##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6825368
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.683964000182847	25.0	18.0	31.0	18.0	33.0
2	31.472362369325726	32.0	31.0	33.0	27.0	33.0
3	31.89843038499902	33.0	31.0	33.0	29.0	34.0
4	32.62104856470743	33.0	33.0	34.0	31.0	34.0
5	32.99259160824735	34.0	33.0	34.0	32.0	34.0
6	37.06787575409853	38.0	38.0	38.0	36.0	38.0
7	37.11670564869176	38.0	38.0	38.0	36.0	38.0
8	37.206162363699654	38.0	38.0	38.0	36.0	38.0
9	37.30142037762653	38.0	38.0	38.0	37.0	38.0
10-11	37.32688794215931	38.0	38.0	38.0	37.0	38.0
12-13	37.351113522377105	38.0	38.0	38.0	37.0	38.0
14-15	37.35403124051333	38.0	38.0	38.0	37.0	38.0
16-17	37.34474558734415	38.0	38.0	38.0	37.0	38.0
18-19	37.231406204031785	38.0	38.0	38.0	36.5	38.0
20-21	37.342456407863494	38.0	38.0	38.0	37.0	38.0
22-23	37.3609795335298	38.0	38.0	38.0	37.0	38.0
24-25	37.367814926679415	38.0	38.0	38.0	37.0	38.0
26-27	37.33912230568514	38.0	38.0	38.0	37.0	38.0
28-29	37.31409547733371	38.0	38.0	38.0	37.0	38.0
30-31	37.31668127009844	38.0	38.0	38.0	37.0	38.0
32-33	37.30361926228511	38.0	38.0	38.0	37.0	38.0
34-35	37.28410114534019	38.0	38.0	38.0	37.0	38.0
36-37	37.259252728877044	38.0	38.0	38.0	37.0	38.0
38-39	37.22587931710498	38.0	38.0	38.0	37.0	38.0
40-41	37.22410086762073	38.0	38.0	38.0	37.0	38.0
42-43	37.20169017091278	38.0	38.0	38.0	37.0	38.0
44-45	37.16871394839879	38.0	38.0	38.0	36.5	38.0
46-47	37.131461104204945	38.0	38.0	38.0	36.0	38.0
48-49	37.09772343884393	38.0	38.0	38.0	36.0	38.0
50-51	37.06829884388871	38.0	38.0	38.0	36.0	38.0
52-53	37.04600353044803	38.0	38.0	38.0	36.0	38.0
54-55	37.029834119120416	38.0	38.0	38.0	36.0	38.0
56-57	37.017194438261185	38.0	38.0	38.0	36.0	38.0
58-59	37.00350767316894	38.0	38.0	38.0	36.0	38.0
60-61	37.0011064503201	38.0	38.0	38.0	36.0	38.0
62-63	36.99559199430176	38.0	38.0	38.0	36.0	38.0
64-65	36.99987943061839	38.0	38.0	38.0	36.0	38.0
66-67	36.99233829448487	38.0	38.0	38.0	36.0	38.0
68-69	36.99413149070605	38.0	38.0	38.0	36.0	38.0
70-71	36.98495333292121	38.0	38.0	38.0	36.0	38.0
72-73	36.95701584357029	38.0	38.0	38.0	36.0	38.0
74-75	36.97617146821415	38.0	38.0	38.0	36.0	38.0
76-77	36.9640907030514	38.0	38.0	38.0	36.0	38.0
78-79	36.95244785478056	38.0	38.0	38.0	36.0	38.0
80-81	36.92868602190575	38.0	38.0	38.0	35.5	38.0
82-83	36.91751415353673	38.0	38.0	38.0	35.5	38.0
84-85	36.8979896491679	38.0	38.0	38.0	35.0	38.0
86-87	36.86075052614689	38.0	38.0	38.0	35.0	38.0
88-89	36.8439988876232	38.0	38.0	38.0	35.0	38.0
90-91	36.851461017130035	38.0	38.0	38.0	35.0	38.0
92-93	36.81496112918112	38.0	38.0	38.0	35.0	38.0
94-95	36.75889266994044	38.0	38.0	38.0	35.0	38.0
96-97	36.770687003009805	38.0	38.0	38.0	35.0	38.0
98-99	36.79859801321007	38.0	38.0	38.0	35.0	38.0
100	35.19003112012419	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	10.0
19	30.0
20	82.0
21	223.0
22	604.0
23	1541.0
24	3425.0
25	7220.0
26	12410.0
27	19986.0
28	31227.0
29	45363.0
30	61813.0
31	81296.0
32	105285.0
33	141390.0
34	204479.0
