##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8514004
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.264994472635905	33.0	33.0	34.0	31.0	34.0
2	32.02342705030441	33.0	32.0	33.0	30.0	34.0
3	32.071582066440186	33.0	33.0	33.0	29.0	34.0
4	32.4754632485491	33.0	33.0	34.0	31.0	34.0
5	32.79933565922685	33.0	33.0	34.0	31.0	34.0
6	36.8029179925215	38.0	37.0	38.0	35.0	38.0
7	37.081949808809114	38.0	38.0	38.0	36.0	38.0
8	37.12393945316445	38.0	38.0	38.0	36.0	38.0
9	37.21382090024858	38.0	38.0	38.0	36.0	38.0
10-11	37.25758738191807	38.0	38.0	38.0	36.5	38.0
12-13	37.29353744724574	38.0	38.0	38.0	37.0	38.0
14-15	37.317695645902916	38.0	38.0	38.0	37.0	38.0
16-17	37.3139414193369	38.0	38.0	38.0	37.0	38.0
18-19	37.18499315950521	38.0	38.0	38.0	36.5	38.0
20-21	37.30403265557037	38.0	38.0	38.0	37.0	38.0
22-23	37.324692424974884	38.0	38.0	38.0	37.0	38.0
24-25	37.3306101346055	38.0	38.0	38.0	37.0	38.0
26-27	37.30022182521089	38.0	38.0	38.0	37.0	38.0
28-29	37.277081663245696	38.0	38.0	38.0	37.0	38.0
30-31	37.2788819066373	38.0	38.0	38.0	37.0	38.0
32-33	37.26391972285647	38.0	38.0	38.0	37.0	38.0
34-35	37.24863512708008	38.0	38.0	38.0	37.0	38.0
36-37	37.226276569791395	38.0	38.0	38.0	37.0	38.0
38-39	37.185237433737825	38.0	38.0	38.0	36.5	38.0
40-41	37.19288449609276	38.0	38.0	38.0	37.0	38.0
42-43	37.169675872506346	38.0	38.0	38.0	36.0	38.0
44-45	37.13902880472943	38.0	38.0	38.0	36.0	38.0
46-47	37.105021632681726	38.0	38.0	38.0	36.0	38.0
48-49	37.07584733046366	38.0	38.0	38.0	36.0	38.0
50-51	37.05220939874747	38.0	38.0	38.0	36.0	38.0
52-53	37.03384951754626	38.0	38.0	38.0	36.0	38.0
54-55	37.01764193041577	38.0	38.0	38.0	36.0	38.0
56-57	37.005772852284004	38.0	38.0	38.0	36.0	38.0
58-59	36.99318420597437	38.0	38.0	38.0	36.0	38.0
60-61	36.98884989748777	38.0	38.0	38.0	36.0	38.0
62-63	36.983342194405964	38.0	38.0	38.0	36.0	38.0
64-65	36.984148577575795	38.0	38.0	38.0	36.0	38.0
66-67	36.97191771673957	38.0	38.0	38.0	36.0	38.0
68-69	36.97342062819004	38.0	38.0	38.0	36.0	38.0
70-71	36.96137216511013	38.0	38.0	38.0	36.0	38.0
72-73	36.93061993597575	38.0	38.0	38.0	36.0	38.0
74-75	36.949479839524656	38.0	38.0	38.0	36.0	38.0
76-77	36.93797911303323	38.0	38.0	38.0	36.0	38.0
78-79	36.92614367088228	38.0	38.0	38.0	36.0	38.0
80-81	36.90238542820752	38.0	38.0	38.0	35.0	38.0
82-83	36.888279739522886	38.0	38.0	38.0	35.0	38.0
84-85	36.87019067685165	38.0	38.0	38.0	35.0	38.0
86-87	36.831358649228775	38.0	38.0	38.0	35.0	38.0
88-89	36.814057987778654	38.0	38.0	38.0	35.0	38.0
90-91	36.8220891708917	38.0	38.0	38.0	35.0	38.0
92-93	36.78892075759103	38.0	38.0	38.0	35.0	38.0
94-95	36.72729110451245	38.0	38.0	38.0	34.5	38.0
96-97	36.74534802955468	38.0	38.0	38.0	35.0	38.0
98-99	36.772909454388525	38.0	38.0	38.0	35.0	38.0
100	35.202284424795764	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	22.0
19	37.0
20	74.0
21	235.0
22	763.0
23	2119.0
24	4829.0
25	9736.0
26	16536.0
27	26881.0
28	40132.0
29	57664.0
30	78321.0
31	103161.0
32	135551.0
33	178342.0
34	253178.0
35	415410.0
36	1005338.0
37	6185670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51644749051093	19.392426876942974	13.878957538662185	25.212168093883914
