##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6954901
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18762855143445	33.0	32.0	34.0	31.0	34.0
2	32.007662078870716	33.0	32.0	33.0	30.0	34.0
3	32.05200217803244	33.0	33.0	33.0	29.0	34.0
4	32.486064575182304	33.0	33.0	34.0	31.0	34.0
5	32.80282465559179	33.0	33.0	34.0	31.0	34.0
6	36.80748237825384	38.0	37.0	38.0	35.0	38.0
7	37.0805572933389	38.0	38.0	38.0	36.0	38.0
8	37.11968725363596	38.0	38.0	38.0	36.0	38.0
9	37.20558955476145	38.0	38.0	38.0	36.0	38.0
10-11	37.251748730859006	38.0	38.0	38.0	36.5	38.0
12-13	37.286664253021	38.0	38.0	38.0	37.0	38.0
14-15	37.314115398623215	38.0	38.0	38.0	37.0	38.0
16-17	37.31102175286176	38.0	38.0	38.0	37.0	38.0
18-19	37.18585311279053	38.0	38.0	38.0	36.5	38.0
20-21	37.301866542647346	38.0	38.0	38.0	37.0	38.0
22-23	37.32212672896104	38.0	38.0	38.0	37.0	38.0
24-25	37.32671996035724	38.0	38.0	38.0	37.0	38.0
26-27	37.29460127530889	38.0	38.0	38.0	37.0	38.0
28-29	37.271920790684185	38.0	38.0	38.0	37.0	38.0
30-31	37.2759636664521	38.0	38.0	38.0	37.0	38.0
32-33	37.25950217605748	38.0	38.0	38.0	37.0	38.0
34-35	37.24674417250989	38.0	38.0	38.0	37.0	38.0
36-37	37.223958945810054	38.0	38.0	38.0	37.0	38.0
38-39	37.1909217753607	38.0	38.0	38.0	36.5	38.0
40-41	37.18103426102752	38.0	38.0	38.0	36.0	38.0
42-43	37.15381529031433	38.0	38.0	38.0	36.0	38.0
44-45	37.121519864980144	38.0	38.0	38.0	36.0	38.0
46-47	37.08664672638798	38.0	38.0	38.0	36.0	38.0
48-49	37.05593002064131	38.0	38.0	38.0	36.0	38.0
50-51	37.035683482043666	38.0	38.0	38.0	36.0	38.0
52-53	37.01785113249515	38.0	38.0	38.0	36.0	38.0
54-55	37.00248246208817	38.0	38.0	38.0	36.0	38.0
56-57	36.99151274330251	38.0	38.0	38.0	36.0	38.0
58-59	36.97961287834006	38.0	38.0	38.0	36.0	38.0
60-61	36.97529629553124	38.0	38.0	38.0	36.0	38.0
62-63	36.97084722670586	38.0	38.0	38.0	36.0	38.0
64-65	36.97281042263563	38.0	38.0	38.0	36.0	38.0
66-67	36.96610748027054	38.0	38.0	38.0	36.0	38.0
68-69	36.96769070641223	38.0	38.0	38.0	36.0	38.0
70-71	36.95725922049306	38.0	38.0	38.0	36.0	38.0
72-73	36.9321863282467	38.0	38.0	38.0	36.0	38.0
74-75	36.95052448694841	38.0	38.0	38.0	36.0	38.0
76-77	36.937105043414164	38.0	38.0	38.0	36.0	38.0
78-79	36.92519600932789	38.0	38.0	38.0	36.0	38.0
80-81	36.898447202975795	38.0	38.0	38.0	35.0	38.0
82-83	36.88683949868855	38.0	38.0	38.0	35.0	38.0
84-85	36.86676793575037	38.0	38.0	38.0	35.0	38.0
86-87	36.82382703070833	38.0	38.0	38.0	35.0	38.0
88-89	36.805539050664535	38.0	38.0	38.0	35.0	38.0
90-91	36.80928861830693	38.0	38.0	38.0	35.0	38.0
92-93	36.77362656518498	38.0	38.0	38.0	35.0	38.0
94-95	36.71370345848078	38.0	38.0	38.0	34.5	38.0
96-97	36.726892329382395	38.0	38.0	38.0	34.5	38.0
98-99	36.75395265361914	38.0	38.0	38.0	35.0	38.0
100	35.13088229310614	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	18.0
19	42.0
20	79.0
21	228.0
22	718.0
23	1957.0
24	4354.0
25	8224.0
26	14147.0
27	22789.0
28	33801.0
29	48234.0
30	65754.0
31	85297.0
32	109174.0
33	145047.0
34	206676.0
35	342585.0
36	829818.0
37	5035950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.754124178043654	19.735176676130976	14.103105134063016	24.407594011762352