35	352341.0
36	975716.0
37	4780924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32448243083743	19.472063630854773	15.825417765020141	23.378036173287654
2	14.595330244464474	20.74399504905816	41.98024194446365	22.680432762013712
3	18.493610893947405	25.65309299073691	30.442314612193805	25.41098150312188
4	12.570208668602193	17.75754801792372	38.82406047556703	30.848182837907057
5	13.577569597210747	37.187288371761824	35.47490731946893	13.760234711558494
6	30.09274249672178	37.710183360560265	18.66365827393467	13.533415868783285
7	26.44946030748818	32.492885365301916	23.08401539667898	17.973638930530925
8	24.085675673458194	37.05401672114969	21.060901038596015	17.799406566796105
9	25.200252938742647	17.12842443074132	21.472527195603224	36.19879543491281
10-11	24.203156518447063	26.813638473412716	28.78089503745439	20.202309970685832
12-13	24.99621265842369	24.275702057383572	29.045774528201264	21.68231075599147
14-15	22.11804141813913	25.62361848088169	26.743624188999654	25.51471591197953
16-17	21.69899850088669	28.761086581705193	27.413254494116657	22.12666042329146
18-19	21.677541782362503	27.70157594433004	29.28373971923565	21.337142554071807
20-21	22.40667958433909	26.93081231159184	29.303222214489526	21.359285889579542
22-23	22.143303508960866	26.86359104199222	29.176229000777294	21.816876448269625
24-25	21.94455749779378	26.95203245093108	29.288503884741026	21.81490616653412
26-27	21.91081578828202	27.12818332642361	29.17870006077331	21.782300824521055
28-29	21.81584080085367	27.123089796924443	29.277727257701958	21.783342144519928
30-31	22.081795844573694	27.042376522775864	29.152466610460582	21.723361022189863
32-33	21.96879741965099	27.092114410049035	29.112717763967886	21.82637040633209
34-35	22.02282587592291	27.137226566178406	29.06743427821835	21.772513279680336
36-37	21.983908565553428	27.12543430475785	29.060114278285944	21.830542851402775
38-39	21.8189190815838	27.200431201939786	29.172743709356357	21.807906007120057
40-41	22.09676563905787	27.057541880313064	29.01973313536518	21.825959345263886
42-43	22.00154693842228	27.14545465726105	28.883765590684053	21.969232813632615
44-45	22.052835865676972	27.289100402995647	28.738930900931166	21.91913283039621
46-47	22.108984673930447	27.23306947279945	28.50463133243614	22.153314520833963
48-49	22.05457074673989	27.307063135983267	28.50376074656297	22.13460537071387
50-51	22.05345779579102	27.485083215167727	28.39652526024069	22.064933728800565
52-53	22.177362958299124	27.489091507154846	28.27817110806754	22.055374426478487
54-55	22.16050395354178	27.392709109628953	28.28548618174262	22.16130075508664
56-57	22.188802716647878	27.501709586672956	28.202590956972852	22.106896739706315
58-59	22.11962151885314	27.564919418164553	28.29359245370449	22.02186660927782
60-61	22.215317718138827	27.54798949621386	28.266225544685554	21.970467240961764
62-63	22.15365960548807	27.638434896382513	28.212565855098642	21.99533964303077
64-65	22.13781705583033	27.583087980462462	28.20104521942482	22.07804974428239
