FastQCFastQC Report
Fri 27 May 2016
SRR2138760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9511762
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC135950.045.5525471
GGTATCA269650.043.366251
TATACAC163350.039.620483
TTATACA184200.034.516892
GTATCAA465000.032.8003231
TACACAT252050.025.990015
ATACACA257650.025.882414
TATCAAC589800.025.7932012
ATCAACG589600.025.6951143
TCAACGC594550.025.4811884
ACACATC247100.025.4475756
CAACGCA596700.025.383325
AACGCAG598650.025.3759546
ACATCTC267050.023.6799168
CACATCT266750.023.677067
ACGCAGA657150.023.063967
CGCAGAG659800.022.9772858
CATCTCC295550.021.2766959
GCAGAGT724300.020.8985469
GAGTACT490450.019.89222112-13
Produced by FastQC (version 0.11.5)