##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6316226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70385464357989	33.0	33.0	33.0	33.0	33.0
2	32.63610801766751	33.0	33.0	33.0	33.0	33.0
3	32.71356487244123	33.0	33.0	33.0	33.0	33.0
4	36.6888920694098	37.0	37.0	37.0	37.0	37.0
5	36.69123888220592	37.0	37.0	37.0	37.0	37.0
6	36.683809762348595	37.0	37.0	37.0	37.0	37.0
7	36.67082653470601	37.0	37.0	37.0	37.0	37.0
8	36.66056344405663	37.0	37.0	37.0	37.0	37.0
9	36.660810585308376	37.0	37.0	37.0	37.0	37.0
10-11	36.665470409070224	37.0	37.0	37.0	37.0	37.0
12-13	36.66522144711098	37.0	37.0	37.0	37.0	37.0
14-15	38.87477490514114	40.0	37.0	40.0	37.0	40.0
16-17	38.80800932392223	40.0	37.0	40.0	37.0	40.0
18-19	38.844970794268605	40.0	37.0	40.0	37.0	40.0
20-21	38.85888195638866	40.0	37.0	40.0	37.0	40.0
22-23	38.859638034179326	40.0	37.0	40.0	37.0	40.0
24-25	38.85479930598942	40.0	37.0	40.0	37.0	40.0
26-27	38.77502515867235	40.0	37.0	40.0	37.0	40.0
28-29	38.67441963217849	40.0	37.0	40.0	37.0	40.0
30-31	38.529047476141045	40.0	37.0	40.0	37.0	40.0
32-33	38.42471388110883	40.0	37.0	40.0	37.0	40.0
34-35	38.39511103047545	40.0	37.0	40.0	37.0	40.0
36-37	38.370434925488425	40.0	37.0	40.0	37.0	40.0
38-39	38.327202138049294	40.0	37.0	40.0	37.0	40.0
40-41	38.29867685989092	40.0	37.0	40.0	37.0	40.0
42-43	38.270396741861255	40.0	37.0	40.0	37.0	40.0
44-45	38.17497765810482	40.0	37.0	40.0	37.0	40.0
46-47	37.79556769279065	40.0	37.0	40.0	33.0	40.0
48-49	37.96386091570846	40.0	37.0	40.0	33.0	40.0
50-51	37.97471412668132	40.0	37.0	40.0	33.0	40.0
52-53	37.93605957138803	40.0	37.0	40.0	33.0	40.0
54-55	37.83564313807064	40.0	37.0	40.0	33.0	40.0
56-57	37.65862306258714	40.0	37.0	40.0	33.0	40.0
58-59	37.47578656675479	40.0	37.0	40.0	33.0	40.0
60-61	37.29703694280832	37.0	37.0	40.0	33.0	40.0
62-63	37.15080576784154	37.0	37.0	40.0	33.0	40.0
64-65	36.963868955652515	37.0	37.0	40.0	33.0	40.0
66-67	36.744516032295834	37.0	37.0	40.0	33.0	40.0
68-69	36.52975857562526	37.0	37.0	40.0	33.0	40.0
70-71	36.30426175862843	37.0	37.0	38.5	33.0	40.0
72-73	36.02658025993898	37.0	37.0	37.0	33.0	40.0
74-75	35.69576963831924	37.0	35.0	37.0	33.0	40.0
76-77	33.5211301307601	35.0	33.0	37.0	27.0	37.0
78-79	35.20565534799675	37.0	33.0	37.0	33.0	37.0
80-81	35.421330925669636	37.0	33.0	37.0	33.0	37.0
82-83	35.253882211322534	37.0	33.0	37.0	33.0	37.0
84-85	35.10409379647426	37.0	33.0	37.0	33.0	37.0
86-87	35.01107634052215	37.0	33.0	37.0	33.0	37.0
88-89	34.833595879419676	37.0	33.0	37.0	33.0	37.0
90-91	34.778516955033275	37.0	33.0	37.0	33.0	37.0
92-93	34.82588555964834	37.0	33.0	37.0	33.0	37.0
94-95	34.81031686503121	37.0	33.0	37.0	33.0	37.0
96-97	34.80843192557422	37.0	33.0	37.0	33.0	37.0
98-99	34.813027871825966	37.0	33.0	37.0	33.0	37.0
100	34.76312549902892	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	6.0
20	30.0
21	129.0
22	460.0
23	1406.0
24	3595.0
25	7133.0
26	13038.0
27	21192.0
28	32704.0
29	46818.0
30	65424.0
31	89173.0
32	120423.0
33	168052.0
34	241618.0
35	376969.0
36	743002.0
37	2025306.0
38	2280131.0