66-67	22.18336171772334	27.565422353439555	28.110306344945542	22.14090958389156
68-69	22.141533899615524	27.52852526465835	28.211696393368314	22.118244442357813
70-71	22.158389846941756	27.341504563590096	28.23870790367053	22.26139768579762
72-73	22.24971149107075	27.333522686071305	28.174322529453367	22.24244329340458
74-75	22.105511998692844	27.49116690680707	28.12903000382154	22.274291090678545
76-77	22.25424375878537	27.4183348008768	28.043551709870357	22.28386973046747
78-79	22.176168275184338	27.440208219293748	27.98941741280268	22.394206092719234
80-81	22.21238885958022	27.485638509968247	27.971234254475956	22.330738375975574
82-83	22.26147295930152	27.494809480083465	28.04812436114579	22.195593199469226
84-85	22.38195177572977	27.450894918772	27.94577649275537	22.221376812742857
86-87	22.27188510609047	27.477970976342814	28.011802572629747	22.23834134493697
88-89	22.292351651529692	27.497488519490194	27.94235631477987	22.267803514200246
90-91	22.301622087246972	27.542025235804036	27.9094931362798	22.246859540669195
92-93	22.34870592419108	27.496356586614105	27.966919650298035	22.188017838896776
94-95	22.233193301516998	27.63213188646592	27.88071409313076	22.25396071888632
96-97	22.34069641755541	27.708121392842592	27.974667292197953	21.976514897404048
98-99	23.427053629204075	29.60394180406432	28.15429032522689	18.814714241504717
100	22.327890116942292	30.481827134379024	22.168134748475847	25.022148000202836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1644.0
1	1514.0
2	1914.0
3	2644.0
4	3258.0
5	4318.0
6	5502.0
7	7634.0
8	9953.5
9	10890.5
10	10489.0
11	10083.5
12	11122.0
13	13463.0
14	17755.0
15	22691.5
16	26059.5
17	27457.5
18	26918.0
19	24920.5
20	22851.5
21	21874.5
22	22917.5
23	26550.0
24	32592.5
25	41927.0
26	54386.0
27	67646.5
28	80783.0
29	95910.0
30	111021.0
31	125274.5
32	140364.0
33	155196.0
34	170019.0
35	183948.5
36	198390.5
37	212667.5
38	223906.5
39	232426.0
40	242067.0
41	252549.0
42	260603.5
43	268853.0
44	274213.0
45	277756.0
46	280719.5
47	280795.5
48	280391.5
49	277807.5
50	272775.5
51	264348.5
52	253098.0
53	240412.0
54	225549.0
55	209533.5
56	192971.5
57	175563.0
58	156456.5
59	136233.5
60	114907.5
61	93387.0
62	73765.5
63	57054.5
64	43177.5
65	32126.5
66	24036.0
67	18095.0
68	13509.5
69	10066.0
70	7393.0
71	5274.5
72	3753.5
73	2547.0
74	1665.5
75	1039.0
76	620.0
77	355.0
78	220.0
79	147.5
80	103.0
81	62.5
82	36.0
83	22.5
84	16.5
85	12.0
86	8.0
87	7.0
88	5.5
89	3.0
90	2.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004541879646635903
6	2.6372204399821374E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.465122466656743E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.612206088357734E-5
34-35	1.2516350403033846E-4
36-37	1.6988082417017096E-4
38-39	1.7810870374934405E-4
40-41	7.459606417738109E-5
42-43	1.724816867569086E-4
44-45	1.5092316682684646E-4
46-47	6.842194291709351E-5
48-49	4.365505886310279E-4
50-51	3.084918711620719E-4
52-53	4.579302932298421E-4
