220145
90-91	22.45799374954049	27.42529208589925	27.757943845142453	22.358770319417808
92-93	22.572938288234134	27.502184884926745	27.544909228052884	22.379967598786237
94-95	22.356196353287135	27.32487179221331	27.658302758298024	22.660629096201525
96-97	22.473940498756043	27.415281150385212	27.78264548707851	22.328132863780233
98-99	23.478991020909312	29.441782644452662	27.886259276851742	19.19296705778628
100	22.704643407979262	30.02538862411474	21.65495629276262	25.615011675143386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2976.0
1	3078.5
2	5549.0
3	8442.0
4	12181.0
5	21536.5
6	28767.5
7	32194.5
8	33172.0
9	30835.0
10	27131.5
11	23624.5
12	25913.5
13	36055.0
14	59906.0
15	93080.0
16	119792.0
17	134859.5
18	137105.0
19	127138.0
20	110047.0
21	89866.5
22	73795.5
23	67801.0
24	70842.5
25	81504.5
26	99092.5
27	117277.0
28	131545.5
29	148607.5
30	165892.5
31	181802.0
32	204760.0
33	230110.5
34	254585.5
35	277989.5
36	307370.0
37	331358.0
38	335343.0
39	338574.5
40	349668.0
41	358655.5
42	362697.0
43	372720.5
44	386889.0
45	400111.0
46	407757.0
47	411218.5
48	417777.0
49	414741.0
50	399181.0
51	378245.0
52	357629.0
53	339353.0
54	313843.5
55	278714.5
56	244473.5
57	210013.0
58	175949.0
59	149612.5
60	122155.5
61	94115.5
62	71263.5
63	51282.0
64	35323.0
65	24808.0
66	17818.0
67	13401.5
68	10493.5
69	7771.5
70	5652.0
71	4176.0
72	3294.5
73	2616.0
74	1939.5
75	1310.0
76	877.5
77	575.0
78	377.5
79	283.0
80	205.5
81	156.0
82	127.5
83	90.5
84	57.5
85	42.0
86	29.5
87	20.0
88	15.0
89	11.5
90	8.5
91	7.5
92	6.5
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0700559189822142E-5
2	4.922257227318185E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0014071235334616116
12-13	0.009015221117425155
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.354910624399715E-6
28-29	1.0720183538118318E-5
30-31	1.0744155810458264E-5
32-33	5.393363918740559E-6
34-35	0.0059094919560971374
36-37	0.0470603804334949
38-39	0.1538661063932646
40-41	0.1821395371852526
42-43	0.055323339701779786
44-45	0.005122765293918372
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.01502798261851302
64-65	0.10542070027523083
66-67	0.22824890980731688
68-69	0.25187430673160566
70-71	0.11648712124691409
72-73	0.007776072674969685
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	168.0
20-21	855.0
22-23	1970.0
24-25	3646.0
26-27	7316.0
28-29	15599.0
30-31	34750.0
32-33	44921.0
34-35	59252.0
36-37	81090.0
38-39	98064.0
40-41	105242.0
42-43	128802.0
44-45	155254.0
46-47	142156.0
48-49	116730.0
50-51	96353.0
52-53	89127.0
54-55	96454.0
56-57	97290.0
58-59	93441.0
60-61	88017.0
62-63	81641.0
64-65	82900.0
66-67	107976.0
68-69	99282.0
70-71	96440.0
72-73	92171.0
74-75	91976.0
76-77	87640.0
78-79	93047.0
80-81	96955.0
82-83	98271.0
84-85	106654.0
86-87	107251.0
88-89	111395.0
90-91	114103.0
92-93	116956.0
94-95	124776.0
96-97	274496.0
98-99	506443.0
100-101	5298436.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.49614292159043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23250963502986	67.60183034085287
2	7.62812854676657	11.823010801689723
3	2.373028894245985	5.5170175903665175
4	1.0370403478987125	3.2146650806485795
5	0.5630143119543584	2.1815718793057925
6	0.3354073183144698	1.5595664086227317
7	0.21121093847622152	1.1457623152329555
8	0.14640650817928577	0.9076751746010343
9	0.0991611803769557	0.6916148106089551
>10	0.3640269173127656	4.29627347923303
>50	0.007354703540228785	0.37016804727459246
>100	0.002341514138535032	0.33171306625971053
>500	1.5382658085223613E-4	0.08769062892625273
>1k	2.1535718521450297E-4	0.27144037637724316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	1.0700559189822142E-5	0.0	0.0	1.0700559189822142E-5	0.0
12-13	1.0700559189822142E-5	0.0	0.0	1.0700559189822142E-5	5.350279594911071E-6
14-15	1.0700559189822142E-5	1.0700559189822142E-5	0.0	1.0700559189822142E-5	1.0700559189822142E-5
16-17	1.0700559189822142E-5	1.0700559189822142E-5	0.0	1.6050838784733213E-5	2.1401118379644284E-5
18-19	1.0700559189822142E-5	1.0700559189822142E-5	0.0	3.2101677569466425E-5	2.1401118379644284E-5
