##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7004676
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20343053697273	33.0	32.0	34.0	31.0	34.0
2	32.00037717661745	33.0	32.0	33.0	30.0	34.0
3	32.045792553431454	33.0	33.0	33.0	29.0	34.0
4	32.47299975045241	33.0	33.0	34.0	31.0	34.0
5	32.80853047307256	33.0	33.0	34.0	31.0	34.0
6	36.82773778544504	38.0	37.0	38.0	35.0	38.0
7	37.112746685214276	38.0	38.0	38.0	36.0	38.0
8	37.156686761814534	38.0	38.0	38.0	36.0	38.0
9	37.238742377234864	38.0	38.0	38.0	36.0	38.0
10-11	37.283252430233745	38.0	38.0	38.0	37.0	38.0
12-13	37.317831331527685	38.0	38.0	38.0	37.0	38.0
14-15	37.34318446706172	38.0	38.0	38.0	37.0	38.0
16-17	37.34061239948856	38.0	38.0	38.0	37.0	38.0
18-19	37.216189228452535	38.0	38.0	38.0	36.5	38.0
20-21	37.33165771534752	38.0	38.0	38.0	37.0	38.0
22-23	37.351787764954324	38.0	38.0	38.0	37.0	38.0
24-25	37.35656112524194	38.0	38.0	38.0	37.0	38.0
26-27	37.324684464325045	38.0	38.0	38.0	37.0	38.0
28-29	37.29818307945908	38.0	38.0	38.0	37.0	38.0
30-31	37.300241857417234	38.0	38.0	38.0	37.0	38.0
32-33	37.27950500766555	38.0	38.0	38.0	37.0	38.0
34-35	37.26002502140216	38.0	38.0	38.0	37.0	38.0
36-37	37.230984911345296	38.0	38.0	38.0	37.0	38.0
38-39	37.17980248399789	38.0	38.0	38.0	36.5	38.0
40-41	37.192509109414786	38.0	38.0	38.0	37.0	38.0
42-43	37.16556180888246	38.0	38.0	38.0	36.5	38.0
44-45	37.12723391155867	38.0	38.0	38.0	36.0	38.0
46-47	37.07904014098956	38.0	38.0	38.0	36.0	38.0
48-49	37.03936051616367	38.0	38.0	38.0	36.0	38.0
50-51	37.01363016674879	38.0	38.0	38.0	36.0	38.0
52-53	36.994645992271366	38.0	38.0	38.0	36.0	38.0
54-55	36.98048768185191	38.0	38.0	38.0	36.0	38.0
56-57	36.97284249608077	38.0	38.0	38.0	36.0	38.0
58-59	36.96330614420151	38.0	38.0	38.0	36.0	38.0
60-61	36.96331010362681	38.0	38.0	38.0	36.0	38.0
62-63	36.95890527152227	38.0	38.0	38.0	36.0	38.0
64-65	36.96439446248736	38.0	38.0	38.0	36.0	38.0
66-67	36.95814768474892	38.0	38.0	38.0	36.0	38.0
68-69	36.96035083351967	38.0	38.0	38.0	36.0	38.0
70-71	36.954235591985935	38.0	38.0	38.0	36.0	38.0
72-73	36.92610334945296	38.0	38.0	38.0	36.0	38.0
74-75	36.95066054846098	38.0	38.0	38.0	36.0	38.0
76-77	36.94310118246262	38.0	38.0	38.0	36.0	38.0
78-79	36.93444463532326	38.0	38.0	38.0	36.0	38.0
80-81	36.91379991743959	38.0	38.0	38.0	35.5	38.0
82-83	36.90516640309639	38.0	38.0	38.0	35.5	38.0
84-85	36.890640090016575	38.0	38.0	38.0	35.0	38.0
86-87	36.855597955422525	38.0	38.0	38.0	35.0	38.0
88-89	36.84019621903673	38.0	38.0	38.0	35.0	38.0
90-91	36.848526168096896	38.0	38.0	38.0	35.0	38.0
92-93	36.817019703476106	38.0	38.0	38.0	35.0	38.0
94-95	36.758924189217424	38.0	38.0	38.0	35.0	38.0
96-97	36.779103842961476	38.0	38.0	38.0	35.0	38.0
98-99	36.80983566739237	38.0	38.0	38.0	35.0	38.0
100	35.22942581298267	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	12.0
19	46.0
20	73.0
21	233.0
22	620.0
23	1615.0
24	3890.0
25	7528.0
26	13623.0
27	21792.0
28	33236.0
29	48693.0
30	66118.0
31	86623.0
32	110725.0
33	145749.0
