##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138754_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7636511
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.47225008907864	28.0	18.0	32.0	18.0	33.0
2	31.639800820034175	33.0	32.0	33.0	27.0	33.0
3	32.00118797707487	33.0	33.0	33.0	29.0	34.0
4	32.66066231031422	33.0	33.0	34.0	31.0	34.0
5	33.01038262106871	34.0	33.0	34.0	32.0	34.0
6	37.08128293143295	38.0	38.0	38.0	36.0	38.0
7	37.14794308552688	38.0	38.0	38.0	36.0	38.0
8	37.23296961138405	38.0	38.0	38.0	36.0	38.0
9	37.323829036584904	38.0	38.0	38.0	37.0	38.0
10-11	37.34357961377912	38.0	38.0	38.0	37.0	38.0
12-13	37.36643880955583	38.0	38.0	38.0	37.0	38.0
14-15	37.36802245161435	38.0	38.0	38.0	37.0	38.0
16-17	37.357231856275725	38.0	38.0	38.0	37.0	38.0
18-19	37.241375544407646	38.0	38.0	38.0	37.0	38.0
20-21	37.355800751695114	38.0	38.0	38.0	37.0	38.0
22-23	37.373684923406884	38.0	38.0	38.0	37.0	38.0
24-25	37.38094218481399	38.0	38.0	38.0	37.0	38.0
26-27	37.351956712013276	38.0	38.0	38.0	37.0	38.0
28-29	37.32604117730226	38.0	38.0	38.0	37.0	38.0
30-31	37.327337038385025	38.0	38.0	38.0	37.0	38.0
32-33	37.31117866301834	38.0	38.0	38.0	37.0	38.0
34-35	37.28986210303835	38.0	38.0	38.0	37.0	38.0
36-37	37.26405632048634	38.0	38.0	38.0	37.0	38.0
38-39	37.22259645999476	38.0	38.0	38.0	37.0	38.0
40-41	37.23404689566061	38.0	38.0	38.0	37.0	38.0
42-43	37.216030517065775	38.0	38.0	38.0	37.0	38.0
44-45	37.18212583576754	38.0	38.0	38.0	37.0	38.0
46-47	37.14380216797371	38.0	38.0	38.0	36.0	38.0
48-49	37.10909560964109	38.0	38.0	38.0	36.0	38.0
50-51	37.08037106725935	38.0	38.0	38.0	36.0	38.0
52-53	37.059516728099396	38.0	38.0	38.0	36.0	38.0
54-55	37.04513207593382	38.0	38.0	38.0	36.0	38.0
56-57	37.031959077398156	38.0	38.0	38.0	36.0	38.0
58-59	37.02128863059285	38.0	38.0	38.0	36.0	38.0
60-61	37.016770491733524	38.0	38.0	38.0	36.0	38.0
62-63	37.00944937139268	38.0	38.0	38.0	36.0	38.0
64-65	37.015055533538536	38.0	38.0	38.0	36.0	38.0
66-67	37.0078645864331	38.0	38.0	38.0	36.0	38.0
68-69	37.00933922960051	38.0	38.0	38.0	36.0	38.0
70-71	36.99940606912463	38.0	38.0	38.0	36.0	38.0
72-73	36.96931786379978	38.0	38.0	38.0	36.0	38.0
74-75	36.98953871029961	38.0	38.0	38.0	36.0	38.0
76-77	36.97749664158627	38.0	38.0	38.0	36.0	38.0
78-79	36.97014125356588	38.0	38.0	38.0	36.0	38.0
80-81	36.94699835240642	38.0	38.0	38.0	36.0	38.0
82-83	36.93960548391064	38.0	38.0	38.0	36.0	38.0
84-85	36.922416108367855	38.0	38.0	38.0	35.5	38.0
86-87	36.88568486503173	38.0	38.0	38.0	35.0	38.0
88-89	36.87061711148895	38.0	38.0	38.0	35.0	38.0
90-91	36.8787161601554	38.0	38.0	38.0	35.0	38.0
92-93	36.84700895321692	38.0	38.0	38.0	35.0	38.0
94-95	36.78847109288561	38.0	38.0	38.0	35.0	38.0
96-97	36.80321500515279	38.0	38.0	38.0	35.0	38.0
98-99	36.82921841691439	38.0	38.0	38.0	35.0	38.0
100	35.24857077445148	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	12.0
19	36.0
20	91.0
21	240.0
22	657.0
23	1780.0
24	3968.0
25	7805.0
26	13868.0
27	22331.0
28	34397.0
29	49755.0
30	68123.0
31	89122.0
32	115682.0
33	154316.0
34	222261.0
35	381913.0
36	1061365.0
