##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7172118
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.87418751336774	18.0	18.0	31.0	18.0	33.0
2	31.22669147384357	32.0	31.0	33.0	27.0	33.0
3	31.789654604121125	33.0	31.0	33.0	29.0	33.0
4	32.302763005293556	33.0	33.0	33.0	31.0	34.0
5	32.56705759721187	33.0	33.0	34.0	31.0	34.0
6	36.962139078024094	38.0	37.0	38.0	35.0	38.0
7	37.1346763954525	38.0	38.0	38.0	36.0	38.0
8	37.24388764936662	38.0	38.0	38.0	36.0	38.0
9	37.34781636331137	38.0	38.0	38.0	37.0	38.0
10-11	37.37275139644942	38.0	38.0	38.0	37.0	38.0
12-13	37.397270513396464	38.0	38.0	38.0	37.0	38.0
14-15	37.38952712434458	38.0	38.0	38.0	37.0	38.0
16-17	37.39505742933956	38.0	38.0	38.0	37.0	38.0
18-19	37.2784971608108	38.0	38.0	38.0	37.0	38.0
20-21	37.385736312335126	38.0	38.0	38.0	37.0	38.0
22-23	37.39926415816089	38.0	38.0	38.0	37.0	38.0
24-25	37.40636865354951	38.0	38.0	38.0	37.0	38.0
26-27	37.37554428545346	38.0	38.0	38.0	37.0	38.0
28-29	37.34997580305745	38.0	38.0	38.0	37.0	38.0
30-31	37.35248910097756	38.0	38.0	38.0	37.0	38.0
32-33	37.335044989187786	38.0	38.0	38.0	37.0	38.0
34-35	37.31182527047899	38.0	38.0	38.0	37.0	38.0
36-37	37.27881281151393	38.0	38.0	38.0	37.0	38.0
38-39	37.24155456825423	38.0	38.0	38.0	37.0	38.0
40-41	37.24376487502558	38.0	38.0	38.0	37.0	38.0
42-43	37.21525223628761	38.0	38.0	38.0	37.0	38.0
44-45	37.173120137581016	38.0	38.0	38.0	37.0	38.0
46-47	37.123494668803396	38.0	38.0	38.0	36.5	38.0
48-49	37.07784825109568	38.0	38.0	38.0	36.0	38.0
50-51	37.04511626423546	38.0	38.0	38.0	36.0	38.0
52-53	37.025820462767236	38.0	38.0	38.0	36.0	38.0
54-55	37.01299473141046	38.0	38.0	38.0	36.0	38.0
56-57	37.00098525447518	38.0	38.0	38.0	36.0	38.0
58-59	36.991255408497594	38.0	38.0	38.0	36.0	38.0
60-61	36.9929504280345	38.0	38.0	38.0	36.0	38.0
62-63	36.991791737614534	38.0	38.0	38.0	36.0	38.0
64-65	37.00016121768841	38.0	38.0	38.0	36.0	38.0
66-67	36.995146357325126	38.0	38.0	38.0	36.0	38.0
68-69	37.000582724176866	38.0	38.0	38.0	36.0	38.0
70-71	36.997306286143655	38.0	38.0	38.0	36.0	38.0
72-73	36.975369308585954	38.0	38.0	38.0	36.0	38.0
74-75	37.0009625513297	38.0	38.0	38.0	36.0	38.0
76-77	36.994976373037495	38.0	38.0	38.0	36.0	38.0
78-79	36.98819415173043	38.0	38.0	38.0	36.0	38.0
80-81	36.970745932352585	38.0	38.0	38.0	36.0	38.0
82-83	36.96188645254983	38.0	38.0	38.0	36.0	38.0
84-85	36.951105486935234	38.0	38.0	38.0	36.0	38.0
86-87	36.92066800035536	38.0	38.0	38.0	36.0	38.0
88-89	36.9073062002507	38.0	38.0	38.0	35.0	38.0
90-91	36.916209043609655	38.0	38.0	38.0	35.0	38.0
92-93	36.89029946945381	38.0	38.0	38.0	35.0	38.0
94-95	36.83212935204084	38.0	38.0	38.0	35.0	38.0
96-97	36.84610849254341	38.0	38.0	38.0	35.0	38.0
98-99	36.877566330389854	38.0	38.0	38.0	35.0	38.0
100	35.290433853220776	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	28.0
20	88.0
21	205.0
22	611.0
23	1594.0
24	3578.0
25	7370.0
26	13341.0
27	21636.0
28	33931.0
29	49681.0
30	65925.0
31	84072.0
32	107012.0
33	140930.0
34	203019.0
35	358476.0
36	1095695.0
37	4984921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.749831500262545	20.1523176277914	16.519848111812998	22.57800276013306
