FastQCFastQC Report
Fri 27 May 2016
SRR2138756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7785579
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116700.054.4286271
GTATCAA223200.039.366551
TCAACGC282400.031.1745764
ATCAACG284000.030.9839023
TATCAAC288850.030.5671792
CAACGCA290300.030.3556425
AACGCAG298150.029.5401846
ACGCAGA342200.025.7124677
GTGGTAT48350.025.4438741
CGCAGAG348450.025.3003068
TGGTATC50550.022.8999962
GCAGAGT399550.022.0645529
GAGTACT240300.020.43204112-13
GTACATG274450.020.2488921
TACATGG276950.019.9889892
CAGAGTA388950.019.42632310-11
ACATGGG280250.019.387813
AGAGTAC368700.019.37534910-11
CTTATAC46900.018.8531881
GTACTTT262450.018.59377514-15
Produced by FastQC (version 0.11.5)