##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138751_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7867008
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.249039278973655	33.0	33.0	34.0	31.0	34.0
2	32.02729411232326	33.0	32.0	33.0	30.0	34.0
3	32.06045665645694	33.0	33.0	33.0	29.0	34.0
4	32.47807514623094	33.0	33.0	34.0	31.0	34.0
5	32.80214854236833	33.0	33.0	34.0	31.0	34.0
6	36.802147525463305	38.0	37.0	38.0	35.0	38.0
7	37.0946005139438	38.0	38.0	38.0	36.0	38.0
8	37.13454200122842	38.0	38.0	38.0	36.0	38.0
9	37.222984773880995	38.0	38.0	38.0	36.0	38.0
10-11	37.268684485893495	38.0	38.0	38.0	36.5	38.0
12-13	37.304188136074096	38.0	38.0	38.0	37.0	38.0
14-15	37.33121626417566	38.0	38.0	38.0	37.0	38.0
16-17	37.32689619738534	38.0	38.0	38.0	37.0	38.0
18-19	37.19953774039635	38.0	38.0	38.0	36.5	38.0
20-21	37.31603999116959	38.0	38.0	38.0	37.0	38.0
22-23	37.33788558828765	38.0	38.0	38.0	37.0	38.0
24-25	37.34359419069058	38.0	38.0	38.0	37.0	38.0
26-27	37.31412909490329	38.0	38.0	38.0	37.0	38.0
28-29	37.29102759741495	38.0	38.0	38.0	37.0	38.0
30-31	37.294145960416486	38.0	38.0	38.0	37.0	38.0
32-33	37.27870724957166	38.0	38.0	38.0	37.0	38.0
34-35	37.26563957296871	38.0	38.0	38.0	37.0	38.0
36-37	37.24176517614471	38.0	38.0	38.0	37.0	38.0
38-39	37.20383198643904	38.0	38.0	38.0	37.0	38.0
40-41	37.20544352998017	38.0	38.0	38.0	37.0	38.0
42-43	37.17871007041635	38.0	38.0	38.0	36.5	38.0
44-45	37.14861768672412	38.0	38.0	38.0	36.0	38.0
46-47	37.112178164725464	38.0	38.0	38.0	36.0	38.0
48-49	37.0803609510297	38.0	38.0	38.0	36.0	38.0
50-51	37.05528803911244	38.0	38.0	38.0	36.0	38.0
52-53	37.03525406602858	38.0	38.0	38.0	36.0	38.0
54-55	37.0182642891516	38.0	38.0	38.0	36.0	38.0
56-57	37.004905213425374	38.0	38.0	38.0	36.0	38.0
58-59	36.99424202241653	38.0	38.0	38.0	36.0	38.0
60-61	36.99024163878576	38.0	38.0	38.0	36.0	38.0
62-63	36.98393144895637	38.0	38.0	38.0	36.0	38.0
64-65	36.98658721260844	38.0	38.0	38.0	36.0	38.0
66-67	36.97586599326095	38.0	38.0	38.0	36.0	38.0
68-69	36.97614268053428	38.0	38.0	38.0	36.0	38.0
70-71	36.96626793972782	38.0	38.0	38.0	36.0	38.0
72-73	36.935796862342734	38.0	38.0	38.0	36.0	38.0
74-75	36.95498268701227	38.0	38.0	38.0	36.0	38.0
76-77	36.941353211120614	38.0	38.0	38.0	36.0	38.0
78-79	36.92865690717407	38.0	38.0	38.0	36.0	38.0
80-81	36.903072373754796	38.0	38.0	38.0	35.5	38.0
82-83	36.89151636700714	38.0	38.0	38.0	35.0	38.0
84-85	36.87356867781117	38.0	38.0	38.0	35.0	38.0
86-87	36.83796381782563	38.0	38.0	38.0	35.0	38.0
88-89	36.81780562764003	38.0	38.0	38.0	35.0	38.0
90-91	36.82716012899637	38.0	38.0	38.0	35.0	38.0
92-93	36.794062981449414	38.0	38.0	38.0	35.0	38.0
94-95	36.73370886742008	38.0	38.0	38.0	35.0	38.0
96-97	36.750096861907494	38.0	38.0	38.0	35.0	38.0
98-99	36.779939636700135	38.0	38.0	38.0	35.0	38.0
100	35.194352502828416	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	17.0
19	36.0
20	95.0
21	209.0
22	744.0
23	1966.0
24	4511.0
25	8892.0
26	15589.0
27	24292.0
28	36923.0
29	52996.0
30	71992.0
31	93984.0
