##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7785579
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.85605283820253	28.0	18.0	31.0	18.0	33.0
2	31.51757936050742	33.0	31.0	33.0	27.0	33.0
3	31.93156462737068	33.0	31.0	33.0	29.0	34.0
4	32.62770925579202	33.0	33.0	34.0	31.0	34.0
5	32.99525712859634	34.0	33.0	34.0	32.0	34.0
6	37.08335385203849	38.0	38.0	38.0	36.0	38.0
7	37.148375477276645	38.0	38.0	38.0	36.0	38.0
8	37.240568235194836	38.0	38.0	38.0	36.0	38.0
9	37.332562677740476	38.0	38.0	38.0	37.0	38.0
10-11	37.34828154720413	38.0	38.0	38.0	37.0	38.0
12-13	37.37234045149371	38.0	38.0	38.0	37.0	38.0
14-15	37.37582259456876	38.0	38.0	38.0	37.0	38.0
16-17	37.36826060078512	38.0	38.0	38.0	37.0	38.0
18-19	37.25395978898936	38.0	38.0	38.0	37.0	38.0
20-21	37.366937478039596	38.0	38.0	38.0	37.0	38.0
22-23	37.385757441224165	38.0	38.0	38.0	37.0	38.0
24-25	37.39291852770914	38.0	38.0	38.0	37.0	38.0
26-27	37.36461817562508	38.0	38.0	38.0	37.0	38.0
28-29	37.34093268274013	38.0	38.0	38.0	37.0	38.0
30-31	37.34259476090473	38.0	38.0	38.0	37.0	38.0
32-33	37.32678267034193	38.0	38.0	38.0	37.0	38.0
34-35	37.30832115754288	38.0	38.0	38.0	37.0	38.0
36-37	37.28277618590594	38.0	38.0	38.0	37.0	38.0
38-39	37.24744145895563	38.0	38.0	38.0	37.0	38.0
40-41	37.24444347407026	38.0	38.0	38.0	37.0	38.0
42-43	37.219733807684776	38.0	38.0	38.0	37.0	38.0
44-45	37.1766681614165	38.0	38.0	38.0	37.0	38.0
46-47	37.1280234177091	38.0	38.0	38.0	36.0	38.0
48-49	37.08664834424779	38.0	38.0	38.0	36.0	38.0
50-51	37.05531433997638	38.0	38.0	38.0	36.0	38.0
52-53	37.03537787697783	38.0	38.0	38.0	36.0	38.0
54-55	37.02264251047232	38.0	38.0	38.0	36.0	38.0
56-57	37.012704156665606	38.0	38.0	38.0	36.0	38.0
58-59	37.00411001296186	38.0	38.0	38.0	36.0	38.0
60-61	37.00587954326218	38.0	38.0	38.0	36.0	38.0
62-63	37.005921433931285	38.0	38.0	38.0	36.0	38.0
64-65	37.01355327800888	38.0	38.0	38.0	36.0	38.0
66-67	37.00985551284857	38.0	38.0	38.0	36.0	38.0
68-69	37.014422622558214	38.0	38.0	38.0	36.0	38.0
70-71	37.009828415330226	38.0	38.0	38.0	36.0	38.0
72-73	36.98561598877181	38.0	38.0	38.0	36.0	38.0
74-75	37.00803756509869	38.0	38.0	38.0	36.0	38.0
76-77	36.99663402438074	38.0	38.0	38.0	36.0	38.0
78-79	36.9898096766121	38.0	38.0	38.0	36.0	38.0
80-81	36.969553258855896	38.0	38.0	38.0	36.0	38.0
82-83	36.96040300162985	38.0	38.0	38.0	36.0	38.0
84-85	36.94641976552704	38.0	38.0	38.0	36.0	38.0
86-87	36.91276092024532	38.0	38.0	38.0	35.0	38.0
88-89	36.89785611940006	38.0	38.0	38.0	35.0	38.0
90-91	36.90649995196976	38.0	38.0	38.0	35.0	38.0
92-93	36.87679870823701	38.0	38.0	38.0	35.0	38.0
94-95	36.821667556673646	38.0	38.0	38.0	35.0	38.0
96-97	36.83887619520179	38.0	38.0	38.0	35.0	38.0
98-99	36.868291204862444	38.0	38.0	38.0	35.0	38.0
100	35.313205768790226	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	16.0
19	25.0
20	94.0
21	211.0
22	633.0
23	1673.0
24	3947.0
25	7730.0
26	13495.0
27	22236.0
28	34249.0
29	51577.0
30	70307.0
31	92240.0
32	119001.0
33	156732.0
34	225378.0
35	384933.0
36	1097847.0
37	5503255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.082265172570985	18.959848715169418	15.638091913266825	23.31979419899278
