##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7907312
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72182455934457	33.0	33.0	33.0	33.0	33.0
2	32.64928170280874	33.0	33.0	33.0	33.0	33.0
3	32.72139533636715	33.0	33.0	33.0	33.0	33.0
4	36.702548350185246	37.0	37.0	37.0	37.0	37.0
5	36.70926921816162	37.0	37.0	37.0	37.0	37.0
6	36.706357103399995	37.0	37.0	37.0	37.0	37.0
7	36.69215973772124	37.0	37.0	37.0	37.0	37.0
8	36.687614577494855	37.0	37.0	37.0	37.0	37.0
9	36.68013074480936	37.0	37.0	37.0	37.0	37.0
10-11	36.68906633505799	37.0	37.0	37.0	37.0	37.0
12-13	36.68650699504458	37.0	37.0	37.0	37.0	37.0
14-15	38.89858443678459	40.0	38.5	40.0	37.0	40.0
16-17	38.83400427351292	40.0	37.0	40.0	37.0	40.0
18-19	38.8923822659331	40.0	38.5	40.0	37.0	40.0
20-21	38.912676961003164	40.0	40.0	40.0	37.0	40.0
22-23	38.91832210984076	40.0	40.0	40.0	37.0	40.0
24-25	38.90993654459068	40.0	38.5	40.0	37.0	40.0
26-27	38.826222816534596	40.0	37.0	40.0	37.0	40.0
28-29	38.72129664259606	40.0	37.0	40.0	37.0	40.0
30-31	38.55308870432305	40.0	37.0	40.0	37.0	40.0
32-33	38.441799073612245	40.0	37.0	40.0	37.0	40.0
34-35	38.40907411995927	40.0	37.0	40.0	37.0	40.0
36-37	38.38017599030334	40.0	37.0	40.0	37.0	40.0
38-39	38.33524774597262	40.0	37.0	40.0	37.0	40.0
40-41	38.30575114718418	40.0	37.0	40.0	37.0	40.0
42-43	38.27256171858333	40.0	37.0	40.0	37.0	40.0
44-45	38.17435142466795	40.0	37.0	40.0	37.0	40.0
46-47	37.78758853613461	40.0	37.0	40.0	33.0	40.0
48-49	37.96657922965563	40.0	37.0	40.0	33.0	40.0
50-51	37.980330793340116	40.0	37.0	40.0	33.0	40.0
52-53	37.944641436377694	40.0	37.0	40.0	33.0	40.0
54-55	37.84967876385722	40.0	37.0	40.0	33.0	40.0
56-57	37.66541017712351	40.0	37.0	40.0	33.0	40.0
58-59	37.48903225350222	40.0	37.0	40.0	33.0	40.0
60-61	37.31040770766086	37.0	37.0	40.0	33.0	40.0
62-63	37.16378248131271	37.0	37.0	40.0	33.0	40.0
64-65	36.98195900293989	37.0	37.0	40.0	33.0	40.0
66-67	36.77434007599899	37.0	37.0	40.0	33.0	40.0
68-69	36.57134017634094	37.0	37.0	40.0	33.0	40.0
70-71	36.354809174226745	37.0	37.0	38.5	33.0	40.0
72-73	36.08326134031325	37.0	37.0	37.0	33.0	40.0
74-75	35.754279051474896	37.0	35.0	37.0	33.0	40.0
76-77	33.56693220891262	35.0	33.0	37.0	27.0	37.0
78-79	35.25736193755175	37.0	33.0	37.0	33.0	37.0
80-81	35.47464593340867	37.0	33.0	37.0	33.0	37.0
82-83	35.31112419962315	37.0	33.0	37.0	33.0	37.0
84-85	35.16366392234101	37.0	33.0	37.0	33.0	37.0
86-87	35.072599859902155	37.0	33.0	37.0	33.0	37.0
88-89	34.9069373821082	37.0	33.0	37.0	33.0	37.0
90-91	34.85170321419129	37.0	33.0	37.0	33.0	37.0
92-93	34.89878695176581	37.0	33.0	37.0	33.0	37.0
94-95	34.88840554676594	37.0	33.0	37.0	33.0	37.0
96-97	34.883414366295845	37.0	33.0	37.0	33.0	37.0
98-99	34.89041586846012	37.0	33.0	37.0	33.0	37.0
100	34.843424465654486	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	4.0
18	7.0
19	23.0
20	58.0
21	148.0
22	561.0
23	1683.0
24	4058.0
25	8363.0
26	15037.0
27	25019.0
28	38316.0
29	54828.0
