##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8998008
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19006962429907	33.0	32.0	34.0	31.0	34.0
2	32.00907434178765	33.0	32.0	33.0	30.0	34.0
3	32.02786661225463	33.0	31.0	33.0	29.0	34.0
4	32.47609982120487	33.0	33.0	34.0	31.0	34.0
5	32.78654008753938	33.0	33.0	34.0	31.0	34.0
6	36.77398141899852	38.0	37.0	38.0	35.0	38.0
7	37.05372377975214	38.0	38.0	38.0	36.0	38.0
8	37.08479532358717	38.0	38.0	38.0	36.0	38.0
9	37.174936052512955	38.0	38.0	38.0	36.0	38.0
10-11	37.22909826263769	38.0	38.0	38.0	36.0	38.0
12-13	37.26803710332331	38.0	38.0	38.0	36.5	38.0
14-15	37.29521745257395	38.0	38.0	38.0	37.0	38.0
16-17	37.291362210391455	38.0	38.0	38.0	37.0	38.0
18-19	37.16277813933928	38.0	38.0	38.0	36.0	38.0
20-21	37.27840559361856	38.0	38.0	38.0	37.0	38.0
22-23	37.29983836182225	38.0	38.0	38.0	37.0	38.0
24-25	37.30673089007509	38.0	38.0	38.0	37.0	38.0
26-27	37.272938645473324	38.0	38.0	38.0	37.0	38.0
28-29	37.2503840482769	38.0	38.0	38.0	37.0	38.0
30-31	37.25298686479498	38.0	38.0	38.0	37.0	38.0
32-33	37.238601678559476	38.0	38.0	38.0	37.0	38.0
34-35	37.22483826390717	38.0	38.0	38.0	37.0	38.0
36-37	37.202502996692104	38.0	38.0	38.0	36.5	38.0
38-39	37.16943013304348	38.0	38.0	38.0	36.0	38.0
40-41	37.16094635647257	38.0	38.0	38.0	36.0	38.0
42-43	37.13407497068086	38.0	38.0	38.0	36.0	38.0
44-45	37.10346399013924	38.0	38.0	38.0	36.0	38.0
46-47	37.069640174744606	38.0	38.0	38.0	36.0	38.0
48-49	37.03609151275124	38.0	38.0	38.0	36.0	38.0
50-51	37.01131109004872	38.0	38.0	38.0	36.0	38.0
52-53	36.989926301301026	38.0	38.0	38.0	36.0	38.0
54-55	36.974098996611346	38.0	38.0	38.0	36.0	38.0
56-57	36.95945947431645	38.0	38.0	38.0	36.0	38.0
58-59	36.94710777721667	38.0	38.0	38.0	36.0	38.0
60-61	36.94061529969882	38.0	38.0	38.0	36.0	38.0
62-63	36.93337265446637	38.0	38.0	38.0	36.0	38.0
64-65	36.93622523766096	38.0	38.0	38.0	36.0	38.0
66-67	36.92613962904764	38.0	38.0	38.0	36.0	38.0
68-69	36.9265272031916	38.0	38.0	38.0	36.0	38.0
70-71	36.914491963142794	38.0	38.0	38.0	35.5	38.0
72-73	36.884975663929225	38.0	38.0	38.0	35.0	38.0
74-75	36.89972136662976	38.0	38.0	38.0	35.0	38.0
76-77	36.886999292384786	38.0	38.0	38.0	35.0	38.0
78-79	36.87348132087219	38.0	38.0	38.0	35.0	38.0
80-81	36.84663887929999	38.0	38.0	38.0	35.0	38.0
82-83	36.832240547958705	38.0	38.0	38.0	35.0	38.0
84-85	36.8096081787898	38.0	38.0	38.0	35.0	38.0
86-87	36.76867269530432	38.0	38.0	38.0	35.0	38.0
88-89	36.74883000876727	38.0	38.0	38.0	35.0	38.0
90-91	36.75428479957205	38.0	38.0	38.0	35.0	38.0
92-93	36.71852795272027	38.0	38.0	38.0	35.0	38.0
94-95	36.65945704424152	38.0	38.0	38.0	34.0	38.0
96-97	36.67539003256816	38.0	38.0	38.0	34.0	38.0
98-99	36.70078817284238	38.0	38.0	38.0	34.0	38.0
100	35.085780429064116	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	21.0
19	41.0
20	95.0
21	264.0
22	889.0
23	2500.0
24	5596.0
25	10855.0
26	18843.0
27	30145.0
28	44878.0
29	64824.0
30	87300.0
31	114729.0
32	148871.0
33	197120.0
34	278586.0
35	458192.0
36	1089284.0
37	6444974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55749805957052	19.596348436231665	13.80655585102836	25.03959765316946