2	14.72822892730612	19.87610059849631	42.420792849051985	22.974877625145584
3	18.561748385366037	25.078482462540542	29.740683701816444	26.61908545027698
4	12.793064226890191	17.40639304374299	38.42701976649294	31.373522962873874
5	13.888948270876142	36.77371946920804	35.19776198556242	14.139570274353394
6	30.592493209103015	37.083541863132965	18.468232978477374	13.855731949286648
7	26.989137776907057	32.13789095446643	22.549627948216603	18.32334332040992
8	24.370707366357827	36.79643561360788	20.82736865051978	18.00548836951451
9	25.463225058386158	16.967175491108534	21.045092297349164	36.524507153156144
10-11	24.554492809728536	26.490086215604315	28.41966012700957	20.535760847657578
12-13	25.302127254234748	23.894417003704543	28.618146562894882	22.18530917916583
14-15	22.410801075498675	25.245513156911837	26.25149694550296	26.09218882208653
16-17	22.15636144873787	28.371157683270997	26.956124286528404	22.51635658146273
18-19	22.148956002369744	27.36160917941782	28.658384468694166	21.83105034951828
20-21	22.82322091879432	26.57390436199733	28.67260207602296	21.93027264318539
22-23	22.551485292781535	26.616711635317618	28.528945741443014	22.30285733045783
24-25	22.40224740087009	26.726978736500705	28.609082990668917	22.26169087196029
26-27	22.34995256467648	26.862484788595175	28.594615686944604	22.19294695978374
28-29	22.180853626579207	26.80712025297862	28.64255488665434	22.369471233787827
30-31	22.56706705873851	26.613284715766046	28.666252282108978	22.15339594338647
32-33	22.239533851115556	26.917696279623364	28.536253089339493	22.30651677992159
34-35	22.352802363782175	26.91131938901432	28.644780507332047	22.09109773987146
36-37	22.441502520304144	26.801784436211417	28.468883994235856	22.287829049248586
38-39	22.207398893861935	26.86249527138206	28.657415267361724	22.27269056739428
40-41	22.48013561297297	26.7246697096029	28.542109688687823	22.253084988736312
42-43	22.43891817239635	26.773717385380785	28.41714471666778	22.37021972555509
44-45	22.397306047523507	26.917452119279705	28.351217564304743	22.334024268892044
46-47	22.576099384458765	26.847393777736027	28.00535422975306	22.571152608052145
48-49	22.467188403844638	26.920449748788812	28.02007796556918	22.592283881797375
50-51	22.359830641716858	27.123719444633583	28.014397930065428	22.50205198358413
52-53	22.583575300641783	27.051797619789397	27.96394167860926	22.40068540095956
54-55	22.48598969853467	26.96922344083294	27.900023572659176	22.644763287973213
56-57	22.583964951913256	27.177651499691617	27.74669263438998	22.491690914005147
58-59	22.456477953321407	27.281125315245582	27.90258740547884	22.359809325954174
60-61	22.60276375703317	27.365502355299085	27.752139447714256	22.279594439953488
62-63	22.451272511983124	27.522583080399386	27.686979374033722	22.339165033583768
64-65	22.43070543305778	27.46107661408964	27.68030045032243	22.427917502530146
66-67	22.490693449526	27.436905115557842	27.57969117743088	22.49271025748528
68-69	22.459589080787097	27.365237373415237	27.68292341706965	22.492250128728024
70-71	22.486710170087733	27.104893850380762	27.739438672660356	22.66895730687115
