##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9303175
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.269396308249604	33.0	33.0	34.0	31.0	34.0
2	32.03520282054245	33.0	32.0	33.0	30.0	34.0
3	32.07104112305745	33.0	33.0	33.0	29.0	34.0
4	32.495469987396774	33.0	33.0	34.0	31.0	34.0
5	32.808006621395386	33.0	33.0	34.0	31.0	34.0
6	36.83244064526358	38.0	37.0	38.0	35.0	38.0
7	37.10373275790254	38.0	38.0	38.0	36.0	38.0
8	37.1454234710193	38.0	38.0	38.0	36.0	38.0
9	37.23301829751671	38.0	38.0	38.0	36.0	38.0
10-11	37.27631550519043	38.0	38.0	38.0	37.0	38.0
12-13	37.30996815603275	38.0	38.0	38.0	37.0	38.0
14-15	37.334605121369854	38.0	38.0	38.0	37.0	38.0
16-17	37.33053495177721	38.0	38.0	38.0	37.0	38.0
18-19	37.20335670349101	38.0	38.0	38.0	36.5	38.0
20-21	37.321388715334734	38.0	38.0	38.0	37.0	38.0
22-23	37.34138535275976	38.0	38.0	38.0	37.0	38.0
24-25	37.346257083623385	38.0	38.0	38.0	37.0	38.0
26-27	37.31565524882052	38.0	38.0	38.0	37.0	38.0
28-29	37.2954187083194	38.0	38.0	38.0	37.0	38.0
30-31	37.29767115361993	38.0	38.0	38.0	37.0	38.0
32-33	37.283200089714555	38.0	38.0	38.0	37.0	38.0
34-35	37.26814661361514	38.0	38.0	38.0	37.0	38.0
36-37	37.24584335364031	38.0	38.0	38.0	37.0	38.0
38-39	37.208453778822346	38.0	38.0	38.0	37.0	38.0
40-41	37.21048864762732	38.0	38.0	38.0	37.0	38.0
42-43	37.18629355640829	38.0	38.0	38.0	36.5	38.0
44-45	37.158259191416334	38.0	38.0	38.0	36.0	38.0
46-47	37.1249578340109	38.0	38.0	38.0	36.0	38.0
48-49	37.094425182756844	38.0	38.0	38.0	36.0	38.0
50-51	37.071181171548545	38.0	38.0	38.0	36.0	38.0
52-53	37.050597366474896	38.0	38.0	38.0	36.0	38.0
54-55	37.03428979085653	38.0	38.0	38.0	36.0	38.0
56-57	37.02005329944889	38.0	38.0	38.0	36.0	38.0
58-59	37.0086944202199	38.0	38.0	38.0	36.0	38.0
60-61	37.0042684402373	38.0	38.0	38.0	36.0	38.0
62-63	36.998314777535015	38.0	38.0	38.0	36.0	38.0
64-65	37.00184829771191	38.0	38.0	38.0	36.0	38.0
66-67	36.99153954087683	38.0	38.0	38.0	36.0	38.0
68-69	36.992779349294445	38.0	38.0	38.0	36.0	38.0
70-71	36.981149058440124	38.0	38.0	38.0	36.0	38.0
72-73	36.95231936819411	38.0	38.0	38.0	36.0	38.0
74-75	36.970027958744794	38.0	38.0	38.0	36.0	38.0
76-77	36.95635432828082	38.0	38.0	38.0	36.0	38.0
78-79	36.94254067799737	38.0	38.0	38.0	36.0	38.0
80-81	36.92052884160037	38.0	38.0	38.0	35.5	38.0
82-83	36.90677348114336	38.0	38.0	38.0	35.0	38.0
84-85	36.889219577885555	38.0	38.0	38.0	35.0	38.0
86-87	36.852682222965406	38.0	38.0	38.0	35.0	38.0
88-89	36.83661646991581	38.0	38.0	38.0	35.0	38.0
90-91	36.84283329992327	38.0	38.0	38.0	35.0	38.0
92-93	36.81079930763163	38.0	38.0	38.0	35.0	38.0
94-95	36.74866118535142	38.0	38.0	38.0	35.0	38.0
96-97	36.76124263760401	38.0	38.0	38.0	35.0	38.0
98-99	36.79439445804701	38.0	38.0	38.0	35.0	38.0
100	35.209903663771826	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	21.0
19	31.0
20	93.0
21	239.0
22	739.0
23	2149.0
24	5028.0
25	10053.0
26	17306.0
27	28453.0
28	42555.0
29	60870.0
30	83458.0
31	110496.0
32	143281.0
33	189664.0
34	269682.0
35	446796.0
36	1086463.0
37	6805794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.642568262985485	19.37356870100799	13.743060836757342	25.24080219924918