2	14.448097845656505	20.23197801113313	42.697894911851606	22.622029231358763
3	18.136002990884204	25.844620275954412	30.036548089310433	25.98282864385095
4	12.358468674399933	17.71692134525775	39.582961028707686	30.34164895163463
5	13.505603856336956	37.12765713881795	35.62566004049545	13.741078964349645
6	29.789468349681076	38.007022018937135	18.946870531636982	13.25663909974481
7	26.281533930962038	32.558758018165356	23.4650988230059	17.694609227866707
8	23.949189642863175	37.033507860850975	21.28758943090515	17.729713065380693
9	25.420637545554527	16.922667772689245	21.399214137173473	36.257480544582755
10-11	24.07321709427242	26.677143429968055	29.109570696091847	20.14006877966768
12-13	24.988326582301692	24.245406552506207	29.220191247895716	21.546075617296385
14-15	22.145577467631302	25.498197850823956	26.864167079674818	25.492057601869927
16-17	21.72037410946956	28.579642294161108	27.504348740298962	22.195634856070363
18-19	21.703331448471705	27.671419051861257	29.219728410999412	21.405521088667626
20-21	22.47965707772321	26.9953434890868	29.138002010286158	21.386997422903836
22-23	22.184071899874752	26.895447449411396	29.035019017148084	21.885461633565768
24-25	21.9797848360092	26.928980142825726	29.16843063166982	21.92280438949526
26-27	21.96490262618851	27.084488839602113	29.038490935216604	21.91211759899277
28-29	21.914090145007602	27.125405177464057	29.065525208299135	21.8949794692292
30-31	22.10509323388208	27.013116385334694	29.004532387699854	21.877257993083365
32-33	21.91555073959232	27.13819868731685	29.00683428581875	21.939416287272085
34-35	22.057157530119373	27.165240133488343	28.975083016959907	21.802519319432374
36-37	22.053948634174077	27.118506518964118	28.918446524511026	21.90909832235078
38-39	21.90881762174175	27.235391912551975	28.98948079986066	21.866309665845613
40-41	22.168246495748246	27.075496744915917	28.861140445745065	21.89511631359077
42-43	21.983942080810937	27.188487463013832	28.763471786381267	22.06409866979396
44-45	22.1117815744121	27.252252857515252	28.63597277250523	21.999992795567422
46-47	22.163306861630325	27.261808221298246	28.377854099236988	22.197030817834438
48-49	22.099805761466545	27.336690253326573	28.391844579483084	22.171659405723798
50-51	22.120182743830544	27.45905719453548	28.28843807136277	22.132321990271205
52-53	22.24779338769891	27.483840602723998	28.137896265497993	22.130469744079097
54-55	22.22044135047046	27.367579162097805	28.213909722493607	22.198069764938133
56-57	22.19095797727622	27.466100061902964	28.18370910533014	22.15923285549067
58-59	22.206165313613955	27.471173514741448	28.173942399700298	22.1487187719443
60-61	22.250939054081922	27.43380179463334	28.218915404228856	22.096343747055887
62-63	22.211082226539375	27.506936632738572	28.178069373377674	22.10391176734438
64-65	22.206095684432427	27.442340352782836	28.18789018300955	22.163673779775184
66-67	22.27087173638506	27.462602817065267	28.03721743292835	22.229308013621317
68-69	22.21928885763462	27.42645873195823	28.14019766310323	22.214054747303923
70-71	22.24728613784948	27.250439084421362	28.14047997885185	22.361794798877305