32	122357.0
33	161664.0
34	229331.0
35	379751.0
36	919389.0
37	5742261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.534087673484	19.512081340199476	13.788939835830853	25.164891150485673
2	14.756957155757307	20.063472669660435	42.227947397536646	22.951622777045603
3	18.297896735328095	25.396961589463235	29.940111920567514	26.365029754641157
4	12.662781072550072	17.58710554253917	38.73883184051675	31.011281544394002
5	13.764710608214322	36.87899807311975	35.310938572969626	14.045352745696299
6	30.30028657738638	37.393296161108736	18.602145548771226	13.704271712733659
7	26.731916377865637	32.34495249019704	22.845775674818178	18.077355457119147
8	24.362896287889882	36.59279360082003	21.028833325198093	18.015476786091995
9	25.550539671499	16.785720822960904	21.15187883373196	36.51186067180814
10-11	24.39832907250126	26.484624141732155	28.671236892094175	20.445809893672408
12-13	25.20607105052334	23.98788230467557	28.755730816323744	22.050315828477345
14-15	22.41643486525435	25.225711633407727	26.430610777389674	25.927242723948257
16-17	22.043202447487023	28.370582564553132	27.123475659361223	22.462739328598623
18-19	22.03253765599323	27.37857899725029	28.837310194676295	21.75157315208018
20-21	22.76721918459814	26.612364422350165	28.825334369574573	21.795082023477118
22-23	22.505867608348474	26.581552923265804	28.67177279634012	22.2408066720456
24-25	22.275213175811054	26.697854816695244	28.8053496856267	22.221582321866997
26-27	22.203658534181947	26.829411722447688	28.75103951884955	22.215890224520813
28-29	22.093518623271923	26.798556747539752	28.80246637770838	22.305458251479944
30-31	22.343638131046315	26.689368582467267	28.744239261041884	22.222754025444537
32-33	22.149033712748075	26.81421939440031	28.747603632608605	22.289143260243012
34-35	22.32326693651783	26.900590363861944	28.7454630532736	22.030679646346623
36-37	22.30754871045463	26.79238049872164	28.601339670968564	22.29873111985517
38-39	22.16870605522156	26.87883414576517	28.748756151406933	22.203703647606343
40-41	22.447868900484742	26.706477264404633	28.63325229488317	22.212401540227454
42-43	22.37244507509535	26.80600522894427	28.470883197212345	22.350666498748044
44-45	22.34314483319505	26.969030291885197	28.3643689336063	22.32345594131345
46-47	22.487869283307973	26.860292407363453	28.08079982154212	22.571038487786456
48-49	22.44265770722586	26.945606259303208	28.043699679664957	22.56803635380598
50-51	22.351733344942172	27.15682575614664	27.980737655798965	22.510703243112225
52-53	22.517028165096047	27.103363145035996	27.937884759901298	22.441723929966656
54-55	22.502039581465148	26.992400929045445	27.90577116607542	22.599788323413986
56-57	22.530501179881032	27.155014775605864	27.788523506670625	22.52596053784248
58-59	22.427082678218348	27.233734200354263	27.961279853374034	22.377903268053355
60-61	22.548409812567428	27.319574666422486	27.79055065201798	22.341464868992105
62-63	22.450245350583277	27.45518381565161	27.753696266284507	22.340874567480608