37	5408787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03465574789325	19.686542715645928	15.626259164689216	23.652542371771613
2	14.418128907298112	20.766302831227506	42.170449305972326	22.64511895550206
3	18.189589460422436	25.97636538466323	30.744969790523445	25.089075364390883
4	12.432156517551013	18.05594203949945	38.781742080905794	30.73015936204374
5	13.3713365286399	37.39141195882769	35.55988580707682	13.677365705455594
6	29.678648174750442	37.86238180106306	18.784022502484778	13.674947521701725
7	26.220141632743015	32.61623010822613	23.106219581167366	18.05740867786349
8	24.122953532051483	36.90832109061324	21.16308088864142	17.805644488693854
9	25.32507319114711	16.991581626740274	21.346410684146203	36.33693449796642
10-11	24.19923836945956	26.73736736580357	28.821925353083365	20.241468911653502
12-13	25.033632835311366	24.31022650986992	28.91258383000979	21.743556824808923
14-15	22.181485902494337	25.517256867518224	26.71719629011183	25.584060939875613
16-17	21.659400477521736	28.697817629019323	27.409133568981957	22.233648324476977
18-19	21.71305063267767	27.625515107619176	29.197659768970414	21.463774490732746
20-21	22.47568586301322	26.747717936761045	29.233181966216808	21.543414234008925
22-23	22.178997065932094	26.85648761381226	29.055077435649924	21.909437884605726
24-25	22.03571335255147	26.967560706497895	29.191749373815007	21.80497656713563
26-27	21.981446331096656	27.10662786157328	29.131477774329927	21.780448033000134
28-29	21.71480084684273	27.049667660415146	29.247691672199927	21.98783982054219
30-31	22.16272682320339	26.82604920147098	29.295079846344496	21.716144128981128
32-33	21.788005470690944	27.145781301115292	29.136099859816827	21.930113368376936
34-35	21.93479848458241	27.185346301443573	29.28419979560286	21.595655418371155
36-37	22.06443381753529	27.008585277135484	29.05918771081677	21.86779319451246
38-39	21.81870307018517	27.048392052906934	29.194551162955367	21.93835371395253
40-41	22.083933717458912	26.92929835114092	29.092906023523902	21.89386190787627
42-43	22.08811681559215	26.930696808897075	29.020606684767973	21.9605796907428
44-45	22.013208610012786	27.14507274399155	28.88660696860018	21.955111677395482
46-47	22.197069192543626	27.030424097638	28.489318771519024	22.28318793829935
48-49	22.09713282283302	27.116545037790168	28.519718090021577	22.266604049355227
50-51	22.015075313835663	27.29004878033226	28.42331410268097	22.27156180315111
52-53	22.159944811086035	27.308431863025156	28.43255274696369	22.09907057892512
54-55	22.13261918367255	27.224021828874044	28.311697277633673	22.33166170981973
56-57	22.232289840302407	27.42624578450958	28.146708522836057	22.194755852351957
58-59	22.017038012167195	27.670458998714125	28.3647554247775	21.94774756434118
60-61	22.223241487242447	27.805760799556566	28.050578664332093	21.92041904886889
62-63	22.068982124669635	27.98119581917198	27.957144061181406	21.992677994976983
64-65	22.005309801942357	27.87415680185746	28.02511329963746	22.095420096562727
66-67	22.117540174442098	27.828508775262833	27.931703761994747	22.12224728830032
68-69	22.115183375582372	27.6992017423845	28.03466082712748	22.150954054905647
