##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8768982
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.190281380438456	33.0	32.0	34.0	31.0	34.0
2	31.98215391478737	33.0	32.0	33.0	30.0	34.0
3	32.025814855133696	33.0	31.0	33.0	29.0	34.0
4	32.45607688554954	33.0	33.0	34.0	31.0	34.0
5	32.78989351329493	33.0	33.0	34.0	31.0	34.0
6	36.78775004897946	38.0	37.0	38.0	35.0	38.0
7	37.077880990062475	38.0	38.0	38.0	36.0	38.0
8	37.118376682720985	38.0	38.0	38.0	36.0	38.0
9	37.21095641432495	38.0	38.0	38.0	36.0	38.0
10-11	37.25786687667964	38.0	38.0	38.0	36.5	38.0
12-13	37.295602784907075	38.0	38.0	38.0	37.0	38.0
14-15	37.32117011986112	38.0	38.0	38.0	37.0	38.0
16-17	37.318292590861745	38.0	38.0	38.0	37.0	38.0
18-19	37.19119978807118	38.0	38.0	38.0	36.5	38.0
20-21	37.30954533824026	38.0	38.0	38.0	37.0	38.0
22-23	37.331419599744976	38.0	38.0	38.0	37.0	38.0
24-25	37.33896216773992	38.0	38.0	38.0	37.0	38.0
26-27	37.30749608777916	38.0	38.0	38.0	37.0	38.0
28-29	37.28603124468441	38.0	38.0	38.0	37.0	38.0
30-31	37.28827731966423	38.0	38.0	38.0	37.0	38.0
32-33	37.27131430288999	38.0	38.0	38.0	37.0	38.0
34-35	37.256730065440465	38.0	38.0	38.0	37.0	38.0
36-37	37.23525268581031	38.0	38.0	38.0	37.0	38.0
38-39	37.19292089630436	38.0	38.0	38.0	36.5	38.0
40-41	37.19902881727085	38.0	38.0	38.0	37.0	38.0
42-43	37.174903793447356	38.0	38.0	38.0	36.0	38.0
44-45	37.144527444427595	38.0	38.0	38.0	36.0	38.0
46-47	37.10706157620815	38.0	38.0	38.0	36.0	38.0
48-49	37.07386084511823	38.0	38.0	38.0	36.0	38.0
50-51	37.04869235385462	38.0	38.0	38.0	36.0	38.0
52-53	37.02986067878214	38.0	38.0	38.0	36.0	38.0
54-55	37.01417149285126	38.0	38.0	38.0	36.0	38.0
56-57	37.00145460132371	38.0	38.0	38.0	36.0	38.0
58-59	36.99147110015562	38.0	38.0	38.0	36.0	38.0
60-61	36.98929460246339	38.0	38.0	38.0	36.0	38.0
62-63	36.981915131842456	38.0	38.0	38.0	36.0	38.0
64-65	36.98567495576805	38.0	38.0	38.0	36.0	38.0
66-67	36.97680826285909	38.0	38.0	38.0	36.0	38.0
68-69	36.97608250333237	38.0	38.0	38.0	36.0	38.0
70-71	36.965186705960775	38.0	38.0	38.0	36.0	38.0
72-73	36.933485314844916	38.0	38.0	38.0	36.0	38.0
74-75	36.950472233712965	38.0	38.0	38.0	36.0	38.0
76-77	36.93798004481609	38.0	38.0	38.0	36.0	38.0
78-79	36.92809775285453	38.0	38.0	38.0	36.0	38.0
80-81	36.9034351755056	38.0	38.0	38.0	35.5	38.0
82-83	36.893398529165665	38.0	38.0	38.0	35.0	38.0
84-85	36.875168107678334	38.0	38.0	38.0	35.0	38.0
86-87	36.836632741239015	38.0	38.0	38.0	35.0	38.0
88-89	36.81870787390288	38.0	38.0	38.0	35.0	38.0
90-91	36.82894905465135	38.0	38.0	38.0	35.0	38.0
92-93	36.794043818451044	38.0	38.0	38.0	35.0	38.0
94-95	36.73572301708427	38.0	38.0	38.0	35.0	38.0
96-97	36.75234890060045	38.0	38.0	38.0	35.0	38.0
98-99	36.78194396586775	38.0	38.0	38.0	35.0	38.0
100	35.2188985190142	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	22.0
19	49.0
20	87.0
21	226.0
22	735.0
23	2055.0
24	4818.0
25	9476.0
26	16528.0
27	26868.0
28	40430.0
29	58851.0
30	80155.0
31	106520.0
32	139597.0
33	183396.0
34	260421.0
35	431177.0
36	1041165.0
37	6366404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56744762390891	19.365634460191615	13.921764236715276	25.145153679184197
