##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9511762
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67329617793212	33.0	33.0	33.0	33.0	33.0
2	32.607737136400175	33.0	33.0	33.0	33.0	33.0
3	32.680461832413386	33.0	33.0	33.0	33.0	33.0
4	36.64976741428139	37.0	37.0	37.0	37.0	37.0
5	36.65567809623496	37.0	37.0	37.0	37.0	37.0
6	36.65009669081291	37.0	37.0	37.0	37.0	37.0
7	36.63411185014932	37.0	37.0	37.0	37.0	37.0
8	36.62923525630688	37.0	37.0	37.0	37.0	37.0
9	36.629477272454885	37.0	37.0	37.0	37.0	37.0
10-11	36.63230193312238	37.0	37.0	37.0	37.0	37.0
12-13	36.631134904342645	37.0	37.0	37.0	37.0	37.0
14-15	38.77876853941467	40.0	37.0	40.0	37.0	40.0
16-17	38.69852977818411	40.0	37.0	40.0	37.0	40.0
18-19	38.758051347373915	40.0	37.0	40.0	37.0	40.0
20-21	38.78459537407285	40.0	37.0	40.0	37.0	40.0
22-23	38.79093259347906	40.0	37.0	40.0	37.0	40.0
24-25	38.787510766093376	40.0	37.0	40.0	37.0	40.0
26-27	38.69591720941105	40.0	37.0	40.0	37.0	40.0
28-29	38.568515820215936	40.0	37.0	40.0	37.0	40.0
30-31	38.38556079152727	40.0	37.0	40.0	37.0	40.0
32-33	38.26664442473878	40.0	37.0	40.0	37.0	40.0
34-35	38.21247058001302	40.0	37.0	40.0	37.0	40.0
36-37	38.17320016580586	40.0	37.0	40.0	37.0	40.0
38-39	38.1190489965932	40.0	37.0	40.0	37.0	40.0
40-41	38.12009878237664	40.0	37.0	40.0	37.0	40.0
42-43	38.1067035938859	40.0	37.0	40.0	37.0	40.0
44-45	38.006950108438446	40.0	37.0	40.0	33.0	40.0
46-47	37.618234034426635	40.0	37.0	40.0	33.0	40.0
48-49	37.81097379533992	40.0	37.0	40.0	33.0	40.0
50-51	37.7915843370145	40.0	37.0	40.0	33.0	40.0
52-53	37.784760293179346	40.0	37.0	40.0	33.0	40.0
54-55	37.70520775089419	40.0	37.0	40.0	33.0	40.0
56-57	37.500319410798554	40.0	37.0	40.0	33.0	40.0
58-59	37.34467445512401	38.5	37.0	40.0	33.0	40.0
60-61	37.17012037761728	37.0	37.0	40.0	33.0	40.0
62-63	37.06625182265813	37.0	37.0	40.0	33.0	40.0
64-65	36.92909941215778	37.0	37.0	40.0	33.0	40.0
66-67	36.753002087837416	37.0	37.0	40.0	33.0	40.0
68-69	36.575354611902085	37.0	37.0	40.0	33.0	40.0
70-71	36.37072528201813	37.0	37.0	40.0	33.0	40.0
72-73	36.1023440224969	37.0	37.0	37.0	33.0	40.0
74-75	35.77478430791061	37.0	37.0	37.0	33.0	40.0
76-77	33.60022990706514	35.0	33.0	37.0	27.0	37.0
78-79	35.27405363289573	37.0	33.0	37.0	33.0	37.0
80-81	35.48121417369512	37.0	33.0	37.0	33.0	37.0
82-83	35.31088204080474	37.0	33.0	37.0	33.0	37.0
84-85	35.157132971452455	37.0	33.0	37.0	33.0	37.0
86-87	35.06143694545099	37.0	33.0	37.0	33.0	37.0
88-89	34.891012159423944	37.0	33.0	37.0	33.0	37.0
90-91	34.83606911305692	37.0	33.0	37.0	33.0	37.0
92-93	34.884902880694895	37.0	33.0	37.0	33.0	37.0
94-95	34.87437979465156	37.0	33.0	37.0	33.0	37.0
96-97	34.87776390599619	37.0	33.0	37.0	33.0	37.0
98-99	34.88688493480532	37.0	33.0	37.0	33.0	37.0
100	34.73643984563663	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	6.0
19	23.0
20	77.0
21	245.0
22	993.0
23	2799.0
24	6334.0
25	12325.0
26	21655.0
27	34966.0
28	52315.0
29	74827.0
30	104064.0
31	141936.0
32	192356.0
33	265341.0
34	377960.0
35	579411.0
36	1106694.0
37	2878226.0
38	3505212.0
39	153996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.136881201468626	21.125798077937006	14.737126960428665	23.000193760165708
2	14.402472486314515	20.895419451432605	43.3705319994142	21.331576062838682
3	17.388821837388665	27.79130255599384	31.944308939670474	22.875566666947023
4	11.722509819423536	18.683555934968922	39.00317081171097	30.590763433896566
