FastQCFastQC Report
Fri 27 May 2016
SRR2138757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8078425
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108550.051.9193881
GTATCAA200200.037.6346971
TCAACGC249900.030.0127244
ATCAACG249400.029.9697883
CAACGCA253000.029.5602785
TATCAAC257500.029.3432852
AACGCAG261100.028.7246066
ACGCAGA295250.025.3586377
CGCAGAG303300.024.643198
CTTATAC51050.022.4155351
GCAGAGT347650.021.5857329
GTACATG243450.020.227581
TACATGG242500.020.2184522
GAGTACT213250.019.70522712-13
ACATGGG244000.019.4793913
CAGAGTA342600.019.07053810-11
AGAGTAC319350.019.00287210-11
TATACAC79600.018.6293053
GTGGTAT52750.018.524481
CATGGGG176250.018.383424
Produced by FastQC (version 0.11.5)