34	205783.0
35	337357.0
36	824804.0
37	5096150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50650222794031	19.822287283523178	14.007114675967882	24.664095812568632
2	14.56621262710795	20.192825478294786	42.94841046181151	22.292551432785757
3	18.316907163157868	25.985555934350142	30.073810694456103	25.62372620803589
4	12.428926048827954	17.902084264853933	38.92828162216211	30.740708064156003
5	13.40770844939276	37.286192480805404	35.34331841776368	13.962780652038159
6	29.562367980593468	37.684099081581785	18.828829754854347	13.924703182970402
7	26.205380520098288	32.817991867147036	22.887154238111798	18.089473374642882
8	24.029691023539133	36.93876776027899	21.20496365570656	17.826577560475318
9	25.40374458433195	16.972233976275277	21.361616154694378	36.26240528469839
10-11	24.22301188520354	26.87089310055169	28.540499232227155	20.36559578201761
12-13	25.15364915905098	24.337137387734806	28.84883817958448	21.660375273629732
14-15	22.254633120684847	25.550903582379426	26.732378980092435	25.4620843168433
16-17	21.618030584141223	28.803659155684002	27.38771928922908	22.190590970945696
18-19	21.580912521863965	27.493156000363182	29.505932893969682	21.419998583803164
20-21	22.38697203057922	26.64656833216017	29.523808096168203	21.442651541092406
22-23	22.089277072766585	26.79256618562321	29.36842674096131	21.7497300006489
24-25	21.91332215571256	26.923768602546343	29.538239563994257	21.624669677746837
26-27	21.83040540545365	27.09543475732344	29.508966213130055	21.56519362409286
28-29	21.604776199228098	26.989078304057294	29.687918280984256	21.71822721573035
30-31	21.99824551633342	27.104134703625444	29.37089442993972	21.526725350101415
32-33	22.02885544416599	26.961204010024343	29.36882297033164	21.64111757547803
34-35	21.96653266444233	26.97019027320198	29.467316027449154	21.59596103490654
36-37	21.743328480235693	27.159946167877493	29.369050940372055	21.727674411514762
38-39	21.653199247645123	27.008908122951112	29.58186497734702	21.75602765205674
40-41	21.915211423032318	26.84438265431363	29.489163285169024	21.751242637485028
42-43	21.956923915952405	26.868364184134265	29.316280227825136	21.85843167208819
44-45	21.87261737653829	27.12296671400401	29.175956888197003	21.8284590212607
46-47	22.095648653261136	26.97644325842898	28.762361068511886	22.165547019797998
48-49	21.99648411605578	27.121429100404544	28.685958319961998	22.196128463577683
50-51	21.88443092129152	27.315063164525544	28.692872648997408	22.107633265185527
52-53	22.06772992384719	27.275412523059405	28.72010917728754	21.936748375805866
54-55	22.013963064324468	27.237150610426653	28.517176495058997	22.231709830189878
56-57	22.17773427791751	27.408360340388306	28.28663846666203	22.12726691503216
58-59	21.92317145362155	27.63942264237779	28.5886888521882	21.848717051812464
60-61	22.13730918067271	27.852452802822913	28.218920075691805	21.791317940812576
62-63	21.921754440841536	28.035098568823376	28.19528938918665	21.84785760114844