2	14.591609571437466	20.183766814222086	42.827568645477484	22.397054968862964
3	18.405725688978176	25.815306357344266	29.90317187836319	25.875796075314373
4	12.502895440208277	17.598942098528795	39.22130595388777	30.67685650737516
5	13.485377241152221	37.335564959443936	35.30748845723028	13.871569342173565
6	29.850523510637792	37.72491604088242	18.809070992381887	13.615489456097905
7	26.506372987911693	32.73346378330906	22.990967089251164	17.76919613952808
8	24.162270606008626	36.98141785195792	21.02478525574987	17.831526286283587
9	25.532671133636555	17.065965424957163	21.40464688138623	35.99671656002005
10-11	24.289310516425754	26.91243915621516	28.591197775496735	20.207052551862347
12-13	25.075267067065372	24.474935013453102	28.893099988051592	21.556697931429937
14-15	22.258900593984013	25.71346450510223	26.83866384295046	25.1889710579633
16-17	21.56403088987176	28.86680917528517	27.476099228443367	22.093060706399704
18-19	21.483382725361583	27.776764902908035	29.478413855208004	21.261438516522375
20-21	22.507176961927044	26.95861919293857	29.396815918423176	21.137387926711213
22-23	22.150560250484894	26.86334393996373	29.328745311104964	21.65735049844641
24-25	21.821435894853096	26.988024016721045	29.441902630831052	21.748637457594803
26-27	21.749365207239453	27.15778801225124	29.345993252578644	21.746853527930664
28-29	21.67922760659303	27.242163056350428	29.382015579424653	21.69659375763189
30-31	21.975890506760017	27.057053877839937	29.272194375537403	21.694861239862643
32-33	21.77548262482752	27.247650602144326	29.20014229345013	21.776724479578025
34-35	21.929145577748603	27.255751590548254	29.181062786660377	21.634040045042767
36-37	21.923035607635967	27.14969030096307	29.118093670803518	21.809180420597446
38-39	21.742463716407386	27.333925718518913	29.227170957735865	21.696439607337837
40-41	22.024693385035377	27.21077565842932	29.07090815715569	21.69362279937961
42-43	21.9031776718068	27.239206332418902	28.92904684616871	21.928569149605583
44-45	21.987913929976173	27.409533882187255	28.745960731464464	21.856591456372108
46-47	22.016345750520948	27.425145076688885	28.4713540778348	22.087155094955367
48-49	21.98107230055876	27.504096421202874	28.47642911039087	22.03840216784749
50-51	22.016513592261227	27.75331989198409	28.167307651676076	22.062858864078606
52-53	22.209398471661903	27.726052895727655	27.952980720787934	22.111567911822508
54-55	22.18163354154338	27.63341361585595	27.9961485452271	22.18880429737357
56-57	22.1928106420895	27.74743354232787	27.927184652335047	22.132571163247583
58-59	22.17757547375469	27.782657535575478	27.982274700167427	22.057492290502406
60-61	22.17542432864862	27.733150480310087	28.02335623037104	22.06806896067025
62-63	22.16336032923627	27.746016822213072	28.007997134048924	22.082625714501738
64-65	22.135725827942736	27.684660917894956	27.998448196825144	22.181165057337164
66-67	22.216952960801507	27.72131316877346	27.842251231718006	22.21948263870702
68-69	22.240231078890705	27.672003003462965	27.929678667570673	22.158087250075663
