FastQCFastQC Report
Fri 27 May 2016
SRR2138753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825368
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86900.049.711041
GTATCAA155250.037.2491951
TCAACGC190450.030.2964154
ATCAACG190700.030.1886023
CAACGCA196000.029.3943655
TATCAAC200450.028.9577482
AACGCAG202150.028.5202586
ACGCAGA230950.024.9637177
CGCAGAG236450.024.4013488
GTGGTAT42600.023.0652581
GTACATG180800.021.3314971
GCAGAGT274150.021.0615629
TGGTATC42350.020.9528222
TACATGG181750.020.6960492
ACATGGG183150.019.7342933
GAGTACT178500.019.61703312-13
CAGAGTA268550.018.93806810-11
AGAGTAC250800.018.81143810-11
GTACTTT196650.017.72961614-15
CATGGGG134400.017.3914814
Produced by FastQC (version 0.11.5)