72-73	22.300576926230907	27.28674987289456	28.047162110846035	22.3655110900285
74-75	22.15091369147727	27.46480506705611	28.05612429739932	22.328156944067302
76-77	22.320804886996843	27.390180547667875	27.971564654683938	22.317449910651348
78-79	22.254429659140623	27.39715241798973	27.91067800336895	22.437739919500697
80-81	22.28421499547576	27.45384523674409	27.86961035336944	22.392329414410707
82-83	22.302599276376828	27.45740704453077	27.97579759130896	22.264196087783443
84-85	22.439207826483837	27.383346893417738	27.871860509726883	22.30558477037155
86-87	22.266970989692265	27.440744641653236	27.958794660556208	22.33348970809829
88-89	22.33762253460542	27.43424886328885	27.88883922720445	22.339289374901284
90-91	22.360381080202828	27.508905035915802	27.83419110646495	22.296522777416417
92-93	22.40497181831913	27.460886569940712	27.874832917539166	22.259308694200993
94-95	22.26803780394642	27.567363955393105	27.867991430265338	22.29660681039514
96-97	22.364522624888224	27.68733539728472	27.925014203766192	22.02312777406087
98-99	23.4445001498605	29.533249911239963	28.16524950375241	18.857000435147132
100	22.359616473437384	30.524013815739497	22.048420834931786	25.067948875891332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2235.0
1	2051.0
2	2592.0
3	3655.0
4	4625.5
5	6276.0
6	7816.0
7	10032.5
8	12902.5
9	14137.0
10	13263.5
11	12539.0
12	13794.0
13	16709.5
14	21842.5
15	27621.0
16	31975.5
17	34399.5
18	33927.0
19	31495.5
20	29118.5
21	28088.0
22	29644.0
23	34275.0
24	42621.0
25	55167.5
26	70739.5
27	87469.0
28	105115.0
29	124894.5
30	143581.5
31	162023.0
32	181970.5
33	200301.0
34	219645.5
35	237417.5
36	255657.5
37	275337.0
38	290615.5
39	302715.5
40	316488.0
41	330611.5
42	341136.5
43	350831.5
44	358554.5
45	366409.0
46	372795.5
47	374529.0
48	373980.0
49	370777.0
50	364305.5
51	353196.0
52	337945.5
53	319941.0
54	300137.0
55	278519.5
56	256320.5
57	231868.0
58	206137.5
59	179881.5
60	150452.0
61	121838.5
62	95924.5
63	73331.5
64	55071.0
65	41159.0
66	30942.0
67	23296.0
68	17370.0
69	12915.5
70	9513.0
71	6641.0
72	4518.0
73	3044.5
74	2019.0
75	1265.0
76	770.0
77	456.0
78	287.0
79	196.5
80	127.0
81	77.0
82	50.5
83	36.5
84	25.0
85	15.5
86	11.5
87	7.5
88	5.0
89	4.5
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.005045561195322342
6	2.8895284378497996E-4
7	2.2227141829613845E-5
8	0.0
9	0.0
10-11	0.0
12-13	5.556785457403461E-6
14-15	1.6670356372210382E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.9014191635145526E-5
34-35	1.340287489432671E-4
36-37	1.792213459791915E-4
38-39	1.3501714717769158E-4
40-41	7.9168106065357E-5
42-43	1.5346933634345435E-4
44-45	1.257915217086148E-4
46-47	2.8789436994345927E-5
48-49	3.073385070968231E-4
50-51	4.7879434445784744E-4
52-53	3.5255139979064324E-4
54-55	0.0019687080651880034
56-57	0.0031718164647743
58-59	0.0018755012321983176
60-61	0.0034339921528752956
62-63	0.0020542659263043644
64-65	0.0027105718113380832
66-67	6.901563857397423E-4
68-69	7.761076080028242E-4
70-71	3.942732252119328E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	1.3442258643573941E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	166.0