2	14.902488305622485	20.594178030946704	41.51285344352681	22.990480219904004
3	18.47483661780325	25.653827416047026	30.431468231200277	25.439867734949452
4	12.760348331190269	17.50531334920627	38.1308313742626	31.603506945340865
5	13.655213410917764	37.22360996239165	34.99922096161723	14.121955665073353
6	30.451713055525087	37.12677389249485	18.389989059235546	14.031523992744518
7	26.83014326872799	32.634708349886374	22.503361663917353	18.031786717468286
8	24.855441066104397	36.16917893967809	20.73242080004583	18.242959194171686
9	26.001393088426695	16.58147711300598	20.865513020932674	36.55161677763465
10-11	24.6394327255558	26.470035176574534	28.2818336311275	20.608698466742165
12-13	25.541550958608923	24.089712793981846	28.50755406293829	21.86118218447094
14-15	22.664079832228246	25.17640470472486	26.52735779715763	25.632157665889267
16-17	21.977633262728435	28.28206482780536	27.148057967172385	22.592243942293823
18-19	21.99785654991106	27.063908798562057	29.287417159340368	21.650817492186516
20-21	22.956886353681924	26.236026855154538	29.24906909923229	21.55801769193125
22-23	22.64991908242982	26.17582882546018	29.09834101961405	22.075911072495952
24-25	22.323490389487066	26.317309046101485	29.250813679475545	22.108386884935907
26-27	22.193994754988697	26.475943148723257	29.228835331004614	22.10122676528343
28-29	22.110712034193373	26.492094614003936	29.291211118115847	22.105982233686845
30-31	22.423337413290195	26.321749382232944	29.117788185202294	22.137125019274563
32-33	22.277957924758347	26.457463125444264	29.118014305592478	22.146564644204915
34-35	22.386879017451427	26.5512281178609	29.09144229491049	21.970450569777178
36-37	22.315347163964624	26.440365684583035	29.038273263388753	22.206013888063588
38-39	22.147651225612716	26.591184298307063	29.15483157239046	22.106332903689758
40-41	22.44487752022306	26.400340159429387	29.0026543121266	22.152128008220956
42-43	22.352896803740556	26.506602028887517	28.850745359675127	22.289755807696796
44-45	22.38103456756613	26.673753606008994	28.67232508031713	22.272886746107748
46-47	22.519912736830836	26.646645014303644	28.316276466978042	22.51716578188748
48-49	22.435927711818174	26.735422075099375	28.29294227525885	22.535707937823606
50-51	22.423873550744712	26.911947003661346	28.19057194993208	22.47360749566186
52-53	22.556122697656562	26.928148517553396	28.077003675928957	22.438725108861085
54-55	22.545011614882448	26.809293598575483	28.035177551585416	22.61051723495665
56-57	22.599532303890843	26.930925086789003	27.936036674850484	22.533505934469666
58-59	22.485406738170997	27.024492583788035	28.058754944864333	22.431345733176634
60-61	22.622645433141837	27.03582707323506	27.954855436070876	22.386672057552236
62-63	22.521931698409578	27.15994325598794	27.925017435661676	22.393107609940806
64-65	22.52524854217412	27.048761331862636	27.921101765091695	22.504888360871554
66-67	22.586636623052165	27.066183065533767	27.787619743818748	22.559560567595323
68-69	22.555777806865045	26.980696091101326	27.946912867859204	22.516613234174425
70-71	22.56223919956479	26.763405189858357	27.936680781123957	22.737674829452896