30	76817.0
31	105250.0
32	144270.0
33	202358.0
34	293372.0
35	459223.0
36	919135.0
37	2559574.0
38	2877360.0
39	121845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.772149159720456	20.26522550212721	14.414929276192446	22.54769606195989
2	15.028067205487202	21.033780175233655	42.10920556535877	21.828947053920373
3	18.00775712698214	27.069259592612354	30.911122861140246	24.011860419265265
4	12.230406071142534	18.00976792484017	39.20179418813657	30.558031815880724
5	12.737443294551914	38.056031451407804	35.4027773544704	13.80374789956988
6	28.717405630663983	38.169483448794026	18.88556524096883	14.227545679573165
7	25.353182643252552	33.846272139795694	23.433237923739185	17.367307293212573
8	24.259182419914936	35.921718520999114	21.47092734812125	18.3481717109647
9	25.855085249587372	16.33781698987178	22.104543504106516	35.70255425643433
10-11	24.04615324592037	26.943501156132854	28.658259244019945	20.352086353926833
12-13	25.150523342516824	24.88322069931365	29.152556585457102	20.813699372712428
14-15	22.209260239130565	25.9292348651476	28.028216162458243	23.83328873326359
16-17	20.559698415845993	28.841033463710552	28.6580382815298	21.941229838913653
18-19	20.26711479197988	27.395694010809237	31.80333595032041	20.53385524689047
20-21	21.45271587308222	26.57683051317218	31.830235254740895	20.140218359004706
22-23	21.188558489567527	26.541452483596068	31.575175467216386	20.694813559620023
24-25	20.64558625442213	26.7272967419015	31.88112935876582	20.745987644910542
26-27	20.643359204870755	27.025550764280087	31.649265894440028	20.681824136409126
28-29	20.416953890422015	27.383684829341586	31.602213275956988	20.59714800427941
30-31	20.867859402039727	27.2364994109352	31.423203790273256	20.47243739675182
32-33	20.567489317576502	27.44015151732293	31.23991909479419	20.75244007030638
34-35	20.604664738252744	27.628688104426097	31.14822692294798	20.61842023437318
36-37	20.802941478722524	27.515778260668405	30.973659237960156	20.70762102264892
38-39	20.69267298435376	27.723821266378163	30.8572752503609	20.726230498907178
40-41	20.995213874379296	27.692643596007265	30.58220370651406	20.729938823099378
42-43	21.010045496401016	27.951179112483022	30.019164446954292	21.019610944161666
44-45	21.141480580521073	28.31324095314183	29.437123174349782	21.10815529198732
46-47	21.28915354114019	28.263910426880617	28.793047905893786	21.653888126085405
48-49	21.55933742419106	28.468976964134836	28.3763030013798	21.59538261029431
50-51	21.516115156585233	28.54708924519473	28.125386275946706	21.811409322273335
52-53	21.544911412980703	28.576853118533062	27.86780904560377	22.010426422882464
54-55	21.6081989044882	28.542889828203926	27.91091996211565	21.93799130519222
56-57	21.766126343437705	28.53487196068627	27.7510623438254	21.94793935205062
58-59	21.663398467089248	28.51232338610315	27.951217177450626	21.873060969356974
60-61	21.761436245434336	28.45055117742386	27.894633352191285	21.89337922495052