64-65	22.47274449810836	27.36430240612957	27.74788879235816	22.41506430340391
66-67	22.483106834815025	27.406508686852998	27.58913132612132	22.52125315221066
68-69	22.42128049633683	27.313887018351952	27.733197914944935	22.531634570366286
70-71	22.495892042476118	27.07639655095268	27.741018060462004	22.686693346109198
72-73	22.599428590410326	26.99270201951165	27.687532905731526	22.720336484346497
74-75	22.474064256152857	27.160437269784367	27.669122547177295	22.69637592688548
76-77	22.62066223638107	27.059167025835556	27.570237616862634	22.74993312092074
78-79	22.53361695318008	27.117796340217005	27.548698725371345	22.799887981231574
80-81	22.59399618477074	27.159749979099384	27.485791963387456	22.760461872742418
82-83	22.6539389258322	27.12024528331936	27.595050479163714	22.630765311684726
84-85	22.74465512391202	27.15177718920629	27.4340114622102	22.669556224671496
86-87	22.575597981127935	27.1723800119126	27.555824634001063	22.696197372958398
88-89	22.63688714509722	27.14497534280052	27.504248553307654	22.713888958794612
90-91	22.672365183032912	27.162209364192712	27.49310024399485	22.67232520877953
92-93	22.733002522159875	27.137173654217317	27.4973748874043	22.63244893621851
94-95	22.597312103373255	27.225131061047836	27.52399581101081	22.6535610245681
96-97	22.692977557553	27.344678723475702	27.52577546110599	22.436568257865304
98-99	23.77811905265052	29.210740242413145	27.761719688247826	19.24942101668851
100	22.672954221608816	30.073555821298115	21.65172907760909	25.601760879483983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2122.0
1	1954.5
2	2258.0
3	2986.5
4	3766.0
5	5162.0
6	6527.5
7	8240.5
8	10354.5
9	11505.0
10	11239.0
11	10966.0
12	12087.5
13	14494.0
14	18959.0
15	24271.5
16	28462.5
17	30708.0
18	30200.0
19	27707.0
20	24754.0
21	22792.0
22	23236.0
23	26640.0
24	33174.5
25	42815.5
26	55639.5
27	70326.5
28	85066.0
29	100647.0
30	116473.5
31	132543.0
32	150241.5
33	167641.5
34	184331.0
35	201316.0
36	220195.0
37	236972.0
38	249370.0
39	259860.5
40	272145.5
41	284014.0
42	292666.5
43	306329.5
44	317692.5
45	324428.0
46	327800.5
47	326968.5
48	327160.5
49	325805.5
50	322942.5
51	317251.5
52	306941.0
53	293515.5
54	279051.5
55	261959.5
56	242653.5
57	221465.5
58	198345.5
59	174137.0
60	147485.0
61	120807.0
62	96191.0
63	74760.0
64	57252.0
65	43364.0
66	32738.5
67	24891.0
68	18753.5
69	14150.0
70	10577.5
71	7732.0
72	5731.0
73	4084.0
74	2785.0
75	1834.0
76	1179.5
77	730.0
78	463.5
79	283.5
80	174.0
81	107.5
82	68.5
83	46.5
84	32.5
85	24.0
86	17.5
87	13.0
88	9.0
89	6.5
90	5.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.005160793023217976
6	2.9236019589658484E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.3556564325344536E-6
14-15	1.2711312865068907E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	4.463100772416099E-5
34-35	1.469868244205955E-4
36-37	1.3466847507190334E-4
38-39	1.546852519278133E-4
40-41	1.166641216604571E-4
42-43	1.6948279717015876E-4
44-45	1.837626532071899E-4
46-47	3.969257308295787E-5
48-49	3.4008558820636524E-4
50-51	3.4262084388320016E-4