39	79612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.172531852491005	20.085715755851	14.608852597872938	23.132899793785054
2	14.871010157222821	20.531775855558607	42.43518447419689	22.162029513021686
3	17.874439578317812	26.454594879917217	30.886830205252313	24.78413533651266
4	12.292577877992333	17.75200887365335	39.16585631989736	30.789556928456964
5	12.926136588526123	37.72600917066615	35.73067208171462	13.617182159093105
6	29.2925395639738	38.04111822471204	18.73154950440342	13.93479270691074
7	25.991470222883095	33.21399519269893	23.398592767263235	17.395941817154736
8	24.253438683163015	36.352562432059905	21.222609830617206	18.171389054159874
9	25.69448908256291	16.82862519485528	21.856152708911935	35.62073301366987
10-11	24.067571171598793	26.915741303747055	28.84821557139737	20.168471953256788
12-13	25.109562983881574	24.47403157184139	29.106073562072183	21.310331882204856
14-15	22.06378460808717	25.71910029818439	27.63726313782946	24.57985195589898
16-17	20.997499139517807	28.80044190945669	28.26938903072816	21.932669920297343
18-19	20.890615693611977	27.44314405469342	30.83889018537335	20.827350066321248
20-21	21.901448567708336	26.62982866457928	30.89909139155723	20.569631376155158
22-23	21.531890070864712	26.56959622916249	30.74350311792703	21.15501058204577
24-25	21.15136169901077	26.802607588772243	30.867056920740943	21.178973791476043
26-27	21.07189462959539	27.026585786535197	30.803365685702776	21.098153898166636
28-29	20.9709199309832	27.240043758940903	30.703931257782603	21.085105052293297
30-31	21.28758360681124	26.99253718009041	30.603072879131698	21.11680633396665
32-33	20.97448869838184	27.38008015106005	30.440400595954202	21.20503055460391
34-35	21.093531263520525	27.416499102479026	30.387972474796555	21.10199715920389
36-37	21.30221863138305	27.146583127323026	30.33687465757202	21.214323583721907
38-39	21.098307116640257	27.46914628094408	30.279984048251603	21.152562554164064
40-41	21.422333162202513	27.398847588006152	30.160167957360233	21.0186512924311
42-43	21.406251834521566	27.54425713514167	29.65922175440372	21.390269275933044
44-45	21.40874698263612	27.938948405797486	29.180155746356316	21.472148865210077
46-47	21.720868499528684	27.712142137741274	28.72122323934681	21.84576612338323
48-49	21.686723146075188	27.871079857875426	28.548044651352633	21.894152344696753
50-51	21.4804552050342	28.053500634182	28.427684545744192	22.038359615039603
52-53	21.78872632695212	28.04054908751289	28.12916599612503	22.041558589409963
54-55	21.81892334959087	27.909356167772124	28.122285299857168	22.149435182779836
56-57	21.86496778091109	28.056254542669862	28.07106874047523	22.00770893594382
58-59	21.833577412844065	28.06330646015205	28.107841694572457	21.995274432431426
60-61	21.83035096098529	28.01029426533178	28.055751030825903	22.103603742857022
62-63	21.941840821461252	27.97575210088149	28.03394898079688	22.048458096860383
64-65	21.891432045719274	28.023421673456074	27.956567736043166	22.128578544781487