72-73	22.596573854142267	27.01628280727909	27.715507425200297	22.671635913378342
74-75	22.468850269868078	27.13059354205204	27.704406244202538	22.696149943877337
76-77	22.631190516448783	27.091311546572506	27.58603783774603	22.691460099232682
78-79	22.581055830532744	27.109191578170634	27.54631754311439	22.763435048182235
80-81	22.576880858257297	27.172483213158838	27.535558891408556	22.71507703717531
82-83	22.651779201579917	27.130305309188046	27.591606745736012	22.626308743496022
84-85	22.727005138049993	27.12469471179682	27.506990946620785	22.641309203532405
86-87	22.588546019599306	27.15452729919685	27.577355537519892	22.679571143683948
88-89	22.665820430677613	27.155075260674018	27.488785026789813	22.690319281858557
90-91	22.685224062388116	27.182724530990715	27.516791654227994	22.61525975239317
92-93	22.687693304428553	27.190423385492924	27.493176184857404	22.628707125221123
94-95	22.641989492148422	27.23442500836078	27.497596065959417	22.625989433531384
96-97	22.73330373372496	27.320886318529737	27.517359411347275	22.42845053639803
98-99	23.805864590555192	29.255722830193694	27.72454358938351	19.213868989867606
100	22.682719818916446	30.12298614437501	21.66795752749915	25.52633650920939
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1641.0
1	1542.0
2	2068.0
3	2951.5
4	3652.5
5	4917.5
6	6221.0
7	8077.0
8	10562.5
9	11987.0
10	11891.0
11	11580.0
12	12507.5
13	14860.5
14	19401.0
15	24702.5
16	28598.0
17	30632.5
18	30177.0
19	27751.0
20	25065.5
21	23190.0
22	23217.5
23	26186.0
24	32476.0
25	42321.5
26	55498.0
27	70501.5
28	85655.0
29	102184.5
30	118889.0
31	136011.0
32	154956.5
33	173934.0
34	193653.0
35	212466.0
36	232069.5
37	252737.0
38	269235.5
39	282282.0
40	296468.0
41	309799.0
42	321264.0
43	339410.0
44	353452.5
45	360313.0
46	363688.5
47	362082.5
48	360870.5
49	358179.0
50	354266.0
51	346385.5
52	333716.0
53	319784.5
54	304668.5
55	285492.5
56	263622.0
57	239848.0
58	213742.5
59	186808.0
60	158795.5
61	130294.5
62	103762.5
63	80823.5
64	61225.5
65	45089.0
66	33155.5
67	25412.5
68	19349.0
69	14087.0
70	10235.0
71	7363.5
72	5114.5
73	3385.5
74	2220.5
75	1433.5
76	912.5
77	533.5
78	316.0
79	195.0
80	127.5
81	89.5
82	62.5
83	46.5
84	33.5
85	23.0
86	18.0
87	10.0
88	4.0
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.00453370705487101
6	3.8759671712627805E-4
7	1.1745355064432668E-5
8	0.0
9	0.0
10-11	0.0
12-13	5.872677532216334E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	4.7117135141543704E-5
34-35	1.7707440152836456E-4
36-37	1.8950064566423114E-4
38-39	1.5471936614570077E-4
40-41	1.2563388276420388E-4
42-43	1.864677244519518E-4
44-45	1.4529421988718268E-4
46-47	4.269718873169953E-5
48-49	3.011525970324423E-4
50-51	5.137670059200196E-4
52-53	3.676227995672519E-4
54-55	0.0022142002242928373
56-57	0.003411590588407137
58-59	0.0020177346784558386
60-61	0.003566126798256318
62-63	0.002107020346189906
64-65	0.0028529876936571243
66-67	7.423374840947625E-4
68-69	8.221552268850063E-4
70-71	4.2171164321749117E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	264.0
22-23	587.0
24-25	693.0
26-27	1722.0
28-29	4225.0
30-31	13118.0
32-33	17012.0
34-35	24132.0
36-37	38619.0
38-39	45018.0
