FastQCFastQC Report
Fri 27 May 2016
SRR2138748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6954901
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107900.049.7231141
GTATCAA197750.038.34841
TCAACGC236800.031.3890724
ATCAACG237500.031.2965563
CAACGCA241450.030.7489515
TATCAAC246100.030.3950482
AACGCAG248850.029.8676226
ACGCAGA284900.026.1032077
CGCAGAG289700.025.641038
GTGGTAT50350.025.2730371
TGGTATC50050.023.8784372
GCAGAGT328650.022.576029
GTACATG245900.020.437191
TACATGG241500.020.435722
GAGTACT190450.020.3481512-13
ACATGGG242850.019.7379463
CAGAGTA326700.019.42784110-11
AGAGTAC303600.018.76800710-11
GTACTTT208700.018.25378614-15
CATGGGG165800.017.5537284
Produced by FastQC (version 0.11.5)