FastQCFastQC Report
Fri 27 May 2016
SRR2138746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514004
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105550.050.22711
GTATCAA188800.037.2417531
TCAACGC226200.030.645574
ATCAACG228300.030.3069973
CAACGCA232800.029.7211655
TATCAAC238700.029.2576122
AACGCAG241300.028.7976576
CTTATAC63250.026.2569711
ACGCAGA278850.024.888827
CGCAGAG286000.024.2364398
GTGGTAT52400.023.6262651
TGGTATC51400.022.8270742
TATACAC93800.021.1083493
GCAGAGT337750.020.5612479
GTACATG239850.020.0350271
TACATGG240650.019.4485992
GAGTACT199500.019.32918412-13
CAGAGTA326200.018.61819610-11
ACATGGG244550.018.4505143
AGAGTAC302700.018.2111510-11
Produced by FastQC (version 0.11.5)