FastQCFastQC Report
Fri 27 May 2016
SRR2138751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867008
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94950.049.4383471
GTATCAA180100.038.9171941
TCAACGC214550.032.046634
ATCAACG215900.031.8063893
CAACGCA221550.030.975845
TATCAAC224400.030.927562
AACGCAG229150.030.0036776
ACGCAGA261650.026.2438167
CGCAGAG268000.025.5738328
GCAGAGT312600.021.952639
GTGGTAT47650.021.4002861
GTACATG219700.020.7983361
GAGTACT187550.020.26769312-13
CTTATAC65100.020.1582581
TACATGG221700.020.0090792
TGGTATC46550.019.872522
CAGAGTA305300.018.89804310-11
ACATGGG224850.018.88683
AGAGTAC283200.018.70163710-11
GTACTTT211050.017.94993814-15
Produced by FastQC (version 0.11.5)