72-73	22.674366180985	26.76433738871729	27.86033123376229	22.700965196535414
74-75	22.50915224042546	26.91487552322527	27.845493515284524	22.73047872106475
76-77	22.68118481729478	26.806740540718792	27.757680429409227	22.7543942125772
78-79	22.604739258371968	26.871893655303058	27.66902031841069	22.85434676791428
80-81	22.638370930140738	26.952632851283116	27.63252063645999	22.776475582116156
82-83	22.719028417285895	26.92449546752698	27.708761105066866	22.64771501012026
84-85	22.850600542283132	26.918522370676058	27.544312282146898	22.686564804893912
86-87	22.61589265155937	26.975834668954153	27.639409789966233	22.768862889520243
88-89	22.71019526451777	26.94092376766357	27.570377333285546	22.778503634533116
90-91	22.76492288215334	27.019709998297287	27.516659108192233	22.698708011357144
92-93	22.852168678840336	26.9751874778551	27.48800440529991	22.684639438004652
94-95	22.715910470158093	27.053883115070715	27.502293226241477	22.727913188529712
96-97	22.804050688419153	27.132725181076182	27.522793310239656	22.540430820265005
98-99	23.898811491917304	29.05506185319583	27.70919587377148	19.336930781115388
100	22.7807586224031	29.90932785154409	21.6218907438661	25.688022782186714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2856.0
1	2598.5
2	3107.0
3	4160.0
4	5007.0
5	6549.0
6	8183.5
7	11270.0
8	15010.5
9	16749.5
10	16044.5
11	15033.0
12	16294.0
13	19839.5
14	26411.5
15	33465.5
16	37926.5
17	39539.0
18	37909.0
19	33827.0
20	29350.0
21	25779.5
22	24816.5
23	27462.5
24	33519.5
25	42949.0
26	55254.5
27	68418.5
28	81373.0
29	96471.5
30	111016.5
31	125103.0
32	141806.0
33	158600.5
34	175500.0
35	191737.0
36	209549.5
37	226934.5
38	239315.0
39	249522.0
40	262159.5
41	273613.5
42	281653.5
43	291638.0
44	303677.5
45	316945.0
46	323264.5
47	325538.0
48	328571.0
49	328084.5
50	325534.0
51	321023.5
52	312852.5
53	301058.5
54	287395.5
55	270046.0
56	250619.5
57	229934.5
58	206198.0
59	180777.0
60	153299.5
61	125370.0
62	100156.5
63	77739.0
64	58807.0
65	44303.5
66	33341.5
67	25189.5
68	18858.5
69	14045.0
70	10419.0
71	7462.0
72	5340.5
73	3626.5
74	2364.0
75	1538.0
76	980.5
77	594.5
78	336.0
79	184.5
80	124.5
81	95.5
82	73.5
83	53.0
84	31.5
85	18.0
86	10.5
87	6.0
88	3.0
89	1.5
90	1.5
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.00436704835953755
6	2.1835241797687753E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.4221299404963965E-6
14-15	1.9266389821489194E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.8673503970286375E-5
34-35	1.9387062509124036E-4
36-37	2.1407323964493682E-4
38-39	1.8265091580843025E-4
40-41	1.5096586648631277E-4
42-43	2.2458301539424094E-4
44-45	1.996816940675434E-4
46-47	2.68650778032802E-5
48-49	3.5236356330363883E-4
50-51	4.987717915445473E-4
52-53	4.1305495999149655E-4
54-55	0.002157344947942087
56-57	0.003189307650267797
58-59	0.0019821035098186073
60-61	0.0034361801742221606
62-63	0.0019514072336513865
64-65	0.002640820868417663
66-67	8.032206519420743E-4
68-69	7.152958721823795E-4
70-71	4.0225408289756425E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	59.0
20-21	284.0
22-23	704.0
24-25	773.0