64-65	21.86677734003052	28.008457994878277	28.15731259788044	21.967452067210765
66-67	21.990509107553983	27.959774530457032	28.053422697304104	21.99629366468488
68-69	21.99873169242795	27.82969087852646	28.176839437558975	21.99473799148661
70-71	22.004901048390387	27.498466348755446	28.29433098134768	22.202301621506486
72-73	22.153219470956763	27.368828251555	28.265224930869433	22.212727346618806
74-75	22.0151093059161	27.50354319064413	28.217225158056525	22.264122345383246
76-77	22.145261530282873	27.430132477138148	28.12935968670075	22.29524630587823
78-79	22.129712041353066	27.411110516613412	28.114893028878672	22.34428441315485
80-81	22.147341235413723	27.4944329057062	28.042836888896883	22.31538896998319
82-83	22.218556870572755	27.43872441762964	28.169845905832595	22.172872805965007
84-85	22.314003643877157	27.44403781714101	28.038023112454795	22.20393542652704
86-87	22.1778709424539	27.507464374362222	28.0692592463647	22.245405436819173
88-89	22.248838205512538	27.490526633813122	27.97891250637034	22.281722654304005
90-91	22.292713464524596	27.470536362659857	28.01952147162485	22.21722870119069
92-93	22.3186140095123	27.471588153738157	27.999803421944574	22.209994414804964
94-95	22.224693103404217	27.58082130211492	27.97966662081914	22.21481897366172
96-97	22.263875913862556	27.673591060062915	28.03267998369608	22.02985304237845
98-99	23.318424109853385	29.58567889882723	28.22935532984982	18.866541661469572
100	22.221413784592567	30.472567859564798	22.157847350371085	25.148171005471546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1924.0
1	1777.0
2	2476.0
3	3578.5
4	4499.5
5	6224.0
6	7850.5
7	9989.5
8	12683.5
9	14064.5
10	13562.5
11	12946.0
12	14095.5
13	17205.0
14	23032.5
15	29541.5
16	34146.5
17	36317.0
18	35377.5
19	32210.0
20	28638.5
21	25781.5
22	25302.5
23	28152.5
24	34217.5
25	43960.5
26	56960.5
27	70766.5
28	84358.0
29	99358.0
30	114330.0
31	129520.5
32	146249.5
33	161763.0
34	177180.5
35	192686.5
36	208345.5
37	223588.5
38	234775.0
39	243585.0
40	252575.5
41	259262.0
42	266097.5
43	282965.5
44	293986.0
45	295129.5
46	292658.0
47	286982.5
48	284525.0
49	280224.0
50	274926.0
51	267494.0
52	257389.5
53	245807.5
54	233410.0
55	218582.0
56	200829.5
57	181436.5
58	162373.0
59	144093.5
60	122889.0
61	100270.0
62	79003.0
63	61137.5
64	46888.5
65	34991.0
66	25587.0
67	19031.0
68	14193.5
69	10248.5
70	7559.0
71	5621.0
72	3995.5
73	2776.0
74	1897.0
75	1278.0
76	819.5
77	482.0
78	302.0
79	203.5
80	140.0
81	95.5
82	68.0
83	51.5
84	36.5
85	22.0
86	12.0
87	8.0
88	7.0
89	6.0
90	5.5
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.005167976363217942
6	3.426282671746702E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.1380888994722945E-6
14-15	2.8552355597889178E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	7.16228740540409E-5
34-35	1.866856109480484E-4
36-37	2.4504467416661927E-4
38-39	1.95683781097686E-4
40-41	8.021949804909827E-5
42-43	1.9812884186500585E-4
44-45	1.330737212890674E-4
46-47	6.713102940137695E-5
48-49	3.461985219731449E-4