2	14.747264847846647	19.909323568003675	42.39933438111744	22.944077203032233
3	18.54003121456972	25.130317293387076	29.712080604111176	26.61757088793203
4	12.738833310411628	17.500298210214137	38.48942784920758	31.271440630166648
5	13.898585478301023	36.813111682015276	35.14292618834112	14.145376651342579
6	30.521635999749115	37.152669361782195	18.48563396985956	13.840060668609128
7	26.973872223708522	32.164144024927865	22.543814093813854	18.31816965754976
8	24.34197036782605	36.80593710877728	20.870278898964557	17.981813624432117
9	25.463594291788944	16.96686114762238	21.017126047242428	36.55241851334625
10-11	24.48173573625764	26.54436968852257	28.471075661918338	20.50281891330145
12-13	25.305327648370568	23.918610160142315	28.6821337232836	22.09392846820352
14-15	22.42871079155544	25.27342542574642	26.28192339098902	26.01594039170912
16-17	22.105085858312858	28.448940823461605	26.991496846498258	22.45447647172728
18-19	22.143881695731615	27.392204705175583	28.722735432687625	21.741178166405177
20-21	22.757224015278155	26.63107442877834	28.75769648264977	21.854005073293738
22-23	22.540207563042507	26.636036840412896	28.607703842466904	22.216051754077693
24-25	22.36535399537411	26.774692972899178	28.720635491082263	22.13931754064445
26-27	22.34369655924292	26.89223160987792	28.668726776217134	22.095345054662026
28-29	22.209290020409007	26.926118252441306	28.69509855796315	22.169493169186534
30-31	22.613988617480963	26.699780279758645	28.61233122405327	22.073899878707117
32-33	22.158449849265356	26.995608514703072	28.612830703821384	22.233110932210185
34-35	22.423624411901464	26.94034836453335	28.61598720266418	22.020040020901003
36-37	22.292626536035325	26.94263417647189	28.593163942152522	22.17157534534026
38-39	22.154063791208188	26.878994026614233	28.780611363700693	22.186330818476886
40-41	22.424926005631534	26.717426843114634	28.663279416009637	22.194367735244196
42-43	22.394841639567666	26.798586602142795	28.509197780894375	22.297373977395164
44-45	22.339701674920835	26.931446330272536	28.43674251103004	22.29210948377659
46-47	22.527919589923766	26.86977131934471	28.103989282286683	22.498319808444844
48-49	22.42681792714815	26.970619123115036	28.084655765372773	22.517907184364034
50-51	22.337553347651045	27.127177725664602	28.099556021203476	22.43571290548088
52-53	22.550478573063895	27.025301720667862	28.09241170797796	22.331807998290284
54-55	22.453978287254333	26.965131240741357	27.999555889483403	22.581334582520903
56-57	22.54042611388723	27.1464761585437	27.865483294755244	22.44761443281383
58-59	22.407248249879334	27.24622764250828	28.01638752576176	22.33013658185062
60-61	22.550321361754612	27.36033944229877	27.85038805284711	22.238951143099506
62-63	22.399862213568593	27.525120242030965	27.797253456298577	22.27776408810187
64-65	22.39543995036234	27.45985087241468	27.770578577916417	22.374130599306564
66-67	22.455145673334417	27.424380373982565	27.687579220446935	22.432894732236083
68-69	22.398321949507544	27.390800923295167	27.787910079626727	22.422967047570562
70-71	22.468458706390372	27.16711974052957	27.825625415051146	22.53879613802891