5	12.058072106731867	38.24775454303362	36.21910811257768	13.475065237656834
6	27.763313228749126	38.742626102569226	18.891359864908296	14.602700803773349
7	24.363669356284287	34.02743193400254	23.52417181213868	18.084726897574487
8	23.493722824435682	36.696229363182134	21.96595120861939	17.844096603762793
9	25.009099260473505	16.577296614444307	22.025961120557895	36.38764300452429
10-11	23.554606702259704	27.160273173866262	28.97091004056311	20.314210083310922
12-13	24.734512406681837	24.78552206729017	29.246930128435732	21.233035397592257
14-15	21.765315406335862	26.134059073387245	27.698653519715904	24.40197200056099
16-17	20.39987438710094	29.58166425947159	28.249014220498793	21.769447132928686
18-19	20.20992535347289	27.54149020970037	31.341679911671466	20.906904525155277
20-21	21.21194630291056	26.806463761277488	31.398933998133614	20.582655937678336
22-23	20.998845937340356	27.154146463384944	31.11163872533987	20.735368873934835
24-25	20.630789820113378	27.567543661429955	31.454056837228766	20.347609681227897
26-27	20.541541827635005	27.90010295800105	31.458725669542698	20.099629544821244
28-29	20.12493668660024	27.632751154651547	31.63969868272841	20.602613476019805
30-31	20.479955644875396	28.082053179374338	31.16024054271464	20.277750633035623
32-33	20.992979040538703	27.673388688875526	31.157600479643143	20.176031790942623
34-35	20.397817077944	27.808529496253026	31.262722365283253	20.530931060519723
36-37	20.25707985787175	28.247403109563958	30.96125760061884	20.53425943194545
38-39	20.397608410433477	27.618303856408765	31.131307792426792	20.85277994073097
40-41	20.546270449495466	27.64129269041518	31.179368001636863	20.63306885845249
42-43	20.904196239738663	27.592724695970517	30.773536840216977	20.729542224073843
44-45	20.575524809729657	28.368934378113043	30.06252653101351	20.993014281143786
46-47	21.355169594364508	27.86691317182714	29.062585266810625	21.715331966997727
48-49	21.24116262191254	28.006339407812725	28.94685201629207	21.80564595398267
50-51	20.860847561309058	28.278298288761615	29.095263243231557	21.76559090669777
52-53	21.067556337431604	28.482767436373972	29.153208604941305	21.296467621253115
54-55	21.030654517367672	28.5106621396685	28.62830908984002	21.830374253123804
56-57	21.650182791078976	28.926700752321967	27.92352560450603	21.499590852093032
58-59	20.881153043805188	29.36941543086069	28.692105811347542	21.057325713986582
60-61	21.33201910042605	29.71604741159155	27.77203807374327	21.179895414239134
62-63	21.081308087466326	29.965079797669684	27.641190406220296	21.312421708643694
64-65	21.16400463834586	29.68030379051948	27.747757773904315	21.407933797230342
66-67	21.362790480300482	29.30708352817803	27.671050865875703	21.65907512564579
68-69	21.409955094000562	28.87414558016464	27.971793451785366	21.74410587404943
70-71	21.561109609709142	28.292978197943704	28.062442174777917	22.083470017569237
72-73	21.597794108613524	28.3513499474485	28.20635105494921	21.84450488898877
74-75	21.50862096628803	28.5253500517223	28.051805146091425	21.914223835898248
76-77	21.61705838760569	28.421497998517857	27.808393713413814	22.153049900462634
78-79	21.74565773083823	28.323920474493452	27.94551994209506	21.98490185257326
80-81	21.561910451827657	28.2995569430706	28.08305297700017	22.05547962810158
82-83	21.98147835008521	27.91520583581667	28.227198098557555	21.876117715540566
84-85	22.205557698074667	27.928057186445304	27.861805520299466	22.004579595180562