20-21	587.0
22-23	1003.0
24-25	941.0
26-27	2014.0
28-29	4627.0
30-31	13706.0
32-33	16692.0
34-35	22481.0
36-37	36572.0
38-39	46124.0
40-41	44802.0
42-43	49476.0
44-45	59308.0
46-47	61948.0
48-49	60351.0
50-51	54988.0
52-53	51535.0
54-55	54324.0
56-57	57347.0
58-59	59061.0
60-61	59255.0
62-63	55162.0
64-65	56492.0
66-67	60513.0
68-69	62980.0
70-71	64052.0
72-73	59017.0
74-75	58254.0
76-77	60962.0
78-79	63804.0
80-81	64885.0
82-83	60746.0
84-85	59053.0
86-87	59734.0
88-89	64765.0
90-91	67466.0
92-93	67732.0
94-95	79994.0
96-97	278827.0
98-99	629786.0
100-101	6266476.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.41684772998282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.66217046803055	73.95918773341485
2	7.676723719213349	12.807361871015349
3	2.0273611674804393	5.073482334041881
4	0.751451961087248	2.507350152576478
5	0.34738199404451586	1.4488755450674593
6	0.18858553775569784	0.9438726652226386
7	0.10871973855777345	0.6348340513562132
8	0.061106272356291506	0.40778340931933016
9	0.04015802460199637	0.3014870241025472
>10	0.13324504439400825	1.6562130322159745
>50	0.0026337717124837725	0.14553579760597912
>100	3.9458582482447796E-4	0.05030054942724751
>500	2.257164722848531E-5	0.01743524341042813
>1k	4.514329358295909E-5	0.04628059122364644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.445428365922769E-5	0.0	0.0	0.0
3	0.0	5.556785457403461E-5	0.0	1.1113570914806922E-5	0.0
4	0.0	1.1113570914806922E-4	0.0	1.1113570914806922E-5	1.1113570914806922E-5
5	0.0	1.1113570914806922E-4	0.0	1.1113570914806922E-5	1.1113570914806922E-5
6	0.0	1.1113570914806922E-4	0.0	1.1113570914806922E-5	1.1113570914806922E-5
7	0.0	1.3336285097768306E-4	0.0	1.1113570914806922E-5	1.1113570914806922E-5
8	0.0	1.3336285097768306E-4	0.0	1.1113570914806922E-5	1.1113570914806922E-5
9	0.0	3.111799856145938E-4	0.0	2.2227141829613845E-5	1.1113570914806922E-5
10-11	0.0	3.111799856145938E-4	0.0	8.890856731845538E-5	1.1113570914806922E-5
12-13	0.0	3.222935565294007E-4	0.0	8.890856731845538E-5	1.1113570914806922E-5
14-15	0.0	5.612353311977496E-4	0.0	1.0002213823326229E-4	1.6670356372210382E-5
16-17	0.0	6.001328293995737E-4	0.0	1.0557892369066576E-4	4.445428365922769E-5
18-19	0.0	6.279167566865911E-4	0.0	1.1113570914806922E-4	4.445428365922769E-5
20-21	0.0	6.557006839736084E-4	0.0	1.1113570914806922E-4	4.445428365922769E-5
22-23	0.0	7.168253240050465E-4	0.0	1.3336285097768306E-4	5.001106911663115E-5
24-25	1.1113570914806922E-5	8.44631389525326E-4	0.0	1.6670356372210383E-4	5.556785457403461E-5
26-27	1.1113570914806922E-5	9.27983171386378E-4	0.0	2.0560106192392803E-4	5.556785457403461E-5
28-29	1.1113570914806922E-5	9.335399568437814E-4	0.0	3.50077483816418E-4	5.556785457403461E-5
30-31	1.1113570914806922E-5	0.0010335620950770437	0.0	9.27983171386378E-4	6.112464003143807E-5
32-33	1.1113570914806922E-5	0.0010446756659918505	0.0	0.002122692044728122	7.779499640364845E-5
34-35	1.1113570914806922E-5	0.0011280274478529025	0.0	0.003495218052706777	7.779499640364845E-5
36-37	1.1113570914806922E-5	0.0012224928006287614	0.0	0.005762386519327389	7.779499640364845E-5