2	14.770763744635568	20.01230762615989	42.19289651113733	23.02403211806722
3	18.42234505961674	25.24255428926146	29.771191018120156	26.563909633001636
4	12.679144485619156	17.462844673995708	38.750770570262304	31.107240270122833
5	13.897538988332336	36.796059302661945	35.2515538736578	14.054847835347923
6	30.46280913205256	37.25154635895134	18.636377121540846	13.649267387455252
7	26.90281832845435	32.18142539309702	22.779859862881207	18.13589641556742
8	24.345559446103078	36.52097267868228	21.091208109059544	18.0422597661551
9	25.62343501009064	16.785086811760504	21.094497308714498	36.49698086943436
10-11	24.458800355792512	26.431642960602158	28.634627425583204	20.474929258022126
12-13	25.234162018253127	23.921459493286914	28.748914080291303	22.095464408168652
14-15	22.429269838444384	25.19401980442374	26.401011095783012	25.97569926134887
16-17	22.103115334281036	28.328807100801605	27.08933777984398	22.478739785073376
18-19	22.082880306991967	27.337946453764438	28.820585445291524	21.758587793952064
20-21	22.82956630184315	26.605505277470144	28.733896253449277	21.83103216723743
22-23	22.572747265670497	26.55847684741487	28.605538849056238	22.263237037858392
24-25	22.367922568870934	26.6914193370553	28.708159587318367	22.232498506755398
26-27	22.331216511959333	26.84042772227834	28.62209497043027	22.20626079533206
28-29	22.255556093285367	26.83592787967758	28.646899718767312	22.261616308269748
30-31	22.558060011876186	26.73651021148632	28.541025422724548	22.164404353912946
32-33	22.34406855481581	26.84506194583019	28.550409243458375	22.260460255895627
34-35	22.48052817285539	26.846906458664595	28.554168035734133	22.118397332745886
36-37	22.43937639333455	26.783229673580212	28.502929797624933	22.27446413546031
38-39	22.23675159539328	26.84413024674962	28.66094845023803	22.258169707619064
40-41	22.498535364309433	26.7296205270541	28.514931176092322	22.256912932544147
42-43	22.396986797156963	26.77953781895926	28.400535306377623	22.42294007750616
44-45	22.457681022631675	26.918995446602807	28.27668528655459	22.346638244210933
46-47	22.534354948715432	26.872021403088898	28.019494462008872	22.574129186186802
48-49	22.45464273444584	26.9552538058201	28.03018748908801	22.559915970646045
50-51	22.40569101494229	27.106825371883808	27.961770974144695	22.52571263902921
52-53	22.56859692041232	27.0742421155905	27.85499254584851	22.502168418148667
54-55	22.577963421412857	26.954312652672485	27.840622584514378	22.62710134140028
56-57	22.561589956217396	27.08976756261438	27.770608516827423	22.578033964340804
58-59	22.50537129796576	27.14241702813357	27.902068695730424	22.45014297817024
60-61	22.56138662831704	27.19181831881265	27.832578708793935	22.414216344076372
62-63	22.50634070179703	27.322593772746266	27.76246387370877	22.408601651747937
64-65	22.48845752874401	27.2560444628484	27.740382565807813	22.515115442599775
66-67	22.518088108378702	27.320556188664902	27.56578535200218	22.595570350954215
68-69	22.48066666743513	27.259584855021767	27.694704278531763	22.565044199011336
70-71	22.52326140427832	27.00868786601	27.731070466668186	22.736980263043492