54-55	0.0021872505167379345
56-57	0.0034611393110932586
58-59	0.0020522008586440084
60-61	0.0036317170702435545
62-63	0.0020822677455439873
64-65	0.002874513711227972
66-67	8.079060543990077E-4
68-69	6.418961150389758E-4
70-71	3.818472474873206E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	9.127929483458685E-6
94-95	9.217672194873389E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	49.0
20-21	252.0
22-23	495.0
24-25	613.0
26-27	1409.0
28-29	3468.0
30-31	10501.0
32-33	13328.0
34-35	18850.0
36-37	30121.0
38-39	35045.0
40-41	34570.0
42-43	39246.0
44-45	48010.0
46-47	49141.0
48-49	46383.0
50-51	41866.0
52-53	40544.0
54-55	43834.0
56-57	45942.0
58-59	45999.0
60-61	45685.0
62-63	43650.0
64-65	46919.0
66-67	50552.0
68-69	52339.0
70-71	53373.0
72-73	47363.0
74-75	47101.0
76-77	49250.0
78-79	51058.0
80-81	50597.0
82-83	48159.0
84-85	47737.0
86-87	48477.0
88-89	51045.0
90-91	51821.0
92-93	51912.0
94-95	62284.0
96-97	210548.0
98-99	472406.0
100-101	4693426.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.57540942880829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5772809939394	78.41765186998518
2	6.70488636037283	11.609565636458202
3	1.582012747699664	4.108902040610772
4	0.5452311943005955	1.8881445551972873
5	0.24617517717593157	1.0656358377607846
6	0.11492310364153259	0.5969708850357032
7	0.07124046877597652	0.43173709265262794
8	0.04134445847601384	0.2863530736138609
9	0.028109433939485718	0.21902271769107143
>10	0.08669098280052111	1.1713327387430779
>50	0.0016852545457544195	0.09455388971534057
>100	3.4303953551825307E-4	0.05818916630013717
>500	7.678479683950181E-5	0.05194049623594885
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.930244933313486E-5	0.0	1.465122466656743E-5	0.0
3	0.0	2.930244933313486E-5	0.0	1.465122466656743E-5	0.0
4	0.0	7.325612333283714E-5	0.0	2.930244933313486E-5	0.0
5	0.0	7.325612333283714E-5	0.0	2.930244933313486E-5	0.0
6	5.860489866626972E-5	7.325612333283714E-5	0.0	2.930244933313486E-5	0.0
7	5.860489866626972E-5	7.325612333283714E-5	0.0	2.930244933313486E-5	0.0
8	5.860489866626972E-5	7.325612333283714E-5	0.0	2.930244933313486E-5	0.0
9	5.860489866626972E-5	1.1720979733253944E-4	0.0	4.3953673999702286E-5	0.0
10-11	5.860489866626972E-5	1.1720979733253944E-4	0.0	5.860489866626972E-5	0.0
12-13	8.058173566612086E-5	1.1720979733253944E-4	0.0	5.860489866626972E-5	0.0
14-15	9.523296033268829E-5	1.6848908366552543E-4	0.0	7.325612333283714E-5	0.0
16-17	1.3186102199910687E-4	1.8314030833209286E-4	0.0	7.325612333283714E-5	0.0
18-19	1.3186102199910687E-4	2.2709398233179516E-4	0.0	7.325612333283714E-5	0.0
20-21	1.3186102199910687E-4	2.783732686647812E-4	0.0	7.325612333283714E-5	0.0
22-23	1.3186102199910687E-4	3.0035010566463227E-4	0.0	7.325612333283714E-5	0.0
24-25	1.4651224666567428E-4	3.223269426644834E-4	0.0	9.523296033268829E-5	0.0
26-27	1.4651224666567428E-4	3.223269426644834E-4	0.0	1.1720979733253944E-4	0.0
28-29	1.4651224666567428E-4	3.223269426644834E-4	0.0	3.223269426644834E-4	0.0