70-71	22.132166032890996	27.423927536320253	28.11606371223852	22.327842718550233
72-73	22.2681222069335	27.265420466860686	28.145487561340914	22.3209697648649
74-75	22.140060845955453	27.414902062054708	28.087944521802854	22.357092570186985
76-77	22.25046611720016	27.388322578271872	27.99313844503689	22.368072859491075
78-79	22.228216186971892	27.33898733902173	28.006617920674522	22.42617855333186
80-81	22.234309716533225	27.419313476789892	27.959605272076328	22.386771534600555
82-83	22.33009654601959	27.388785520502708	28.013138007708577	22.267979925769126
84-85	22.422351341470826	27.379184349469526	27.91150919366419	22.28695511539546
86-87	22.216391658564355	27.430024177956767	27.976814600027026	22.376769563451845
88-89	22.32397080399097	27.394116474457913	27.902381639162527	22.37953108238859
90-91	22.356027746117256	27.533993807854905	27.815559474760377	22.29441897126746
92-93	22.415941129400903	27.504721357010286	27.81287225361067	22.26646525997814
94-95	22.311377372917534	27.55442410986885	27.798588745629726	22.33560977158389
96-97	22.382275504741724	27.594816269169296	27.909325426673746	22.11358279941523
98-99	23.467450698877986	29.523279488811788	28.13489939855528	18.874370413754946
100	22.383506949298244	30.40128582888239	22.05119893454977	25.164008287269596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1947.0
1	1833.5
2	2250.5
3	3035.0
4	3945.5
5	5484.5
6	6896.5
7	8985.0
8	11407.0
9	12555.5
10	12327.0
11	11883.5
12	12810.0
13	15677.5
14	21256.0
15	27398.0
16	31406.0
17	33102.5
18	32429.5
19	29766.0
20	26572.5
21	24392.5
22	24664.0
23	28280.0
24	35277.5
25	45513.5
26	59193.0
27	75038.0
28	89916.0
29	105603.5
30	122084.0
31	138314.5
32	156202.0
33	173897.5
34	191551.5
35	208291.5
36	225636.5
37	242223.5
38	253654.5
39	262472.0
40	272445.5
41	281598.0
42	290130.5
43	306203.5
44	319632.0
45	325272.5
46	325993.0
47	321354.0
48	317865.0
49	314100.5
50	307918.5
51	300192.5
52	289096.0
53	272815.5
54	255679.5
55	238496.0
56	219471.0
57	198310.5
58	176648.5
59	154453.5
60	129793.5
61	104859.0
62	82149.5
63	63167.5
64	47576.0
65	35132.5
66	25693.0
67	19336.0
68	14528.0
69	10471.5
70	7647.5
71	5648.5
72	4076.5
73	2736.0
74	1849.0
75	1200.0
76	694.0
77	405.5
78	258.0
79	156.0
80	94.0
81	57.0
82	35.5
83	25.5
84	22.0
85	18.5
86	18.0
87	16.0
88	11.5
89	8.5
90	5.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004072540457284747
6	2.749946932571694E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.547492696599272E-6
14-15	6.547492696599272E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.627612635243222E-5
34-35	1.2510601088290606E-4
36-37	1.1233196129754252E-4
38-39	1.3284903659871393E-4
40-41	1.068981581915023E-4
42-43	1.344121340822867E-4
44-45	1.6241399164055185E-4
46-47	2.0468720043175354E-5
48-49	3.0267540985861967E-4
50-51	3.8818376924637657E-4
52-53	3.699910971764862E-4
54-55	0.001982511994549076
56-57	0.003123602878304432
58-59	0.0018773097423310293
60-61	0.0033023096094403747
62-63	0.001842290102956455
64-65	0.0024642068471314293
66-67	6.418750424227758E-4
68-69	5.51346735181681E-4