72-73	22.60860841630389	26.976483165933452	27.6556883199856	22.759220097777057
74-75	22.493438482174618	27.103184079099613	27.64573918497244	22.757638253753328
76-77	22.68675002099954	27.034100734405392	27.508595978450202	22.770553266144873
78-79	22.586072855733143	27.091190018762784	27.471774934916475	22.850962190587598
80-81	22.657757982599417	27.109912141570902	27.444311172639125	22.788018703190556
82-83	22.683129004893324	27.10827097045944	27.52030117450483	22.68829885014241
84-85	22.785920504903494	27.110505084108528	27.414072100883153	22.68950231010483
86-87	22.681065998311126	27.146763407323636	27.469102132357186	22.70306846200805
88-89	22.733703111794625	27.119770052807052	27.41096378236057	22.73556305303775
90-91	22.731248658222412	27.192571919278663	27.37926685272649	22.696912569772433
92-93	22.82940160422408	27.161653664053492	27.39846543640465	22.610479295317774
94-95	22.70903166446444	27.24938772595184	27.416490763626783	22.625089845956936
96-97	22.7809821111984	27.317145578552903	27.465616374545483	22.436255935703212
98-99	23.875036486166604	29.1784318815015	27.719255833295186	19.227275799036715
100	22.738971380205612	30.071856712002575	21.62666210080289	25.56250980698892
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1842.0
1	1751.0
2	2387.0
3	3310.0
4	4091.0
5	5688.5
6	7277.0
7	9305.0
8	11847.5
9	13133.0
10	12766.0
11	12371.0
12	13420.5
13	16291.0
14	21585.5
15	27428.0
16	31633.5
17	33849.5
18	33549.5
19	31052.5
20	27957.0
21	25882.0
22	26528.0
23	30399.0
24	37582.0
25	48901.0
26	63645.5
27	80040.0
28	97479.0
29	116776.0
30	134925.5
31	153005.0
32	172938.5
33	193027.5
34	213417.5
35	233381.0
36	255110.5
37	276193.5
38	292416.0
39	304995.5
40	319679.0
41	333984.0
42	344792.5
43	356397.5
44	369527.5
45	384515.5
46	391816.0
47	394368.5
48	396298.0
49	393471.0
50	387616.0
51	377305.0
52	364717.5
53	349353.5
54	331827.5
55	311496.0
56	288481.0
57	264030.5
58	237036.0
59	207925.5
60	175386.5
61	142809.5
62	113909.5
63	88629.5
64	67244.5
65	50971.0
66	38718.5
67	29219.0
68	22030.0
69	16555.0
70	12349.5
71	8871.0
72	6491.5
73	4660.5
74	3179.0
75	2071.0
76	1331.0
77	793.5
78	452.5
79	248.5
80	168.0
81	122.5
82	80.5
83	57.5
84	41.5
85	24.5
86	15.5
87	13.5
88	8.0
89	3.0
90	3.0
91	2.5
92	1.5
93	1.5
94	1.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0049015524269940105
6	3.332195728877507E-4
7	1.0749018480250023E-5
8	0.0
9	0.0
10-11	0.0
12-13	1.0749018480250023E-5
14-15	1.0749018480250023E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.773666075081615E-5
34-35	1.512928705233993E-4
36-37	1.40959716640609E-4
38-39	1.3076418754657453E-4
40-41	9.861012318376587E-5
42-43	1.873084014098373E-4
44-45	1.5527332041681126E-4
46-47	2.235449126319418E-5
48-49	2.9286284241861454E-4
50-51	3.6871995464177296E-4
52-53	4.0536351887049963E-4
54-55	0.0022295740703293853
56-57	0.003587234098442151
58-59	0.0022438187020132417
60-61	0.003705547015962921
62-63	0.002100407650666826
64-65	0.0027664794980318346
66-67	6.914251794082999E-4
68-69	6.855475771838604E-4