2	14.128560071097548	21.377436902181476	42.5932479080796	21.90075511864138
3	18.136943647608696	26.97048486932312	30.60353998637502	24.289031496693166
4	12.116727583121193	18.81119356931941	39.685362120366676	29.38671672719272
5	12.973723350959112	37.85008423870948	35.86837260685167	13.30781980347973
6	28.466778962972754	38.71849474428469	19.372716236269532	13.442010056473022
7	25.41893482511024	33.265375723043036	23.73837128725434	17.577318164592384
8	23.619382726274164	37.09099599309437	21.687247755823314	17.602373524808154
9	25.315116120510005	17.185258245890545	21.962299002888685	35.53732663071076
10-11	23.684007987598644	27.316345046191376	29.11077313563441	19.88887383057557
12-13	24.708964960487613	24.906549565034314	29.409452647701322	20.97503282677675
14-15	21.852221467369993	26.09060713241243	27.45021257669022	24.60695882352736
16-17	20.949334631694573	29.270502799870275	28.13498048972424	21.64518207871092
18-19	20.92522738750255	28.045522954307224	30.298609141678927	20.7306405165113
20-21	21.854656307565552	27.131095408503626	30.32491067851285	20.68933760541797
22-23	21.551989615595627	27.174602439774475	30.141928698971704	21.13147924565819
24-25	21.299371635942943	27.284504764642037	30.303591920782537	21.112531678632486
26-27	21.237887207171095	27.47470850740183	30.193548756020007	21.093855529407072
28-29	21.110742948425056	27.502345583849824	30.238395027711025	21.148516440014088
30-31	21.464382598141444	27.353386005728233	30.19619282503509	20.986038571095236
32-33	21.303135686202637	27.447203141946876	30.15084716655725	21.09881400529324
34-35	21.369734130993418	27.520268709911488	30.134805881900405	20.97519127719469
36-37	21.321874223711678	27.43734207185119	30.071223012619186	21.169560691817946
38-39	21.149244412501602	27.50546732934065	30.201161338115984	21.144126920041764
40-41	21.4538175840559	27.3949879424923	30.010314455214377	21.14088001823742
42-43	21.344671170120233	27.452609985367182	29.889807666049172	21.312911178463416
44-45	21.395613096484052	27.627627041982834	29.663770118270065	21.312989743263046
46-47	21.47089277182072	27.582760132560537	29.31327066848458	21.633076427134164
48-49	21.488433579177823	27.713665362171685	29.25153460334516	21.54636645530533
50-51	21.444224506063946	27.86731611247214	29.06283825044824	21.62562113101567
52-53	21.558139818113364	27.90889992558932	28.96811084957663	21.56484940672069
54-55	21.55788136190165	27.838672282557326	28.94594133296729	21.657505022573737
56-57	21.654255805162933	27.875443251823906	28.831013239642417	21.63928770337074
58-59	21.53641637895218	28.028141935834032	28.997637511471314	21.437804173742474
60-61	21.665627373148823	28.073120523948862	28.85543140651219	21.405820696390126
62-63	21.501837868899205	28.200108956816223	28.824029766604177	21.4740234076804
64-65	21.450086706199258	28.11264279868169	28.820960322114313	21.616310173004738
66-67	21.49472572379131	28.1482296115055	28.738513391894642	21.61853127280855
68-69	21.526352440039265	28.031895958990226	28.819602614928485	21.62214898604202
70-71	21.545651982448913	27.826261257323075	28.84646076519322	21.781625995034787
