FastQCFastQC Report
Fri 27 May 2016
SRR2138747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8768982
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109550.050.5802081
GTATCAA198800.038.191691
TCAACGC237100.031.3484214
ATCAACG240000.030.9336383
CAACGCA246300.030.2300725
TATCAAC251000.029.8360792
AACGCAG253700.029.3292856
CTTATAC61600.029.0960331
ACGCAGA291700.025.5677537
CGCAGAG299100.024.9351838
TATACAC97250.022.6044833
GTGGTAT51050.021.9960121
GCAGAGT348150.021.4345329
GTACATG244600.021.011491
TACATGG241800.020.6653822
TGGTATC51150.020.5176222
ACATGGG241000.020.2859693
GAGTACT210950.020.18524412-13
TTATACA99500.019.6192172
TATGCCG51750.019.58928342-43
Produced by FastQC (version 0.11.5)