72-73	22.545847234496648	27.076474434684204	27.758949653258135	22.61872867756101
74-75	22.397770529237835	27.230211925043307	27.742839128566764	22.629178417152087
76-77	22.601477108100337	27.144894207968328	27.65831717914194	22.595311504789393
78-79	22.539759628560834	27.130696932080156	27.671969293932406	22.6575741454266
80-81	22.53834079908941	27.219466115065643	27.609306830238168	22.632886255606774
82-83	22.59444193249639	27.19210479718092	27.6564226833674	22.55703058695529
84-85	22.66955332059022	27.186941816304394	27.59031727806278	22.553187585042604
86-87	22.53063157057479	27.187441698016794	27.682047801206703	22.599878930201708
88-89	22.584365635902177	27.19704481310537	27.592204210566827	22.626385340425625
90-91	22.630718595052283	27.235313964737035	27.579522071227963	22.554445368982723
92-93	22.661064782785463	27.229603678362924	27.576623371787807	22.532708167063802
94-95	22.563509017763696	27.28802918876981	27.562710089405474	22.58575170406102
96-97	22.677238952522647	27.36514249578204	27.60558545349471	22.3520330982006
98-99	23.76893090832041	29.317910795020836	27.80303370328541	19.110124593373342
100	22.65534044607384	30.144415010527	21.78500928182303	25.415235261576136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1920.0
1	1767.0
2	2358.5
3	3344.0
4	4083.5
5	5449.0
6	6869.5
7	9225.0
8	12311.0
9	13849.0
10	13386.0
11	12757.0
12	13816.0
13	16646.0
14	21581.0
15	27107.5
16	30959.0
17	32887.5
18	32621.0
19	30163.5
20	27207.5
21	25333.0
22	25710.0
23	29180.5
24	36158.5
25	46897.0
26	60729.5
27	75719.0
28	91053.5
29	108291.0
30	125139.5
31	142445.5
32	162109.0
33	181557.5
34	200085.0
35	217280.0
36	236617.0
37	256214.0
38	272091.5
39	286826.0
40	302272.0
41	316257.0
42	327819.0
43	345220.5
44	359440.0
45	366063.0
46	368363.0
47	366180.0
48	366475.0
49	364885.0
50	361376.0
51	355364.0
52	343768.0
53	327711.0
54	311349.0
55	293686.0
56	271875.0
57	247110.0
58	220149.5
59	192101.5
60	163312.0
61	134294.5
62	106914.5
63	83152.5
64	62459.0
65	45998.5
66	34009.0
67	25657.0
68	19332.5
69	14087.0
70	10223.0
71	7344.5
72	5158.0
73	3525.0
74	2339.0
75	1487.5
76	925.0
77	567.0
78	367.5
79	233.5
80	149.0
81	98.0
82	63.0
83	38.0
84	26.0
85	20.5
86	17.5
87	13.0
88	7.5
89	3.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.004960667042080825
6	3.649226329806584E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.701916140322787E-6
14-15	5.701916140322787E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	5.146671267409544E-5
34-35	1.3180587516092496E-4
36-37	1.6672106180849926E-4
38-39	1.559628316311338E-4
40-41	9.290842208458743E-5
42-43	1.4594441771067032E-4
44-45	1.409962939124145E-4
46-47	5.326892449567942E-5
48-49	3.160326901829573E-4
50-51	4.323397325618471E-4
52-53	3.8076820771618916E-4
54-55	0.002135335105560078
56-57	0.003357700764158775
58-59	0.0019444206793028083
60-61	0.0034765480402966123
62-63	0.0021238908778698842
64-65	0.002903441019436013
66-67	6.810167286985924E-4
68-69	7.514867877061386E-4
70-71	3.695813777669569E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	7.023182612791084E-6
90-91	0.0
92-93	0.0
