FastQCFastQC Report
Fri 27 May 2016
SRR2138758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316226
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128800.049.7727661
GTATCAA237600.036.038791
TATCAAC301050.028.2232172
TCAACGC302700.028.0830464
ATCAACG302450.028.0378423
CAACGCA306300.027.700035
AACGCAG310800.027.4102756
ACGCAGA344850.024.6819657
CGCAGAG346350.024.5392118
GCAGAGT388350.021.8426579
TACATGG237400.020.36192
GAGTACT263950.020.26939212-13
ACATGGG232200.020.194073
GTGGTAT59200.020.1338561
GTACATG246850.020.0183091
CAGAGTA385300.019.27033410-11
TGGTATC57850.018.9582712
AGAGTAC371900.018.69565410-11
AGTACTT276850.018.42783212-13
GTACTTT291400.018.28630314-15
Produced by FastQC (version 0.11.5)