70-71	22.191681459690965	27.470078930688125	28.004987319263098	22.333252290357812
72-73	22.224172168677747	27.53254774068053	27.95975243945791	22.283527651183814
74-75	22.097412621440675	27.688927294998074	27.90338439854355	22.310275685017704
76-77	22.225250094904208	27.635496432980794	27.81157366872048	22.32767980339452
78-79	22.189511788091703	27.65679062153173	27.753476389456694	22.400221200919873
80-81	22.20104028547016	27.732248677239557	27.718418760570625	22.348292276719658
82-83	22.263109739632753	27.680576800106653	27.802439284645565	22.25387417561503
84-85	22.385734565605865	27.664042837426887	27.708929782764553	22.241292814202694
86-87	22.187272405033585	27.7037828534205	27.79304454298112	22.315900198564787
88-89	22.327514555874043	27.690109578625567	27.705764561031348	22.276611304469046
90-91	22.352814974317464	27.76481864700179	27.679398151802904	22.202968226877847
92-93	22.361863848077963	27.746031699734736	27.673460265590304	22.218644186596997
94-95	22.223133078424596	27.84980808157473	27.662329312711716	22.264729527288964
96-97	22.33235930374874	27.911213507415773	27.76632859252024	21.990098596315246
98-99	23.351359592408645	29.872686184179216	27.951332730486182	18.824621492925957
100	22.30913416096487	30.793972833740813	21.89871969689062	24.998173308403697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2239.0
1	1889.0
2	1701.5
3	2318.0
4	3461.0
5	4843.5
6	5772.0
7	5921.0
8	5698.5
9	5712.0
10	6006.5
11	7181.5
12	9217.0
13	13693.0
14	20876.0
15	28590.0
16	34606.0
17	37077.5
18	37271.5
19	34154.0
20	30236.0
21	27824.5
22	27380.5
23	30876.5
24	37178.0
25	46311.0
26	58953.5
27	73730.0
28	87922.0
29	102969.5
30	117565.5
31	131234.0
32	147504.5
33	164011.0
34	180308.0
35	194874.5
36	209351.5
37	224014.5
38	233942.0
39	242196.0
40	252300.0
41	260074.0
42	264594.0
43	269759.5
44	275172.5
45	280799.5
46	283964.0
47	283959.5
48	283048.5
49	279674.5
50	274487.0
51	267822.5
52	256571.5
53	242650.0
54	228368.0
55	213128.0
56	196525.5
57	177868.5
58	158520.5
59	139061.5
60	118063.5
61	96036.0
62	75585.0
63	58385.5
64	44445.0
65	33226.5
66	24742.0
67	18816.0
68	14439.0
69	10739.5
70	7785.5
71	5583.5
72	3899.0
73	2612.0
74	1697.0
75	1104.5
76	679.5
77	404.0
78	272.5
79	171.5
80	109.0
81	73.5
82	54.5
83	40.0
84	27.0
85	16.0
86	11.5
87	10.5
88	7.5
89	4.0
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0047592338122426185
6	4.025937968060221E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	4.332049578574607E-5
34-35	1.2303587697222893E-4
36-37	1.2347128028968396E-4
38-39	1.679176286643277E-4
40-41	1.1014379566240515E-4
42-43	1.6248156018929986E-4
44-45	1.63604376952516E-4
46-47	5.247910544416741E-5
48-49	3.0233193915479026E-4
50-51	3.5086817371666345E-4
52-53	4.3842420495423964E-4
54-55	0.0017887850610583582
56-57	0.002855091916017861
58-59	0.0017058989356291223
60-61	0.003090065668649406
62-63	0.001612750114988285
64-65	0.0023889411810506934
66-67	6.080919525840706E-4
68-69	5.070076222053964E-4
70-71	2.887508604775642E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	835.0
20-21	2646.0
22-23	1718.0
24-25	1662.0
26-27	2471.0
28-29	4835.0