50-51	5.387849261636995E-4
52-53	4.2054610894300473E-4
54-55	0.002064564954949729
56-57	0.0033239940491186916
58-59	0.002161684271600904
60-61	0.0035073455981943806
62-63	0.0022865669054256805
64-65	0.0028042536912696674
66-67	7.058245698181787E-4
68-69	8.446565142780574E-4
70-71	4.893725290316106E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.878996743510744E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	68.0
20-21	286.0
22-23	610.0
24-25	727.0
26-27	1723.0
28-29	4285.0
30-31	12332.0
32-33	16106.0
34-35	22688.0
36-37	36165.0
38-39	43305.0
40-41	41525.0
42-43	47804.0
44-45	58233.0
46-47	60881.0
48-49	56130.0
50-51	50836.0
52-53	47809.0
54-55	51712.0
56-57	53960.0
58-59	54168.0
60-61	54554.0
62-63	52448.0
64-65	57330.0
66-67	64399.0
68-69	67901.0
70-71	76541.0
72-73	58389.0
74-75	54929.0
76-77	57516.0
78-79	59216.0
80-81	58843.0
82-83	55218.0
84-85	54335.0
86-87	56157.0
88-89	59024.0
90-91	59767.0
92-93	59088.0
94-95	67884.0
96-97	211567.0
98-99	467744.0
100-101	4590473.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.10836474029038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27156264342699	77.73136641776183
2	6.942877278219376	11.95679618039977
3	1.6527214067502762	4.269394131196158
4	0.5407245084494318	1.862436127903116
5	0.23832870956287938	1.0261047725561576
6	0.11481216003642383	0.5931772411822186
7	0.07014400940213168	0.42279901621615784
8	0.04214728002186947	0.29033866887474324
9	0.027254509481867077	0.21121571189540822
>10	0.09621258882143079	1.3109549618495375
>50	0.002608244280410276	0.14959489209963758
>100	5.294060957536101E-4	0.10546228931672798
>500	2.5751817179364274E-5	0.018515681334489893
>1k	5.1503634038371655E-5	0.05184390741409706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.427617779894459E-5	0.0	0.0	0.0
3	0.0	1.427617779894459E-5	0.0	0.0	0.0
4	0.0	5.710471119577836E-5	0.0	0.0	0.0
5	0.0	5.710471119577836E-5	0.0	0.0	0.0
6	0.0	5.710471119577836E-5	0.0	0.0	0.0
7	0.0	5.710471119577836E-5	0.0	0.0	0.0
8	0.0	5.710471119577836E-5	0.0	0.0	0.0
9	0.0	1.2848560019050132E-4	0.0	0.0	0.0
10-11	1.427617779894459E-5	1.2848560019050132E-4	0.0	2.855235559788918E-5	0.0
12-13	1.427617779894459E-5	1.2848560019050132E-4	0.0	4.282853339683377E-5	0.0
14-15	1.427617779894459E-5	1.9986648918522427E-4	0.0	5.710471119577836E-5	0.0
16-17	1.427617779894459E-5	1.9986648918522427E-4	0.0	7.138088899472295E-5	0.0
18-19	1.427617779894459E-5	2.0700457808469657E-4	0.0	7.138088899472295E-5	0.0
20-21	1.427617779894459E-5	2.3555693368258575E-4	0.0	7.138088899472295E-5	0.0
22-23	1.427617779894459E-5	2.926616448783641E-4	0.0	7.851897789419525E-5	0.0
24-25	2.1414266698416886E-5	3.14075911576781E-4	0.0	1.1420942239155673E-4	0.0
26-27	2.855235559788918E-5	3.14075911576781E-4	0.0	1.498998668889182E-4	0.0
28-29	2.855235559788918E-5	3.14075911576781E-4	0.0	3.5690444497361477E-4	0.0
30-31	3.569044449736148E-5	3.426282671746702E-4	0.0	0.0011278180461166227	0.0
32-33	5.710471119577836E-5	3.426282671746702E-4	0.0	0.002997997337778364	0.0
