FastQCFastQC Report
Fri 27 May 2016
SRR2138745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7004676
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111000.054.2528461
GTATCAA209650.038.509381
CTTATAC61800.032.0904121
TCAACGC251550.031.688074
ATCAACG252100.031.5512683
CAACGCA257000.030.9828955
TATCAAC258500.030.93512
AACGCAG265950.030.0509836
ACGCAGA302700.026.3599347
CGCAGAG306450.026.0234538
TATACAC87300.025.7458483
GTGGTAT45300.024.855161
TGGTATC45150.023.2374322
GCAGAGT350400.022.6985269
TTATACA95100.022.019722
GTACATG246250.021.1297511
GAGTACT218050.021.0836412-13
TACATGG239950.021.0091042
ACATCTC102650.020.3157488
CAGAGTA340400.020.30216610-11
Produced by FastQC (version 0.11.5)