62-63	21.827862448553294	28.386703504399357	27.738714765073198	22.046719281974152
64-65	21.708328646946352	28.44180099331672	27.739338716265415	22.110531643471518
66-67	21.865184244623077	28.382941598981127	27.596963727282365	22.154910429113432
68-69	21.907307448930144	28.326420399420797	27.59558165359048	22.170690498058583
70-71	21.901792215223935	27.941862050812034	27.728396461686227	22.427949272277807
72-73	21.99434256309728	27.973034060279346	27.838666993383974	22.193956383239403
74-75	21.97569647168533	28.092906752041692	27.636045160785798	22.295351615487185
76-77	21.98187707134868	27.887166227103766	27.513734188005483	22.617222513542067
78-79	22.113278646374958	27.792097303263695	27.52545334549569	22.569170704865655
80-81	22.00367052069955	27.785151966786646	27.662596297153456	22.548581215360343
82-83	22.345313340908486	27.562264250289974	27.772718673545622	22.319703735255914
84-85	22.619947583660238	27.570005273872244	27.48509687385671	22.324950268610806
86-87	22.096068131585092	27.78545300475102	27.627710948916317	22.49076791474757
88-89	22.37007032344804	27.48849704362066	27.53954327356815	22.60188935936315
90-91	22.605745785877986	27.304883662366958	27.584924686341907	22.504445865413153
92-93	22.5111456958562	27.3790697081367	27.634894981175297	22.474889614831806
94-95	22.28680487568232	27.408481480088078	27.774978923733286	22.529734720496315
96-97	22.49003602331084	27.463882033315766	27.765853502111792	22.280228441261603
98-99	23.57440665004324	29.40367346612735	27.913913437942362	19.108006445887046
100	22.695586665053394	29.848631911208695	21.732943380262533	25.72283804347538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2780.0
1	2791.5
2	5317.5
3	8181.5
4	12125.5
5	22104.0
6	29633.5
7	32716.5
8	32810.0
9	29651.5
10	25506.5
11	21732.0
12	23966.0
13	33923.0
14	56766.5
15	88180.0
16	112994.5
17	126273.0
18	127252.0
19	116808.0
20	99569.5
21	80261.0
22	65166.5
23	58955.0
24	60525.0
25	69126.0
26	82698.0
27	96225.0
28	107298.0
29	120926.0
30	134524.5
31	146450.5
32	162084.0
33	178826.5
34	197230.5
35	216672.5
36	236287.5
37	256833.0
38	268173.0
39	274280.5
40	285723.5
41	292603.0
42	296703.0
43	309788.0
44	326365.0
45	339676.5
46	347248.5
47	350156.0
48	349360.5
49	340324.5
50	325200.5
51	310059.0
52	293705.5
53	274234.5
54	249460.5
55	217860.5
56	187751.0
57	159202.5
58	133229.0
59	112938.5
60	91648.5
61	70007.5
62	52117.0
63	37928.0
64	26896.5
65	19133.5
66	14381.5
67	11152.5
68	8438.0
69	6331.5
70	4665.0
71	3251.0
72	2346.5
73	1718.5
74	1243.0
75	865.0
76	568.5
77	364.5
78	227.5
79	140.0
80	90.5
81	63.0
82	45.5
83	30.0
84	20.0
85	16.0
86	13.0
87	8.5
88	5.0
89	3.5
90	2.0
91	2.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.793956783291212E-5
2	5.18507427049799E-4
3	3.793956783291212E-5
4	3.793956783291212E-5
5	3.793956783291212E-5
6	3.793956783291212E-5
7	1.2646522610970706E-5
8	1.2646522610970706E-5
9	1.2646522610970706E-5