40-41	44384.0
42-43	50543.0
44-45	60380.0
46-47	62763.0
48-49	59267.0
50-51	53894.0
52-53	52308.0
54-55	56155.0
56-57	58736.0
58-59	59458.0
60-61	59936.0
62-63	59381.0
64-65	63154.0
66-67	68771.0
68-69	70489.0
70-71	78512.0
72-73	62013.0
74-75	61094.0
76-77	63793.0
78-79	66068.0
80-81	65074.0
82-83	61614.0
84-85	61116.0
86-87	63102.0
88-89	66236.0
90-91	66970.0
92-93	67490.0
94-95	80571.0
96-97	267271.0
98-99	591808.0
100-101	5766178.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.95588838891881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8739622678575	76.35322307498001
2	7.040075959131531	11.961918148669778
3	1.6701870208859801	4.256766664050304
4	0.6290549474758894	2.137676876330354
5	0.3127395329084589	1.328453242628682
6	0.16039786367712422	0.817604580262487
7	0.08648084292379485	0.5142939787449474
8	0.053439183270421305	0.36319786316135116
9	0.03744490419163782	0.28630485911146863
>10	0.13285974309345608	1.7053945012223528
>50	0.0027206805559700786	0.1574042423142212
>100	5.910519286041298E-4	0.0819046992148132
>500	4.600209968822307E-5	0.035857269309170464
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.349071012886534E-5
2	0.0	2.349071012886534E-5	0.0	2.349071012886534E-5	2.349071012886534E-5
3	0.0	2.349071012886534E-5	0.0	2.349071012886534E-5	2.349071012886534E-5
4	0.0	5.8726775322163343E-5	0.0	4.698142025773068E-5	2.349071012886534E-5
5	0.0	7.047213038659601E-5	0.0	4.698142025773068E-5	2.349071012886534E-5
6	3.5236065193298005E-5	7.047213038659601E-5	0.0	4.698142025773068E-5	2.349071012886534E-5
7	3.5236065193298005E-5	7.047213038659601E-5	0.0	4.698142025773068E-5	2.349071012886534E-5
8	3.5236065193298005E-5	8.221748545102868E-5	0.0	4.698142025773068E-5	2.349071012886534E-5
9	3.5236065193298005E-5	1.2919890570875937E-4	0.0	7.047213038659601E-5	4.698142025773068E-5
10-11	3.5236065193298005E-5	1.2919890570875937E-4	0.0	9.396284051546135E-5	4.698142025773068E-5
12-13	3.5236065193298005E-5	1.4094426077319202E-4	0.0	1.1745355064432669E-4	5.8726775322163343E-5
14-15	4.698142025773068E-5	1.6443497090205735E-4	0.0	1.1745355064432669E-4	9.983551804767768E-5
16-17	4.698142025773068E-5	1.7618032596649003E-4	0.0	1.2919890570875937E-4	1.2919890570875937E-4
18-19	4.698142025773068E-5	1.9379835856313905E-4	0.0	1.4681693830540836E-4	1.350715832409757E-4
20-21	4.698142025773068E-5	2.231617462242207E-4	0.0	1.526896158376247E-4	1.4094426077319202E-4
22-23	5.285409778994701E-5	2.64270488949735E-4	0.0	1.703076484342737E-4	1.4681693830540836E-4
24-25	5.8726775322163343E-5	2.760158440141677E-4	0.0	1.7618032596649003E-4	1.58562293369841E-4
26-27	5.8726775322163343E-5	2.8188852154638404E-4	0.0	2.1141639115978804E-4	1.6443497090205735E-4
28-29	5.8726775322163343E-5	2.8188852154638404E-4	0.0	4.5219616998065773E-4	1.6443497090205735E-4
30-31	5.8726775322163343E-5	3.406152968685474E-4	0.0	0.0013448431548775406	1.703076484342737E-4
32-33	5.8726775322163343E-5	3.406152968685474E-4	0.0	0.0030596649942847103	1.879256810309227E-4
34-35	5.8726775322163343E-5	4.5219616998065773E-4	0.0	0.005649515785992114	1.9967103609535536E-4
36-37	5.8726775322163343E-5	5.755223981572008E-4	0.0	0.009349302631288404	1.9967103609535536E-4