38-39	1.1113570914806922E-5	0.0012725038697453926	0.0	0.009257604572034166	7.779499640364845E-5
40-41	1.1113570914806922E-5	0.001433650648010093	0.0	0.013364069025055323	7.779499640364845E-5
42-43	1.1113570914806922E-5	0.0014503210043823032	0.0	0.017214921347035922	7.779499640364845E-5
44-45	1.1113570914806922E-5	0.0015170024298711447	0.0	0.02149920293469399	7.779499640364845E-5
46-47	1.1113570914806922E-5	0.001567013498987776	0.0	0.02620024343165732	7.779499640364845E-5
48-49	1.1113570914806922E-5	0.001567013498987776	0.0	0.03095129499773728	8.890856731845538E-5
50-51	1.1113570914806922E-5	0.001833739200943142	0.0	0.03608576476037807	8.890856731845538E-5
52-53	1.1113570914806922E-5	0.001844852771857949	0.0	0.04079791882825621	9.446535277585883E-5
54-55	1.1113570914806922E-5	0.0018893070555171766	0.0	0.04525446076509378	1.0557892369066576E-4
56-57	1.1113570914806922E-5	0.0019337613391764044	0.0	0.04985547912382385	1.1669249460547268E-4
58-59	1.1113570914806922E-5	0.001972658837378229	0.0	0.0542731235624596	1.3336285097768306E-4
60-61	1.1113570914806922E-5	0.0019948859792078425	0.0	0.05881301728115823	1.3336285097768306E-4
62-63	1.1113570914806922E-5	0.002000442764665246	0.0	0.06308062851244409	1.3336285097768306E-4
64-65	1.1113570914806922E-5	0.002267168466620612	0.0	0.06689814012168027	1.3336285097768306E-4
66-67	1.1113570914806922E-5	0.002267168466620612	0.0	0.07115463778205132	1.3336285097768306E-4
68-69	1.1113570914806922E-5	0.002306065964822436	0.0	0.07543891936970938	1.3336285097768306E-4
70-71	1.1113570914806922E-5	0.0023560770339390675	0.0	0.07933978276080661	1.3336285097768306E-4
72-73	1.1113570914806922E-5	0.0023838609612260846	0.0	0.08257938868247283	1.3336285097768306E-4
74-75	1.1113570914806922E-5	0.0024227584594279086	0.0	0.08497992000007112	1.3336285097768306E-4
76-77	1.1113570914806922E-5	0.0025172238122037676	0.0	0.08743046238678605	1.3336285097768306E-4
78-79	1.1113570914806922E-5	0.002567234881320399	0.0	0.0891086115949219	1.3336285097768306E-4
80-81	1.1113570914806922E-5	0.002567234881320399	0.0	0.08961427907154562	1.3336285097768306E-4
82-83	1.1113570914806922E-5	0.0025727916667778023	0.0	0.09012550333362673	1.4447642189248998E-4
84-85	1.1113570914806922E-5	0.002589462023150013	0.0	0.09033110439555067	1.4447642189248998E-4
86-87	1.1113570914806922E-5	0.002722824874127696	0.0	0.0903922290355821	1.4447642189248998E-4
88	1.1113570914806922E-5	0.002845074154190572	0.0	0.0903977858210395	1.4447642189248998E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12755	0.0	41.74407	1
GTATCAA	21965	0.0	35.39212	1
TCAACGC	24260	0.0	31.43536	4
CAACGCA	24330	0.0	31.202118	5
ATCAACG	24480	0.0	31.09963	3
AACGCAG	24715	0.0	30.730732	6
TATCAAC	26395	0.0	29.073654	2
ACGCAGA	28040	0.0	27.133125	7
CGCAGAG	28510	0.0	26.655361	8
GCAGAGT	34010	0.0	22.319195	9
GTACATG	23025	0.0	20.31425	1
GAGTACT	21245	0.0	20.164343	12-13
TACATGG	22815	0.0	19.758848	2
AGAGTAC	30345	0.0	19.312098	10-11
CAGAGTA	33235	0.0	19.272589	10-11
ACATGGG	22855	0.0	18.926174	3
GTACTTT	24250	0.0	17.576872	14-15
CATGGGG	17450	0.0	17.19758	4
AGTACTT	23750	0.0	16.529076	12-13
ACTTTTT	29085	0.0	14.842617	16-17
>>END_MODULE