72-73	21.631186000839346	27.81410293646375	28.78721325406341	21.767497808633497
74-75	21.561457173837095	27.879625672108137	28.75748002438169	21.80143712967308
76-77	21.742991454703546	27.86245072442892	28.564849174175215	21.829708646692318
78-79	21.67947580628914	27.884339664201207	28.557956921804088	21.878227607705565
80-81	21.69074353081094	27.88408030042526	28.563203740084862	21.861972428678936
82-83	21.782438711374276	27.874166786964334	28.607475646683834	21.73591885497756
84-85	21.874881597925846	27.935830790265832	28.421030595874498	21.768257015933827
86-87	21.7123029622369	27.988187146446208	28.48534517646909	21.814164714847802
88-89	21.816457835301634	27.936333127722907	28.43427216996658	21.81293686700888
90-91	21.84587208729793	27.990902778867987	28.391941197206716	21.771283936627366
92-93	21.92410902677338	28.049615333287647	28.346356937526373	21.6799187024126
94-95	21.791540772551365	28.119699260131487	28.34539913901601	21.74336082830114
96-97	21.848702609597538	28.177504491322946	28.438751319615506	21.535041579464004
98-99	22.91200734792593	30.022456658967943	28.717594273701845	18.347941719404275
100	21.802660606641926	30.997237362329262	22.735232642340137	24.46486938868868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1671.0
1	1661.0
2	2420.0
3	3414.0
4	4501.5
5	6386.0
6	7999.5
7	10278.0
8	12822.5
9	13848.0
10	13688.0
11	13535.0
12	14866.0
13	18378.5
14	25403.0
15	33576.5
16	38898.0
17	41067.0
18	39898.5
19	36020.5
20	31594.0
21	28330.0
22	27852.0
23	31584.0
24	39435.5
25	50542.5
26	64856.0
27	81324.5
28	98115.0
29	116322.0
30	134928.0
31	153003.5
32	172675.5
33	191463.0
34	208652.0
35	224542.0
36	240530.5
37	254248.0
38	263250.0
39	270356.5
40	277481.5
41	282785.5
42	284979.0
43	288305.0
44	295839.5
45	303683.0
46	301822.0
47	297227.0
48	293131.5
49	283772.0
50	273104.0
51	260384.5
52	245069.5
53	228646.5
54	211031.0
55	192660.5
56	174155.0
57	155206.5
58	136444.0
59	117569.0
60	97370.0
61	78242.5
62	61565.0
63	46946.5
64	35197.5
65	26024.5
66	18876.0
67	14348.5
68	10996.5
69	7984.0
70	5944.5
71	4455.0
72	3094.5
73	2007.5
74	1302.0
75	813.0
76	495.5
77	291.5
78	185.0
79	120.5
80	77.0
81	46.0
82	29.0
83	20.0
84	15.0
85	11.0
86	9.5
87	8.0
88	5.5
89	3.5
90	3.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0033044632004102553
6	2.928005367452125E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	6.9714413510764875E-6
14-15	6.9714413510764875E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	5.596126305410132E-5
34-35	2.033721914475682E-4
36-37	2.1821375290065911E-4
38-39	2.123436345713274E-4
40-41	1.495568843170378E-4
42-43	2.2219759731287127E-4
44-45	2.0224225993590943E-4
46-47	5.102404530002212E-5
48-49	3.530685926950697E-4
50-51	4.821198796272753E-4
52-53	4.408284885784327E-4
54-55	0.0019490878682675477
56-57	0.0031921832304075393
58-59	0.0018499001703666325
60-61	0.003291608787284985
62-63	0.002005586101443789
64-65	0.00254781164538068
66-67	7.35802104995321E-4
68-69	6.951573580568578E-4
70-71	3.794169378825048E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	348.0
22-23	724.0
24-25	724.0
26-27	1709.0
28-29	4189.0
30-31	12754.0
32-33	16687.0
34-35	23411.0
36-37	36795.0