38-39	5.8726775322163343E-5	5.755223981572008E-4	0.0	0.015128017322989278	2.231617462242207E-4
40-41	5.8726775322163343E-5	6.342491734793642E-4	0.0	0.02119449321376875	2.3490710128865337E-4
42-43	5.8726775322163343E-5	6.577398836082294E-4	0.0	0.026661955996262157	2.3490710128865337E-4
44-45	5.8726775322163343E-5	7.810661117847725E-4	0.0	0.03366218761466403	2.4665245635308605E-4
46-47	5.8726775322163343E-5	8.691562747680175E-4	0.0	0.041020652562531096	2.5839781141751873E-4
48-49	5.8726775322163343E-5	8.809016298324502E-4	0.0	0.04831451805754378	2.7014316648195136E-4
50-51	8.221748545102868E-5	9.513737602190462E-4	0.0	0.05541458519399334	2.760158440141677E-4
52-53	8.221748545102868E-5	9.631191152834789E-4	0.0	0.06247941626524958	2.877611990786004E-4
54-55	8.221748545102868E-5	9.689917928156952E-4	0.0	0.06928584952508832	2.936338766108167E-4
56-57	8.221748545102868E-5	9.748644703479115E-4	0.0	0.07614513688271698	3.053792316752494E-4
58-59	8.221748545102868E-5	9.748644703479115E-4	0.0	0.08242890184218847	3.229972642718984E-4
60-61	8.221748545102868E-5	9.866098254123442E-4	0.0	0.0887713935769821	3.288699418041147E-4
62-63	8.221748545102868E-5	0.0010394639232022911	0.0	0.09482025143516493	3.288699418041147E-4
64-65	8.221748545102868E-5	0.0011334267637177525	0.0	0.10089847268100885	3.288699418041147E-4
66-67	8.221748545102868E-5	0.001156917473846618	0.0	0.10660084256479091	3.288699418041147E-4
68-69	8.221748545102868E-5	0.0011686628289110505	0.0	0.11282000807140799	3.288699418041147E-4
70-71	9.396284051546135E-5	0.0011921535390399158	0.0	0.11771782113327642	3.288699418041147E-4
72-73	1.0570819557989402E-4	0.0012156442491687812	0.0	0.12134713584818611	3.288699418041147E-4
74-75	1.0570819557989402E-4	0.0012508803143620791	0.0	0.1241836390962466	3.288699418041147E-4
76-77	1.0570819557989402E-4	0.0012978617346198098	0.0	0.12693205218132386	3.288699418041147E-4
78-79	1.0570819557989402E-4	0.0013037344121520262	0.0	0.12908145215811503	3.288699418041147E-4
80-81	1.0570819557989402E-4	0.0013154797672164588	0.0	0.12979204613951323	3.288699418041147E-4
82-83	1.0570819557989402E-4	0.0013213524447486752	0.0	0.13034407782754154	3.5236065193298006E-4
84-85	1.0570819557989402E-4	0.0013389704773453242	0.0	0.1305789849288302	3.5236065193298006E-4
86-87	1.0570819557989402E-4	0.0013389704773453242	0.0	0.13070818383453894	3.6410600699741274E-4
88	1.0570819557989402E-4	0.0013742065425386223	0.0	0.13071405651207116	3.6410600699741274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10555	0.0	50.2271	1
GTATCAA	18880	0.0	37.241753	1
TCAACGC	22620	0.0	30.64557	4
ATCAACG	22830	0.0	30.306997	3
CAACGCA	23280	0.0	29.721165	5
TATCAAC	23870	0.0	29.257612	2
AACGCAG	24130	0.0	28.797657	6
CTTATAC	6325	0.0	26.256971	1
ACGCAGA	27885	0.0	24.88882	7
CGCAGAG	28600	0.0	24.236439	8
GTGGTAT	5240	0.0	23.626265	1
TGGTATC	5140	0.0	22.827074	2
TATACAC	9380	0.0	21.108349	3
GCAGAGT	33775	0.0	20.561247	9
GTACATG	23985	0.0	20.035027	1
TACATGG	24065	0.0	19.448599	2
GAGTACT	19950	0.0	19.329184	12-13
CAGAGTA	32620	0.0	18.618196	10-11
ACATGGG	24455	0.0	18.450514	3
AGAGTAC	30270	0.0	18.21115	10-11
>>END_MODULE