66-67	22.007588086339336	28.08645662512241	27.702067489152345	22.203887799385914
68-69	21.956094474456897	28.038252161849147	27.848096175923104	22.157557187770852
70-71	22.071312079310314	27.580567796443766	27.81332818237488	22.534791941871035
72-73	22.11680049835171	27.62686482415051	27.8876804841533	22.368654193344476
74-75	21.977294300958704	27.755285899033566	27.820278790343412	22.44714100966432
76-77	22.04498417743981	27.76306968264705	27.588388698131304	22.603557441781835
78-79	22.19896419614196	27.767117615135824	27.543570971611032	22.490347217111186
80-81	22.104098910193493	27.711336754549183	27.657042893991697	22.527521441265627
82-83	22.414208554711525	27.320448530832998	27.845175091086126	22.42016782336935
84-85	22.620696142556973	27.45955483176166	27.518588221164293	22.40116080451707
86-87	22.1960944068185	27.589954454195677	27.65766199340195	22.55628914558388
88-89	22.384115823274787	27.352254976693523	27.691619817566536	22.57200938246515
90-91	22.517616092573352	27.291311122286693	27.71061141204518	22.480461373094776
92-93	22.545224214313674	27.301894910727363	27.720182428510665	22.4326984464483
94-95	22.295372991419335	27.390102393181742	27.751707592711096	22.562817022687828
96-97	22.491364706909543	27.334678820595382	27.812794233665723	22.361162238829355
98-99	23.659542081403394	29.351739293648933	27.926805489877214	19.06191313507046
100	22.68149221613811	30.01445749572751	21.67023246207644	25.633817826057943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1754.0
1	1753.5
2	3028.5
3	4551.5
4	6492.0
5	11442.0
6	15341.5
7	17378.5
8	18340.0
9	17250.0
10	15130.0
11	13312.5
12	14594.0
13	19748.5
14	31802.5
15	48683.0
16	62394.5
17	69883.0
18	70557.5
19	65608.0
20	57812.0
21	48804.0
22	42067.5
23	40924.0
24	45016.0
25	53950.5
26	66388.0
27	78485.5
28	87495.0
29	97663.5
30	107558.0
31	115542.5
32	127403.0
33	141827.5
34	157638.5
35	173510.0
36	192870.0
37	210586.0
38	216477.5
39	220923.5
40	231582.5
41	239256.5
42	245396.5
43	254446.5
44	262454.0
45	271265.0
46	276420.0
47	278104.0
48	280672.0
49	276174.5
50	267453.5
51	258458.5
52	246311.5
53	235660.0
54	222393.5
55	202785.5
56	179360.0
57	153270.5
58	128686.0
59	107117.0
60	84763.0
61	63928.0
62	47265.0
63	34713.0
64	24921.5
65	16998.5
66	12268.5
67	9348.0
68	6933.0
69	4878.5
70	3468.5
71	2351.0
72	1618.0
73	1150.0
74	818.0
75	564.0
76	378.5
77	259.5
78	183.0
79	126.0
80	85.5
81	58.0
82	45.5
83	36.0
84	27.0
85	18.0
86	10.5
87	7.5
88	5.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5832239061743515E-5
2	4.90799410914049E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0015911400257052233
12-13	0.008968963428477701
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.941281529071522E-6
32-33	0.0
34-35	0.005920975303051919
36-37	0.04678782734277733
38-39	0.1511584557297278
40-41	0.17910373519481662
42-43	0.05475553381274037
44-45	0.004954072733151885
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.756877340270422E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.015281506789830313
64-65	0.10450643155985974