30-31	1.611634713322417E-4	3.8093184133075316E-4	0.0	0.0010109345019931526	0.0
32-33	1.611634713322417E-4	3.955830659973206E-4	0.0	0.0026298948276488538	0.0
34-35	1.611634713322417E-4	4.6151357699687404E-4	0.0	0.00479095046596755	0.0
36-37	1.611634713322417E-4	5.347697003297111E-4	0.0	0.008021545504945667	0.0
38-39	1.611634713322417E-4	5.420953126629949E-4	0.0	0.012644006887247691	0.0
40-41	1.611634713322417E-4	6.007002113292645E-4	0.0	0.01779391235754614	0.0
42-43	1.611634713322417E-4	6.080258236625482E-4	0.0	0.022892538541511607	0.0
44-45	1.6848908366552543E-4	6.886075593286691E-4	0.0	0.028965471165803808	0.0
46-47	1.7581469599880914E-4	8.058173566612086E-4	0.0	0.03541201001909347	0.0
48-49	1.7581469599880914E-4	8.131429689944923E-4	0.0	0.04204168918071524	0.0
50-51	1.9046592066537658E-4	9.010503169938969E-4	0.0	0.048488228034004904	0.0
52-53	2.05117145331944E-4	9.083759293271806E-4	0.0	0.054649068006296514	0.0
54-55	2.05117145331944E-4	9.083759293271806E-4	0.0	0.060780605529254975	0.0
56-57	2.1244275766522773E-4	9.230271539937481E-4	0.0	0.06587923171322044	0.0
58-59	2.2709398233179516E-4	9.376783786603155E-4	0.0	0.07073611269018755	0.0
60-61	2.783732686647812E-4	9.96283277326585E-4	0.0	0.07591532060981913	0.0
62-63	2.9302449333134856E-4	0.0010402369513262874	0.0	0.08064034056478714	0.0
64-65	3.0035010566463227E-4	0.0012233772596583802	0.0	0.08524082511008929	0.0
66-67	3.07675717997916E-4	0.0012453540966582315	0.0	0.08981200720605834	0.0
68-69	3.07675717997916E-4	0.0012746565459913661	0.0	0.09485935410369081	0.0
70-71	3.516293919976183E-4	0.0012893077706579337	0.0	0.0989616970103297	0.0
72-73	3.58955004330902E-4	0.0012893077706579337	0.0	0.10214101276297483	0.0
74-75	3.662806166641857E-4	0.0013479126693242034	0.0	0.10468300024262428	0.0
76-77	3.662806166641857E-4	0.0013991919556571894	0.0	0.10734952313193954	0.0
78-79	3.662806166641857E-4	0.0014211687926570407	0.0	0.10896115784526197	0.0
80-81	3.8093184133075316E-4	0.0014577968543234591	0.0	0.10950325315792496	0.0
82-83	3.8093184133075316E-4	0.0014870993036565942	0.0	0.10978895203892303	0.0
84-85	3.8093184133075316E-4	0.0014944249159898778	0.0	0.10991348744858885	0.0
86-87	3.8093184133075316E-4	0.0015090761406564453	0.0	0.1099794179595884	0.0
88	3.8093184133075316E-4	0.0015530298146561475	0.0	0.10998674357192169	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8690	0.0	49.71104	1
GTATCAA	15525	0.0	37.249195	1
TCAACGC	19045	0.0	30.296415	4
ATCAACG	19070	0.0	30.188602	3
CAACGCA	19600	0.0	29.394365	5
TATCAAC	20045	0.0	28.957748	2
AACGCAG	20215	0.0	28.520258	6
ACGCAGA	23095	0.0	24.963717	7
CGCAGAG	23645	0.0	24.401348	8
GTGGTAT	4260	0.0	23.065258	1
GTACATG	18080	0.0	21.331497	1
GCAGAGT	27415	0.0	21.061562	9
TGGTATC	4235	0.0	20.952822	2
TACATGG	18175	0.0	20.696049	2
ACATGGG	18315	0.0	19.734293	3
GAGTACT	17850	0.0	19.617033	12-13
CAGAGTA	26855	0.0	18.938068	10-11
AGAGTAC	25080	0.0	18.811438	10-11
GTACTTT	19665	0.0	17.729616	14-15
CATGGGG	13440	0.0	17.391481	4
>>END_MODULE