##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7458034
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.705493699814188	25.0	18.0	31.0	18.0	33.0
2	31.47147599488015	32.0	31.0	33.0	27.0	33.0
3	31.870290213211685	33.0	31.0	33.0	29.0	34.0
4	32.60111941028963	33.0	33.0	34.0	31.0	34.0
5	32.977721474587	34.0	33.0	34.0	31.0	34.0
6	37.06126346433926	38.0	38.0	38.0	36.0	38.0
7	37.131634556774614	38.0	38.0	38.0	36.0	38.0
8	37.22375910327038	38.0	38.0	38.0	36.0	38.0
9	37.319990362071294	38.0	38.0	38.0	37.0	38.0
10-11	37.34064982809143	38.0	38.0	38.0	37.0	38.0
12-13	37.36503071721046	38.0	38.0	38.0	37.0	38.0
14-15	37.36778553168301	38.0	38.0	38.0	37.0	38.0
16-17	37.35906118153926	38.0	38.0	38.0	37.0	38.0
18-19	37.245986609876006	38.0	38.0	38.0	37.0	38.0
20-21	37.36117280270867	38.0	38.0	38.0	37.0	38.0
22-23	37.383017890943705	38.0	38.0	38.0	37.0	38.0
24-25	37.39202184640325	38.0	38.0	38.0	37.0	38.0
26-27	37.36485446422653	38.0	38.0	38.0	37.0	38.0
28-29	37.34572152394053	38.0	38.0	38.0	37.0	38.0
30-31	37.34795326621388	38.0	38.0	38.0	37.0	38.0
32-33	37.33278173505676	38.0	38.0	38.0	37.0	38.0
34-35	37.314960500608464	38.0	38.0	38.0	37.0	38.0
36-37	37.29604173924718	38.0	38.0	38.0	37.0	38.0
38-39	37.256613627218485	38.0	38.0	38.0	37.0	38.0
40-41	37.27814026843513	38.0	38.0	38.0	37.0	38.0
42-43	37.265775026852104	38.0	38.0	38.0	37.0	38.0
44-45	37.238647146147166	38.0	38.0	38.0	37.0	38.0
46-47	37.20821540607312	38.0	38.0	38.0	37.0	38.0
48-49	37.18326090689568	38.0	38.0	38.0	37.0	38.0
50-51	37.1592150488402	38.0	38.0	38.0	36.5	38.0
52-53	37.14039871228775	38.0	38.0	38.0	36.0	38.0
54-55	37.126230232785154	38.0	38.0	38.0	36.0	38.0
56-57	37.11562619926083	38.0	38.0	38.0	36.0	38.0
58-59	37.10454323493627	38.0	38.0	38.0	36.0	38.0
60-61	37.10065343280891	38.0	38.0	38.0	36.0	38.0
62-63	37.09469122997738	38.0	38.0	38.0	36.0	38.0
64-65	37.10074776768734	38.0	38.0	38.0	36.0	38.0
66-67	37.08954876856282	38.0	38.0	38.0	36.0	38.0
68-69	37.08911160653865	38.0	38.0	38.0	36.0	38.0
70-71	37.081204929203395	38.0	38.0	38.0	36.0	38.0
72-73	37.049185111288665	38.0	38.0	38.0	36.0	38.0
74-75	37.06913968681	38.0	38.0	38.0	36.0	38.0
76-77	37.05998328178623	38.0	38.0	38.0	36.0	38.0
78-79	37.05141662197772	38.0	38.0	38.0	36.0	38.0
80-81	37.02940270758316	38.0	38.0	38.0	36.0	38.0
82-83	37.02136922953946	38.0	38.0	38.0	36.0	38.0
84-85	37.00445904520842	38.0	38.0	38.0	36.0	38.0
86-87	36.97038144463494	38.0	38.0	38.0	36.0	38.0
88-89	36.9553099474922	38.0	38.0	38.0	36.0	38.0
90-91	36.95904523564242	38.0	38.0	38.0	36.0	38.0
92-93	36.927188099244376	38.0	38.0	38.0	35.0	38.0
94-95	36.867812079186336	38.0	38.0	38.0	35.0	38.0
96-97	36.88188562356984	38.0	38.0	38.0	35.0	38.0
98-99	36.91328402121664	38.0	38.0	38.0	35.0	38.0
100	35.41609971462419	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	14.0
19	13.0
20	74.0
21	198.0
22	565.0
23	1491.0
24	3421.0
25	6701.0
26	11749.0
27	18848.0
28	29032.0
29	42268.0
30	59559.0
31	79881.0
32	105253.0
33	142323.0
34	210552.0
35	370353.0
36	1068007.0