86-87	21.748477889522043	28.093065323648858	27.98961838995963	22.168838396869468
88-89	22.060581994805165	27.75905650339932	27.945827495461618	22.2345340063339
90-91	22.123275682392098	27.707709619418313	28.10534510808936	22.06366959010023
92-93	22.210701143006354	27.733526553503374	28.013559489534117	22.04221281395615
94-95	21.857343892123005	27.728981472044794	28.157430046596872	22.25624458923533
96-97	22.03849014573165	27.763053176367876	28.25231156824643	21.946145109654047
98-99	23.179452229247506	29.782575364175457	28.23738709741823	18.8005853091588
100	29.785630969933923	40.64825635200561	29.566112678060463	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2805.0
1	2852.5
2	5472.5
3	8435.5
4	12499.5
5	23424.0
6	31871.0
7	35139.5
8	34985.5
9	31265.5
10	26940.0
11	23072.0
12	24936.0
13	34863.0
14	58815.5
15	92719.0
16	120694.5
17	137453.5
18	141178.0
19	132204.5
20	116316.0
21	98132.0
22	83718.5
23	79300.0
24	85169.5
25	98078.0
26	117187.5
27	136995.0
28	154470.5
29	176648.0
30	197308.5
31	213926.0
32	234973.5
33	258451.5
34	281411.5
35	305719.0
36	331503.0
37	353400.0
38	364465.0
39	368917.0
40	375277.0
41	380999.5
42	388800.5
43	402842.5
44	420270.0
45	442114.5
46	462240.5
47	472501.0
48	464489.5
49	443705.0
50	423254.5
51	396963.0
52	364199.0
53	333855.0
54	300548.5
55	263879.0
56	226049.5
57	185683.5
58	151419.5
59	122235.5
60	94958.0
61	70494.5
62	51326.0
63	37779.0
64	26348.5
65	17870.0
66	12824.0
67	9861.0
68	8069.0
69	6217.5
70	4541.5
71	3305.0
72	2239.5
73	1524.5
74	1106.5
75	779.0
76	529.0
77	324.5
78	189.5
79	126.5
80	99.0
81	78.5
82	64.0
83	46.0
84	28.5
85	16.5
86	11.5
87	7.5
88	3.5
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.153989765513477E-5
2	4.3104526795350847E-4
3	2.1026598436756514E-5
4	2.1026598436756514E-5
5	1.0513299218378257E-5
6	1.0513299218378257E-5
7	1.0513299218378257E-5
8	0.0
9	0.0
10-11	0.0017241810718140339
12-13	0.008605135410242604
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.25683392352684E-6
22-23	5.2574488586919715E-6
24-25	0.0
26-27	0.0
28-29	2.106715571961954E-5
30-31	2.1117461072996146E-5
32-33	1.5910318414263115E-5
34-35	0.00576728190564652
36-37	0.046969100054724676
38-39	0.15278636893682462
40-41	0.18120278595565495
42-43	0.055651749625883845
44-45	0.004841409186471648
46-47	5.794304060045678E-6
48-49	1.1796440388723383E-5
50-51	1.1979308141047968E-5
52-53	6.072561277608597E-6
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.015390758151766928
64-65	0.10660190202486158
66-67	0.23191483835731408
68-69	0.25716281795203566
70-71	0.11933926325423763
72-73	0.0076940771697980095
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	190.0
20-21	862.0
22-23	2020.0
24-25	4214.0
26-27	7719.0
28-29	16445.0
30-31	40334.0
32-33	52236.0
34-35	69316.0
36-37	95726.0
38-39	116097.0
40-41	120895.0
42-43	144695.0
44-45	170877.0
46-47	157821.0
48-49	135026.0
50-51	115369.0
52-53	111458.0
54-55	121452.0
56-57	126563.0
58-59	129430.0
60-61	126411.0
62-63	129019.0
64-65	140861.0
66-67	154318.0
68-69	124832.0
70-71	111671.0
72-73	104720.0
74-75	102905.0
76-77	98929.0
78-79	103622.0
80-81	107948.0
82-83	99671.0
84-85	108739.0
86-87	116749.0
88-89	122176.0
90-91	121205.0
92-93	122594.0
94-95	125927.0
96-97	263490.0
98-99	1718743.0
100-101	3668487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.62030449289415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98972471865277	69.17778949626414
2	7.225583356748089	11.361551272926459