26-27	2153.0
28-29	5647.0
30-31	14454.0
32-33	18698.0
34-35	25848.0
36-37	40051.0
38-39	47788.0
40-41	46671.0
42-43	54830.0
44-45	65811.0
46-47	67217.0
48-49	62366.0
50-51	55922.0
52-53	54016.0
54-55	58403.0
56-57	60341.0
58-59	60355.0
60-61	59404.0
62-63	58042.0
64-65	61870.0
66-67	66471.0
68-69	67364.0
70-71	71440.0
72-73	61323.0
74-75	61819.0
76-77	64022.0
78-79	65780.0
80-81	65797.0
82-83	61837.0
84-85	60651.0
86-87	62997.0
88-89	65110.0
90-91	65746.0
92-93	65477.0
94-95	76535.0
96-97	241283.0
98-99	528507.0
100-101	5111713.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.99087288724883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.95136140248033	76.45044722993187
2	6.960916000058064	11.832286538795028
3	1.6965466591710407	4.325729443706778
4	0.6281161323019452	2.1353655343562
5	0.2877305007693665	1.222723320833684
6	0.14604758376282947	0.744762697624588
7	0.09545715608821623	0.5679090913490299
8	0.06322527141906399	0.4298856805151579
9	0.04072542247368376	0.3115160284266327
>10	0.12654927230413957	1.652321870083605
>50	0.0025688663243814665	0.14554599862562712
>100	6.315654831531753E-4	0.08051375994141158
>500	7.450072712269708E-5	0.04160036978998247
>1k	4.9666636677361204E-5	0.05939243602047961
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.568851976198559E-5
2	0.0	0.0	0.0	0.0	2.568851976198559E-5
3	0.0	0.0	0.0	0.0	2.568851976198559E-5
4	0.0	2.568851976198559E-5	0.0	0.0	2.568851976198559E-5
5	0.0	3.853277964297838E-5	0.0	0.0	2.568851976198559E-5
6	7.706555928595676E-5	3.853277964297838E-5	0.0	0.0	2.568851976198559E-5
7	7.706555928595676E-5	3.853277964297838E-5	0.0	0.0	2.568851976198559E-5
8	8.990981916694956E-5	3.853277964297838E-5	0.0	0.0	2.568851976198559E-5
9	8.990981916694956E-5	1.2844259880992795E-4	0.0	1.2844259880992795E-5	8.990981916694956E-5
10-11	9.633194910744597E-5	1.2844259880992795E-4	0.0	1.926638982148919E-5	9.633194910744597E-5
12-13	1.0275407904794236E-4	1.2844259880992795E-4	0.0	2.568851976198559E-5	1.0275407904794236E-4
14-15	1.0275407904794236E-4	1.7981963833389912E-4	0.0	2.568851976198559E-5	1.0275407904794236E-4
16-17	1.5413111857191352E-4	1.926638982148919E-4	0.0	3.853277964297838E-5	1.0275407904794236E-4
18-19	1.5413111857191352E-4	2.119302880363811E-4	0.0	3.853277964297838E-5	1.0275407904794236E-4
20-21	1.5413111857191352E-4	2.504630676793595E-4	0.0	3.853277964297838E-5	1.0275407904794236E-4
22-23	1.7981963833389912E-4	3.0826223714382705E-4	0.0	3.853277964297838E-5	1.0917620898843875E-4
24-25	2.183524179768775E-4	3.4679501678680546E-4	0.0	4.495490958347478E-5	1.1559833892893516E-4
26-27	2.440409377388631E-4	3.72483536548791E-4	0.0	8.348768922645317E-5	1.2844259880992795E-4
28-29	2.440409377388631E-4	3.72483536548791E-4	0.0	3.72483536548791E-4	1.2844259880992795E-4
30-31	2.440409377388631E-4	3.9817205631077665E-4	0.0	0.0012458932084563011	1.2844259880992795E-4
32-33	2.568851976198559E-4	3.9817205631077665E-4	0.0	0.002652339665425012	1.7981963833389912E-4
34-35	2.8899584732233784E-4	4.752376155967334E-4	0.0	0.0050670605230516575	1.990860281553883E-4
36-37	3.0826223714382705E-4	5.523031748826901E-4	0.0	0.008156105024430425	2.3119667785787029E-4