37	5307727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1451417357443	18.627254850272873	15.334778575694344	23.892824838288483
2	15.253470284528067	20.21444257293544	40.76870660552097	23.763380537015518
3	18.77462612801175	25.129303513499668	29.992300920054802	26.103769438433776
4	13.301146119741475	17.23367042842658	36.724718605466265	32.74046484636568
5	14.26528299624994	36.832347133163104	34.451427635872435	14.450942234714523
6	31.49778634067814	36.11569194100359	17.91631363474427	14.470208083574002
7	27.731892882226067	31.787934997346483	21.84815730258135	18.632014817846095
8	25.47810857392176	35.794017565487096	20.3267509909448	18.401122869646343
9	26.188858886939908	16.458519765396616	20.222474180192794	37.13014716747068
10-11	25.3071654004259	25.908992906173395	27.684836245047954	21.09900544835274
12-13	26.092501461678875	23.352100791716744	27.909830386253965	22.64556736035042
14-15	23.237477866150787	24.527536345369302	25.368012535207	26.866973253272914
16-17	22.97199905497883	27.787805740762245	25.985809396953673	23.25438580730525
18-19	23.137069367074485	26.652023844353618	27.68868444418462	22.522222344387274
20-21	23.750082058622343	25.812682108953865	27.650225281512203	22.78701055091158
22-23	23.656001964930393	25.88294766924083	27.49536300304894	22.965687362779835
24-25	23.500788769023902	26.104001590686433	27.51194557748653	22.883264062803136
26-27	23.343455072662145	26.197356135931237	27.564854449254256	22.894334342152366
28-29	23.192317524010424	26.14885924983943	27.573957847772522	23.08486537837762
30-31	23.69760440815778	25.968121493129054	27.42594496522528	22.908329133487886
32-33	23.08085129531694	26.388218526753	27.47807980336473	23.052850374565335
34-35	23.396826922440905	26.20350445410412	27.558027005541526	22.841641617913446
36-37	23.227291071298037	26.21079204783639	27.496161627411713	23.065755253453858
38-39	23.10412569124107	26.049445413308824	27.743971963749914	23.102456931700193
40-41	23.325085443656512	25.85630174327873	27.674728826391547	23.14388398667321
42-43	23.3822199208698	25.954502685291448	27.455452911991053	23.207824481847698
44-45	23.21335349701649	26.11999380739084	27.411698243953964	23.2549544516387
46-47	23.59409521143113	25.99078970960938	27.02818039363998	23.38693468531951
48-49	23.354744485397173	26.15627096878382	26.942318941658872	23.546665604160136
50-51	23.247154376544643	26.360298353431382	26.969555570436587	23.42299169958739
52-53	23.547409655305476	26.249736392505778	26.89760429776758	23.30524965442116
54-55	23.395795580465922	26.191525672431727	26.709499229823436	23.703179517278915
56-57	23.553555151771366	26.453728696746143	26.538957008249543	23.453759143232947
58-59	23.307343758776025	26.661676531300373	26.784009491780775	23.246970218142827
60-61	23.54931170588566	26.811506015355963	26.490418069460116	23.14876420929826
62-63	23.32936143307236	27.122667982573194	26.403315200810084	23.144655383544364
64-65	23.38391668758567	27.04861756991613	26.32120061233986	23.24626513015834
66-67	23.383344050005906	26.96353811989431	26.31491673468241	23.33820109541738
68-69	23.290773373472128	26.865112977110723	26.46247374066904	23.381639908748106
