FastQCFastQC Report
Fri 27 May 2016
SRR2138743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181772
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117250.049.927531
GTATCAA212300.035.9011651
TCAACGC257500.029.7496264
ATCAACG258050.029.6362133
CAACGCA263000.029.1274855
TATCAAC269000.028.6217082
AACGCAG271600.028.2832896
CTTATAC84300.027.450451
ACGCAGA308100.024.9324877
CGCAGAG316450.024.2746098
TATACAC118850.023.9219783
GCAGAGT368050.020.9648389
TTATACA126750.020.5645622
GTGGTAT56250.020.4930731
TGGTATC54850.020.2319562
GTACATG261550.019.7836171
TACATGG262650.019.5042462
GAGTACT228300.019.42355212-13
ACATGGG262000.018.8795383
CAGAGTA361650.018.82644710-11
Produced by FastQC (version 0.11.5)