94-95	7.23339339460982E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	78.0
20-21	278.0
22-23	664.0
24-25	665.0
26-27	1838.0
28-29	4609.0
30-31	13440.0
32-33	17188.0
34-35	24102.0
36-37	38643.0
38-39	45810.0
40-41	44782.0
42-43	51115.0
44-45	62032.0
46-47	63379.0
48-49	59693.0
50-51	54928.0
52-53	52900.0
54-55	57424.0
56-57	60154.0
58-59	60549.0
60-61	59779.0
62-63	58213.0
64-65	63473.0
66-67	70080.0
68-69	72212.0
70-71	79598.0
72-73	63388.0
74-75	62485.0
76-77	65255.0
78-79	67121.0
80-81	66562.0
82-83	63133.0
84-85	62448.0
86-87	64899.0
88-89	67782.0
90-91	68323.0
92-93	68891.0
94-95	82052.0
96-97	273934.0
98-99	608044.0
100-101	5967039.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.48752537180359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.35384773156716	77.24126850392425
2	6.822382348300245	11.66457168192924
3	1.5430571407063458	3.9573640939882924
4	0.5544677807553944	1.896003139006976
5	0.2699942912872825	1.1540571913331843
6	0.1454374956445401	0.7459854959354515
7	0.08514125657330253	0.5094960732048207
8	0.05040383306447709	0.344711916634851
9	0.037022787514808406	0.28484878383063694
>10	0.13345601630855533	1.8191827386595516
>50	0.0037623074259402144	0.2216967716607172
>100	9.817703514769613E-4	0.1228927902319961
>500	2.262025600577085E-5	0.014213060362693703
>1k	2.2620244424950268E-5	0.023707759297322543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.280766456129115E-5	0.0	2.280766456129115E-5	0.0
3	0.0	2.280766456129115E-5	0.0	2.280766456129115E-5	0.0
4	0.0	6.842299368387346E-5	0.0	4.56153291225823E-5	0.0
5	0.0	6.842299368387346E-5	0.0	4.56153291225823E-5	0.0
6	0.0	6.842299368387346E-5	0.0	4.56153291225823E-5	0.0
7	0.0	6.842299368387346E-5	0.0	4.56153291225823E-5	0.0
8	0.0	7.982682596451903E-5	0.0	4.56153291225823E-5	1.1403832280645575E-5
9	0.0	2.0526898105162035E-4	0.0	4.56153291225823E-5	3.421149684193673E-5
10-11	0.0	2.0526898105162035E-4	0.0	6.272107754355067E-5	3.421149684193673E-5
12-13	0.0	2.0526898105162035E-4	0.0	9.693257438548739E-5	3.421149684193673E-5
14-15	5.701916140322787E-6	2.736919747354938E-4	0.0	1.0833640666613297E-4	5.131724526290509E-5
16-17	1.1403832280645575E-5	2.736919747354938E-4	0.0	1.1974023894677854E-4	5.701916140322788E-5
18-19	1.1403832280645575E-5	3.0790347157743053E-4	0.0	1.2544215508710133E-4	6.272107754355067E-5
20-21	1.1403832280645575E-5	3.421149684193673E-4	0.0	1.2544215508710133E-4	6.842299368387346E-5
22-23	1.1403832280645575E-5	3.7062454912098117E-4	0.0	1.368459873677469E-4	7.982682596451902E-5
24-25	1.1403832280645575E-5	3.76326465261304E-4	0.0	1.7675940035000642E-4	9.12306582451646E-5
26-27	1.1403832280645575E-5	3.76326465261304E-4	0.0	2.1097089719194313E-4	1.0263449052581018E-4
28-29	1.1403832280645575E-5	3.76326465261304E-4	0.0	3.7632646526130395E-4	1.311440712274241E-4
30-31	1.1403832280645575E-5	4.105379621032407E-4	0.0	9.69325743854874E-4	1.4254790350806971E-4
32-33	1.1403832280645575E-5	4.219417943838863E-4	0.0	0.002172430049462982	1.5965365192903807E-4
34-35	1.1403832280645575E-5	4.390475428048547E-4	0.0	0.003985639382085629	1.824613164903292E-4
36-37	1.1403832280645575E-5	4.56153291225823E-4	0.0	0.006739664877861535	1.824613164903292E-4