66-67	0.23071666647635308
68-69	0.2566561872790892
70-71	0.11425695060700816
72-73	0.0075540628544937
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	460.0
22-23	1023.0
24-25	1854.0
26-27	3208.0
28-29	8779.0
30-31	19346.0
32-33	24857.0
34-35	33048.0
36-37	45513.0
38-39	52565.0
40-41	58339.0
42-43	71226.0
44-45	85964.0
46-47	80821.0
48-49	67657.0
50-51	56565.0
52-53	51711.0
54-55	55789.0
56-57	58085.0
58-59	57889.0
60-61	56035.0
62-63	53971.0
64-65	55996.0
66-67	64197.0
68-69	60571.0
70-71	60049.0
72-73	57928.0
74-75	57619.0
76-77	54530.0
78-79	59209.0
80-81	61475.0
82-83	61182.0
84-85	65504.0
86-87	68966.0
88-89	73447.0
90-91	71187.0
92-93	72074.0
94-95	79696.0
96-97	193237.0
98-99	363426.0
100-101	3791113.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.82769353061691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.66568672778658	70.85815259984604
2	7.865572366008204	12.71512145285201
3	2.2993296343491902	5.5754853303312535
4	0.9528238614895667	3.0805822026055067
5	0.4711056622939226	1.903919204621574
6	0.26099071145755137	1.2657166344017179
7	0.1454117822363223	0.8227309279236785
8	0.09203679119250434	0.5951297241639297
9	0.06461917400426904	0.4700716913356798
>10	0.17855934373321136	2.0937194130601444
>50	0.0022352713340334073	0.12314000031958534
>100	0.0011894985034778466	0.17450467888098126
>500	2.561857785421661E-4	0.14267057776454842
>1k	1.8298983270476416E-4	0.17905556189336458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.5832239061743515E-5	0.0	0.0	0.0
6	0.0	1.5832239061743515E-5	0.0	0.0	0.0
7	0.0	1.5832239061743515E-5	0.0	0.0	0.0
8	0.0	1.5832239061743515E-5	0.0	0.0	0.0
9	0.0	1.5832239061743515E-5	0.0	0.0	0.0
10-11	3.166447812348703E-5	4.749671718523055E-5	0.0	0.0	0.0
12-13	4.749671718523055E-5	4.749671718523055E-5	0.0	7.916119530871758E-6	6.332895624697406E-5
14-15	4.749671718523055E-5	5.5412836716102305E-5	0.0	1.5832239061743515E-5	8.707731483958934E-5
16-17	6.332895624697406E-5	6.332895624697406E-5	0.0	5.5412836716102305E-5	1.0290955390133285E-4
18-19	7.916119530871757E-5	6.332895624697406E-5	0.0	1.345740320248199E-4	1.4249015155569163E-4
20-21	7.916119530871757E-5	6.332895624697406E-5	0.0	2.3748358592615273E-4	1.4249015155569163E-4
22-23	1.4249015155569163E-4	6.332895624697406E-5	0.0	6.095412038771254E-4	1.4249015155569163E-4
24-25	2.058191078026657E-4	6.332895624697406E-5	0.0	0.002034442719434042	1.4249015155569163E-4
26-27	2.058191078026657E-4	6.332895624697406E-5	0.0	0.005470038595832385	1.6623851014830693E-4
28-29	2.3748358592615273E-4	6.332895624697406E-5	0.0	0.0182466555186594	1.899868687409222E-4
30-31	2.6914806404963976E-4	7.916119530871757E-5	0.0	0.02108062631071149	1.9790298827179395E-4
32-33	3.008125421731268E-4	8.707731483958934E-5	0.0	0.02610736221281506	2.2165134686440922E-4
34-35	3.0872866170399854E-4	1.0290955390133285E-4	0.0	0.032408593359388976	2.2165134686440922E-4
36-37	4.116382156053314E-4	1.1874179296307637E-4	0.0	0.042256246055793444	2.2165134686440922E-4
38-39	4.116382156053314E-4	1.2665791249394812E-4	0.0	0.0670416163069529	2.3748358592615273E-4
