FastQCFastQC Report
Fri 27 May 2016
SRR2138750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9765380
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132450.053.1648641
GTATCAA249400.039.801491
TCAACGC304750.031.9641384
ATCAACG309500.031.4875073
TATCAAC314800.031.2313372
CAACGCA313500.031.0582435
AACGCAG320600.030.4092186
GTGGTAT57700.027.9502871
TGGTATC56000.027.5667442
ACGCAGA363800.026.7626727
CGCAGAG395500.024.6394048
GCAGAGT435650.022.3785069
GTACATG311850.021.7645361
TACATGG309900.021.3449062
ACATGGG312850.020.5647353
GAGTACT262000.020.41567412-13
CAGAGTA417700.019.95942110-11
AGAGTAC394950.019.04024910-11
CATGGGG243800.018.6775844
GTACTTT292800.018.1725414-15
Produced by FastQC (version 0.11.5)