FastQCFastQC Report
Fri 27 May 2016
SRR2138754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7636511
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100600.050.6122361
GTATCAA187800.037.9930571
CTTATAC63600.032.94061
TCAACGC231000.030.6648454
ATCAACG230200.030.622223
CAACGCA234300.030.1230095
TATCAAC239100.029.7876072
AACGCAG243650.028.9833356
TATACAC93900.026.3802573
ACGCAGA278950.025.4077827
CGCAGAG280150.025.2070088
TTATACA95850.023.828042
ACCGAAC10050.023.0660788
TGGTATC46000.023.0519642
GTGGTAT51400.022.1335741
GCAGAGT324550.021.8247059
ACATCTC108050.020.818658
GAGTACT197950.020.64560112-13
ACACATC111500.020.4056246
TACACAT125450.019.7115445
Produced by FastQC (version 0.11.5)