70-71	3.387351809003942E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	8.189898857206083E-6
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	309.0
22-23	680.0
24-25	756.0
26-27	1872.0
28-29	4585.0
30-31	12984.0
32-33	16682.0
34-35	23256.0
36-37	36831.0
38-39	44005.0
40-41	43220.0
42-43	48625.0
44-45	58846.0
46-47	60997.0
48-49	56612.0
50-51	51659.0
52-53	49464.0
54-55	52338.0
56-57	54189.0
58-59	54890.0
60-61	56375.0
62-63	55342.0
64-65	58645.0
66-67	64858.0
68-69	67390.0
70-71	76375.0
72-73	57556.0
74-75	56273.0
76-77	58697.0
78-79	60096.0
80-81	60650.0
82-83	57195.0
84-85	56106.0
86-87	58078.0
88-89	60135.0
90-91	60633.0
92-93	60821.0
94-95	72432.0
96-97	233030.0
98-99	518125.0
100-101	5114833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.18449967192925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96731870148503	76.63821030411006
2	6.9968901085680075	11.920531663156728
3	1.6679293108639557	4.262451715022754
4	0.6252961188077643	2.130621481097545
5	0.29748308697741743	1.2670473962516158
6	0.1452170096352451	0.7422142985779249
7	0.08982919556599657	0.5356438556154913
8	0.050919153466761465	0.3470018089427405
9	0.03279173816919429	0.2514013028384149
>10	0.12343741407250576	1.6264568527520267
>50	0.002289273141204285	0.12909940959583585
>100	4.990985548198607E-4	0.07704339193564438
>500	7.484302388154514E-5	0.04851729122673942
>1k	2.4947668276515706E-5	0.0237592288764997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.2379941572794174E-5	0.0	0.0	0.0
3	0.0	5.2379941572794174E-5	0.0	0.0	0.0
4	0.0	6.547492696599271E-5	0.0	0.0	0.0
5	0.0	6.547492696599271E-5	0.0	0.0	0.0
6	9.16648977523898E-5	6.547492696599271E-5	0.0	0.0	0.0
7	1.0475988314558835E-4	6.547492696599271E-5	0.0	0.0	0.0
8	1.0475988314558835E-4	1.0475988314558835E-4	0.0	0.0	0.0
9	1.0475988314558835E-4	1.4404483932518397E-4	0.0	0.0	2.6189970786397087E-5
10-11	1.0475988314558835E-4	1.4404483932518397E-4	0.0	0.0	2.6189970786397087E-5
12-13	1.0475988314558835E-4	1.4404483932518397E-4	0.0	0.0	3.273746348299636E-5
14-15	1.1785486853878688E-4	2.553522151673716E-4	0.0	0.0	8.511740505579054E-5
16-17	1.9642478089797815E-4	2.6189970786397084E-4	0.0	0.0	1.0475988314558835E-4
18-19	1.9642478089797815E-4	2.749946932571694E-4	0.0	0.0	1.1785486853878688E-4
20-21	2.0297227359457743E-4	2.8808967865036794E-4	0.0	1.3094985393198544E-5	1.2440236123538616E-4
22-23	2.2261475168437522E-4	3.3392212752656283E-4	0.0	1.9642478089797815E-5	1.4404483932518397E-4
24-25	2.3570973707757377E-4	3.6011209831296E-4	0.0	3.928495617959563E-5	1.636873174149818E-4
26-27	2.3570973707757377E-4	3.7975457640275777E-4	0.0	6.547492696599271E-5	1.7023481011158107E-4
28-29	2.3570973707757377E-4	3.8630206909935703E-4	0.0	4.3868201067215123E-4	1.7678230280818033E-4
30-31	2.3570973707757377E-4	4.452295033687505E-4	0.0	0.0011654536999946702	1.9642478089797815E-4
32-33	2.4225722977417306E-4	4.648719814585483E-4	0.0	0.002985656669649268	2.095197662911767E-4
34-35	2.749946932571694E-4	5.761793573007359E-4	0.0	0.005454061416267193	2.2261475168437525E-4
36-37	2.749946932571694E-4	6.809392404463243E-4	0.0	0.009159942282542381	2.2261475168437525E-4
