FastQCFastQC Report
Fri 27 May 2016
SRR2138752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9303175
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109750.047.0634571
GTATCAA204300.039.1477781
TCAACGC239450.032.6988564
ATCAACG239350.032.6584743
CAACGCA245700.031.8319825
TATCAAC253000.031.1861742
AACGCAG254100.030.9137656
ACGCAGA294900.026.56347
CGCAGAG302750.025.9031188
GTGGTAT55100.022.1445261
GCAGAGT354750.022.081889
GTACATG248850.020.9295941
TACATGG248750.020.5220222
GAGTACT214500.019.7071512-13
TGGTATC57600.019.6863332
ACATGGG251150.019.6735633
AGAGTAC318650.018.87399510-11
CAGAGTA343800.018.75984410-11
CATGGGG194700.017.5827224
GTACTTT241150.017.5743914-15
Produced by FastQC (version 0.11.5)