38-39	43239.0
40-41	43874.0
42-43	50574.0
44-45	61297.0
46-47	63255.0
48-49	58289.0
50-51	50659.0
52-53	47190.0
54-55	49652.0
56-57	52295.0
58-59	54240.0
60-61	54899.0
62-63	53480.0
64-65	56974.0
66-67	61144.0
68-69	63385.0
70-71	67331.0
72-73	56523.0
74-75	56408.0
76-77	58613.0
78-79	60214.0
80-81	60454.0
82-83	56715.0
84-85	56001.0
86-87	57160.0
88-89	60058.0
90-91	60722.0
92-93	60220.0
94-95	69581.0
96-97	213970.0
98-99	472633.0
100-101	4743655.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.6865669439428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.42826036905463	75.73372358424291
2	7.370866517456838	12.4842676153095
3	1.7939918359448699	4.557810291348962
4	0.6685403121942673	2.2646553561345697
5	0.278987345509947	1.1813240256020512
6	0.15198528019350854	0.7722666963360879
7	0.09276230688679774	0.5499004918430354
8	0.051549929065598854	0.3492469215015472
9	0.04121312018533811	0.31411778953899566
>10	0.11929014124399441	1.5427682556371443
>50	0.0020699627273809383	0.12288327507682426
>100	4.0396416077243674E-4	0.07330382658817577
>500	7.891537611476502E-5	0.05373187084012618
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.3942882702152977E-5	4.182864810645893E-5	0.0	1.3942882702152977E-5	0.0
3	1.3942882702152977E-5	4.182864810645893E-5	0.0	1.3942882702152977E-5	0.0
4	1.3942882702152977E-5	1.5337170972368273E-4	0.0	1.3942882702152977E-5	0.0
5	1.3942882702152977E-5	1.5337170972368273E-4	0.0	2.7885765404305954E-5	0.0
6	1.812574751279887E-4	1.5337170972368273E-4	0.0	2.7885765404305954E-5	1.3942882702152977E-5
7	1.9520035783014166E-4	1.5337170972368273E-4	0.0	8.365729621291786E-5	1.3942882702152977E-5
8	2.0914324053229464E-4	1.812574751279887E-4	0.0	9.760017891507083E-5	1.3942882702152977E-5
9	2.0914324053229464E-4	3.2068630214951844E-4	0.0	9.760017891507083E-5	1.3942882702152977E-5
10-11	2.370290059366006E-4	3.3462918485167143E-4	0.0	9.760017891507083E-5	1.3942882702152977E-5
12-13	2.6491477134090656E-4	3.3462918485167143E-4	0.0	9.760017891507083E-5	1.3942882702152977E-5
14-15	2.6491477134090656E-4	4.3920080511781876E-4	0.0	9.760017891507083E-5	1.3942882702152977E-5
16-17	3.485720675538244E-4	4.670865705221247E-4	0.0	1.4640026837260624E-4	1.3942882702152977E-5
18-19	3.485720675538244E-4	4.949723359264307E-4	0.0	1.6731459242583571E-4	1.3942882702152977E-5
20-21	3.485720675538244E-4	5.019437772775071E-4	0.0	1.6731459242583571E-4	1.3942882702152977E-5
22-23	3.485720675538244E-4	5.437724253839661E-4	0.0	1.6731459242583571E-4	2.0914324053229464E-5
24-25	3.834292743092069E-4	5.925725148415014E-4	0.0	2.0217179918121818E-4	2.7885765404305954E-5
26-27	4.043435983624363E-4	6.204582802458074E-4	0.0	2.9977197809628897E-4	2.7885765404305954E-5
28-29	4.3222936376674227E-4	6.274297215968839E-4	0.0	5.85601073490425E-4	2.7885765404305954E-5
30-31	4.4617224646889526E-4	7.250299005119548E-4	0.0	0.001498859890481445	2.7885765404305954E-5
32-33	4.8102945322427767E-4	7.668585486184136E-4	0.0	0.0038552070671452983	2.7885765404305954E-5
34-35	5.228581013307366E-4	8.714301688845609E-4	0.0	0.0068947554962146464	2.7885765404305954E-5
36-37	5.437724253839661E-4	9.969161132039378E-4	0.0	0.011677164263053118	2.7885765404305954E-5