30-31	12096.0
32-33	15445.0
34-35	21250.0
36-37	33309.0
38-39	39836.0
40-41	38619.0
42-43	43730.0
44-45	53295.0
46-47	54876.0
48-49	51308.0
50-51	65807.0
52-53	43037.0
54-55	46180.0
56-57	49235.0
58-59	49691.0
60-61	48942.0
62-63	45969.0
64-65	48446.0
66-67	52071.0
68-69	53590.0
70-71	52901.0
72-73	49359.0
74-75	49055.0
76-77	52135.0
78-79	54011.0
80-81	54301.0
82-83	50157.0
84-85	48803.0
86-87	51002.0
88-89	55201.0
90-91	57717.0
92-93	56595.0
94-95	62836.0
96-97	209567.0
98-99	476083.0
100-101	4694279.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.26622492071486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69595047733206	76.48035087877497
2	7.3147856552259	12.474083178506204
3	1.7174450032315542	4.393201558034987
4	0.591313653515781	2.0167633191746495
5	0.2710668641149876	1.1556424102090692
6	0.1385053544244522	0.7085897221847202
7	0.08453963513257894	0.5045862880750752
8	0.04573151554986297	0.3119482952671828
9	0.032913035942053206	0.2525733292912813
>10	0.10429637549017286	1.3214292073289564
>50	0.0025443383119478093	0.14115891478770295
>100	7.493051336363614E-4	0.10940395944874573
>500	1.0585780770245459E-4	0.07053888254489211
>1k	5.2928787294833065E-5	0.05973005637164943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.875669977185872E-5	0.0	0.0	0.0
3	0.0	2.875669977185872E-5	0.0	0.0	0.0
4	0.0	1.1502679908743489E-4	0.0	0.0	1.437834988592936E-5
5	0.0	1.1502679908743489E-4	0.0	0.0	1.437834988592936E-5
6	5.751339954371744E-5	1.1502679908743489E-4	0.0	0.0	1.437834988592936E-5
7	5.751339954371744E-5	1.1502679908743489E-4	0.0	0.0	1.437834988592936E-5
8	5.751339954371744E-5	1.2940514897336424E-4	0.0	0.0	1.437834988592936E-5
9	5.751339954371744E-5	2.444319480607991E-4	0.0	2.875669977185872E-5	1.437834988592936E-5
10-11	5.751339954371744E-5	2.444319480607991E-4	0.0	6.470257448668212E-5	1.437834988592936E-5
12-13	5.751339954371744E-5	2.444319480607991E-4	0.0	7.18917494296468E-5	1.437834988592936E-5
14-15	5.751339954371744E-5	3.2351287243341065E-4	0.0	7.908092437261148E-5	1.437834988592936E-5
16-17	1.1502679908743489E-4	3.3070204737637533E-4	0.0	1.0064844920150553E-4	1.437834988592936E-5
18-19	1.1502679908743489E-4	3.3789122231934E-4	0.0	1.1502679908743489E-4	1.437834988592936E-5
20-21	1.1502679908743489E-4	3.4508039726230465E-4	0.0	1.2940514897336424E-4	1.437834988592936E-5
22-23	1.1502679908743489E-4	3.8821544692009275E-4	0.0	1.3659432391632893E-4	1.437834988592936E-5
24-25	1.1502679908743489E-4	4.241613216349161E-4	0.0	1.437834988592936E-4	1.437834988592936E-5
26-27	1.1502679908743489E-4	4.457288464638102E-4	0.0	2.0129689840301106E-4	1.437834988592936E-5
28-29	1.2940514897336424E-4	4.457288464638102E-4	0.0	3.8821544692009275E-4	1.437834988592936E-5
30-31	1.2940514897336424E-4	4.744855462356689E-4	0.0	0.0011071329412165609	1.437834988592936E-5
32-33	1.2940514897336424E-4	4.744855462356689E-4	0.0	0.002480265355322815	1.437834988592936E-5
34-35	1.2940514897336424E-4	5.319989457793863E-4	0.0	0.0043925858901514205	1.437834988592936E-5
36-37	1.2940514897336424E-4	5.895123453231038E-4	0.0	0.007102904843649104	1.437834988592936E-5
