FastQCFastQC Report
Fri 27 May 2016
SRR2138755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7458034
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100250.051.9751661
CTTATAC60800.038.0338481
GTATCAA181550.036.8463671
TATACAC82950.030.7634143
TCAACGC224500.029.9684964
ATCAACG229050.029.2798753
TATCAAC231750.029.030972
CAACGCA233350.028.7769625
TTATACA85400.028.229152
AACGCAG240100.028.0554566
GTGGTAT46400.024.412061
TACACAT102900.024.3836825
ACGCAGA275750.024.3661777
CGCAGAG282900.023.7503478
TGGTATC47950.022.9989342
GTACATG263750.022.7212431
TACATGG273150.022.0332222
ACACATC107750.021.8965196
ACATGGG275200.021.3410053
ACATCTC117700.020.5900298
Produced by FastQC (version 0.11.5)