38-39	1.1403832280645575E-5	4.84662871927437E-4	0.0	0.01114724605433105	1.8816323263065197E-4
40-41	2.280766456129115E-5	5.473839494709876E-4	0.0	0.015902644115360253	2.0526898105162035E-4
42-43	2.280766456129115E-5	5.530858656113104E-4	0.0	0.020315927207970094	2.0526898105162035E-4
44-45	2.280766456129115E-5	6.158069431548611E-4	0.0	0.025099834849700912	2.280766456129115E-4
46-47	3.421149684193672E-5	6.728261045580889E-4	0.0	0.031143865958443068	2.67990058595171E-4
48-49	4.56153291225823E-5	6.728261045580889E-4	0.0	0.03697692616999328	2.736919747354938E-4
50-51	4.56153291225823E-5	7.355471821016396E-4	0.0	0.042809986381543486	2.850958070161394E-4
52-53	4.56153291225823E-5	7.412490982419624E-4	0.0	0.04823821054713079	3.0220155543710775E-4
54-55	5.131724526290509E-5	7.52652930522608E-4	0.0	0.05364932896429711	3.1930730385807614E-4
56-57	5.701916140322788E-5	7.52652930522608E-4	0.0	0.05871833241304407	3.1930730385807614E-4
58-59	5.701916140322788E-5	7.640567628032536E-4	0.0	0.06361627837758135	3.1930730385807614E-4
60-61	5.701916140322788E-5	7.925663435048675E-4	0.0	0.06828044578036538	3.1930730385807614E-4
62-63	5.701916140322788E-5	8.267778403468042E-4	0.0	0.07272223845367684	3.307111361387217E-4
64-65	5.701916140322788E-5	9.009027501710005E-4	0.0	0.07708420430102378	3.307111361387217E-4
66-67	5.701916140322788E-5	9.12306582451646E-4	0.0	0.0816970544585449	3.421149684193673E-4
68-69	5.701916140322788E-5	9.408161631532599E-4	0.0	0.08572260725361279	3.421149684193673E-4
70-71	6.842299368387346E-5	9.579219115742284E-4	0.0	0.08981088112622423	3.4781688455969007E-4
72-73	6.842299368387346E-5	9.579219115742284E-4	0.0	0.09299255033252435	3.649226329806584E-4
74-75	6.842299368387346E-5	9.69325743854874E-4	0.0	0.0954614800212841	3.820283814016268E-4
76-77	6.842299368387346E-5	9.978353245564879E-4	0.0	0.0980102365360084	3.934322136822724E-4
78-79	6.842299368387346E-5	0.0010149410729774562	0.0	0.09974361904266653	3.9913412982259516E-4
80-81	6.842299368387346E-5	0.0010149410729774562	0.0	0.10035942598582138	3.9913412982259516E-4
82-83	6.842299368387346E-5	0.0010149410729774562	0.0	0.10079847352862624	4.219417943838863E-4
84-85	6.842299368387346E-5	0.0010263449052581018	0.0	0.10099233867739721	4.219417943838863E-4
86-87	6.842299368387346E-5	0.0010377487375387473	0.0	0.10107786741950206	4.219417943838863E-4
88	6.842299368387346E-5	0.0010719602343806842	0.0	0.10110637700020367	4.219417943838863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10955	0.0	50.580208	1
GTATCAA	19880	0.0	38.19169	1
TCAACGC	23710	0.0	31.348421	4
ATCAACG	24000	0.0	30.933638	3
CAACGCA	24630	0.0	30.230072	5
TATCAAC	25100	0.0	29.836079	2
AACGCAG	25370	0.0	29.329285	6
CTTATAC	6160	0.0	29.096033	1
ACGCAGA	29170	0.0	25.567753	7
CGCAGAG	29910	0.0	24.935183	8
TATACAC	9725	0.0	22.604483	3
GTGGTAT	5105	0.0	21.996012	1
GCAGAGT	34815	0.0	21.434532	9
GTACATG	24460	0.0	21.01149	1
TACATGG	24180	0.0	20.665382	2
TGGTATC	5115	0.0	20.517622	2
ACATGGG	24100	0.0	20.285969	3
GAGTACT	21095	0.0	20.185244	12-13
TTATACA	9950	0.0	19.619217	2
TATGCCG	5175	0.0	19.589283	42-43
>>END_MODULE