70-71	23.43652413851768	26.573243450301987	26.52181711456966	23.46841529661067
72-73	23.569949104729634	26.441833662517666	26.382966957350153	23.605250275402547
74-75	23.35342777493676	26.56074192129895	26.523902023626363	23.561928280137938
76-77	23.679673131081884	26.378112464124943	26.37150611095666	23.57070829383651
78-79	23.467523618096784	26.416215780193543	26.386154439070154	23.730106162639526
80-81	23.571523392002653	26.487928176047742	26.354698280234356	23.58585015171525
82-83	23.59911715322091	26.445868250398767	26.37959217076143	23.575422425618893
84-85	23.596358288598076	26.47570260304234	26.325132194735513	23.602806913624075
86-87	23.517640595930814	26.456930452509514	26.453684203374237	23.571744748185434
88-89	23.547285560310776	26.429803361189492	26.372832755565266	23.65007832293447
90-91	23.591320847052504	26.448079412841953	26.355340687249434	23.60525905285611
92-93	23.64839218228991	26.4850803469758	26.28173730851583	23.58479016221846
94-95	23.592891890910792	26.444629009310905	26.352084161579103	23.610394938199196
96-97	23.715363201087467	26.491565375556224	26.397147606327465	23.395923817028844
98-99	24.837586104766803	28.48588942618035	26.478514146814796	20.19801032223805
100	23.686925228280295	29.11645450067375	20.47003553966968	26.726584731376278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2160.0
1	1864.0
2	1717.0
3	2284.5
4	3245.0
5	4323.5
6	5067.5
7	5128.0
8	4904.5
9	5056.0
10	5448.0
11	6635.5
12	8589.0
13	12947.0
14	19368.0
15	25546.0
16	30308.5
17	31643.0
18	30811.5
19	27128.5
20	22987.5
21	20826.5
22	20277.5
23	22234.5
24	26415.0
25	33480.0
26	42377.5
27	51825.0
28	62136.0
29	74176.5
30	86629.5
31	99810.5
32	114631.5
33	128729.0
34	141413.5
35	153650.0
36	169178.5
37	186643.0
38	201960.0
39	213819.0
40	225578.0
41	238069.0
42	251465.0
43	276353.0
44	295710.0
45	302739.5
46	306216.5
47	306615.5
48	312814.5
49	318090.5
50	320319.0
51	323435.5
52	322346.5
53	315971.0
54	310232.0
55	299512.0
56	281730.5
57	261266.5
58	237642.5
59	211746.5
60	182164.5
61	152292.5
62	124582.0
63	98814.0
64	75973.5
65	56839.5
66	41980.5
67	31809.5
68	24512.0
69	18207.0
70	13358.5
71	9604.5
72	6516.5
73	4295.0
74	2830.5
75	1855.5
76	1191.5
77	706.0
78	411.5
79	250.5
80	169.0
81	105.5
82	68.0
83	49.5
84	35.0
85	20.5
86	11.0
87	9.0
88	4.5
89	2.0
90	2.5
91	3.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0038213824179401702
6	2.815755465850652E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.704179680596791E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	5.386084283735957E-5
34-35	1.2824153253221073E-4
36-37	1.3549370824803722E-4
38-39	1.702812958461104E-4
40-41	8.22116411272778E-5
42-43	1.7227954575258907E-4
44-45	1.5266382059669488E-4
46-47	6.296243283482478E-5
48-49	4.090163277202424E-4
50-51	3.558150823324077E-4
52-53	4.234928875087327E-4
54-55	0.0020527982603835812
56-57	0.0034524250008011958
58-59	0.002077752280241354
60-61	0.0034717007165990005
62-63	0.0021194959898240312
64-65	0.0026787646500585263
66-67	7.368053729366981E-4