10-11	0.0016756642459536187
12-13	0.009244608028619587
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.005936903510731593
36-37	0.047544306347415737
38-39	0.1519505967079614
40-41	0.1797703349431936
42-43	0.05549351984942057
44-45	0.00482797672862016
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.014866019451535971
64-65	0.10262264692509418
66-67	0.22583973893435874
68-69	0.25112983690374835
70-71	0.11330673001026675
72-73	0.007448599761252776
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	700.0
22-23	1591.0
24-25	3065.0
26-27	5221.0
28-29	11791.0
30-31	27160.0
32-33	32425.0
34-35	41102.0
36-37	44884.0
38-39	49792.0
40-41	66303.0
42-43	80405.0
44-45	101318.0
46-47	97636.0
48-49	78805.0
50-51	64490.0
52-53	60435.0
54-55	73941.0
56-57	68795.0
58-59	66275.0
60-61	72058.0
62-63	74268.0
64-65	86745.0
66-67	103543.0
68-69	98308.0
70-71	96496.0
72-73	91466.0
74-75	92202.0
76-77	87165.0
78-79	89203.0
80-81	91209.0
82-83	92479.0
84-85	99496.0
86-87	104740.0
88-89	108821.0
90-91	105784.0
92-93	104077.0
94-95	117542.0
96-97	246313.0
98-99	440014.0
100-101	4529092.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.19530442190326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.20562705062099	65.68463996003125
2	8.021578635526534	12.224132521563593
3	2.612436815251649	5.971662552632607
4	1.2172347909204486	3.7099030178846135
5	0.6567010397742873	2.501876781989111
6	0.38467766613107807	1.7586379125098854
7	0.2578654002140352	1.375369286842903
8	0.16224166411140833	0.9889642389507949
9	0.11121578650396687	0.762670863827204
>10	0.36474066731553656	4.016426385379899
>50	0.0033303464231104053	0.16905858960394066
>100	0.0017792254954763248	0.25734334710493695
>500	2.2836468572953291E-4	0.12038119120340111
>1k	3.4254702578599054E-4	0.45893335047588374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0586090443882825E-5
2	0.0	0.0	0.0	0.0	7.587913566582423E-5
3	0.0	0.0	0.0	0.0	7.587913566582423E-5
4	0.0	0.0	0.0	0.0	1.0117218088776565E-4
5	0.0	0.0	0.0	0.0	1.0117218088776565E-4
6	0.0	0.0	0.0	0.0	1.7705131655358988E-4
7	0.0	0.0	0.0	0.0	1.896978391645606E-4
8	0.0	0.0	0.0	0.0	2.7822349744135555E-4
9	0.0	0.0	0.0	0.0	3.5410263310717977E-4
10-11	0.0	0.0	0.0	0.0	3.5410263310717977E-4
12-13	0.0	0.0	0.0	6.323261305485353E-6	3.667491557181505E-4
14-15	0.0	0.0	0.0	1.2646522610970706E-5	5.121841657443136E-4
16-17	1.2646522610970706E-5	0.0	0.0	2.5293045221941412E-5	6.196796079375646E-4
18-19	1.2646522610970706E-5	0.0	0.0	8.852565827679495E-5	7.018820049088742E-4
20-21	1.2646522610970706E-5	0.0	0.0	2.466071909139288E-4	7.33498311436301E-4
22-23	1.2646522610970706E-5	0.0	0.0	8.093774471021252E-4	8.536402762405228E-4
24-25	1.2646522610970706E-5	0.0	0.0	0.002971932813578116	0.0010180450701831418
26-27	1.2646522610970706E-5	0.0	0.0	0.0077965811896634415	0.0010370148540995979
28-29	1.2646522610970706E-5	0.0	0.0	0.029833146839279897	0.0010433381154050833
