FastQCFastQC Report
Fri 27 May 2016
SRR2138744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7172118
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116750.052.3829151
GTATCAA216150.037.270311
TCAACGC266400.030.3043274
ATCAACG267250.030.2079453
CAACGCA269950.029.921645
TATCAAC275300.029.6661622
AACGCAG278500.029.0329236
TGGTATC51700.025.9564932
GTGGTAT53800.025.3405081
ACGCAGA319650.025.308757
CGCAGAG319700.025.2914268
GCAGAGT365200.022.1286839
GAGTACT229300.021.097812-13
TACATGG232900.019.9098132
CAGAGTA361950.019.6471510-11
GTACATG239950.019.4851381
ACATGGG234000.019.195253
AGAGTAC342500.018.73525210-11
GTACTTT258200.018.68708614-15
CATGGGG157950.017.9661644
Produced by FastQC (version 0.11.5)