68-69	7.062314950076341E-4
70-71	3.958244402188676E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.749429707783134E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	703.0
20-21	2283.0
22-23	1352.0
24-25	1437.0
26-27	2626.0
28-29	5604.0
30-31	13627.0
32-33	17212.0
34-35	24066.0
36-37	37463.0
38-39	43194.0
40-41	41668.0
42-43	47725.0
44-45	57204.0
46-47	57857.0
48-49	53978.0
50-51	72609.0
52-53	47247.0
54-55	51922.0
56-57	54238.0
58-59	55341.0
60-61	55821.0
62-63	53915.0
64-65	59548.0
66-67	67499.0
68-69	69996.0
70-71	81584.0
72-73	58110.0
74-75	56237.0
76-77	58555.0
78-79	60468.0
80-81	59570.0
82-83	56631.0
84-85	55779.0
86-87	57797.0
88-89	61013.0
90-91	62045.0
92-93	61863.0
94-95	70792.0
96-97	235881.0
98-99	512754.0
100-101	4912820.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.50973613136887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90679459558703	75.9799948769024
2	6.937548791787684	11.725808355849482
3	1.6771605860117407	4.252091957213525
4	0.618721285753543	2.0915189039157274
5	0.3150129464621445	1.331083049174043
6	0.16811339811473996	0.8524331348894663
7	0.10791422869244006	0.6383862094132813
8	0.06724133581899355	0.45460380377471254
9	0.04660405082464331	0.3544646434059159
>10	0.15045068649160728	2.020625080797495
>50	0.0035268086561687	0.19928734907626236
>100	9.112857992850264E-4	0.09970263558769166
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.340835936119358E-5	0.0	0.0	0.0
3	0.0	1.340835936119358E-5	0.0	0.0	0.0
4	0.0	9.385851552835505E-5	0.0	0.0	0.0
5	1.340835936119358E-5	1.0726687488954864E-4	0.0	0.0	0.0
6	4.022507808358074E-5	1.0726687488954864E-4	0.0	0.0	0.0
7	4.022507808358074E-5	1.0726687488954864E-4	0.0	0.0	0.0
8	4.022507808358074E-5	1.0726687488954864E-4	0.0	0.0	0.0
9	4.022507808358074E-5	1.7430867169551653E-4	0.0	0.0	1.340835936119358E-5
10-11	4.022507808358074E-5	1.7430867169551653E-4	0.0	0.0	1.340835936119358E-5
12-13	4.022507808358074E-5	1.7430867169551653E-4	0.0	0.0	2.011253904179037E-5
14-15	5.363343744477432E-5	2.3464628882088765E-4	0.0	0.0	3.352089840298395E-5
16-17	1.0726687488954864E-4	2.4135046850148445E-4	0.0	0.0	4.022507808358074E-5
18-19	1.0726687488954864E-4	2.5475882786267804E-4	0.0	0.0	4.6929257764177527E-5
20-21	1.0726687488954864E-4	2.815755465850652E-4	0.0	0.0	6.033761712537111E-5
22-23	1.0726687488954864E-4	3.2180062466864593E-4	0.0	0.0	6.70417968059679E-5
24-25	1.2737941393133902E-4	3.82138241794017E-4	0.0	0.0	7.374597648656468E-5
26-27	1.474919529731294E-4	4.1565914019700095E-4	0.0	2.681671872238716E-5	8.045015616716148E-5
28-29	1.474919529731294E-4	4.1565914019700095E-4	0.0	2.9498390594625873E-4	8.045015616716148E-5
30-31	1.6760449201491976E-4	4.894051166835656E-4	0.0	8.983600771999699E-4	8.715433584775827E-5
32-33	1.7430867169551653E-4	5.162218354059528E-4	0.0	0.0024068005053342477	9.385851552835505E-5
34-35	1.810128513761133E-4	5.899678118925175E-4	0.0	0.004672813237375962	1.0726687488954864E-4
36-37	1.877170310567101E-4	6.570096086984855E-4	0.0	0.007723214992047502	1.0726687488954864E-4