3	2.1535795128535753	5.079452311506202
4	0.9742176863643364	3.0637316457730788
5	0.5285362018629716	2.0776838562992297
6	0.32950456477194523	1.5543449528501336
7	0.21190409978375174	1.166197539380383
8	0.13655011636130454	0.8588489381492712
9	0.10335140581928808	0.731296709575299
>10	0.34128864052342794	4.0171661216155075
>50	0.004017874996223734	0.2127874950066908
>100	0.0013167171416687414	0.19841929412131531
>500	9.109378848075659E-5	0.046145997335119246
>1k	3.3401033217503686E-4	0.4545843691971573
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0513299218378257E-5	0.0
12-13	0.0	0.0	0.0	1.0513299218378257E-5	0.0
14-15	0.0	0.0	0.0	1.0513299218378257E-5	0.0
16-17	0.0	0.0	0.0	2.1026598436756514E-5	0.0
18-19	0.0	0.0	0.0	5.2566496091891285E-5	0.0
20-21	0.0	0.0	0.0	6.307979531026954E-5	0.0
22-23	0.0	0.0	0.0	9.461969296540431E-5	0.0
24-25	0.0	0.0	0.0	1.7346943710324123E-4	0.0
26-27	0.0	0.0	0.0	3.25912275769726E-4	0.0
28-29	0.0	0.0	0.0	0.0010197900241826908	5.2566496091891285E-6
30-31	0.0	0.0	0.0	0.0028543607377896968	1.0513299218378257E-5
32-33	0.0	0.0	0.0	0.007085963673186944	1.0513299218378257E-5
34-35	0.0	0.0	0.0	0.012200683742927966	1.0513299218378257E-5
36-37	0.0	0.0	0.0	0.019354983861034368	1.0513299218378257E-5
38-39	0.0	0.0	0.0	0.030414974638768296	1.0513299218378257E-5
40-41	0.0	0.0	0.0	0.04227923280670816	1.0513299218378257E-5
42-43	0.0	0.0	0.0	0.05315524084812047	1.0513299218378257E-5
44-45	0.0	0.0	0.0	0.06547157088245059	1.0513299218378257E-5
46-47	0.0	0.0	0.0	0.07805073339724017	1.0513299218378257E-5
48-49	0.0	0.0	0.0	0.09018833734485787	1.0513299218378257E-5
50-51	0.0	0.0	0.0	0.10233645459169395	1.0513299218378257E-5
52-53	0.0	0.0	0.0	0.11374864089324355	1.0513299218378257E-5
54-55	0.0	0.0	0.0	0.12376255839874883	1.0513299218378257E-5
56-57	0.0	0.0	0.0	0.13136367373363633	1.0513299218378257E-5
58-59	0.0	0.0	0.0	0.13892799252125948	1.0513299218378257E-5
60-61	0.0	0.0	0.0	0.14656590440341127	1.0513299218378257E-5
62-63	0.0	0.0	0.0	0.15374123111995444	1.0513299218378257E-5
64-65	0.0	0.0	0.0	0.1611057972224284	1.0513299218378257E-5
66-67	0.0	0.0	0.0	0.16963208288853315	1.0513299218378257E-5
68-69	0.0	0.0	0.0	0.1773593578140412	1.0513299218378257E-5
70-71	0.0	0.0	0.0	0.18388285997904488	1.0513299218378257E-5
72-73	0.0	0.0	0.0	0.18911848298979728	1.0513299218378257E-5
74-75	0.0	0.0	0.0	0.19381792774041234	1.0513299218378257E-5
76-77	0.0	0.0	0.0	0.19778144154574095	1.0513299218378257E-5
78-79	0.0	0.0	0.0	0.2001206506218301	1.0513299218378257E-5
80-81	0.0	0.0	0.0	0.2009354313112544	1.0513299218378257E-5
82-83	0.0	0.0	0.0	0.20146109627217335	1.0513299218378257E-5
84-85	0.0	0.0	0.0	0.2017712385991155	1.0513299218378257E-5
86-87	0.0	0.0	0.0	0.20194470803621872	1.0513299218378257E-5
88	0.0	0.0	0.0	0.20197099128426468	1.0513299218378257E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	13595	0.0	45.552547	1
GGTATCA	26965	0.0	43.36625	1
TATACAC	16335	0.0	39.62048	3
TTATACA	18420	0.0	34.51689	2
GTATCAA	46500	0.0	32.800323	1
TACACAT	25205	0.0	25.99001	5
ATACACA	25765	0.0	25.88241	4
TATCAAC	58980	0.0	25.793201	2
ATCAACG	58960	0.0	25.695114	3
TCAACGC	59455	0.0	25.481188	4
ACACATC	24710	0.0	25.447575	6
CAACGCA	59670	0.0	25.38332	5
AACGCAG	59865	0.0	25.375954	6
ACATCTC	26705	0.0	23.679916	8
CACATCT	26675	0.0	23.67706	7
ACGCAGA	65715	0.0	23.06396	7
CGCAGAG	65980	0.0	22.977285	8
CATCTCC	29555	0.0	21.276695	9
GCAGAGT	72430	0.0	20.898546	9
GAGTACT	49045	0.0	19.892221	12-13
>>END_MODULE