70-71	3.1838834757427266E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	6.642646855636685E-6
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	79.0
20-21	331.0
22-23	723.0
24-25	843.0
26-27	1880.0
28-29	4914.0
30-31	15106.0
32-33	19725.0
34-35	27086.0
36-37	43397.0
38-39	49861.0
40-41	50092.0
42-43	56399.0
44-45	68207.0
46-47	70035.0
48-49	65075.0
50-51	58318.0
52-53	55201.0
54-55	59582.0
56-57	61164.0
58-59	64297.0
60-61	64362.0
62-63	63955.0
64-65	68118.0
66-67	73729.0
68-69	75097.0
70-71	79334.0
72-73	67960.0
74-75	68183.0
76-77	70833.0
78-79	72976.0
80-81	71811.0
82-83	69158.0
84-85	68604.0
86-87	71233.0
88-89	73994.0
90-91	74278.0
92-93	74821.0
94-95	89228.0
96-97	294934.0
98-99	648095.0
100-101	6290157.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.99892746311775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.14864411759183	74.88390490668897
2	7.4331268030587	12.487493583085682
3	1.8949529520426718	4.775220466939597
4	0.7028805783135024	2.361648588519606
5	0.3061374163431533	1.2857607314577404
6	0.1742384675275183	0.8781506637077284
7	0.096730374885069	0.5687673420415769
8	0.05961205364234847	0.4005878855864891
9	0.04006564158323813	0.30289238290023246
>10	0.1403851678339522	1.7810364558186829
>50	0.002538509905079262	0.13728388137941697
>100	6.2301228683358E-4	0.07682734155558185
>500	2.1634996087432504E-5	0.01650123352047876
>1k	4.326998999971697E-5	0.043924536798327495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.67411663222502E-5	0.0	0.0	0.0
3	0.0	9.67411663222502E-5	0.0	0.0	0.0
4	0.0	1.3973724024325028E-4	0.0	2.1498036960500046E-5	0.0
5	0.0	1.504862587235003E-4	0.0	3.2247055440750066E-5	0.0
6	0.0	1.504862587235003E-4	0.0	5.374509240125011E-5	0.0
7	0.0	1.504862587235003E-4	0.0	6.449411088150013E-5	0.0
8	0.0	1.504862587235003E-4	0.0	7.524312936175015E-5	0.0
9	0.0	2.6872546200625057E-4	0.0	1.0749018480250022E-4	0.0
10-11	0.0	2.6872546200625057E-4	0.0	1.6660978644387534E-4	0.0
12-13	0.0	2.6872546200625057E-4	0.0	1.7735880492412536E-4	0.0
14-15	5.3745092401250115E-6	3.009725174470006E-4	0.0	1.934823326445004E-4	1.0749018480250023E-5
16-17	1.0749018480250023E-5	3.063470266871256E-4	0.0	2.1498036960500045E-4	2.1498036960500046E-5
18-19	1.0749018480250023E-5	3.385940821278757E-4	0.0	2.4722742504575053E-4	2.1498036960500046E-5
20-21	1.0749018480250023E-5	3.654666283285008E-4	0.0	2.4722742504575053E-4	2.1498036960500046E-5
22-23	2.1498036960500046E-5	3.923391745291258E-4	0.0	2.633509527661255E-4	2.1498036960500046E-5
24-25	3.224705544075007E-5	4.51458776170501E-4	0.0	2.7947448048650056E-4	3.2247055440750066E-5
26-27	4.299607392100009E-5	4.944548500915011E-4	0.0	3.0097251744700066E-4	3.2247055440750066E-5
28-29	4.299607392100009E-5	4.998293593316261E-4	0.0	5.320764147723761E-4	3.2247055440750066E-5
30-31	6.986862012162514E-5	5.750724886933762E-4	0.0	0.0014994880779948781	3.2247055440750066E-5
32-33	7.524312936175015E-5	5.804469979335012E-4	0.0	0.003402064348999132	4.299607392100009E-5
34-35	8.599214784200018E-5	6.718136550156264E-4	0.0	0.0062183071908246375	4.299607392100009E-5
36-37	9.67411663222502E-5	7.846783490582516E-4	0.0	0.010856508665052523	4.299607392100009E-5