38-39	3.0826223714382705E-4	5.908359545256685E-4	0.0	0.013088300818731658	2.440409377388631E-4
40-41	3.211064970248199E-4	6.293687341686469E-4	0.0	0.0180012302232114	2.825737173818415E-4
42-43	3.211064970248199E-4	6.486351239901361E-4	0.0	0.023004069446858094	3.0826223714382705E-4
44-45	3.211064970248199E-4	7.064342934546036E-4	0.0	0.02845003563639904	3.0826223714382705E-4
46-47	3.211064970248199E-4	7.44967073097582E-4	0.0	0.03448683778046566	3.0826223714382705E-4
48-49	3.2752862696531625E-4	7.44967073097582E-4	0.0	0.04078052512215212	3.0826223714382705E-4
50-51	3.5963927666779824E-4	8.09188372502546E-4	0.0	0.047331097661458446	3.0826223714382705E-4
52-53	3.789056664892874E-4	8.09188372502546E-4	0.0	0.05343212110493002	3.0826223714382705E-4
54-55	3.9174992637028023E-4	8.09188372502546E-4	0.0	0.05940470194959167	3.0826223714382705E-4
56-57	4.1101631619176943E-4	8.220326323835389E-4	0.0	0.06520388528585992	3.1468436708432347E-4
58-59	4.238605760727622E-4	8.284547623240352E-4	0.0	0.07071407277480583	3.211064970248199E-4
60-61	4.4312696589425137E-4	8.477211521455244E-4	0.0	0.07698207159673032	3.211064970248199E-4
62-63	4.752376155967334E-4	8.605654120265173E-4	0.0	0.08330786958811927	3.3395075690581267E-4
64-65	4.880818754777262E-4	9.37630971312474E-4	0.0	0.08898503245551809	3.4679501678680546E-4
66-67	5.00926135358719E-4	9.504752311934668E-4	0.0	0.09462366254327392	3.4679501678680546E-4
68-69	5.00926135358719E-4	9.633194910744595E-4	0.0	0.10057697699811408	3.4679501678680546E-4
70-71	5.073482652992155E-4	9.825858808959489E-4	0.0	0.10512384499598552	3.4679501678680546E-4
72-73	5.330367850612009E-4	9.890080108364452E-4	0.0	0.10922758602796273	3.5963927666779824E-4
74-75	5.394589150016974E-4	0.001014696530598431	0.0	0.11242580673832993	3.5963927666779824E-4
76-77	5.394589150016974E-4	0.001053229310241409	0.0	0.11535429799119629	3.853277964297838E-4
78-79	5.394589150016974E-4	0.0010789178300033948	0.0	0.11715891650447577	4.1101631619176943E-4
80-81	5.523031748826901E-4	0.0010789178300033948	0.0	0.1178203958883469	4.1101631619176943E-4
82-83	5.779916946446758E-4	0.0010789178300033948	0.0	0.11834058841352711	4.3028270601325864E-4
84-85	5.779916946446758E-4	0.0010917620898843875	0.0	0.118533252311742	4.495490958347478E-4
86-87	5.779916946446758E-4	0.0011110284797058766	0.0	0.11867453917043291	4.559712257752442E-4
88	5.779916946446758E-4	0.0012202046886943155	0.0	0.11869380556025441	4.623933557157406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11670	0.0	54.428627	1
GTATCAA	22320	0.0	39.36655	1
TCAACGC	28240	0.0	31.174576	4
ATCAACG	28400	0.0	30.983902	3
TATCAAC	28885	0.0	30.567179	2
CAACGCA	29030	0.0	30.355642	5
AACGCAG	29815	0.0	29.540184	6
ACGCAGA	34220	0.0	25.712467	7
GTGGTAT	4835	0.0	25.443874	1
CGCAGAG	34845	0.0	25.300306	8
TGGTATC	5055	0.0	22.899996	2
GCAGAGT	39955	0.0	22.064552	9
GAGTACT	24030	0.0	20.432041	12-13
GTACATG	27445	0.0	20.248892	1
TACATGG	27695	0.0	19.988989	2
CAGAGTA	38895	0.0	19.426323	10-11
ACATGGG	28025	0.0	19.38781	3
AGAGTAC	36870	0.0	19.375349	10-11
CTTATAC	4690	0.0	18.853188	1
GTACTTT	26245	0.0	18.593775	14-15
>>END_MODULE