20-21	1.0700559189822142E-5	1.0700559189822142E-5	0.0	4.815251635419964E-5	2.1401118379644284E-5
22-23	1.0700559189822142E-5	1.0700559189822142E-5	0.0	5.8853075544021776E-5	2.1401118379644284E-5
24-25	1.0700559189822142E-5	1.0700559189822142E-5	0.0	8.025419392366606E-5	2.1401118379644284E-5
26-27	1.0700559189822142E-5	1.0700559189822142E-5	0.0	1.979603450117096E-4	2.1401118379644284E-5
28-29	1.0700559189822142E-5	1.0700559189822142E-5	0.0	5.082765615165518E-4	2.1401118379644284E-5
30-31	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.001492728006980189	2.1401118379644284E-5
32-33	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.0033439247468194195	2.1401118379644284E-5
34-35	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.005473336025594026	3.2101677569466425E-5
36-37	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.008469492598744225	3.2101677569466425E-5
38-39	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.013209840319835435	3.2101677569466425E-5
40-41	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.01837821040851953	3.2101677569466425E-5
42-43	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.023460976023685045	3.2101677569466425E-5
44-45	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.030282582507196662	4.280223675928857E-5
46-47	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.0375001096807317	5.350279594911071E-5
48-49	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.04469088545629217	5.350279594911071E-5
50-51	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.05143223774588013	5.350279594911071E-5
52-53	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.05832339786412558	6.420335513893285E-5
54-55	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.06464742834531047	6.420335513893285E-5
56-57	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.071715147690188	6.420335513893285E-5
58-59	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.07851535305531997	6.420335513893285E-5
60-61	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.08465212375068296	6.420335513893285E-5
62-63	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.08975094020463321	7.490391432875499E-5
64-65	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.09458759295843283	9.095475311348821E-5
66-67	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.10072436365379582	9.630503270839927E-5
68-69	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.10794724110692577	9.630503270839927E-5
70-71	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.11355968440198748	9.630503270839927E-5
72-73	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.11809137121887715	1.0165531230331034E-4
74-75	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.12181516581693526	1.0700559189822142E-4
76-77	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.1254159039843104	1.0700559189822142E-4
78-79	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.12762556945700868	1.0700559189822142E-4
80-81	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.1283532074819166	1.0700559189822142E-4
82-83	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.12911829746398887	1.1770615108804355E-4
84-85	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.1295142181540123	1.1770615108804355E-4
86-87	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.12977103157456804	1.2305643068295463E-4
88	1.0700559189822142E-5	1.0700559189822142E-5	0.0	0.12979778297254257	1.284067102778657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25715	0.0	45.833088	1
GTATCAA	45950	0.0	34.817066	1
TATCAAC	57760	0.0	27.459476	2
ATCAACG	58535	0.0	27.144253	3
TCAACGC	58920	0.0	27.001291	4
CAACGCA	59375	0.0	26.75369	5
AACGCAG	59750	0.0	26.65763	6
ACGCAGA	66295	0.0	23.990429	7
CGCAGAG	66820	0.0	23.820147	8
GCAGAGT	73025	0.0	21.740595	9
GTGGTAT	12335	0.0	21.331732	1
ACATGGG	43795	0.0	20.347984	3
TGGTATC	12595	0.0	20.311956	2
TACATGG	44730	0.0	20.230932	2
GAGTACT	48205	0.0	20.200611	12-13
GTACATG	45630	0.0	20.089573	1
CAGAGTA	72685	0.0	18.952301	10-11
TGCGCGA	535	8.0035534E-11	18.706718	94
GTACTTT	52865	0.0	18.379875	14-15
CATGGGG	26765	0.0	18.313751	4
>>END_MODULE