38-39	9.67411663222502E-5	8.061763860187517E-4	0.0	0.023169509334178925	4.299607392100009E-5
40-41	9.67411663222502E-5	8.599214784200018E-4	0.0	0.03647679421272845	4.299607392100009E-5
42-43	9.67411663222502E-5	8.760450061403768E-4	0.0	0.04468904433163947	4.299607392100009E-5
44-45	9.67411663222502E-5	9.727861724626271E-4	0.0	0.05435241194538423	4.83705831611251E-5
46-47	9.67411663222502E-5	0.0010480293018243771	0.0	0.06494019514843051	5.374509240125011E-5
48-49	9.67411663222502E-5	0.0010587783203046272	0.0	0.07557634893463791	5.374509240125011E-5
50-51	9.67411663222502E-5	0.0011662685051071274	0.0	0.08612113606376318	5.374509240125011E-5
52-53	9.67411663222502E-5	0.0011716430143472523	0.0	0.11700844066676161	5.911960164137512E-5
54-55	9.67411663222502E-5	0.0012038900697880024	0.0	0.1558607679636253	6.449411088150013E-5
56-57	1.0749018480250022E-4	0.0012361371252287525	0.0	0.20913290355174444	6.449411088150013E-5
58-59	1.0749018480250022E-4	0.0012468861437090026	0.0	0.2439650979369946	6.449411088150013E-5
60-61	1.0749018480250022E-4	0.0012898822176300026	0.0	0.2582290454602864	6.449411088150013E-5
62-63	1.0749018480250022E-4	0.0013113802545905028	0.0	0.2702142010657652	6.449411088150013E-5
64-65	1.0749018480250022E-4	0.0014941135687547532	0.0	0.2770505768192042	6.449411088150013E-5
66-67	1.0749018480250022E-4	0.0015317351334356282	0.0	0.2835429839812752	6.986862012162514E-5
68-69	1.1823920328275024E-4	0.0015908547350770032	0.0	0.28985265782918196	7.524312936175015E-5
70-71	1.1823920328275024E-4	0.0016123527720375034	0.0	0.29493156906110013	7.524312936175015E-5
72-73	1.1823920328275024E-4	0.0016231017905177533	0.0	0.29876359414930925	8.599214784200018E-5
74-75	1.1823920328275024E-4	0.0016660978644387535	0.0	0.30163358208353597	8.599214784200018E-5
76-77	1.1823920328275024E-4	0.0017682135400011287	0.0	0.3046164347118054	8.599214784200018E-5
78-79	1.1823920328275024E-4	0.0018112096139221287	0.0	0.30658350509369114	8.599214784200018E-5
80-81	1.2898822176300026E-4	0.0018219586324023788	0.0	0.3072284462025061	8.599214784200018E-5
82-83	1.2898822176300026E-4	0.0018327076508826289	0.0	0.3076207853770353	9.67411663222502E-5
84-85	1.2898822176300026E-4	0.0018595801970832538	0.0	0.30780889320043964	9.67411663222502E-5
86-87	1.2898822176300026E-4	0.0018810782340437538	0.0	0.3079056343667619	9.67411663222502E-5
88	1.2898822176300026E-4	0.001945572344925254	0.0	0.30791638338524213	9.67411663222502E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10975	0.0	47.063457	1
GTATCAA	20430	0.0	39.147778	1
TCAACGC	23945	0.0	32.698856	4
ATCAACG	23935	0.0	32.658474	3
CAACGCA	24570	0.0	31.831982	5
TATCAAC	25300	0.0	31.186174	2
AACGCAG	25410	0.0	30.913765	6
ACGCAGA	29490	0.0	26.5634	7
CGCAGAG	30275	0.0	25.903118	8
GTGGTAT	5510	0.0	22.144526	1
GCAGAGT	35475	0.0	22.08188	9
GTACATG	24885	0.0	20.929594	1
TACATGG	24875	0.0	20.522022	2
GAGTACT	21450	0.0	19.70715	12-13
TGGTATC	5760	0.0	19.686333	2
ACATGGG	25115	0.0	19.673563	3
AGAGTAC	31865	0.0	18.873995	10-11
CAGAGTA	34380	0.0	18.759844	10-11
CATGGGG	19470	0.0	17.582722	4
GTACTTT	24115	0.0	17.57439	14-15
>>END_MODULE