38-39	2.749946932571694E-4	6.809392404463243E-4	0.0	0.015255657983076303	2.2261475168437525E-4
40-41	3.0118466404356647E-4	8.118890943783096E-4	0.0	0.021259708785857836	2.291622443809745E-4
42-43	3.0118466404356647E-4	8.315315724681075E-4	0.0	0.02732268702290876	2.3570973707757377E-4
44-45	3.077321567401658E-4	9.559339337034936E-4	0.0	0.03407969948579921	2.3570973707757377E-4
46-47	3.1427964943676504E-4	0.0010737888022422806	0.0	0.04219859042958231	2.3570973707757377E-4
48-49	3.1427964943676504E-4	0.0010803362949388798	0.0	0.04996391676774904	2.3570973707757377E-4
50-51	3.3392212752656283E-4	0.0012571185977470602	0.0	0.057310203573333426	2.3570973707757377E-4
52-53	3.4046962022316214E-4	0.0012964035539266559	0.0	0.06485946265251238	2.488047224707723E-4
54-55	3.4046962022316214E-4	0.0012964035539266559	0.0	0.07231050934124236	2.6189970786397084E-4
56-57	3.4046962022316214E-4	0.0013094985393198543	0.0	0.07942763390244575	2.6189970786397084E-4
58-59	3.4046962022316214E-4	0.0013094985393198543	0.0	0.08666261333218796	2.6189970786397084E-4
60-61	3.5356460561636067E-4	0.0013160460320164537	0.0	0.09338688833159542	2.6189970786397084E-4
62-63	3.928495617959563E-4	0.0013422360028028505	0.0	0.09999985595516067	3.0118466404356647E-4
64-65	3.928495617959563E-4	0.0015059233202178324	0.0	0.10631163891468237	3.0118466404356647E-4
66-67	3.928495617959563E-4	0.0015059233202178324	0.0	0.1122502147904979	3.0118466404356647E-4
68-69	3.928495617959563E-4	0.0015583032617906265	0.0	0.11796617591462907	3.0118466404356647E-4
70-71	3.928495617959563E-4	0.001591040725273623	0.0	0.12243156593370978	3.077321567401658E-4
72-73	4.321345179755519E-4	0.0015975882179702222	0.0	0.12637315653706255	3.1427964943676504E-4
74-75	4.452295033687505E-4	0.0016368731741498179	0.0	0.12928679078704922	3.1427964943676504E-4
76-77	4.5177699606534975E-4	0.001689253115722612	0.0	0.1322135200224291	3.1427964943676504E-4
78-79	4.58324488761949E-4	0.0017416330572954063	0.0	0.13420395780219527	3.1427964943676504E-4
80-81	4.58324488761949E-4	0.0017416330572954063	0.0	0.1347343047106198	3.3392212752656283E-4
82-83	4.58324488761949E-4	0.0017416330572954063	0.0	0.13525155663365113	3.666595910095592E-4
84-85	4.58324488761949E-4	0.0017481805499920055	0.0	0.13540869645836953	3.666595910095592E-4
86-87	4.714194741551476E-4	0.001780918013475002	0.0	0.13550690884881852	3.666595910095592E-4
88	4.845144595483461E-4	0.0018463929404409945	0.0	0.13552000383421173	3.666595910095592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10060	0.0	50.612236	1
GTATCAA	18780	0.0	37.993057	1
CTTATAC	6360	0.0	32.9406	1
TCAACGC	23100	0.0	30.664845	4
ATCAACG	23020	0.0	30.62222	3
CAACGCA	23430	0.0	30.123009	5
TATCAAC	23910	0.0	29.787607	2
AACGCAG	24365	0.0	28.983335	6
TATACAC	9390	0.0	26.380257	3
ACGCAGA	27895	0.0	25.407782	7
CGCAGAG	28015	0.0	25.207008	8
TTATACA	9585	0.0	23.82804	2
ACCGAAC	1005	0.0	23.066078	8
TGGTATC	4600	0.0	23.051964	2
GTGGTAT	5140	0.0	22.133574	1
GCAGAGT	32455	0.0	21.824705	9
ACATCTC	10805	0.0	20.81865	8
GAGTACT	19795	0.0	20.645601	12-13
ACACATC	11150	0.0	20.405624	6
TACACAT	12545	0.0	19.711544	5
>>END_MODULE