38-39	1.2940514897336424E-4	6.038906952090332E-4	0.0	0.01155300413334424	1.437834988592936E-5
40-41	1.2940514897336424E-4	6.757824446386799E-4	0.0	0.01586650909912305	1.437834988592936E-5
42-43	1.2940514897336424E-4	6.97349969467574E-4	0.0	0.020309419213875222	2.156752482889404E-5
44-45	1.2940514897336424E-4	7.620525439542561E-4	0.0	0.025104598900832664	2.875669977185872E-5
46-47	1.2940514897336424E-4	7.908092437261149E-4	0.0	0.030712155356345115	2.875669977185872E-5
48-49	1.2940514897336424E-4	7.908092437261149E-4	0.0	0.036621657159462084	2.875669977185872E-5
50-51	1.2940514897336424E-4	8.483226432698324E-4	0.0	0.04254553731246498	2.875669977185872E-5
52-53	1.437834988592936E-4	8.483226432698324E-4	0.0	0.048203417992578185	2.875669977185872E-5
54-55	1.437834988592936E-4	8.483226432698324E-4	0.0	0.05355935332508687	2.875669977185872E-5
56-57	1.437834988592936E-4	8.483226432698324E-4	0.0	0.05915253143071339	2.875669977185872E-5
58-59	1.437834988592936E-4	8.55511818212797E-4	0.0	0.06534241105660599	2.875669977185872E-5
60-61	1.437834988592936E-4	8.627009931557617E-4	0.0	0.07108656183603476	2.875669977185872E-5
62-63	1.5816184874522298E-4	8.77079343041691E-4	0.0	0.07629152449474119	2.875669977185872E-5
64-65	1.5816184874522298E-4	9.92106142129126E-4	0.0	0.08151086550333356	2.875669977185872E-5
66-67	1.869185485170817E-4	9.992953170720907E-4	0.0	0.086917125060443	2.875669977185872E-5
68-69	1.869185485170817E-4	0.00101367366695802	0.0	0.0922874387428376	2.875669977185872E-5
70-71	2.0129689840301106E-4	0.0010208628419009847	0.0	0.0970107266803654	2.875669977185872E-5
72-73	2.0848607334597575E-4	0.0010280520168439492	0.0	0.10059812497690478	4.313504965778808E-5
74-75	2.1567524828894043E-4	0.001035241191786914	0.0	0.1032581197058017	4.313504965778808E-5
76-77	2.1567524828894043E-4	0.0010496195416728432	0.0	0.10574557423606748	4.313504965778808E-5
78-79	2.1567524828894043E-4	0.0010639978915587727	0.0	0.10730562519869083	4.313504965778808E-5
80-81	2.1567524828894043E-4	0.0010639978915587727	0.0	0.10790232671895689	5.751339954371744E-5
82-83	2.1567524828894043E-4	0.0010639978915587727	0.0	0.10830492051576292	7.18917494296468E-5
84-85	2.2286442323190512E-4	0.0010639978915587727	0.0	0.10858529833853853	7.18917494296468E-5
86-87	2.3724277311783443E-4	0.0010639978915587727	0.0	0.10867875761279708	7.18917494296468E-5
88	2.444319480607991E-4	0.0010927545913306314	0.0	0.10868594678774005	7.18917494296468E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10790	0.0	49.723114	1
GTATCAA	19775	0.0	38.3484	1
TCAACGC	23680	0.0	31.389072	4
ATCAACG	23750	0.0	31.296556	3
CAACGCA	24145	0.0	30.748951	5
TATCAAC	24610	0.0	30.395048	2
AACGCAG	24885	0.0	29.867622	6
ACGCAGA	28490	0.0	26.103207	7
CGCAGAG	28970	0.0	25.64103	8
GTGGTAT	5035	0.0	25.273037	1
TGGTATC	5005	0.0	23.878437	2
GCAGAGT	32865	0.0	22.57602	9
GTACATG	24590	0.0	20.43719	1
TACATGG	24150	0.0	20.43572	2
GAGTACT	19045	0.0	20.34815	12-13
ACATGGG	24285	0.0	19.737946	3
CAGAGTA	32670	0.0	19.427841	10-11
AGAGTAC	30360	0.0	18.768007	10-11
GTACTTT	20870	0.0	18.253786	14-15
CATGGGG	16580	0.0	17.553728	4
>>END_MODULE