FastQCFastQC Report
Fri 27 May 2016
SRR2138759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907312
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226550.045.418321
GTATCAA395850.035.2887541
TATCAAC502650.027.5615432
ATCAACG500800.027.5236433
AACGCAG504350.027.3920576
CAACGCA506200.027.2391935
TCAACGC511050.027.0521454
ACGCAGA563000.024.4882627
CGCAGAG566500.024.3224338
GCAGAGT623150.021.9989979
GTACATG340700.020.7300471
ACATGGG329100.020.697853
TACATGG335850.020.6249962
GAGTACT442000.020.40437312-13
ACGGTAC9550.019.393863
CAGAGTA617450.019.35514610-11
GGTTCAC35200.018.9455456
AGAGTAC601000.018.5697610-11
AGTACTT465250.018.40706312-13
GTACTTT491400.018.30602614-15
Produced by FastQC (version 0.11.5)