38-39	1.877170310567101E-4	6.70417968059679E-4	0.0	0.012637378697924948	1.2067523425074222E-4
40-41	1.877170310567101E-4	8.045015616716148E-4	0.0	0.017383937911787474	1.2067523425074222E-4
42-43	1.877170310567101E-4	8.179099210328083E-4	0.0	0.022143905485011196	1.2737941393133902E-4
44-45	1.877170310567101E-4	9.58697694325341E-4	0.0	0.027594403565336385	1.474919529731294E-4
46-47	1.877170310567101E-4	0.00108607710825668	0.0	0.03350749004362276	1.474919529731294E-4
48-49	1.877170310567101E-4	0.00108607710825668	0.0	0.039507730857756884	1.7430867169551653E-4
50-51	1.877170310567101E-4	0.0011665272644238414	0.0	0.04559512600773877	1.7430867169551653E-4
52-53	1.877170310567101E-4	0.0011799356237850351	0.0	0.05168922533740125	1.877170310567101E-4
54-55	1.877170310567101E-4	0.0012000481628268254	0.0	0.057173244316129426	2.011253904179037E-4
56-57	1.877170310567101E-4	0.0012335690612298093	0.0	0.06249636298252327	2.078295700985005E-4
58-59	1.944212107373069E-4	0.0012335690612298093	0.0	0.06781277746923653	2.1453374977909728E-4
60-61	2.011253904179037E-4	0.0012670899596327933	0.0	0.07362530125231395	2.1453374977909728E-4
62-63	2.2794210914029085E-4	0.0012872024986745837	0.0	0.07886796976254064	2.5475882786267804E-4
64-65	2.2794210914029085E-4	0.00144139863132831	0.0	0.08405030065564195	2.5475882786267804E-4
66-67	2.2794210914029085E-4	0.0014481028110089067	0.0	0.08903821033800596	2.614630075432748E-4
68-69	2.2794210914029085E-4	0.0015017362484536808	0.0	0.09448870841833115	2.815755465850652E-4
70-71	2.2794210914029085E-4	0.0015352571468566648	0.0	0.0994028721242086	2.949839059462588E-4
72-73	2.2794210914029085E-4	0.0015553696858984552	0.0	0.10303653751109207	2.949839059462588E-4
74-75	2.2794210914029085E-4	0.0015821864046208424	0.0	0.10583218043790092	3.0839226530745233E-4
76-77	2.2794210914029085E-4	0.0016492282014268103	0.0	0.10853396484918143	3.285048043492427E-4
78-79	2.4135046850148445E-4	0.0016626365607880038	0.0	0.11039102262070674	3.352089840298395E-4
80-81	2.4135046850148445E-4	0.0016626365607880038	0.0	0.11104132804972464	3.486173433910331E-4
82-83	2.4135046850148445E-4	0.0016626365607880038	0.0	0.11141676211183804	3.6202570275222667E-4
84-85	2.4805464818208124E-4	0.0016693407404686006	0.0	0.11162459168193656	3.6202570275222667E-4
86-87	2.5475882786267804E-4	0.001729678357593972	0.0	0.1117251543771455	3.6872988243282347E-4
88	2.5475882786267804E-4	0.0017967201543999398	0.0	0.1117318585568261	3.754340621134202E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10025	0.0	51.975166	1
CTTATAC	6080	0.0	38.033848	1
GTATCAA	18155	0.0	36.846367	1
TATACAC	8295	0.0	30.763414	3
TCAACGC	22450	0.0	29.968496	4
ATCAACG	22905	0.0	29.279875	3
TATCAAC	23175	0.0	29.03097	2
CAACGCA	23335	0.0	28.776962	5
TTATACA	8540	0.0	28.22915	2
AACGCAG	24010	0.0	28.055456	6
GTGGTAT	4640	0.0	24.41206	1
TACACAT	10290	0.0	24.383682	5
ACGCAGA	27575	0.0	24.366177	7
CGCAGAG	28290	0.0	23.750347	8
TGGTATC	4795	0.0	22.998934	2
GTACATG	26375	0.0	22.721243	1
TACATGG	27315	0.0	22.033222	2
ACACATC	10775	0.0	21.896519	6
ACATGGG	27520	0.0	21.341005	3
ACATCTC	11770	0.0	20.590029	8
>>END_MODULE