FastQCFastQC Report
Fri 27 May 2016
SRR2138749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998008
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127550.041.744071
GTATCAA219650.035.392121
TCAACGC242600.031.435364
CAACGCA243300.031.2021185
ATCAACG244800.031.099633
AACGCAG247150.030.7307326
TATCAAC263950.029.0736542
ACGCAGA280400.027.1331257
CGCAGAG285100.026.6553618
GCAGAGT340100.022.3191959
GTACATG230250.020.314251
GAGTACT212450.020.16434312-13
TACATGG228150.019.7588482
AGAGTAC303450.019.31209810-11
CAGAGTA332350.019.27258910-11
ACATGGG228550.018.9261743
GTACTTT242500.017.57687214-15
CATGGGG174500.017.197584
AGTACTT237500.016.52907612-13
ACTTTTT290850.014.84261716-17
Produced by FastQC (version 0.11.5)