52-53	4.66675765716862E-4
54-55	0.0019951104133596404
56-57	0.003168746448020418
58-59	0.001997268924796882
60-61	0.00329479376611088
62-63	0.0020149430139714186
64-65	0.0026252963365157207
66-67	7.209487800154712E-4
68-69	6.916895944955342E-4
70-71	3.710516826975545E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	7.91385484106724E-6
90-91	0.0
92-93	8.077890248936344E-6
94-95	2.4495096857287238E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	66.0
20-21	289.0
22-23	685.0
24-25	811.0
26-27	1874.0
28-29	4392.0
30-31	12955.0
32-33	16958.0
34-35	23448.0
36-37	37126.0
38-39	43331.0
40-41	43090.0
42-43	49207.0
44-45	59225.0
46-47	61042.0
48-49	56733.0
50-51	51073.0
52-53	48870.0
54-55	52392.0
56-57	54648.0
58-59	56365.0
60-61	57026.0
62-63	55384.0
64-65	59239.0
66-67	64393.0
68-69	66300.0
70-71	71545.0
72-73	59115.0
74-75	58732.0
76-77	61067.0
78-79	62971.0
80-81	62921.0
82-83	59473.0
84-85	59480.0
86-87	60995.0
88-89	63489.0
90-91	64511.0
92-93	64422.0
94-95	76391.0
96-97	247424.0
98-99	544276.0
100-101	5273274.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.08261757822693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74699722116848	76.35909443362874
2	7.141158481876363	12.151769123579962
3	1.738472288989905	4.437413188034186
4	0.6416705307348707	2.1838003351093165
5	0.28784824591557995	1.2245441113899354
6	0.15316912508940014	0.7819218056863821
7	0.08570335053807704	0.5104305779302757
8	0.051336770785800394	0.34942934691754773
9	0.034711848875098046	0.2658037466945856
>10	0.11623793574262267	1.501531514535216
>50	0.002184118975165233	0.12194335757351
>100	4.3818165451740674E-4	0.05681315217645402
>500	4.7933103920048046E-5	0.03291167069404221
>1k	2.3966550226740317E-5	0.022593636049931146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2711312865068905E-5	0.0
3	0.0	0.0	0.0	1.2711312865068905E-5	0.0
4	0.0	1.1440181578562015E-4	0.0	2.542262573013781E-5	0.0
5	0.0	1.1440181578562015E-4	0.0	2.542262573013781E-5	0.0
6	0.0	1.1440181578562015E-4	0.0	2.542262573013781E-5	2.542262573013781E-5
7	0.0	1.1440181578562015E-4	0.0	2.542262573013781E-5	2.542262573013781E-5
8	0.0	1.2711312865068905E-4	0.0	2.542262573013781E-5	2.542262573013781E-5
9	0.0	3.9405069881713606E-4	0.0	2.542262573013781E-5	5.084525146027562E-5
10-11	0.0	3.9405069881713606E-4	0.0	3.813393859520672E-5	6.991222075787898E-5
12-13	0.0	3.9405069881713606E-4	0.0	3.813393859520672E-5	8.897919005548234E-5
14-15	0.0	5.720090789281008E-4	0.0	5.084525146027562E-5	1.1440181578562015E-4
16-17	0.0	5.720090789281008E-4	0.0	5.084525146027562E-5	1.1440181578562015E-4
18-19	0.0	6.03787361090773E-4	0.0	5.084525146027562E-5	1.1440181578562015E-4
20-21	0.0	6.546326125510486E-4	0.0	5.084525146027562E-5	1.1440181578562015E-4
22-23	0.0	7.690344283366688E-4	0.0	6.355656432534452E-5	1.2075747221815459E-4
24-25	0.0	8.516579619596166E-4	0.0	7.626787719041344E-5	1.2711312865068905E-4
26-27	0.0	8.897919005548233E-4	0.0	1.2075747221815459E-4	1.2711312865068905E-4
28-29	0.0	9.025032134198922E-4	0.0	3.495611037893949E-4	1.334687850832235E-4