38-39	5.437724253839661E-4	0.0010317733199593203	0.0	0.02453947355578924	2.7885765404305954E-5
40-41	5.856010734904251E-4	0.0011851450296830029	0.0	0.03823138436930346	2.7885765404305954E-5
42-43	6.204582802458074E-4	0.0012269736777894618	0.0	0.046074255889264504	3.485720675538244E-5
44-45	6.483440456501134E-4	0.0013594310634599152	0.0	0.05592490251833558	4.182864810645893E-5
46-47	6.971441351076488E-4	0.0014500598010239096	0.0	0.06681429390871707	4.880008945753542E-5
48-49	7.110870178098018E-4	0.0014640026837260626	0.0	0.07723659872857641	5.577153080861191E-5
50-51	7.250299005119548E-4	0.0016104029520986687	0.0	0.08781227525815945	6.274297215968839E-5
52-53	7.250299005119548E-4	0.0016173743934497452	0.0	0.11994364844527097	6.971441351076489E-5
54-55	7.459442245651843E-4	0.0016243458348008217	0.0	0.16081023764528135	6.971441351076489E-5
56-57	7.877728726716431E-4	0.0016313172761518982	0.0	0.2150271370326032	6.971441351076489E-5
58-59	8.086871967248726E-4	0.0016592030415562042	0.0	0.25049085918552927	8.365729621291786E-5
60-61	8.226300794270256E-4	0.0017219460137158926	0.0	0.26504025728522596	8.365729621291786E-5
62-63	8.296015207781021E-4	0.0017358888964180456	0.0	0.2779513666674196	9.760017891507083E-5
64-65	8.365729621291786E-4	0.0020356608745143345	0.0	0.28442086424121854	9.760017891507083E-5
66-67	8.644587275334845E-4	0.0020496037572164875	0.0	0.2907160757812406	9.760017891507083E-5
68-69	8.644587275334845E-4	0.0020774895226207935	0.0	0.29697643011450736	9.760017891507083E-5
70-71	8.923444929377905E-4	0.002098403846674023	0.0	0.3019261534737716	9.760017891507083E-5
72-73	9.062873756399435E-4	0.0021053752880250995	0.0	0.30585107495442765	1.1154306161722381E-4
74-75	9.760017891507083E-4	0.002126289612078329	0.0	0.3088139375286352	1.1154306161722381E-4
76-77	0.0010178304372571673	0.002168118260184788	0.0	0.311616456951768	1.185145029683003E-4
78-79	0.0010178304372571673	0.002196004025589094	0.0	0.3134987461165586	1.2548594431937679E-4
80-81	0.0010248018786082438	0.002209946908291247	0.0	0.3139379469216764	1.3245738567045328E-4
82-83	0.0010387447613103968	0.0022238897909934	0.0	0.3143492619613899	1.3942882702152977E-4
84-85	0.0010736019680657792	0.0022448041150466293	0.0	0.31457931952597545	1.3942882702152977E-4
86-87	0.0010736019680657792	0.0023145185285573943	0.0	0.31469783402894375	1.3942882702152977E-4
88	0.0010736019680657792	0.0024539473555789237	0.0	0.31470480547029483	1.3942882702152977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11675	0.0	52.382915	1
GTATCAA	21615	0.0	37.27031	1
TCAACGC	26640	0.0	30.304327	4
ATCAACG	26725	0.0	30.207945	3
CAACGCA	26995	0.0	29.92164	5
TATCAAC	27530	0.0	29.666162	2
AACGCAG	27850	0.0	29.032923	6
TGGTATC	5170	0.0	25.956493	2
GTGGTAT	5380	0.0	25.340508	1
ACGCAGA	31965	0.0	25.30875	7
CGCAGAG	31970	0.0	25.291426	8
GCAGAGT	36520	0.0	22.128683	9
GAGTACT	22930	0.0	21.0978	12-13
TACATGG	23290	0.0	19.909813	2
CAGAGTA	36195	0.0	19.64715	10-11
GTACATG	23995	0.0	19.485138	1
ACATGGG	23400	0.0	19.19525	3
AGAGTAC	34250	0.0	18.735252	10-11
GTACTTT	25820	0.0	18.687086	14-15
CATGGGG	15795	0.0	17.966164	4
>>END_MODULE