40-41	4.195543351362032E-4	1.2665791249394812E-4	0.0	0.0940355839072256	2.3748358592615273E-4
42-43	4.2747045466707493E-4	1.4249015155569163E-4	0.0	0.1107465122368959	2.3748358592615273E-4
44-45	4.2747045466707493E-4	1.7415462967917869E-4	0.0	0.13265041497881805	2.453997054570245E-4
46-47	4.2747045466707493E-4	1.7415462967917869E-4	0.0	0.15451473712308583	2.61231944518768E-4
48-49	4.353865741979467E-4	1.7415462967917869E-4	0.0	0.17410713296199343	2.8498030311138327E-4
50-51	4.670510523214337E-4	1.899868687409222E-4	0.0	0.1927575105767273	3.166447812348703E-4
52-53	4.90799410914049E-4	1.9790298827179395E-4	0.0	0.2353383175332865	3.403931398274856E-4
54-55	5.066316499757925E-4	2.61231944518768E-4	0.0	0.28448158758093833	3.4830925935835737E-4
56-57	5.303800085684077E-4	2.6914806404963976E-4	0.0	0.33597594512925916	3.8788985701271615E-4
58-59	5.462122476301513E-4	3.166447812348703E-4	0.0	0.3766806317570017	3.958059765435879E-4
60-61	5.699606062227665E-4	3.166447812348703E-4	0.0	0.39385069501946257	4.0372209607445966E-4
62-63	5.699606062227665E-4	3.166447812348703E-4	0.0	0.41535879178484114	4.4330269372881844E-4
64-65	5.699606062227665E-4	3.166447812348703E-4	0.0	0.4223724736891935	4.512188132596902E-4
66-67	5.699606062227665E-4	3.4830925935835737E-4	0.0	0.4280958281100138	4.828832913831772E-4
68-69	5.699606062227665E-4	3.4830925935835737E-4	0.0	0.43408041447535284	5.066316499757925E-4
70-71	5.699606062227665E-4	3.562253788892291E-4	0.0	0.4391546470946417	5.066316499757925E-4
72-73	5.778767257536383E-4	3.641414984201009E-4	0.0	0.4429068877522748	5.066316499757925E-4
74-75	5.8579284528451E-4	3.641414984201009E-4	0.0	0.44624749019430276	5.066316499757925E-4
76-77	5.8579284528451E-4	3.641414984201009E-4	0.0	0.44944560248477494	5.145477695066643E-4
78-79	5.8579284528451E-4	3.641414984201009E-4	0.0	0.4514404646065546	5.22463889037536E-4
80-81	6.332895624697406E-4	3.641414984201009E-4	0.0	0.4522874893963579	5.382961280992795E-4
82-83	6.332895624697406E-4	3.641414984201009E-4	0.0	0.4528099532853954	5.699606062227665E-4
84-85	6.491218015314841E-4	3.641414984201009E-4	0.0	0.45307910134944507	5.699606062227665E-4
86-87	6.491218015314841E-4	3.799737374818444E-4	0.0	0.4531899270228773	5.8579284528451E-4
88	6.491218015314841E-4	3.799737374818444E-4	0.0	0.4532136753814699	6.016250843462536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12880	0.0	49.772766	1
GTATCAA	23760	0.0	36.03879	1
TATCAAC	30105	0.0	28.223217	2
TCAACGC	30270	0.0	28.083046	4
ATCAACG	30245	0.0	28.037842	3
CAACGCA	30630	0.0	27.70003	5
AACGCAG	31080	0.0	27.410275	6
ACGCAGA	34485	0.0	24.681965	7
CGCAGAG	34635	0.0	24.539211	8
GCAGAGT	38835	0.0	21.842657	9
TACATGG	23740	0.0	20.3619	2
GAGTACT	26395	0.0	20.269392	12-13
ACATGGG	23220	0.0	20.19407	3
GTGGTAT	5920	0.0	20.133856	1
GTACATG	24685	0.0	20.018309	1
CAGAGTA	38530	0.0	19.270334	10-11
TGGTATC	5785	0.0	18.958271	2
AGAGTAC	37190	0.0	18.695654	10-11
AGTACTT	27685	0.0	18.427832	12-13
GTACTTT	29140	0.0	18.286303	14-15
>>END_MODULE