30-31	1.2646522610970706E-5	0.0	0.0	0.04046887235510626	0.0010749544219325101
32-33	1.2646522610970706E-5	0.0	0.0	0.043636826269154425	0.0012014196480422171
34-35	1.2646522610970706E-5	0.0	0.0	0.04698815476106166	0.001289945306319012
36-37	2.5293045221941412E-5	0.0	0.0	0.05166104486581534	0.0013025918289299827
38-39	2.5293045221941412E-5	0.0	0.0	0.063681564607543	0.001340531396762895
40-41	2.5293045221941412E-5	0.0	0.0	0.0764545524446234	0.0013658244419848364
42-43	3.161630652742676E-5	0.0	0.0	0.08413099166948262	0.0014037640098177484
44-45	3.793956783291212E-5	0.0	0.0	0.0932933973011309	0.001448026838956146
46-47	3.793956783291212E-5	0.0	0.0	0.10256962163627792	0.0014922896680945434
48-49	3.793956783291212E-5	0.0	0.0	0.11352151021737855	0.0015681688037603677
50-51	3.793956783291212E-5	0.0	0.0	0.12461883380850536	0.0015681688037603677
52-53	3.793956783291212E-5	0.0	0.0	0.15564707703452196	0.0016440479394261918
54-55	3.793956783291212E-5	0.0	0.0	0.19870848652487721	0.0017325735977029868
56-57	3.793956783291212E-5	0.0	0.0	0.2482765318985769	0.001751543381619443
58-59	3.793956783291212E-5	0.0	0.0	0.2786408326875176	0.0017641899042304134
60-61	3.793956783291212E-5	0.0	0.0	0.30113899641243447	0.0017705131655358988
62-63	3.793956783291212E-5	0.0	0.0	0.3237573021021556	0.0018463923012017232
64-65	3.793956783291212E-5	0.0	0.0	0.33232532117108826	0.0018463923012017232
66-67	3.793956783291212E-5	0.0	0.0	0.3418734457423711	0.0018716853464236646
68-69	3.793956783291212E-5	0.0	0.0	0.3524079990773097	0.001896978391645606
70-71	3.793956783291212E-5	0.0	0.0	0.3603183989704719	0.001947564482089489
72-73	3.793956783291212E-5	0.0	0.0	0.36587654565799355	0.0020740297081991957
74-75	3.793956783291212E-5	0.0	0.0	0.3707138405566898	0.0020740297081991957
76-77	3.793956783291212E-5	0.0	0.0	0.3751970328222789	0.0020740297081991957
78-79	3.793956783291212E-5	0.0	0.0	0.37780853974144435	0.0020740297081991957
80-81	3.793956783291212E-5	0.0	0.0	0.3786558567563794	0.002143585582559535
82-83	3.793956783291212E-5	0.0	0.0	0.3792692131030115	0.002238434502141815
84-85	3.793956783291212E-5	0.0	0.0	0.37950949703261994	0.0022510810247527856
86-87	3.793956783291212E-5	0.0	0.0	0.37971816465570096	0.0022510810247527856
88	3.793956783291212E-5	0.0	0.0	0.37973713443961743	0.0022510810247527856
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22655	0.0	45.41832	1
GTATCAA	39585	0.0	35.288754	1
TATCAAC	50265	0.0	27.561543	2
ATCAACG	50080	0.0	27.523643	3
AACGCAG	50435	0.0	27.392057	6
CAACGCA	50620	0.0	27.239193	5
TCAACGC	51105	0.0	27.052145	4
ACGCAGA	56300	0.0	24.488262	7
CGCAGAG	56650	0.0	24.322433	8
GCAGAGT	62315	0.0	21.998997	9
GTACATG	34070	0.0	20.730047	1
ACATGGG	32910	0.0	20.69785	3
TACATGG	33585	0.0	20.624996	2
GAGTACT	44200	0.0	20.404373	12-13
ACGGTAC	955	0.0	19.39386	3
CAGAGTA	61745	0.0	19.355146	10-11
GGTTCAC	3520	0.0	18.945545	6
AGAGTAC	60100	0.0	18.56976	10-11
AGTACTT	46525	0.0	18.407063	12-13
GTACTTT	49140	0.0	18.306026	14-15
>>END_MODULE