30-31	0.0	0.0010359719985031158	0.0	0.0011567294707212703	1.4618009794829242E-4
32-33	0.0	0.001067750280665788	0.0	0.003126982964806951	1.9066969297603357E-4
34-35	0.0	0.0012393530043442182	0.0	0.005573910691332715	2.033810058411025E-4
36-37	0.0	0.0014490896666178553	0.0	0.009533484648801679	2.033810058411025E-4
38-39	0.0	0.0015317132002408032	0.0	0.019244927677714324	2.160923187061714E-4
40-41	0.0	0.0017033159239192334	0.0	0.029623714632043087	2.288036315712403E-4
42-43	0.0	0.00174144986251444	0.0	0.03620181903971624	2.6058191373391255E-4
44-45	0.0	0.001982964806950749	0.0	0.04394936423097574	2.7329322659898147E-4
46-47	0.0	0.0021863458127918517	0.0	0.0527773710157661	2.9236019589658484E-4
48-49	0.0	0.0021863458127918517	0.0	0.061681645677746866	2.9236019589658484E-4
50-51	0.0	0.0025231956037161775	0.0	0.07014102438945022	2.9236019589658484E-4
52-53	0.0	0.002542262573013781	0.0	0.08965924529376353	3.1142716519418816E-4
54-55	0.0	0.0025549738858788498	0.0	0.11225995956785603	3.3049413449179153E-4
56-57	0.0	0.0026185304502041947	0.0	0.14122268593091553	3.3049413449179153E-4
58-59	0.0	0.0026312417630692635	0.0	0.1641475386830673	3.36849790924326E-4
60-61	0.0	0.002694798327394608	0.0	0.17306452465791317	3.4320544735686045E-4
62-63	0.0	0.00278377751745009	0.0	0.1839962537218724	3.5591676022192937E-4
64-65	0.0	0.003050715087616537	0.0	0.18965278794682808	3.5591676022192937E-4
66-67	0.0	0.0030952046826442786	0.0	0.19486442622150632	3.5591676022192937E-4
68-69	0.0	0.0031905395291322953	0.0	0.20045740388213662	3.5591676022192937E-4
70-71	0.0	0.003241384780592571	0.0	0.2054148158995135	3.5591676022192937E-4
72-73	0.0	0.003285874375620312	0.0	0.20938710116984754	3.686280730869983E-4
74-75	0.0	0.0033812092221083287	0.0	0.21241239363173395	3.7498372951953274E-4
76-77	0.0	0.003495611037893949	0.0	0.21538684084216006	3.8133938595206714E-4
78-79	0.0	0.0035464562893542245	0.0	0.21759860928068206	3.8133938595206714E-4
80-81	0.0	0.003552811945786759	0.0	0.2183040871446934	3.8133938595206714E-4
82-83	0.0	0.0035591676022192933	0.0	0.2186600039049153	3.8133938595206714E-4
84-85	0.0	0.0035845902279494314	0.0	0.21881889531572868	3.8133938595206714E-4
86-87	0.0	0.003648146792274776	0.0	0.2189015188493516	3.8133938595206714E-4
88	0.0	0.0038261051723857404	0.0	0.2189142301622167	3.8133938595206714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9495	0.0	49.438347	1
GTATCAA	18010	0.0	38.917194	1
TCAACGC	21455	0.0	32.04663	4
ATCAACG	21590	0.0	31.806389	3
CAACGCA	22155	0.0	30.97584	5
TATCAAC	22440	0.0	30.92756	2
AACGCAG	22915	0.0	30.003677	6
ACGCAGA	26165	0.0	26.243816	7
CGCAGAG	26800	0.0	25.573832	8
GCAGAGT	31260	0.0	21.95263	9
GTGGTAT	4765	0.0	21.400286	1
GTACATG	21970	0.0	20.798336	1
GAGTACT	18755	0.0	20.267693	12-13
CTTATAC	6510	0.0	20.158258	1
TACATGG	22170	0.0	20.009079	2
TGGTATC	4655	0.0	19.87252	2
CAGAGTA	30530	0.0	18.898043	10-11
ACATGGG	22485	0.0	18.8868	3
AGAGTAC	28320	0.0	18.701637	10-11
GTACTTT	21105	0.0	17.949938	14-15
>>END_MODULE