34-35	5.710471119577836E-5	3.9973297837044854E-4	0.0	0.005453499919196834	0.0
36-37	5.710471119577836E-5	4.282853339683377E-4	0.0	0.009236687035917152	0.0
38-39	5.710471119577836E-5	4.3542342286781E-4	0.0	0.014697325044013457	0.0
40-41	5.710471119577836E-5	4.425615117672823E-4	0.0	0.02105736225344327	0.0
42-43	5.710471119577836E-5	4.568376895662269E-4	0.0	0.027503056529666753	0.0
44-45	5.710471119577836E-5	4.996662229630606E-4	0.0	0.03464828351803852	0.0
46-47	5.710471119577836E-5	5.282185785609498E-4	0.0	0.04191485801770132	0.0
48-49	7.138088899472295E-5	5.282185785609498E-4	0.0	0.04922426105076095	0.0
50-51	7.138088899472295E-5	5.781852008572559E-4	0.0	0.05729743959606411	0.0
52-53	7.138088899472295E-5	5.853232897567282E-4	0.0	0.06452118556233008	0.0
54-55	7.138088899472295E-5	5.853232897567282E-4	0.0	0.07135233663912506	0.0
56-57	7.138088899472295E-5	5.853232897567282E-4	0.0	0.0763918274021525	0.0
58-59	7.138088899472295E-5	5.924613786562004E-4	0.0	0.08233071736651346	0.0
60-61	7.138088899472295E-5	5.995994675556728E-4	0.0	0.08751296990753034	0.0
62-63	7.138088899472295E-5	5.995994675556728E-4	0.0	0.0924453893370657	0.0
64-65	7.138088899472295E-5	6.28151823153562E-4	0.0	0.09777040365607204	0.0
66-67	7.138088899472295E-5	6.424280009525065E-4	0.0	0.10328814637536411	0.0
68-69	7.138088899472295E-5	6.852565343493404E-4	0.0	0.10824198007159788	7.138088899472295E-6
70-71	7.138088899472295E-5	7.066708010477572E-4	0.0	0.11269614754486859	1.427617779894459E-5
72-73	7.138088899472295E-5	7.138088899472295E-4	0.0	0.11641509186149367	1.427617779894459E-5
74-75	7.138088899472295E-5	7.423612455451187E-4	0.0	0.11929887977688047	1.427617779894459E-5
76-77	7.138088899472295E-5	7.637755122435355E-4	0.0	0.12193283458078574	1.427617779894459E-5
78-79	7.138088899472295E-5	7.709136011430079E-4	0.0	0.12390294711704011	1.427617779894459E-5
80-81	7.138088899472295E-5	7.709136011430079E-4	0.0	0.12460247982918839	1.427617779894459E-5
82-83	7.138088899472295E-5	7.780516900424802E-4	0.0	0.1250878698743525	1.427617779894459E-5
84-85	7.138088899472295E-5	7.851897789419524E-4	0.0	0.12528773636353774	1.427617779894459E-5
86-87	7.138088899472295E-5	7.851897789419524E-4	0.0	0.12540194578592928	1.427617779894459E-5
88	7.138088899472295E-5	9.279515569313983E-4	0.0	0.12540194578592928	1.427617779894459E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11100	0.0	54.252846	1
GTATCAA	20965	0.0	38.50938	1
CTTATAC	6180	0.0	32.090412	1
TCAACGC	25155	0.0	31.68807	4
ATCAACG	25210	0.0	31.551268	3
CAACGCA	25700	0.0	30.982895	5
TATCAAC	25850	0.0	30.9351	2
AACGCAG	26595	0.0	30.050983	6
ACGCAGA	30270	0.0	26.359934	7
CGCAGAG	30645	0.0	26.023453	8
TATACAC	8730	0.0	25.745848	3
GTGGTAT	4530	0.0	24.85516	1
TGGTATC	4515	0.0	23.237432	2
GCAGAGT	35040	0.0	22.698526	9
TTATACA	9510	0.0	22.01972	2
GTACATG	24625	0.0	21.129751	1
GAGTACT	21805	0.0	21.08364	12-13
TACATGG	23995	0.0	21.009104	2
ACATCTC	10265	0.0	20.315748	8
CAGAGTA	34040	0.0	20.302166	10-11
>>END_MODULE